Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts

Chemical Reviews, Год журнала: 2018, Номер 118(16), С. 7409 - 7531

Опубликована: Июль 27, 2018

Intracellular delivery is a key step in biological research and has enabled decades of biomedical discoveries. It also becoming increasingly important industrial medical applications ranging from biomanufacture to cell-based therapies. Here, we review techniques for membrane disruption-based intracellular 1911 until the present. These methods achieve rapid, direct, universal almost any cargo molecule or material that can be dispersed solution. We start by covering motivations challenges associated with different types-small molecules, proteins/peptides, nucleic acids, synthetic nanomaterials, large cargo. The then presents broad comparison strategies followed an analysis disruption mechanisms biology cell response. cover mechanical, electrical, thermal, optical, chemical particular emphasis on their implementation. Throughout, highlight specific suggest areas need further experimentation. hope concepts discussed our inspire scientists engineers ideas improve delivery.

Язык: Английский

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Free radical metabolism in human erythrocytes

Clinica Chimica Acta, Год журнала: 2008, Номер 390(1-2), С. 1 - 11

Опубликована: Янв. 22, 2008

Язык: Английский

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Cell or Cell Membrane-Based Drug Delivery Systems

Theranostics, Год журнала: 2015, Номер 5(8), С. 863 - 881

Опубликована: Янв. 1, 2015

Natural cells have been explored as drug carriers for a long period.They received growing interest promising delivery system (DDS) until recently along with the development of biology and medical science.The synthetic materials, either organic or inorganic, are found to be more less immunogenicity and/or toxicity.The extracellular vesicles (EVs), endogenous thought much safer friendlier.Furthermore, in view their host attributes, they may achieve different biological effects targeting specificity, which can meet needs personalized medicine next generation DDS.In this review, we summarized recent progress cell membrane-based DDS fabrication processes, unique properties applications, including whole cells, EVs membrane coated nanoparticles.We expect continuing will promote clinic applications.

Язык: Английский

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Biomimetic proteolipid vesicles for targeting inflamed tissues

Nature Materials, Год журнала: 2016, Номер 15(9), С. 1037 - 1046

Опубликована: Май 23, 2016

Язык: Английский

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Erythrocyte Membrane-Enveloped Polymeric Nanoparticles as Nanovaccine for Induction of Antitumor Immunity against Melanoma

ACS Nano, Год журнала: 2015, Номер 9(7), С. 6918 - 6933

Опубликована: Июль 8, 2015

Cancer immunotherapy is mainly focused on manipulating patient's own immune system to recognize and destroy cancer cells. Vaccine formulations based nanotechnology have been developed target delivery antigens antigen presenting cells (APCs), especially dendritic (DCs) for efficiently induction of antigen-specific T response. To enhance DC targeting efficiency, we erythrocyte membrane-enveloped poly(d,l-lactide-co-glycolide) (PLGA) nanoparticles antigenic peptide (hgp10025-33) toll-like receptor 4 agonist, monophosphoryl lipid (MPLA). A Mannose-inserted membrane structure was constructed actively APCs in the lymphatic organ, redox-sensitive peptide-conjugated PLGA were fabricated which prone cleave intracellular milieu. The nanovaccine demonstrated retained protein content enhanced vitro cell uptake. An antigen-depot effect observed administration site with promoted retention draining lymph nodes. Compared other after intradermal injection, prolonged tumor-occurring time, inhibited tumor growth, suppressed metastasis prophylactic, therapeutic, metastatic melanoma models, respectively. Additionally, revealed that effectively IFN-γ secretion CD8(+) Taken together, these results great potential applying an polymeric nanoplatform immunotherapy.

Язык: Английский

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Multifunctional Theranostic Red Blood Cells For Magnetic‐Field‐Enhanced in vivo Combination Therapy of Cancer

Advanced Materials, Год журнала: 2014, Номер 26(28), С. 4794 - 4802

Опубликована: Май 19, 2014

Red blood cells are attached to iron oxide nanoparticles pre-coated with chlorine e6, a photosensitizer, and then loaded chemotherapeutic drug, doxorubicin, enable imaging-guided combined photodynamic chemotherapy of cancer, achieving excellent synergistic therapeutic effects in an animal tumor model. This work highlights the great promise integrating cell-based drug-delivery systems nanotechnology as biocompatible multifunctional platform for applications cancer theranostics. As service our authors readers, this journal provides supporting information supplied by authors. Such materials peer reviewed may be re-organized online delivery, but not copy-edited or typeset. Technical support issues arising from (other than missing files) should addressed Please note: The publisher is responsible content functionality any Any queries content) directed corresponding author article.

Язык: Английский

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Remotely Controlled Red Blood Cell Carriers for Cancer Targeting and Near‐Infrared Light‐Triggered Drug Release in Combined Photothermal–Chemotherapy

Advanced Functional Materials, Год журнала: 2015, Номер 25(16), С. 2386 - 2394

Опубликована: Фев. 25, 2015

Red blood cells (RBCs), the “innate carriers” in vessels, are gifted with many unique advantages drug transportation over synthetic delivery systems (DDSs). Herein, a tumor angiogenesis targeting, light stimulus‐responsive, RBC‐based DDS is developed by incorporating various functional components within RBC platform. An albumin bound near‐infrared (NIR) dye, together chemotherapy doxorubicin, encapsulated inside RBCs, surfaces of which modified targeting peptide to allow cancer targeting. Under stimulation an external NIR laser, membrane RBCs would be destroyed light‐induced photothermal heating, resulting effective release. As proof principle, cell targeted and light‐controlled release demonstrated vitro, achieving marked synergistic therapeutic effect through combined photothermal–chemotherapy. This work presents novel design smart carriers, inherently biocompatible, promising for combination therapy cancer.

Язык: Английский

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Microalgae-based oral microcarriers for gut microbiota homeostasis and intestinal protection in cancer radiotherapy

Nature Communications, Год журнала: 2022, Номер 13(1)

Опубликована: Март 17, 2022

Abstract Protecting the whole small intestine from radiation-induced intestinal injury during radiotherapy of abdominal or pelvic solid tumors remains an unmet clinical need. Amifostine is a promising selective radioprotector for normal tissues. However, its oral application in radioprotection challenging. Herein, we use microalga Spirulina platensis as microcarrier to construct delivery system. The system shows comprehensive drug accumulation and effective that significantly superior free enteric capsule, preventing prolonging survival without influencing tumor regression. It also benefits on gut microbiota homeostasis long-term safety. Based readily available natural microcarrier, this work presents convenient achieve intestine, providing competitive strategy with great translation potential.

Язык: Английский

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Recent advances in overcoming barriers to cell‐based delivery systems for cancer immunotherapy

Exploration, Год журнала: 2022, Номер 2(3)

Опубликована: Март 15, 2022

Immunotherapy strategies that use cell-based delivery systems have sparked much interest in the treatment of malignancies, owing to their high biocompatibility, excellent tumor targeting capability, and unique biofunctionalities growth process. A variety design principles for immunotherapy, including cell surface decoration, membrane coating, encapsulation, genetically engineered cell, cell-derived exosomes, give cancer immunotherapy great potential improve therapeutic efficacy reduce adverse effects. However, methods is still limited, practical uses are hampered due complex physiological immunological obstacles, such as physical barriers immune infiltration, immunosuppressive microenvironment, upregulation pathways, metabolic restriction. In this review, we present an overview maximize impact, along with anatomical, metabolic, impediments using treat cancer. Following that, a summary novel been created overcome these obstacles immunotherapeutic provided. Also, prospects next-step development concluded end.

Язык: Английский

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Nano-based drug delivery systems: Conventional drug delivery routes, recent developments and future prospects

Medicine in Drug Discovery, Год журнала: 2022, Номер 15, С. 100134 - 100134

Опубликована: Май 20, 2022

Drug delivery can be defined as the group of approaches a drug or pharmacologically active agent transported to target cell treat disease health issue. Conventional routes for are oral, buccal, rectal, subcutaneous, intranasal, intramuscular, intravenous, pulmonary and transdermal. These commonly used methods treatment various medical problems but have certain limitations such instability, risk displacement, uncontrolled release, side effect irritation pain, slow absorption, enzymatic deterioration many others. Incorporation into nanocarrier is one efficient targeted sustained drug. Applications nanocarriers solid nanoparticles, liposomes, dendrimers, polymeric micelles, virus like carbon nanotube mesoporous silica nanoparticles discussed in this review. To overcome drawbacks delivery, innovative systems designed usually termed smart which include nucleic acid-based system, cell-based self-nano emulsifying self-micro chemical physical stimuli-based nanoneedles, patches, ultrasound microchip technology. This article focuses basic mechanism pharmacokinetic study, recent innovations future trends system.

Язык: Английский

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