
Non-coding RNA Research, Год журнала: 2025, Номер unknown
Опубликована: Май 1, 2025
Язык: Английский
Non-coding RNA Research, Год журнала: 2025, Номер unknown
Опубликована: Май 1, 2025
Язык: Английский
Microbiological Research, Год журнала: 2024, Номер 290, С. 127963 - 127963
Опубликована: Ноя. 5, 2024
Язык: Английский
Процитировано
4Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 12, 2025
Язык: Английский
Процитировано
0Biomacromolecules, Год журнала: 2025, Номер unknown
Опубликована: Фев. 24, 2025
Gene therapy holds great promise for treating various diseases, but challenges such as delivery efficiency, immune response, and long-term effects still remain. Protamine is a frequently used gene vector its strong nucleic acid binding capacity, application constrained by inadequate release, resulting in low transfection efficiency. Here, we introduce fusion protein integrating LAH4 peptides on both ends of protamine's DNA-binding motif. This exhibits lower cytotoxicity compared to protamine. At pH 7.4, uniform charge distribution α-helical structure enable robust DNA condensation DNase resistance. Under acidic conditions (pH 5.8), the conformational change weakens binding, facilitating controlled release endosomes/lysosomes. Simultaneously, it interacts with endosomal membrane form pores, aiding escape acids, thereby significantly improving offers potential efficient safe delivery.
Язык: Английский
Процитировано
0Langmuir, Год журнала: 2025, Номер unknown
Опубликована: Март 13, 2025
Ribonucleic acid (RNA) nanocarriers, specifically lipid nanoparticles and polymeric nanoparticles, enable RNA transfection both in vitro vivo; however, only a small percentage of endocytosed by cell is delivered to the cytosolic machinery, minimizing its effect. nanocarriers face two major obstacles after endocytosis: endosomal escape release. Overcoming simultaneously challenging because usually achieved using high positive charge disrupt membrane. However, this typically also inhibits release anionic strongly bound nanocarrier electrostatic interactions. Many address one over other despite growing body evidence demonstrating that are crucial for transfection. In review, we survey various strategies have been employed accomplish with focus on nanomaterials. We first consider requirements must achieve delivery including protection from degradation, cellular internalization, escape, then discuss current polymers used examine achieving Finally, review stimuli-responsive While continues be challenge efficient transfection, many new innovations materials elucidated promising strategies.
Язык: Английский
Процитировано
0Non-coding RNA Research, Год журнала: 2025, Номер unknown
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0