CircRNA-Based AntimiR Therapy: A Novel Approach to Hypertension Treatment DOI Creative Commons
Vahideh Tarhriz, Kamran Hosseini, Leila Abkhooie

и другие.

Non-coding RNA Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Cell-Penetrating Peptides in infection and immunization DOI

Yongliang Du,

Yan Xiong,

Zhou Sha

и другие.

Microbiological Research, Год журнала: 2024, Номер 290, С. 127963 - 127963

Опубликована: Ноя. 5, 2024

Язык: Английский

Процитировано

4

Biomaterial-based vascularization strategies for enhanced treatment of peripheral arterial disease DOI Creative Commons
Haojie Wang,

Fuxin Lin,

Yibin Zhang

и другие.

Journal of Nanobiotechnology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 12, 2025

Язык: Английский

Процитировано

0

pH-Responsive Conformational-Switching Cationic Fusion Protein for Promoted Plasmid DNA Delivery and Transfection DOI

Jun Wu,

Tiantian Tan, Jinghua Chen

и другие.

Biomacromolecules, Год журнала: 2025, Номер unknown

Опубликована: Фев. 24, 2025

Gene therapy holds great promise for treating various diseases, but challenges such as delivery efficiency, immune response, and long-term effects still remain. Protamine is a frequently used gene vector its strong nucleic acid binding capacity, application constrained by inadequate release, resulting in low transfection efficiency. Here, we introduce fusion protein integrating LAH4 peptides on both ends of protamine's DNA-binding motif. This exhibits lower cytotoxicity compared to protamine. At pH 7.4, uniform charge distribution α-helical structure enable robust DNA condensation DNase resistance. Under acidic conditions (pH 5.8), the conformational change weakens binding, facilitating controlled release endosomes/lysosomes. Simultaneously, it interacts with endosomal membrane form pores, aiding escape acids, thereby significantly improving offers potential efficient safe delivery.

Язык: Английский

Процитировано

0

The Interplay of Endosomal Escape and RNA Release from Polymeric Nanoparticles DOI

Timothy H. Cheung,

Molly S. Shoichet

Langmuir, Год журнала: 2025, Номер unknown

Опубликована: Март 13, 2025

Ribonucleic acid (RNA) nanocarriers, specifically lipid nanoparticles and polymeric nanoparticles, enable RNA transfection both in vitro vivo; however, only a small percentage of endocytosed by cell is delivered to the cytosolic machinery, minimizing its effect. nanocarriers face two major obstacles after endocytosis: endosomal escape release. Overcoming simultaneously challenging because usually achieved using high positive charge disrupt membrane. However, this typically also inhibits release anionic strongly bound nanocarrier electrostatic interactions. Many address one over other despite growing body evidence demonstrating that are crucial for transfection. In review, we survey various strategies have been employed accomplish with focus on nanomaterials. We first consider requirements must achieve delivery including protection from degradation, cellular internalization, escape, then discuss current polymers used examine achieving Finally, review stimuli-responsive While continues be challenge efficient transfection, many new innovations materials elucidated promising strategies.

Язык: Английский

Процитировано

0

CircRNA-Based AntimiR Therapy: A Novel Approach to Hypertension Treatment DOI Creative Commons
Vahideh Tarhriz, Kamran Hosseini, Leila Abkhooie

и другие.

Non-coding RNA Research, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0