Dual-Targeting of PDPN-Expressing Synovial Fibroblasts and Macrophages via CLEC-2-Engineered Exosomes for Osteoarthritis Therapy DOI Creative Commons
Bo Yu,

Rui Peng,

Zitao Liu

и другие.

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Ноя. 6, 2024

Abstract Synovitis is often associated with osteoarthritis (OA) and may even precede the onset of OA symptoms. Although targeting synovial inflammation has shown therapeutic promise in OA, synovium's heterogeneous composition, multiple cell types contributing to inflammatory response, indicates that focusing on a single population not provide most favorable results. This investigation employed scRNA-seq tissues from both human murine sources, revealing fibroblasts macrophages expressing high levels Podoplanin (PDPN). These cells constitute approximately 70% total display pro-inflammatory properties. Drawing inspiration unique interaction between PDPN CLEC-2, we engineered mesenchymal stromal cell-derived exosomes overexpress CLEC-2 (ExosomeCLEC-2) encapsulated liquiritigenin-loaded poly (lactic-co-glycolic acid) (PLGA) within ExosomeCLEC-2 membrane (EMCLEC-2), creating PDPN-targeting nanoparticle system called EMCLEC-2-PLGA-liquiritigenin (EMPL). Remarkably, EMPL concurrently targets PDPNhigh macrophages, exhibiting anti-inflammatory effects both vitro in vivo, preventing cartilage degeneration traumatic model. In summary, our research highlights potential developing platform can target mitigate processes offering novel promising strategy for treatment osteoarthritis.

Язык: Английский

Oral barriers to food‐derived active peptides and nano‐delivery strategies DOI
Xinyu Wang, Zhaoxin Cao, Jingyi Su

и другие.

Journal of Food Science, Год журнала: 2025, Номер 90(1)

Опубликована: Янв. 1, 2025

Abstract Food‐derived bioactive peptides are a class of from natural protein. It may have biological effects on the human body and play significant role in protecting physiological health regulating metabolism, such as lowering blood pressure, cholesterol, antioxidant, antibacterial, immune activity, so on. However, most food‐derived functional need to overcome variety barriers enter circulation system target specific tissues generate activity. During this process, bioavailability will be reduced. The nano‐delivery can offer feasibility these obstacles improve stability active by nanoencapsulation. This work summarizes application during delivery pathway vivo. Moreover, different systems used for their were summarized, which could provide ideas oral peptides.

Язык: Английский

Процитировано

0
Dual-targeting of PDPN-expressing synovial fibroblasts and macrophages via CLEC-2-engineered exosomes for osteoarthritis therapy DOI
Bo Yu,

Rui Peng,

Zitao Liu

и другие.

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160628 - 160628

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Nanotherapeutics in COVID-19 and associated pulmonary infections DOI

Zahanat Muneer Mir,

Parul Thakur,

Rimpi Arora

и другие.

Elsevier eBooks, Год журнала: 2025, Номер unknown, С. 371 - 400

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Expanding the horizon of CAR T cell therapy: from cancer treatment to autoimmune diseases and beyond DOI Creative Commons

Zishan Yang,

Boram Ha,

Qinhan Wu

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 19, 2025

Chimeric antigen receptor (CAR)-T-cell therapy has garnered significant attention for its transformative impact on the treatment of hematologic malignancies such as leukemia and lymphoma. Despite remarkable success, challenges resistance, limited efficacy in solid tumors, adverse side effects remain prominent. This review consolidates recent advancements CAR-T-cell explores innovative engineering techniques strategies to overcome immunosuppressive tumor microenvironment (TME). We also discuss emerging applications beyond cancer, including autoimmune diseases chronic infections. Future perspectives highlight development more potent CAR-T cells with increased specificity persistence reduced toxicity, providing a roadmap next-generation immunotherapies.

Язык: Английский

Процитировано

0
Double-Helix Duality: Rods Bow, Toroids Wow in the Nuclease Arena DOI
Yanhua Li, Xiyi Chen,

Changgui Tong

и другие.

The Journal of Physical Chemistry Letters, Год журнала: 2025, Номер unknown, С. 3874 - 3878

Опубликована: Апрель 10, 2025

Utilizing polyion complexation, the formation of rod-like DNA condensates is driven by intrinsic rigidity supramolecular plasmid DNA. Upon interaction with polycationic block copolymers poly(ethylene glycol)-polylysine (PEG-PLys), these macromolecules undergo a regular self-folding process, during which double-stranded (dsDNA) transitions into single-stranded (ssDNA) at kinked junctions. Our investigations, employing transmission electron microscopy (TEM), unprecedentedly reveal absence PEG coating critical junctions, rendering them susceptible to nuclease degradation. This finding underscores necessity for comprehensive encapsulation in engineering robust gene delivery constructs. In stark contrast anisotropic condensates, our novel isotropic toroidal characterized shielding and self-spooling mechanism that preserves dsDNA integrity, exhibit marked enhancement enzymatic stability (nearly 30-fold greater). Their favorable condensation process also confers superior transcriptional potential, positioning as promising platforms next generation systems.

Язык: Английский

Процитировано

0
Changes and application prospects of biomolecular materials in small extracellular vesicles (sEVs) after flavivirus infection DOI Creative Commons

Gengting Du,

He Junhua,

Zhan Yan

и другие.

European journal of medical research, Год журнала: 2025, Номер 30(1)

Опубликована: Апрель 15, 2025

Small extracellular vesicles (sEVs), also known as exosomes, are membranous filled with various proteins and nucleic acids, serving a communication vector between cells. Recent research has highlighted their role in viral diseases. This review synthesizes current understanding of sEVs includes recent findings on infected flaviviruses. It discusses the implications for advancing arbovirus anticipates potential applications flavivirus infections.

Язык: Английский

Процитировано

0
Current multi-scale biomaterials for tissue regeneration following spinal cord injury DOI

Y Zhang,

Zhonghuan Wu,

Junfeng Wu

и другие.

Neurochemistry International, Год журнала: 2024, Номер 178, С. 105801 - 105801

Опубликована: Июль 5, 2024

Язык: Английский

Процитировано

2
Comparative analysis of nucleic acid delivery systems for gene therapy: assessing viral and non-viral approaches with emphasis on extracellular vesicles DOI Creative Commons
Nizar Y. Saad

Deleted Journal, Год журнала: 2024, Номер 01(02), С. 67 - 81

Опубликована: Март 4, 2024

In the landscape of modern medicine, nucleic acid therapeutics have emerged as groundbreaking agents, holding promise to revolutionize disease treatment at its genetic roots.These encompass a spectrum approaches, including gene, oligonucleotide drug, and mRNA therapies, each designed modulate gene expression or function.The potential lies in their ability address disorders by directly targeting manipulating underlying material.However, translation therapeutic concepts into effective clinical interventions faces challenges, spanning delivery hurdles, immunogenicity concerns, need for sustained efficacy.This Commentary article navigates complexities associated with therapeutics, particular focus on therapy, explores recent emergence extracellular vesicles solution overcome hurdles delivery.

Язык: Английский

Процитировано

1
生物启发和生物工程核酸药物载体 DOI

Jiamin Zuo,

Zhiguo Lü, Jing Guo

и другие.

Science China Materials, Год журнала: 2024, Номер 67(8), С. 2393 - 2413

Опубликована: Июль 12, 2024

Процитировано

1
Hydrogen Sulfide‐Triggered Artificial DNAzyme Switches for Precise Manipulation of Cellular Functions DOI
Xuan Xie,

Hexin Nan,

Jialong Peng

и другие.

Angewandte Chemie, Год журнала: 2024, Номер 136(49)

Опубликована: Сен. 27, 2024

Abstract The development of synthetic molecular tools responsive to biological cues is crucial for advancing targeted cellular regulation. A significant challenge the regulation processes in response gaseous signaling molecules such as hydrogen sulfide (H 2 S). To address this, we present design Gas molecule‐Responsive Artificial DNAzyme‐based Switches (GRAS) manipulate functions via H S‐sensitive DNAzymes. By incorporating stimuli‐responsive moieties phosphorothioate backbone, DNAzymes are strategically designed with S‐responsive azide groups at cofactor binding locations within catalytic core region. These modifications enable their activation through S‐reducing decaging, thereby initiating substrate cleavage activity. Our approach allows flexible customization various regulate distinct diverse scenarios. Intracellularly, enzymatic activity GRAS promotes S‐induced specific mRNA sequences, enabling gene silencing and inducing apoptosis cancer cells. Moreover, integrating dynamic DNA assembly grafting these functional switches onto cell surface receptors, facilitating S‐triggered receptor dimerization. This extracellular transmits signals intracellularly behaviors migration proliferation. Collectively, capable rewiring cues, offering a promising avenue advanced engineering.

Язык: Английский

Процитировано

1