Core-shell vector-mediated co-delivery of CRISPR/Cas9 system and hydrophobic drugs against triple-negative breast cancer stem cells
Journal of Controlled Release,
Год журнала:
2025,
Номер
378, С. 1080 - 1091
Опубликована: Янв. 5, 2025
Язык: Английский
Targeted delivery of extracellular vesicles: the mechanisms, techniques and therapeutic applications
Molecular Biomedicine,
Год журнала:
2024,
Номер
5(1)
Опубликована: Ноя. 21, 2024
Abstract
Extracellular
vesicles
(EVs)
are
cell-derived
with
a
phospholipid
bilayer
measuring
50–150
nm
in
diameter
demonstrated
therapeutic
potentials.
Limitations
such
as
the
natural
biodistribution
(mainly
concentrated
liver
and
spleen)
short
plasma
half-life
of
EVs
present
significant
challenges
to
their
clinical
translation.
In
recent
years,
growing
research
indicated
that
engineered
enhanced
targeting
lesion
sites
have
markedly
promoted
efficacy.
However,
there
is
dearth
systematic
knowledge
on
advances
engineering
for
targeted
delivery.
Herein,
we
provide
an
overview
mechanisms,
techniques,
translations
applications.
Enrichment
at
may
be
achieved
through
recognition
tissue
markers,
pathological
changes,
circumvention
mononuclear
phagocyte
system
(MPS).
Alternatively,
external
stimuli,
including
magnetic
fields
ultrasound,
also
employed.
EV
techniques
fulfill
functions
includes
genetic
engineering,
membrane
fusion,
chemical
modification
physical
modification.
A
comparative
statistical
analysis
was
conducted
elucidate
discrepancies
between
diverse
size,
morphology,
stability,
efficacy
vitro
vivo.
Additionally,
summary
registered
trials
utilizing
from
2010
2023
has
been
provided,
full
discussion
perspectives.
This
review
provides
comprehensive
mechanisms
associated
delivery
applications
advocate
further
explorations
accelerate
Язык: Английский
Beneficial and challenges of exosome application in ischemic heart disease
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Май 19, 2025
Cardiovascular
diseases
are
the
main
cause
of
death
and
disability
in
clinical
setting.
Among
several
pathological
conditions,
myocardial
infarction
(MI)
is
a
common
finding
happens
due
to
reduction
or
complete
interruption
blood
support.
Stem
cells
progenitors
valid
cell
sources
with
significant
potential
alleviate
tissue
injuries.
Differentiation
mature
functional
release
various
growth
factors,
cytokines
reparative
mechanisms
by
which
stem
mediate
their
tasks.
Exosomes
(Exos),
subset
extracellular
vesicles
(EVs),
exhibit
great
theranostic
biomedicine.
Along
whole-cell-based
therapies,
pre-clinical
application
Exos
has
been
extended
animals
humans
ischemic
heart
(IHD).
Here,
this
review
article,
we
aimed
highlight
importance
IHD
address
mechanism
action
focusing
on
regenerative
potential.
Язык: Английский
Nanodelivery of nucleic acids for plant genetic engineering
Discover Nano,
Год журнала:
2025,
Номер
20(1)
Опубликована: Фев. 12, 2025
Genetic
engineering
in
plants
serves
as
a
crucial
method
for
enhancing
crop
quality,
yield,
and
climate
resilience
through
the
manipulation
of
genetic
circuits.
A
novel
transformation
approach
utilizing
nanocarriers
sound
plant
technique
enables
delivery
DNAs
or
RNAs
into
cells.
Significant
advances
have
recently
been
made
on
nanotechnology-based
nucleic
acids
plants.
In
this
review,
several
nanoparticle-mediated
DNA
RNA
systems
are
discussed
respectively,
involving
latest
progresses
drawbacks
these
approaches
used
engineering.
We
also
underscores
current
challenges
that
must
be
addressed
implementation
nanoparticles-based
strategies
gene
delivery.
Furthermore
more
importantly,
plant-derived
exosome-like
nanoparticles
facilitate
transfer
between
organisms
was
initially
proposed
promising
nanodelivery
platform
CRISPR/Cas9
genome
editing
toolkit
believe
review
will
beneficial
an
effective
exploration
acid
to
aid
modern
agriculture.
Язык: Английский
Trojan Horse-Like Vehicles for CRISPR-Cas Delivery: Engineering Extracellular Vesicles and Virus-Like Particles for Precision Gene Editing in Cystic Fibrosis
Human Gene Therapy,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 28, 2025
The
advent
of
genome
editing
has
kindled
the
hope
to
cure
previously
uncurable,
life-threatening
genetic
diseases.
However,
whether
this
promise
can
be
ultimately
fulfilled
depends
on
how
efficiently
gene
agents
delivered
therapeutically
relevant
cells.
Over
time,
viruses
have
evolved
into
sophisticated,
versatile,
and
biocompatible
nanomachines
that
engineered
shuttle
payloads
specific
cell
types.
Despite
advances
in
safety
selectivity,
long-term
expression
sustained
by
viral
vectors
remains
a
cause
for
concern.
Cell-derived
vesicles
(CDVs)
are
gaining
traction
as
elegant
alternatives.
CDVs
encompass
extracellular
(EVs),
diverse
set
intrinsically
low-immunogenic
membranous
nanoparticles,
virus-like
particles
(VLPs),
bioparticles
with
scaffold
envelope
structures,
but
devoid
material.
Both
EVs
VLPs
deliver
ribonucleoprotein
cargo
target
cytoplasm,
ensuring
machinery
is
only
transiently
active
thereby
increasing
its
safety.
In
review,
we
explore
natural
diversity
their
potential
delivery
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
machinery.
We
illustrate
different
strategies
optimization
CDV
loading
retargeting,
highlighting
versatility
tunability
these
vehicles.
Nonetheless,
lack
robust
standardized
protocols
production,
purification,
quality
assessment
still
hinders
widespread
adoption
further
CRISPR-based
therapies
advanced
"living
drugs."
believe
collective,
multifaceted
effort
urgently
needed
address
critical
issues
unlock
full
genome-editing
technologies
yield
safe,
easy-to-manufacture,
pharmacologically
well-defined
therapies.
Finally,
discuss
current
clinical
landscape
lung-directed
cystic
fibrosis
could
drive
significant
breakthroughs
vivo
disease.
Язык: Английский
Recent advances in the roles of extracellular vesicles in cardiovascular diseases: pathophysiological mechanisms, biomarkers, and cell-free therapeutic strategy
Molecular Medicine,
Год журнала:
2025,
Номер
31(1)
Опубликована: Май 5, 2025
Abstract
Cardiovascular
diseases
(CVDs)
represent
a
profound
challenge
with
inflammation
playing
significant
role
in
their
pathophysiology.
Extracellular
vesicles
(EVs),
which
are
membranous
structures
encapsulated
by
lipid
bilayer,
essential
for
intercellular
communication
facilitating
the
transport
of
specific
bioactive
molecules,
including
microRNAs,
proteins,
and
lipids.
Emerging
evidence
suggests
that
regulatory
mechanisms
governing
cardiac
resident
cells
influenced
EVs,
function
as
messengers
thereby
contribute
to
advancement
CVDs.
In
this
review,
we
discuss
multifaceted
biological
functions
EVs
involvement
pathogenesis
various
CVDs,
encompassing
myocardial
infarction,
ischemia–reperfusion
injury,
heart
failure,
atherosclerosis,
myocarditis,
cardiomyopathy,
aneurysm.
Furthermore,
summarize
recent
advancements
utilizing
non-invasive
biomarkers
cell-free
therapy
based
on
diagnosis
treatment
Future
research
should
investigate
effective
techniques
isolation
purification
from
body
fluids,
while
also
exploring
pathways
clinical
translation
EVs.
Additionally,
it
is
imperative
identify
appropriate
EV-miRNA
profiles
or
combinations
present
circulation
patients,
could
serve
improve
diagnostic
accuracy
By
synthesizing
integrating
findings,
review
aims
provide
innovative
perspectives
CVDs
potential
therapeutic
strategies.
Язык: Английский
CRISPR-based genetically modified scaffold-free biomaterials for tissue engineering and regenerative medicine
Biomaterials Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
This
review
focuses
on
CRISPR-based
genetically
modified
extracellular
vehicles,
cell
sheets,
aggregates,
organoids,
and
organs,
elaborating
their
applications
future
directions
in
tissue
engineering
regenerative
medicine.
Язык: Английский
Engineering extracellular vesicles for targeted therapeutics in cardiovascular disease
Frontiers in Cardiovascular Medicine,
Год журнала:
2024,
Номер
11
Опубликована: Дек. 19, 2024
Extracellular
vesicles
(EVs)
are
nanosized
particles
secreted
by
cells
that
play
crucial
roles
in
intercellular
communication,
especially
the
context
of
cardiovascular
diseases
(CVDs).
These
carry
complex
cargo,
including
proteins,
lipids,
and
nucleic
acids,
reflects
physiological
or
pathological
state
their
origin.
Multiomics
analysis
cell-derived
EVs
has
provided
valuable
insights
into
molecular
mechanisms
underlying
CVDs
identifying
specific
proteins
EV-bound
targets
involved
disease
progression.
Recent
studies
have
demonstrated
engineered
EVs,
which
designed
to
therapeutic
molecules
modified
enhance
targeting
capabilities,
hold
promise
for
treating
CVDs.
Analysis
EV
proteome
been
instrumental
key
can
be
targeted
modulated
within
these
vesicles.
For
example,
inflammation,
thrombosis,
cardiac
remodeling
identified
as
potential
targets.
Furthermore,
engineering
increase
delivery
tissues,
such
myocardium,
modulate
immunogenicity
efficacy
is
an
emerging
area
research.
By
leveraging
gained
from
multiomics
analyses,
researchers
developing
EV-based
therapies
selectively
target
processes
CVDs,
offering
a
novel
potentially
more
effective
treatment
strategy.
This
review
integrates
core
findings
highlights
applications.
Язык: Английский