Journal of Biomaterials Science Polymer Edition,
Год журнала:
2024,
Номер
unknown, С. 1 - 20
Опубликована: Окт. 16, 2024
Nanoscale
drug
delivery
systems
that
are
both
multifunctional
and
targeted
have
been
developed
using
proteins
as
a
basis,
thanks
to
their
attractive
biomacromolecule
properties.
A
novel
nanocarrier,
aptamer
(AS1411)-conjugated
β-lactoglobulin/poly-l-lysine
(BLG/Ap/PL)
nanoparticles,
was
in
this
study.
To
unique
formulation,
the
as-prepared
nanocarrier
blends
distinctive
features
of
an
chemotherapeutic
targeting
agent
with
those
protein
nanocarriers.
By
loading
cabazitaxel
(CTX)
onto
nanocarriers,
therapeutic
potential
BLG/Ap/PL
could
be
demonstrated.
The
CTX-loaded
(CTX@BLG/Ap/PL)
showed
regulated
release
profile
acidic
milieu,
which
improve
efficacy
cancer
cells
high
encapsulation
up
93%.
However,
compared
free
CTX,
CTX@BLG/Ap/PL
killed
colorectal
HCT116
higher
at
24
48
h.
Further
investigation
confirms
apoptosis
by
acridine
orange
ethidium
bromide
(AO/EB),
DAPI
staining
morphological
changes,
chromatin
condensation,
membrane
blebbing
treated
cell
through
flow
cytometry
displayed
percentages
apoptosis.
Cell
cycle
analysis
revealed
induced
sub-G1
G2/M
phase
(apoptosis)
Annexin
V/propidium
iodide
(PI)
confirmed
induces
cells.
Overall,
study
proved
had
several
advantages
over
drugs
promise
solution
clinical
problems
associated
antitumor
systems.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Апрель 3, 2024
This
study
examines
the
manufacturing,
characterization,
and
biological
evaluation
of
platinum
nanoparticles,
which
were
synthesized
by
Enterobacter
cloacae
coated
with
Bovine
Serum
Albumin
(BSA)
Resveratrol
(RSV).
The
formation
PtNPs
was
confirmed
change
color
from
dark
yellow
to
black,
due
bioreduction
chloride
E.
cloacae.
BSA
RSV
functionalization
enhanced
these
nanoparticles'
biocompatibility
therapeutic
potential.
TGA,
SEM,
XRD,
FTIR
employed
for
where
drug
conjugation-related
functional
groups
studied
FTIR.
XRD
crystalline
nature
Pt-BSA-RSV
NPs,
while
TGA
SEM
showed
thermal
stability
post-drug
coating
morphological
changes.
Designed
composite
also
found
be
biocompatible
in
hemolytic
testing,
indicating
their
potential
Biomedical
applications.
After
confirmation
based
nanocaompsite
synthesis,
they
examined
anti-bacterial,
anti-oxidant,
anti-inflammatory,
anti-cancer
properties.
NPs
higher
concentration-dependent
DPPH
scavenging
activity,
measured
antioxidant
capability.
Enzyme
inhibition
tests
demonstrated
considerable
anti-inflammatory
activity
against
COX-2
15-LOX
enzymes.
In
vitro
anticancer
studies,
effectively
killed
human
ovarian
cancer
cells.
phenomenon
facilitated
acidic
environment
cancer,
as
release
assay
NP
formulation
environment.
Finally,
Molecular
docking
that
has
strong
an
antibacterial,
agent.
Overall,
silico
investigations
current
good
medicinal
applications
designed
nanocomposites,
however,
further
in-vivo
experiments
must
conducted
validate
our
findings.
Macromolecular Bioscience,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 30, 2025
Abstract
Chemotherapy
is
generally
given
by
intravenous
(IV)
administration
which
provides
higher
bioavailability
than
other
systemic
routes.
However,
in
the
case
of
lung
cancer,
pulmonary
(INH)
route
choice
for
inhalable
formulations.
In
study,
biochemical
and
histological
parameters
Cabazitaxel
(CBZ)
free
(2
mg
kg
−1
)
nanoparticle
(NP)
CBZ
equivalent)
formulations
are
investigated
after
IV
INH
rats.
The
nanoformulation
obtained
using
PEGylated
polystyrene
(PEG‐PST)
nanoparticles
PISA.
While
a
nose
head‐only
device
used
administration,
jugular
vein
as
route.
Blood
samples
(blank,
24
h,
48
h)
collected
via
carotid
artery
cannulas
without
handling
metabolism
cages.
According
to
parameters,
formulation
applied
or
shows
toxicity.
On
hand,
showed
no
signs
toxicity
both
Higher
longer
retention
observed
inhaled
nanoformulation.
Histological
analysis
alveolar
macrophage
migration
due
enhanced
retention.
Results
that
nanotechnology
defense
system
gave
advantage
end
up
with
an
nanomedicine
cancer.
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 3009 - 3029
Опубликована: Март 1, 2024
Background:
Biodegradable
poly(alkyl
cyanoacrylate)
(PACA)
nanoparticles
(NPs)
are
receiving
increasing
attention
in
anti-cancer
nanomedicine
development
not
only
for
targeted
cancer
chemotherapy,
but
also
modulation
of
the
tumor
microenvironment.
We
previously
reported
promising
results
with
cabazitaxel
(CBZ)
loaded
poly(2-ethylbutyl
NPs
(PEBCA-CBZ
NPs)
a
patient
derived
xenograft
(PDX)
model
triple-negative
breast
cancer,
and
this
was
associated
decrease
M2
macrophages.
The
present
study
aims
at
comparing
two
endotoxin-free
PACA
NP
variants
(PEBCA
poly(2-ethylhexyl
cyanoacrylate);
PEHCA),
CBZ
test
whether
conjugation
folate
would
improve
their
effect.
Methods:
Cytotoxicity
assays
cellular
uptake
by
flow
cytometry
were
performed
different
cells.
Biodistribution
efficacy
studies
PDX
models
cancer.
Tumor
immune
cells
analyzed
multiparametric
cytometry.
Results:
In
vitro
showed
similar
NP-induced
cytotoxicity
patterns
despite
difference
early
internalization.
On
intravenous
injection,
liver
cleared
majority
NPs.
Efficacy
HBCx39
demonstrated
an
enhanced
effect
drug-loaded
PEBCA
compared
free
drug
PEHCA
Furthermore,
conjugated
variant
did
show
any
effects
unconjugated
counterpart
which
might
be
due
to
unfavorable
orientation
on
Finally,
analyses
cell
populations
tumors
revealed
that
treatment
affected
myeloid
cells,
especially
macrophages,
contributing
inflammatory,
activated
Conclusion:
report
first
time,
comparative
triple
negative
CBZ-loaded
exhibit
combined
compartment,
may
boost
anti-tumor
response.
Keywords:
cyanoacrylate),
cabazitaxel,
patient-derived
xenograft,
microenvironment
