Preparation and Characterization of Tiamulin-Loaded Niosomes for Oral Bioavailability Enhancement in Mycoplasma-Infected Broilers DOI Creative Commons

Shimaa G. Abonashey,

Amr Gamal, Hatem A. F. M. Hassan

и другие.

Micro, Год журнала: 2024, Номер 4(4), С. 734 - 750

Опубликована: Ноя. 27, 2024

Mycoplasma infections pose significant challenges in the poultry industry, necessitating effective therapeutic interventions. Tiamulin, a veterinary antibiotic, has demonstrated efficacy against species. However, emergence of resistant species could dramatically reduce potential, contributing to economic losses. Optimizing tiamulin’s pharmacokinetic profile via nanocarrier incorporation enhance its potential and administration frequency, ultimately reducing strain emergence. Niosomes, type self-assembled non-ionic surfactant-based nanocarrier, have emerged as promising drug delivery system, offering improved stability, sustained release, enhanced bioavailability. In this study, niosomal nanocarriers encapsulating tiamulin were prepared, characterized assessed Mycoplasma-inoculated broilers following oral administration. Differential scanning colorimetry (DSC) confirmed alterations crystalline state components integration into structures formed during formulation procedure. Transmission electron microscopy (TEM) showed spherical nanostructure niosomes. The formulated exhibited zeta average hydrodynamic diameter −10.65 ± 1.37 mV 339.67 30.88 nm, respectively. Assessment parameters gallisepticum-infected revealed ability increase bioavailability systemic exposure, marked by significantly higher area under curve (AUC) (p < 0.01) prolonged elimination half-life (T1/2) 0.05). Enhanced residence time are crucial factors maintaining concentrations at reduced doses frequencies. This approach provides viable strategy decrease risk subtherapeutic levels, thereby mitigating development antibiotic resistance. findings presented herein offer sustainable for efficient use antibiotics medicine.

Язык: Английский

Facile sonochemically-assisted bioengineering of titanium dioxide nanoparticles and deciphering their potential in treating breast and lung cancers: biological, molecular, and computational-based investigations DOI Creative Commons
Nada K. Sedky, Noha Khalil Mahdy, Nour M. Abdel-Kader

и другие.

RSC Advances, Год журнала: 2024, Номер 14(12), С. 8583 - 8601

Опубликована: Янв. 1, 2024

Combining sonochemistry with phytochemistry is a modern trend in the biosynthesis of metallic nanoparticles (NPs), which contributes to sustainability chemical processes and minimizes hazardous effects.

Язык: Английский

Процитировано

11
Exploring the potential of silymarin-loaded nanovesicles as an effective drug delivery system for cancer therapy: in vivo, in vitro, and in silico experiments DOI
Mohammad Reza Hajinezhad,

Maryam Roostaee,

Zahra Nikfarjam

и другие.

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер 397(9), С. 7017 - 7036

Опубликована: Апрель 17, 2024

Язык: Английский

Процитировано

10
Emerging tendencies for the nano-delivery of gambogic acid: a promising approach in oncotherapy DOI Creative Commons
Sherif Ashraf Fahmy,

Rawan Elghanam,

Gowhar Rashid

и другие.

RSC Advances, Год журнала: 2024, Номер 14(7), С. 4666 - 4691

Опубликована: Янв. 1, 2024

Despite the advancements in cancer therapies during past few years, chemo/photo resistance, severe toxic effects, recurrence of metastatic tumors, and non-selective targeting remain incomprehensible.

Язык: Английский

Процитировано

9
Synergistic anticancer effect of Pistacia lentiscus essential oils and 5-Fluorouracil co-loaded onto biodegradable nanofibers against melanoma and breast cancer DOI Creative Commons
Obaydah Abd Alkader Alabrahim, Hassan Mohamed El-Said Azzazy

Discover Nano, Год журнала: 2024, Номер 19(1)

Опубликована: Фев. 14, 2024

Abstract Chemoresistance and severe toxicities represent major drawbacks of chemotherapy. Natural extracts, including the essential oils Pistacia lentiscus (PLEO), exhibit substantial anticancer anti-inflammatory activities where different cancers are reported to dramatically recess following targeting with PLEO. PLEO has promising antimicrobial, anticancer, properties. However, therapeutic properties restricted by limited stability, bioavailability, ability. nanoformulation can maximize their physicochemical properties, overcoming shortcomings. Hence, was extracted its chemical composition determined GC–MS. 5-Fluorouracil (5FU) were electrospun into poly-ε-caprolactone nanofibers (PCL-NFs), 290.71 nm 680.95 diameter, investigate potential synergistic against triple-negative breast cancer cells (MDA-MB-231), human adenocarcinoma (MCF-7), skin melanoma cell line (A375). The prepared (NFs) showed enhanced thermal stability remarkable physical integrity tensile strength. Biodegradability studies prolonged over 42 days, supporting NFs use as a localized therapy tissues (postmastectomy) or melanoma. Release revealed sustainable release behaviors 168 h, higher released amounts 5FU at pH 5.4, indicating abilities towards tissues. loaded strong antioxidant Finally, either depicted greater compared free compounds. highest observed co-loaded 5FU. developed 5FU-PLEO-PCL-NFs hold local treatment (post-mastectomy) minimize possible recurrence. Graphical

Язык: Английский

Процитировано

9
Deploying nucleic acids-loaded plant-derived exosomes as green nano gadget in cancer gene therapy DOI Creative Commons

Marola Paula Fawzy,

Hatem A. F. M. Hassan, Muhammad Umair Amin

и другие.

Materials Advances, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

The pursuit of effective drug delivery systems is critical in advancing cancer therapies, particularly the realms chemotherapy, radiotherapy and immunotherapy.

Язык: Английский

Процитировано

1
Box–Behnken design of thermo-responsive nano-liposomes loaded with a platinum(iv) anticancer complex: evaluation of cytotoxicity and apoptotic pathways in triple negative breast cancer cells DOI Creative Commons
Nada K. Sedky, Maria Braoudaki, Noha Khalil Mahdy

и другие.

Nanoscale Advances, Год журнала: 2023, Номер 5(19), С. 5399 - 5413

Опубликована: Янв. 1, 2023

Herein, thermo-responsive liposomes (TLs) loaded with Asp (Asp/TLs) were produced by self-assembling DPPC, DSPE-PEG2000, and cholesterol. The preparation variables optimized using the Box-Behnken design (BBD). Asp/TLs exhibited an average particle size of 114.05 ± 1.56 nm, PDI 0.15 0.015, zeta potential -15.24 0.65 mV, entrapment efficiency (EE%) 84.08 2.75%. In addition, under physiological conditions, showed spherical shape, outstanding stability thermo-triggered release at 38 °C, reaching maximum 40 °C. MTT assay that optimal highest cytotoxic activity upon exposure to mild hyperthermia (40 °C) against invasive triple-negative breast cancer cell line (MDA-MB-231) when compared other preparations. IC50 was estimated 0.9 μg mL-1, while free 3.83 mL-1. As such, shown increase 4-fold hyperthermia. values without °C 6.6 mL-1 186 respectively. This indicated released only placed apoptosis revealed caused a remarkable in percentage population among both late necrosis quartiles, as well significant decline viable quartile (P ≤ 0.001) °C). could stimulate intrinsic pathway upregulating apoptotic genes Bak Bax, downregulating anti-apoptotic genes, BCL-xL BCL-2. increased gene expression Bax 4.4- 5.2-folds, reducing BCL-2 50% 73%, TLs manifested more potent effects demonstrated upregulation Bak, P53 5.6-, 7.2-, 1.3-folds, downregulation 70% 85%, treatment displayed most profile induced MDA-MB-231.

Язык: Английский

Процитировано

21
Innovative microwave-assisted biosynthesis of copper oxide nanoparticles loaded with platinum(ii) based complex for halting colon cancer: cellular, molecular, and computational investigations DOI Creative Commons
Nada K. Sedky,

Iten M. Fawzy,

Afnan Hassan

и другие.

RSC Advances, Год журнала: 2024, Номер 14(6), С. 4005 - 4024

Опубликована: Янв. 1, 2024

In the current study, we biosynthesized copper oxide NPs (CuO NPs) utilizing essential oils extracted from Boswellia carterii oleogum resin, which served as a bioreductant and capping agent with help of microwave energy.

Язык: Английский

Процитировано

7
Revolutionizing cancer therapy: nanoformulation of miRNA-34 – enhancing delivery and efficacy for various cancer immunotherapies: a review DOI Creative Commons

Marola Paula Fawzy,

Hatem A. F. M. Hassan, Nada K. Sedky

и другие.

Nanoscale Advances, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Despite recent advancements in cancer therapies, challenges such as severe toxic effects, non-selective targeting, resistance to chemotherapy and radiotherapy, recurrence of metastatic tumors persist. Consequently, there has been considerable effort explore innovative anticancer compounds, particularly immunotherapy, which offer the potential for enhanced biosafety efficacy prevention treatment. One avenue exploration involves miRNA-34 (miR-34) family, known its ability inhibit tumorigenesis across various cancers. Dysregulation miR-34 observed several human cancers, it is recognized a tumor suppressor microRNA due synergistic interaction with well-established p53. However, have arisen therapeutic application miR-34a. These include susceptibility degradation by RNase serum, limiting penetrate capillary endothelium reach target cells, well reports immunoreactive adverse reactions. Furthermore, unexpected side effects may occur, accumulation miRNAs healthy tissues interactions serum proteins on nano-vector surfaces, nanoparticle breakdown bloodstream shearing stress, unsuccessful extravasation nanocarriers cells owing interstitial fluid pressure. these challenges, miR-34a remains promising candidate therapy, other members family also shown inhibiting cell proliferation. While

Язык: Английский

Процитировано

6
Recent Innovations of Mesoporous Silica Nanoparticles Combined with Photodynamic Therapy for Improving Cancer Treatment DOI Creative Commons

Doaa Sayed Nady,

Afnan Hassan, Muhammad Umair Amin

и другие.

Pharmaceutics, Год журнала: 2023, Номер 16(1), С. 14 - 14

Опубликована: Дек. 20, 2023

Cancer is a global health burden and one of the leading causes death. Photodynamic therapy (PDT) considered an alternative approach to conventional cancer treatment. PDT utilizes light-sensitive compound, photosensitizers (PSs), light irradiation, molecular oxygen (O2). This generates cytotoxic reactive species (ROS), which can trigger necrosis and/ or apoptosis, cell death in intended tissues. Classical impose limitations that hinder their clinical applications, such as long-term skin photosensitivity, hydrophobic nature, nonspecific targeting, toxic cumulative effects. Thus, nanotechnology emerged unorthodox solution for improving hydrophilicity targeting efficiency PSs. Among nanocarriers, mesoporous silica nanoparticles (MSNs) have gained increasing attention due high surface area, defined pore size structure, ease modification, stable aqueous dispersions, good biocompatibility, optical transparency, are vital PDT. The advancement integrated MSNs/PDT has led inspiring multimodal nanosystem effectively treating malignancies. review gives overview main components mechanisms process, effect on tumor cells, most recent studies reported benefits incorporating PSs into integration with against different cells.

Язык: Английский

Процитировано

11
Hijacking 5-Fluorouracil Chemoresistance in Triple Negative Breast Cancer via microRNAs-Loaded Chitosan Nanoparticles DOI Open Access
Sherif Ashraf Fahmy, Noha Khalil Mahdy,

Adham H. Mohamed

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2070 - 2070

Опубликована: Фев. 8, 2024

Chemotherapy is still the mainstay of treatment for triple-negative breast cancer (TNBC) patients. Yet only 20% TNBC patients show a pathologic complete response (pCR) after neoadjuvant chemotherapy. 5-Fluorouracil (5-FU) stable cornerstone in all recommended chemotherapeutic protocols However, patients’ innate or acquired chemoresistance rate 5-FU steeply escalating. This study aims to unravel mechanism behind aggressive cell line, MDA-MB-231 cells, explore further role tumor suppressor microRNAs (miRNAs), miR-1275, miR-615-5p, and Let-7i, relieving TNBC, finally provide translational therapeutic approach co-deliver respective miRNA oligonucleotides using chitosan-based nanoparticles (CsNPs). In this regard, cellular viability proliferation were investigated MTT BrdU assays, respectively. was found induce JAK/STAT PI3K/Akt/mTOR pathways cells with contaminant repression their upstream regulators Let-7i. Moreover, CsNPs prepared ionic gelation method chosen studied as nanovectors combination targeting TNBC. The average particle sizes, surface charges, morphologies different characterized dynamic light scattering (DLS) transmission electron microscopy (TEM), addition, encapsulation efficiency (EE%), drug loading capacity (DLC%), release manner at two pH values assessed. conclusion, novel co-loaded three demonstrated synergistic activity remarkable through alleviating 5-FU.

Язык: Английский

Процитировано

4