Resveratrol-Ampicillin Dual-Drug Loaded Polyvinylpyrrolidone/Polyvinyl Alcohol Biomimic Electrospun Nanofiber Enriched with Collagen for Efficient Burn Wound Repair
International Journal of Nanomedicine,
Год журнала:
2024,
Номер
Volume 19, С. 5397 - 5418
Опубликована: Июнь 1, 2024
Background:
The
healing
of
burn
wounds
is
a
complicated
physiological
process
that
involves
several
stages,
including
haemostasis,
inflammation,
proliferation,
and
remodelling
to
rebuild
the
skin
subcutaneous
tissue
integrity.
Recent
advancements
in
nanomaterials,
especially
nanofibers,
have
opened
new
way
for
efficient
due
burning
or
other
injuries.
Methods:
This
study
aims
develop
characterize
collagen-decorated,
bilayered
electrospun
nanofibrous
mats
composed
PVP
PVA
loaded
with
Resveratrol
(RSV)
Ampicillin
(AMP)
accelerate
wound
repair.
Results:
Nanofibers
smooth
surfaces
web-like
structures
diameters
ranging
from
200
400
nm
were
successfully
produced
by
electrospinning.
These
fibres
exhibited
excellent
vitro
properties,
ability
absorb
exudates
undergo
biodegradation
over
two-week
period.
Additionally,
these
nanofibers
demonstrated
sustained
controlled
release
encapsulated
through
studies.
zone
inhibition
(ZOI)
PVP-PVA-RSV-AMP
against
Staphylococcus
aureus
(
S.
aureus)
Escherichia
coli
E.
)
was
found
31±
0.09
mm
12±
0.03,
respectively,
which
significantly
higher
as
compared
positive
control.
Similarly,
biofilm
confirmed
significant
reduction
formation
biofilms
nanofiber-treated
group
both
.
X-ray
diffraction
(XRD)
Fourier
transform
infrared
spectroscopy
(FTIR)
analysis
proved
encapsulation
RSV
AMP
into
their
compatibility.
Haemolysis
assay
(%)
showed
no
haemolysis
(less
than
5%)
groups,
cytocompatibility
red
blood
cells
(RBCs).
Cell
viability
cell
adhesion
on
HaCaT
increased
indicating
its
biocompatibility
well
non-toxic
properties.
Results
in-vivo
experiments
model
potential
rats
H&E-stained
images
also
improved
collagen
synthesis
nanofibers-treated
groups
evidenced
Masson-trichrome
staining.
ELISA
clearly
indicated
downregulation
TNF-alpha
IL-6
inflammatory
biomarkers
after
treatment
day
10.
Conclusion:
AMP-loaded
nanofiber
mats,
developed
this
study,
expedite
multifaceted
approach.
Keywords:
electrospinning,
nanofiber,
resveratrol,
ampicillin,
healing,
Язык: Английский
Dual-ligand functionalized liposomes with iRGD/trastuzumab co-loaded with gefitinib and lycorine for enhanced metastatic breast cancer therapy
Journal of Liposome Research,
Год журнала:
2025,
Номер
unknown, С. 1 - 15
Опубликована: Фев. 2, 2025
Personalized
treatment
strategies
have
greatly
improved
the
efficacy
of
anticancer
drugs.
Nanocarriers,
especially
liposomes,
function
as
excellent
platform
for
delivery
both
hydrophilic
and
hydrophobic
agents.
iRGD
is
a
peptide
composed
9-amino
acid
denoted
(iRGDP),
enhances
selective
intratumoral
Trastuzumab
(TMAB),
mainly
targets
HER2-positive
advanced
stage
breast
cancer
an
FDA-approved
monoclonal
antibody.
Gefitinib
(GEB)
drug,
effective
against
metastatic
(MBC),
while
Lycorine
hydrochloride
(LCOH),
naturally
derived
compound,
possess
anti-inflammatory
properties.
This
research
emphasizing
on
preparation
GEB
LCOH-entrapped
TPGS-COOH
coated-liposomes,
camouflaged
with
antibody
(TMAB)
cyclic
(iRGDP)
targeted
in
MBC
therapy.
The
developed
multifunctional
liposomes
were
studied
extensive
vitro
cell
line
studies
MCF-7
cells.
half-maximum
inhibitory
concentration
(IC-50)
values
LCOH
co-loaded
single
functionalized
liposome
(SFL)
(iRGDP-LiP,
TMAB-LiP)
dual-functionalized
(DFL)
(iRGDP-TMAB-LiP)
cells
1.04
±
0.023
μg/mL,
0.71
0.018
0.56
0.028
respectively.
Inverted
confocal
laser
scanning
microscopy
(ICLSM)
revealed
enhanced
cellular
internalization
SFL
DFL-treated
groups
tagged
coumarin-6
rhodamine-B
dye
compared
to
conventional
liposome.
scratch
assay
marked
reduction
migration,
DAPI
staining
confirmed
nuclear
condensation
(NC)
fragmentation
(NF)
groups.
Moreover,
flow
cytometry
demonstrated
early
late
apoptosis
DFL
These
findings
indicate
that
holds
promise
multifaceted
therapeutic
approach
Язык: Английский
Surface entrenched β-sitosterol niosomes for enhanced cardioprotective activity against isoproterenol induced cardiotoxicity in rats
International Journal of Pharmaceutics,
Год журнала:
2024,
Номер
653, С. 123872 - 123872
Опубликована: Фев. 8, 2024
Язык: Английский
αvβ3 Integrin and Folate-Targeted pH-Sensitive Liposomes with Dual Ligand Modification for Metastatic Breast Cancer Treatment
Bioengineering,
Год журнала:
2024,
Номер
11(8), С. 800 - 800
Опубликована: Авг. 7, 2024
The
advent
of
pH-sensitive
liposomes
(pHLips)
has
opened
new
opportunities
for
the
improved
and
targeted
delivery
antitumor
drugs
as
well
gene
therapeutics.
Comprising
fusogenic
dioleylphosphatidylethanolamine
(DOPE)
cholesteryl
hemisuccinate
(CHEMS),
these
nanosystems
harness
acidification
in
tumor
microenvironment
endosomes
to
deliver
effectively.
pH-responsive
that
are
internalized
through
endocytosis
encounter
mildly
acidic
pH
thereafter
fuse
or
destabilize
endosomal
membrane,
leading
subsequent
cargo
release
into
cytoplasm.
extracellular
matrix
also
presents
a
slightly
environment
can
lead
enhanced
drug
targeting
capabilities
nano-delivery
system.
Recent
studies
have
shown
folic
acid
(FA)
iRGD-coated
nanocarriers,
including
liposomes,
preferentially
accumulate
breast
tumors
overexpress
folate
receptors
αvβ3
αvβ5
integrins.
This
study
focuses
on
development
characterization
5-Fluorouracil
(5-FU)-loaded
FA
iRGD
surface-modified
pHLips
(FA-iRGD-5-FU-pHLips).
novelty
this
research
lies
dual
mechanism
utilizing
peptides,
combined
with
properties
enhance
selective
uptake
by
cancer
cells
effective
environment.
prepared
were
small,
an
average
diameter
152
±
3.27
nm,
uniform,
unilamellar,
demonstrating
efficient
5-FU
encapsulation
(93.1
2.58%).
Despite
surface
functionalization,
maintained
their
sensitivity
neutral
zeta
potential,
which
conferred
stability
reduced
aggregation.
Effective
responsiveness
was
demonstrated
observation
at
5.5
compared
physiological
7.4.
(84.47%
versus
46.41%
7.4,
respectively,
72
h).
formulations
exhibited
six
months
stable
when
subjected
simulated
biological
settings.
Blood
compatibility
cytotoxicity
MDA-MB-231
SK-BR3
cell
lines
revealed
liposomal
formulation
modified
free
minimal
hemolysis.
Collectively,
findings
support
potential
surface-camouflaged,
promising
strategy
treatment.
Язык: Английский
HER-2 Receptor and αvβ3 Integrin Dual-Ligand Surface-Functionalized Liposome for Metastatic Breast Cancer Therapy
Pharmaceutics,
Год журнала:
2024,
Номер
16(9), С. 1128 - 1128
Опубликована: Авг. 27, 2024
Human
epidermal
growth
factor
receptor-2
(HER2)-positive
breast
cancer
metastasis
remains
the
primary
cause
of
mortality
among
women
globally.
Targeted
therapies
have
revolutionized
treatment
efficacy,
with
Trastuzumab
(Trast),
a
monoclonal
antibody,
targeting
HER2-positive
advanced
cancer.
The
tumor-homing
peptide
iRGD
enhances
intratumoral
accumulation
and
penetration
therapeutic
agents.
Liposomes
serve
as
versatile
nanocarriers
for
both
hydrophilic
hydrophobic
drugs.
Gefitinib
(GFB)
is
potential
anticancer
drug
against
cancer,
while
Lycorine
hydrochloride
(LCH)
natural
compound
anti-inflammatory
properties.
This
study
developed
TPGS-COOH-coated
liposomes
co-loaded
GFB
LCH,
prepared
by
solvent
injection
method,
surface-functionalized
Trast
iRGD.
dual
surface-decorated
(DSDLs)
were
characterized
their
particle
size
(PS),
polydispersity
index
(PDI),
zeta
(ZP),
surface
chemistry,
morphology,
crystallinity
during
in-vitro
release,
encapsulation,
cell
line
studies
on
SK-BR-3
MDA-MB-231
cells.
half-maximum
inhibitory
concentration
(IC-50)
values
single
decorated
(SDLs),
iRGD-LP,
Trast-LP,
well
DSDLs
(iRGD-Trast-LP)
cells,
6.10
±
0.42,
4.98
0.36,
4.34
0.32
μg/mL,
respectively.
Moreover,
IC-50
SDLs
cells
15.12
0.68,
13.09
0.59,
11.08
0.48
Cellular
uptake
using
confocal
laser
scanning
microscopy
(CLSM)
showed
that
functionalization
significantly
enhanced
cellular
in
lines.
wound-healing
assay
demonstrated
significant
reduction
SDL
DSDL-treated
migration
compared
to
control.
Additionally,
blood
compatibility
minimal
hemolysis
(less
than
5%
RBC
lysis),
indicating
good
biocompatibility
biosafety.
Overall,
these
findings
suggest
TPGS-COOH-coated,
LCH
co-loaded,
dual-ligand
(iRGD
Trast)
functionalized,
multifunctional
could
be
promising
strategy
treating
metastatic
Язык: Английский
Nanoparticle-based flavonoid therapeutics: Pioneering biomedical applications in antioxidants, cancer treatment, cardiovascular health, neuroprotection, and cosmeceuticals
International Journal of Pharmaceutics,
Год журнала:
2024,
Номер
unknown, С. 125135 - 125135
Опубликована: Дек. 1, 2024
Язык: Английский
Synthesis and evaluation of valsartan co-crystals for enhanced solubility and anti-hypertensive activity
International Journal of Pharmaceutics,
Год журнала:
2025,
Номер
unknown, С. 125743 - 125743
Опубликована: Май 1, 2025
Язык: Английский
Protective activities of silver nanoparticles containing Panax japonicus on apoptotic, inflammatory, and oxidative alterations in isoproterenol-induced cardiotoxicity
Open Chemistry,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 1, 2024
Abstract
Panax
japonicus
has
long
been
utilized
as
an
herbal
remedy
in
Chinese
traditional
medicine
for
treating
various
diseases.
In
this
investigation,
we
present
the
environmentally
friendly
silver
nanoparticle
(AgNP)
synthesis
by
aqueous
extract
of
P.
japonicas
to
follow
its
cardioprotective
effects.
Through
analytical
methods,
identified
nanoparticles
(NPs).
Our
XRD
findings
revealed
formation
Ag@
,
while
FE-SEM
imagery
indicated
a
spherical
shape,
with
NPs
measuring
less
than
40
nm.
The
UV–Vis
and
FT-IR
spectroscopy
confirm
green
.
medicinal
section,
45
Wistar
rats
were
utilized.
These
groups
consisted
normal
group,
group
that
was
solely
treated
isoproterenol
inducing
myocardial
infarction,
two
pretreated
AgNPs
at
different
doses
14
days.
then
challenged
isoproterenol.
expression
PI3K/Akt/mTOR
other
downstream
inflammatory
apoptotic
mediators
followed.
Additionally,
Keap1,
Nrf2,
ECG,
cardiac
markers,
antioxidant
enzymes
assessed.
Treatment
ameliorated
apoptosis,
inflammation,
autophagy,
regulated
pathway,
increased
enzyme
efficacies,
activated
Keap1/Nrf2/HO-1
pathway.
suggest
may
have
efficacy
on
infarction
mitigating
Keap1/Nrf2
GST,
GPx,
GSH,
SOD,
IL-1β,
IL-6,
TNF-α,
NF-κB,
Bax,
Bcl2,
caspase-9,
caspase-3,
Furthermore,
treatment
decreased
infarct
region
size,
attenuated
indicators
levels,
mitigated
immune
cell
infiltration
necrosis.
Язык: Английский