Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158746 - 158746
Опубликована: Дек. 1, 2024
Язык: Английский
Journal of Biological Engineering, Год журнала: 2025, Номер 19(1)
Опубликована: Фев. 7, 2025
Язык: Английский
Процитировано
1
Biosensors, Год журнала: 2025, Номер 15(2), С. 80 - 80
Опубликована: Янв. 31, 2025
Detecting multiple tumor markers is of great importance. It helps in early cancer detection, accurate diagnosis, and monitoring treatment. In this work, gold nanoparticles–toluidine blue–graphene oxide (AuNPs-TB–GO) nanoparticles–carboxyl ferrocene–tungsten disulfide (AuNPs–FMC–WS2) nanocomposites were prepared for labeling Carcinoembryonic antigen (CEA) antibody Carbohydrate 72–4 (CA72-4) antibody, respectively, used as two kinds probes with different electrochemical signals. With the excellent magnetic performance biotin immune beads (IMBs), biofunctional IMBs firmly deposited on glassy carbon electrode (MGCE) surface by applying a constant field, then CEA CA72-4 immobilized avidin–biotin conjugation. The assay was based change detection peak current. Under optimum experimental conditions, linear range two-component immunosensor from 0.01 to 120 ng/mL, low limit 0.003 0.05 35 U/mL, 0.016 U/mL. results showed that proposed enabled simultaneous exhibited good reproducibility, high selectivity, sensitivity. particular, multiplexed immunoassay approach does not require sophisticated fabrication well-suited high-throughput biosensing application other areas.
Язык: Английский
Процитировано
0
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Апрель 18, 2025
DNA target-probe hybridization is a critical recognition and combination process for establishing high-performance biosensors. However, in conventional self-assembly strategies, surface-anchored capture probes exhibit heterogeneous molecular conformations limit the kinetics of at interface. As result, response speed practicability electrochemical biosensors are quite limited, especially real samples. Interfacial regulation conformation using artificial nanostructures has been widely recognized as promising strategy to improve accessibility activity probes. This study introduces significantly simplified regulatory structure on surface gold electrode consisting probe-polyA-probe (PAP) sequence probe (CP). The PAP central polyA fragment anchoring two flanking with ends probe, forming bridged CP (BCP). Upon dual-terminal PAP, underwent structural linearization through opposing directional extension, thereby markedly enhancing steric subsequent efficiency. By BCP biosensor, we achieved rapid sensitive detection from 1 fM nM. More importantly, platform demonstrated valuable versatility construction both gap biosensor microRNA DNAzyme Pb2+. exhibited exceptional biorecognition capability, achieving only 3 min remarkable efficiency 95.56%. Based its high sensitivity, operational simplicity, broad applicability, our shown an avenue development novel diagnostics environmental monitoring.
Язык: Английский
Процитировано
0
Journal of Materials Chemistry B, Год журнала: 2024, Номер 12(22), С. 5551 - 5560
Опубликована: Янв. 1, 2024
Fast and accurate identification of breast cancer utilizing sensitive electrochemical sensors gold columnar nanostructures.
Язык: Английский
Процитировано
2
Interdisciplinary cancer research, Год журнала: 2024, Номер unknown
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0
Опубликована: Янв. 1, 2024
Язык: Английский
Процитировано
0
Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 158746 - 158746
Опубликована: Дек. 1, 2024
Язык: Английский
Процитировано
0