Capture and detection of nucleic acid aptamer target proteins in colorectal cancer DOI

Huaqing Sun,

Hongyu Zhang, Liang Li

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158746 - 158746

Published: Dec. 1, 2024

Language: Английский

Nanozyme-based aptasensors for the detection of tumor biomarkers DOI Creative Commons
Ke Wang,

Xiaogang Lin,

Xiao Lv

et al.

Journal of Biological Engineering, Journal Year: 2025, Volume and Issue: 19(1)

Published: Feb. 7, 2025

Language: Английский

Citations

1
A Dual Nano-Signal Probe-Based Electrochemical Immunosensor for the Simultaneous Detection of Two Biomarkers in Gastric Cancer DOI Creative Commons

Li-Ting Su,

Zhenqing Yang, Hua‐Ping Peng

et al.

Biosensors, Journal Year: 2025, Volume and Issue: 15(2), P. 80 - 80

Published: Jan. 31, 2025

Detecting multiple tumor markers is of great importance. It helps in early cancer detection, accurate diagnosis, and monitoring treatment. In this work, gold nanoparticles–toluidine blue–graphene oxide (AuNPs-TB–GO) nanoparticles–carboxyl ferrocene–tungsten disulfide (AuNPs–FMC–WS2) nanocomposites were prepared for labeling Carcinoembryonic antigen (CEA) antibody Carbohydrate 72–4 (CA72-4) antibody, respectively, used as two kinds probes with different electrochemical signals. With the excellent magnetic performance biotin immune beads (IMBs), biofunctional IMBs firmly deposited on glassy carbon electrode (MGCE) surface by applying a constant field, then CEA CA72-4 immobilized avidin–biotin conjugation. The assay was based change detection peak current. Under optimum experimental conditions, linear range two-component immunosensor from 0.01 to 120 ng/mL, low limit 0.003 0.05 35 U/mL, 0.016 U/mL. results showed that proposed enabled simultaneous exhibited good reproducibility, high selectivity, sensitivity. particular, multiplexed immunoassay approach does not require sophisticated fabrication well-suited high-throughput biosensing application other areas.

Language: Английский

Citations

0
PolyA-Bridged Capture Probe Architecture Enables High-Efficiency DNA Hybridization for Multiplex Biosensing Applications DOI
Jiaqi Yang, Lele Wang,

Yanli Wen

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 18, 2025

DNA target-probe hybridization is a critical recognition and combination process for establishing high-performance biosensors. However, in conventional self-assembly strategies, surface-anchored capture probes exhibit heterogeneous molecular conformations limit the kinetics of at interface. As result, response speed practicability electrochemical biosensors are quite limited, especially real samples. Interfacial regulation conformation using artificial nanostructures has been widely recognized as promising strategy to improve accessibility activity probes. This study introduces significantly simplified regulatory structure on surface gold electrode consisting probe-polyA-probe (PAP) sequence probe (CP). The PAP central polyA fragment anchoring two flanking with ends probe, forming bridged CP (BCP). Upon dual-terminal PAP, underwent structural linearization through opposing directional extension, thereby markedly enhancing steric subsequent efficiency. By BCP biosensor, we achieved rapid sensitive detection from 1 fM nM. More importantly, platform demonstrated valuable versatility construction both gap biosensor microRNA DNAzyme Pb2+. exhibited exceptional biorecognition capability, achieving only 3 min remarkable efficiency 95.56%. Based its high sensitivity, operational simplicity, broad applicability, our shown an avenue development novel diagnostics environmental monitoring.

Language: Английский

Citations

0
Precision in cancer diagnostics: ultra-sensitive detection of MCF-7 breast cancer cells by gold nanostructure-enhanced electrochemical biosensing DOI

Mahsa Rahmanipour,

Hossein Siampour, Ahmad Moshaii

et al.

Journal of Materials Chemistry B, Journal Year: 2024, Volume and Issue: 12(22), P. 5551 - 5560

Published: Jan. 1, 2024

Fast and accurate identification of breast cancer utilizing sensitive electrochemical sensors gold columnar nanostructures.

Language: Английский

Citations

2
Nanotechnology for Cancer Research (Diagnosis and Therapy): Recent Progress and Future Prospects DOI
Wessam Wanas,

Dalia Nabil Khalifa,

H Gamal

et al.

Interdisciplinary cancer research, Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 1, 2024

Language: Английский

Citations

0
Capture And Detection of Nucleic Acid Aptamer Target Proteins in Colorectal Cancer DOI
Hong Sun, Hongyu Zhang, Liang Li

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Capture and detection of nucleic acid aptamer target proteins in colorectal cancer DOI

Huaqing Sun,

Hongyu Zhang, Liang Li

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158746 - 158746

Published: Dec. 1, 2024

Language: Английский

Citations

0