Biosensors,
Год журнала:
2024,
Номер
14(12), С. 633 - 633
Опубликована: Дек. 20, 2024
Antibiotics,
celebrated
as
some
of
the
most
significant
pharmaceutical
breakthroughs
in
medical
history,
are
capable
eliminating
or
inhibiting
bacterial
growth,
offering
a
primary
defense
against
wide
array
infections.
However,
rise
antimicrobial
resistance
(AMR),
driven
by
widespread
use
antibiotics,
has
evolved
into
and
ominous
threat
to
global
public
health.
Thus,
creation
efficient
methods
for
detecting
genes
antibiotics
is
imperative
ensuring
food
safety
safeguarding
human
The
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
CRISPR-associated
proteins
(Cas)
systems,
initially
recognized
an
adaptive
immune
mechanism
bacteria
archaea,
have
unveiled
their
profound
potential
sensor
detection,
transcending
notable
gene-editing
applications.
CRISPR/Cas
technology
employs
Cas
enzymes
guides
RNA
selectively
target
cleave
specific
DNA
sequences.
This
review
offers
extensive
examination
highlighting
unique
attributes
applications
antibiotic
detection.
It
outlines
current
utilization
progress
toolkit
identifying
both
nucleic
acid
(resistance
genes)
non-nucleic
(antibiotic
micromolecules)
targets
within
field
In
addition,
it
examines
challenges,
such
sensitivity
specificity,
future
opportunities,
including
development
point-of-care
diagnostics,
providing
strategic
insights
facilitate
curbing
oversight
antibiotic-resistance
proliferation.
Environment International,
Год журнала:
2024,
Номер
191, С. 108972 - 108972
Опубликована: Авг. 22, 2024
Overutilization
and
misuse
of
antibiotics
in
recent
decades
markedly
intensified
the
rapid
proliferation
diffusion
antibiotic
resistance
genes
(ARGs)
within
environment,
thereby
elevating
ARGs
to
status
a
global
public
health
crisis.
Recognizing
that
soil
acts
as
critical
reservoir
for
ARGs,
environmental
researchers
have
made
great
progress
exploring
sources,
distribution,
spread
soil.
However,
microscopic
state
micro-interfacial
behavior
remains
inadequately
understood.
In
this
study,
we
reviewed
behaviors
antibiotic-resistant
bacteria
(ARB)
porous
media,
predominantly
including
migration-deposition,
adsorption,
biofilm
formation.
Meanwhile,
proliferation,
degradation
were
identified
primary
soil,
with
component
serving
significant
determinant.
Our
work
contributes
further
comprehension
microstates
processes
ARB
environments
offers
theoretical
foundation
managing
mitigating
risks
associated
ARG
contamination.
Materials Research Express,
Год журнала:
2024,
Номер
11(5), С. 055601 - 055601
Опубликована: Май 1, 2024
Abstract
TiO
2
/
g
-C
3
N
4
/biochar
(TCNBC)
catalysts
were
prepared
by
the
hydrolysis
method
for
photocatalytic
degradation
of
doxycycline
antibiotic
(DC),
with
biochar
obtained
from
pyrolysis
Phragmites
australis.
The
examined
using
scanning
electron
microscope
(SEM),
transmission
microscopy
(TEM),
energy
dispersive
x-ray
spectrometer
(EDX),
photoelectron
spectroscopy
(XPS),
diffraction
(XRD),
Fourier-transform
infrared
(FTIR),
photoluminescence
(PL),
and
ultraviolet-visible
diffuse
reflectance
(UV–Vis
DRS)
nitrogen
adsorption/desorption.
activity
results
showed
that
TCNBC
catalyst
exhibited
higher
catalytic
than
pure
or
.
Its
peak
activity,
achieving
a
decomposition
efficiency
91.93%
mineralization
81.50%,
can
be
attributed
to
synergistic
effect
biochar,
,
Even
after
four
cycles
use,
still
maintained
relatively
high
DC.
decreased
86.30%
recycling
events.
Environmental Science and Pollution Research,
Год журнала:
2024,
Номер
31(36), С. 48813 - 48838
Опубликована: Июль 25, 2024
The
increase
in
the
prevalence
of
carbapenem-resistant
Gram-negative
bacteria,
particular
Acinetobacter
baumannii
(CRAB)
and
Pseudomonas
aeruginosa
(CRPA),
poses
a
serious
threat
for
public
health
worldwide.
This
article
reviews
alarming
data
on
infections
caused
by
CRAB
CRPA
pathogens
their
presence
hospital
municipal
wastewater,
it
highlights
environmental
impact
antibiotic
resistance.
describes
key
role
resistance
genes
(ARGs)
acquisition
carbapenem
sheds
light
bacterial
mechanisms.
main
emphasis
was
placed
transfer
ARGs
not
only
clinical
setting,
but
also
environment,
including
water,
soil,
food.
aim
this
review
to
expand
our
understanding
global
risks
associated
with
wastewater
analyze
spread
these
micropollutants
environment.
A
literature
published
last
decade
will
direct
research
pathogens,
support
implementation
effective
preventive
measures
interventions,
contribute
development
improved
strategies
managing
problem.
Agronomy,
Год журнала:
2025,
Номер
15(2), С. 397 - 397
Опубликована: Фев. 1, 2025
The
use
of
dairy
slurries
as
organic
fertilizer
amendments
is
a
common
practice
in
agriculture
cost-saving
measure,
well
residue
management
strategy.
However,
concerns
related
to
the
increase
antibiotic
resistance
environment
under
scope
One
Health
strategy
are
increasing.
In
this
study,
we
aimed
assess
resistome
enrichment
driven
by
slurry
application
four
southern
Chile
farms.
Slurry
pits,
rhizospheres
Lolium
perenne
amended
with
those
slurries,
and
bulk
soils
were
sampled.
Thirteen
antibiotic-resistance
genes
(ARGs,
tetA,
tetG,
tetM,
tetQ,
tetW,
tetX,
sul1,
sul2,
blaCTXM,
blaOXA-1,
blaTEM,
ermB,
dfrA1)
for
five
classes
(tetracyclines,
sulfonamides,
beta-lactams,
macrolides,
trimethoprim–sulfamethoxazole),
two
integrases
(intl1
intl2),
total
bacteria
(16S
rRNA)
abundance
was
measured
quantitative
PCR
(qPCR).
Then,
profiles
enzyme-inactivated
ARGs
(tetX
blaTEM)
determined.
differences
between
bacterial
communities
inhabiting
different
sample
types
explored
16S
rRNA
metabarcoding.
general,
all
detected
slurries.
A
decreasing
trend
ARG
copy
numbers
observed
increasing
soil
depth,
exception
whose
increased
at
specific
tetX
blaTEM
revealed
no
relative
variants
any
samples.
Finally,
taxonomic
structural
found
among
types.
Thus,
sampled
farm
resistomes
raw
fertilizer.
Khwaja Yunus Ali University journal.,
Год журнала:
2025,
Номер
unknown, С. 01 - 09
Опубликована: Март 26, 2025
Antimicrobial
resistance
(AMR)
is
a
growing
global
health
concern,
exacerbated
by
the
overuse
of
antibiotics
in
animal
husbandry,
particularly
poultry
industry.
A
cross-sectional
study
was
conducted
between
July
2021
and
2022,
collecting
80
samples
from
farms
Savar,
Hemayetpur,
Manikganj,
Gazipur,
Tangail.
total
106
bacterial
isolates
were
identified,
where
Escherichia
coli
became
most
prevalent
(47.2%),
followed
Enterobacter
cloacae
(12.3%)
Citrobacter
freundii
(5.7%).
susceptibility
testing
revealed
high
to
beta-lactam
antibiotics,
including
carbapenems,
with
73.6%
resistant
imipenem
61.3%
amoxicillin-clavulanic
acid.
The
blaTEM
gene
detected
33%
isolates.
weak
association
phenotypic
presence
observed.
prevalence
multidrug-resistant
bacteria
ESBL
genes
feces
indicates
significant
risk
transmission
humans,
posing
threat
public
health.
This
aimed
investigate
one
prominent
Extended
Spectrum
Beta-Lactamase
(ESBL)
gene,
Bangladesh.
Environmental Science and Pollution Research,
Год журнала:
2024,
Номер
31(35), С. 47727 - 47741
Опубликована: Июль 15, 2024
The
study
describes
the
whole-genome
sequencing
of
two
antibiotic-resistant
representative
Escherichia
coli
strains,
isolated
from
poultry
manure
in
2020.
samples
were
obtained
a
commercial
chicken
meat
production
facility
Poland.
antibiotic
resistance
profile
was
characterized
by
co-resistance
to
β-lactam
antibiotics,
aminoglycosides,
and
fluoroquinolones.
three
identified
plasmids
(R-plasmids),
pECmdr13.2,
pECmdr13.3,
pECmdr14.1,
harbored
various
genes
conferring
tetracyclines
(tetR[A])
for,
aminoglycoside
(aph,
aac,
aad
families),
(bla
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Май 20, 2024
Abstract
The
study
describes
the
whole-genome
sequencing
of
two
antibiotic-resistant
representative
Escherichia
coli
strains,
isolated
from
poultry
manure
in
2020.
samples
were
obtained
a
commercial
chicken
meat
production
facility
Poland.
antibiotic
resistance
profile
was
characterized
by
co-resistance
to
β-lactam
antibiotics,
aminoglycosides,
and
fluoroquinolones.
three
identified
plasmids
(R-plasmids),
pECmdr13.2,
pECmdr13.3
pECmdr14.1,
harbored
various
genes
conferring
tetracyclines
(tetR[A])
for,
aminoglycoside
(aph,
aacaad
families),
(blaCMY-2,
blaTEM-176),
sulfonamide
(sul1,
sul2),
fluoroquinolone
(qnrS1),
phenicol
(floR).
These
plasmids,
which
have
not
been
previously
reported
Poland,
found
carry
IS26
insertion
elements,
intI1-integrase
gene,
as
well
conjugal
transfer
genes,
facilitating
horizontal
gene
transfer.
Plasmids
pECmdr13.2
pECmdr14.1
also
possessed
mercury
operon
related
transposon
Tn1696;
this
promotes
plasmid
persistence
even
without
selection
pressure
due
co-selection
mechanisms
such
co-resistance.
manure-derived
belonged
IncX1
(narrow
host
range)
IncC
(broad
incompatibility
groups.
Similar
environments,
clinical
isolates,
farm
animals,
including
cattle,
swine,
poultry.
This
holds
significant
importance
for
One
Health
approach,
it
highlights
potential
antimicrobial-resistant
bacteria
livestock
food
sources,
particularly
E.
coli,
through
chain
humans
vice
versa.
underscores
need
vigilant
monitoring
R-plasmids
prevalence
human,
animal
natural
implement
strategies
mitigate
spread
resistance.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 5, 2024
Abstract
The
study
describes
the
whole-genome
sequencing
of
two
antibiotic-resistant
representative
Escherichia
coli
strains,
isolated
from
poultry
manure
in
2020.
samples
were
obtained
a
commercial
chicken
meat
production
facility
Poland.
antibiotic
resistance
profile
was
characterized
by
co-resistance
to
β-lactam
antibiotics,
aminoglycosides,
and
fluoroquinolones.
three
identified
plasmids
(R-plasmids),
pECmdr13.2,
pECmdr13.3
pECmdr14.1,
harbored
various
genes
conferring
tetracyclines
(
tetR[A]
)
for,
aminoglycoside
aph,
aac
aad
families),
bla
CMY-2
,
TEM-176
),
sulfonamide
sul1,
sul2
fluoroquinolone
qnrS1
phenicol
floR
).
These
plasmids,
which
have
not
been
previously
reported
Poland,
found
carry
IS26
insertion
elements,
intI1-integrase
gene,
as
well
conjugal
transfer
genes,
facilitating
horizontal
gene
transfer.
Plasmids
pECmdr13.2
pECmdr14.1
also
possessed
mercury
operon
related
transposon
Tn1696;
this
promotes
plasmid
persistence
even
without
selection
pressure
due
co-selection
mechanisms
such
co-resistance.
manure-derived
belonged
IncX1
(narrow
host
range)
IncC
(broad
incompatibility
groups.
Similar
environments,
clinical
isolates,
farm
animals,
including
cattle,
swine,
poultry.
This
holds
significant
importance
for
One
Health
approach,
it
highlights
potential
antimicrobial-resistant
bacteria
livestock
food
sources,
particularly
E.
through
chain
humans
vice
versa.
underscores
need
vigilant
monitoring
R-plasmids
prevalence
human,
animal
natural
implement
strategies
mitigate
spread
resistance.
