Jujing
Zhuyu
decoction
(JZD)
is
a
traditional
Chinese
medicine
that
effectively
improves
sperm
motility.
However,
the
molecular
mechanism
of
JZD
on
asthenozoospermia
still
remains
unknown.
In
this
study,
we
investigated
effect
mitochondrial
apoptosis
pathway
in
an
rat
model.
Sixty
Sprague-Dawley
rats
were
randomly
divided
into
five
groups-control,
tripterygium
glycosides
(GTW)
model,
JZD-low
(JZD-L),
JZD-medium
(JZD-M),
and
JZD-high
(JZD-H)
groups
(n
=
12/group).
GTW
was
used
to
generate
The
JZD-L,
JZD-M,
JZD-H
administered
5,
10,
or
15
g
kg-1
day-1
granules
respectively,
for
4
weeks.
Testicular
tissue
morphology
examined
using
histological
staining,
while
count
determined
manual
computer-aided
semen
analyses.
Apoptosis
spermatogenic
cells
detected
with
TUNEL
assay,
expression
proteins
genes
related
western
blotting
quantitative
reverse
transcription-polymerase
chain
reaction
respectively.
Histomorphological
evaluation
revealed
superior
seminiferous
tubule
structure
arrangement
as
well
improved
cell
compared
those
model
group.
Moreover,
quality
apoptotic
index
significantly
Additionally,
mRNA
protein
abundance
Apaf-1,
Bax,
Cyto-c,
caspase-3
reduced,
Bcl-2
increased
all
reduces
rate
promotes
survival
by
regulating
pathway.
This
provides
experimental
support
treatment
JZD.
Plants,
Год журнала:
2024,
Номер
13(11), С. 1531 - 1531
Опубликована: Май 31, 2024
Natural
plants
contain
numerous
chemical
compounds
that
are
beneficial
to
human
health.
The
berries
from
the
Lycium
genus
widely
consumed
and
highly
nutritious.
Moreover,
their
constituents
have
attracted
attention
for
health-promoting
properties.
In
East
Asia,
there
three
varieties
of
(
Frontiers in Pharmacology,
Год журнала:
2023,
Номер
14
Опубликована: Фев. 17, 2023
Background:
We
determined
the
effects
of
Cuscutae
semen
(Cuscuta
chinensis
Lam.
or
Cuscuta
australis
R.
Br.)-Radix
rehmanniae
praeparata
(Rehjnannia
glutinosa
Libosch.)
on
protein
levels
in
testicular
tissues
rats
gavaged
with
tripterygium
wilfordii
multiglycosides
(GTW)
and
elucidated
molecular
mechanism
underlying
semen-Radix
for
relieving
GTW-induced
reproductive
injury.
Methods:
A
total
21
male
Sprague-Dawley
were
randomly
divided
into
control
group,
model
group
based
their
body
weights.
The
was
given
10
mLkg-1
0.9%
normal
saline
by
gavage
daily.
(GTW
group)
administered
12
mg
kg-1
GTW
(the
TSZSDH
1.56
gkg-1
granules
daily
according
to
dosing.
serum
luteinizing
hormone,
follicle-stimulating
estradiol,
testosterone
measured
after
weeks
continuous
gavage,
pathological
lesion
observed.
Differentially
expressed
proteins
evaluated
quantitative
proteomics
verified
western
blotting
(WB)
Real-Time
Quantitative
Polymerase
Chain
Reaction
(RT-qPCR).
Results:
can
effectively
relieve
lesions
tissues.
216
differentially
identified
group.
High-throughput
revealed
that
are
closely
associated
peroxisome
proliferator-activated
receptor
(PPAR)
signaling
pathway,
digestion
absorption,
glycan
pathway
cancer.
significantly
upregulate
expressions
Acsl1,
Plin1,
Dbil5,
Plin4,
Col12a1,
Col1a1,
Col5a3,
Col1a2,
Dcn,
so
as
play
a
protective
role
PPARγ
PPAR
WB
RT-qPCR
experiments,
which
found
be
consistent
results
analysis.
Conclusion:
Radix
may
regulate
mediated
Plin1
reduce
tissue
damage
caused
GTW.
Journal of Ethnopharmacology,
Год журнала:
2021,
Номер
280, С. 114440 - 114440
Опубликована: Июль 19, 2021
Wu-Zi-Yan-Zong-Wan
(WZYZW)
is
a
classical
traditonal
Chinese
herbal
formula
and
patent
medicine
used
to
treat
male
infertility.
However,
the
chemical
components
of
WZYZW
its
mechanism
are
not
yet
fully
clarified.The
purpose
this
study
observe
effect
underlying
on
ameliorating
blood-testis
barrier
(BTB)
dysfunction
in
mice
with
spermatogenic
induced
by
administration
Tripterygium
wilfordii
Hook.
f.
multiglycosides
(GTW).WZYZW
was
administered
gavage
GTW-induced
(kidney
essence
deficiency
pattern)
for
40
days.
Testis
tissues
were
obtained
subsequent
histopathological
analysis.
Biotin
tracing
evaluate
permeability
Sertoli
cell
tight
junctions.
The
levels
proinflammatory
cytokines
including
interleukin
(IL)-6,
IL-17A,
IL-1α
tumor
necrosis
factor
(TNF)-α
analyzed
ELISA.
expression
proteins
related
junction
ZO-1,
JAM-A
occludin
western
blotting.
ultrastructures
junctions
observed
transmission
electron
microscopy.WZYZW
ameliorated
testicular
dysfunction.
Levels
IL-6,
IL-1α,
TNF-α
groups
receiving
low,
medium,
high
doses
decreased
dose-dependent
manner.
impeded
biotin
tracer
from
permeating
BTB,
protecting
integrity
GTW-treated
mice.
In
addition,
our
results
showed
no
significant
changes
protein
expressions
JAM-A,
after
compared
GTW
group.
Meanwhile,
exhibited
linear
arrangement
restored
typical
"sandwich"
structure
BTB.
No
acute
poisoning
incidences
all
during
experiment.Our
findings
demonstrate
that
may
ameliorate
some
BTB
dysfunction,
possibly
regulating
cytokine
levels.
vitro
studies
regulation
active
further
required.
Evidence-based Complementary and Alternative Medicine,
Год журнала:
2022,
Номер
2022, С. 1 - 11
Опубликована: Июнь 18, 2022
Idiopathic
oligoasthenozoospermia
(iOAZS)
is
one
of
the
major
causes
male
infertility,
and
ideal
therapies
for
iOAZS
have
not
been
established
yet.
Traditional
Chinese
medicine
(TCM),
including
Xianlu
oral
solution
(XL),
has
widely
used
as
an
adjunct
treatment
infertility
in
clinic.
However,
underlying
mechanisms
XL
on
are
still
known.
Here,
we
found
that
therapeutic
effects
ornidazole
(ORN)-induced
OAZS
model
rats
through
amelioration
testis
tissues
spermatogenesis
improvement
sperm
concentration
motility.
Moreover,
ameliorated
serum
hormone
levels,
mitochondrial
membrane
potential,
apoptosis
status,
oxidative
stress
status
rats.
These
findings
identify
a
potential
mechanism
iOAZS,
may
be
traditional
(TCM)
therapy
caused
by
clinical
practice.
Pharmaceutical Biology,
Год журнала:
2023,
Номер
61(1), С. 674 - 682
Опубликована: Апрель 25, 2023
Context
Zhibai
Dihuang
pill
(ZD),
a
traditional
Chinese
medicine
nourishes
Yin
and
reduces
internal
heat,
is
believed
to
have
therapeutic
effects
on
urinary
tract
infections
(UTIs).Objective
To
explore
the
mechanism
of
modified
ZD
(MZD)
UTI
induced
by
extended-spectrum
β-lactamase
(ESBLs)
Escherichia
coli.Materials
methods
Thirty
Sprague-Dawley
rats
were
randomly
divided
into
control,
model
(0.5
mL
1.5
×
108
CFU/mL
ESBLs
E.
coli),
MZD
(20
g/kg
MZD),
LVFX
(0.025
LVFX),
+
groups
0.025
n
=
6.
After
14
days
treatment,
serum
biochemical
indicators,
renal
function
bladder
histopathology,
urine
bacterial
counts
in
determined.
Additionally,
coli
biofilm
formation
related
gene
expression
analyzed.Results
significantly
decreased
count
white
blood
cells
(from
13.12
9.13),
proportion
neutrophils
43.53
23.18),
C-reactive
protein
13.21
9.71),
creatinine
35.78
30.15),
urea
nitrogen
12.56
10.15),
relieved
inflammation
fibrosis
kidney
tissues,
reduced
number
bacteria
2174
559).
In
addition,
inhibited
biofilms
(2.04-fold)
expressions
luxS,
pfS
ompA
(1.41–1.62-fold).Discussion
conclusion
treated
coli-induced
formation,
providing
theoretical
basis
for
clinical
application
MZD.
Further
study
effect
may
provide
novel
therapy
option
UTI.
Zuogui
Wan
(ZGW)
is
a
common
prescription
medication
used
in
traditional
Chinese
medicine
(TCM)
to
significantly
improve
the
sperm
quality
and
treat
male
infertility.
This
study
evaluated
repair
effect
of
ZGW
Levocarnitine
(LEV)
on
GC1-spg
cell
injury
induced
by
Glucosides
Tripterygium
WilforDII
Hook
(GTW).
The
results
showed
that
ultrastructure
apoptosis
rate
GC1-
spg
cells
LEV
group
were
considerably
better
than
GTW.
transcriptional
translational
level
CYP1A1,
CYP17A1,
androgen
receptor
(AR),
SRD5A2
proliferating
nuclear
antigen
(PCNA)
GC-1spg
elevated
GTW
(p
<
0.05
or
0.01).
Furthermore,
levels
CYP19A1,
AR,
PCNA
found
be
findings
indicate
could
increase
expression
PCNA,
AR
at
levels,
inhibit
promoting
proliferation,
was
LEV.
Pharmacological Research - Modern Chinese Medicine,
Год журнала:
2022,
Номер
4, С. 100129 - 100129
Опубликована: Июнь 16, 2022
Male
infertility
(MI)
is
a
multifactorial
disease
leading
to
the
disorder
of
male
reproductive
dysfunctions,
which
affecting
approximately
50%
partners
in
infertile
couples.
has
become
third
most
difficult
world,
since
cardiovascular
and
cancer.
Although
conventional
treatments
such
as
medication,
surgical
operation,
advanced
techniques
have
helped
many
with
cause
pregnancy
their
female
partners,
effectiveness
not
satisfactory
associated
adverse
effects.
Traditional
Chinese
Medicine
(TCM)
been
taken
for
clinical
application
improve
thousands
years
China.
The
pharmacological
mechanism
TCM
also
widely
studied.
In
this
study,
we
will
summarize
effective
treating
MI,
evaluations
molecular
mechanisms
Additionally,
point
out
limitations
current
research
challenges
faced
by
modernization
TCM.
Journal of Traditional Chinese Medical Sciences,
Год журнала:
2024,
Номер
11(2), С. 180 - 190
Опубликована: Март 13, 2024
To
investigate
the
mechanism
of
action
Wuzi
Yanzong
pill
(WYP)
in
rats
with
oligoasthenozoospermia
(OAZ)
via
metabolomics
and
to
provide
a
possible
basis
for
improving
this
WYP-based
treatment.
A
rat
model
OAZ
was
established
by
treating
male
Sprague-Dawley
glucosides
from
Tripterygium
wilfordii
Hook.
F.
Seventy-two
were
randomly
divided
into
six
groups:
control,
L-carnitine
(positive
control),
model,
low-,
medium-,
high-dose
WYP
groups.
Rats
experimental
groups
treated
4
weeks.
At
end
treatment
period,
sperm
cell
quality
(density,
motility,
viability)
assessed
using
semen
analysis
system,
mitochondrial
membrane
potential
(MMP)
flow
cytometry,
testicular
injury
hematoxylin
eosin
staining
validate
therapeutic
effect
OAZ.
Further,
serum
metabolomics-based
performed
high-performance
liquid
chromatography-mass
spectrometry
identify
differential
metabolic
pathways
mechanisms
considered
successfully-established
after
comparing
spermatozoa
group
that
control
group.
WYP-M
WYP-H
treatments
significantly
improved
density,
viability
compared
those
(all
P
<
.05).
Compared
group,
both
increased
MMP
values
(P
=
.006
.021
respectively),
while
there
no
significant
difference
administration
reversed
damage
testes
varying
degrees
44
metabolites
four
pathways,
especially
autophagy
pathway,
related
identified
metabolomics.
improves
primarily
regulation.
These
findings
can
be
employed
improve
efficacy
humans.
