Drug Design Development and Therapy,
Год журнала:
2024,
Номер
Volume 18, С. 5541 - 5561
Опубликована: Дек. 1, 2024
Background:
DaYuan
Yin
(DYY),
a
traditional
Chinese
medicine
for
lung
diseases,
requires
further
study
to
understand
how
it
improves
acute
injury
(ALI).
This
seeks
elucidate
the
material
basis
and
molecular
mechanisms
underlying
treatment
of
ALI
with
DYY
through
network
pharmacology,
docking,
experimental
validation.
Methods:
DYY's
active
components
targets
were
identified
using
TCMSP
UHPLC-MS/MS,
herb-component-target
was
created
Cytoscape
3.7.2.
target
genes
sourced
from
GeneCards,
DisGeNET,
DrugBank.
A
PPI
built,
core
analyzed
GO
KEGG
enrichment
via
Metscape.
The
therapeutic
effects
on
LPS-induced
in
rats
explored,
docking
evaluated
interactions
between
Nrf2,
HO-1,
TLR4,
components.
Results:
95
compounds,
234
targets,
2529
ALI-related
genes,
111
shared
ALI.
analysis
indicates
that
PI3K-AKT,
MAPK,
oxidative
stress
pathways
are
associated
anti-ALI
effects.
Network
pharmacology
UHPLC-MS/MS
revealed
ingredients
like
quercetin,
Magnolol,
Wogonin.
Compared
model
group,
reduced
dry–wet
ratio
(W/D)
(5.31
±
0.51)
(4.47
0.73)(
P
<
0.05).
Meanwhile,
contents
IL-6
TNF-α
bronchoalveolar
lavage
fluid
(BALF)
MDA,
NO
ROS
tissue
also
significantly
decreased.
Notably,
enhances
UCP2
mRNA
expression,
boosts
Nrf2
HO-1
inhibits
TLR4-mediated
pro-inflammatory
mediators.
Molecular
showed
main
had
strong
binding
ability
HO-1.
Conclusion:
can
alleviate
inflammation,
stress,
changes
by
targeting
Nrf2/HO-1
mediated
TLR4
pathway,
providing
insights
developing
effective
treatments.
Keywords:
Yin,
Acute
injury,
Oxidative
Anti-inflammatory,
Phytomedicine,
Год журнала:
2024,
Номер
132, С. 155817 - 155817
Опубликована: Июнь 12, 2024
Brassaiopsis
glomerulata
(Blum)
Regel
(B.glomerulata)
is
recognized
as
a
traditional
Chinese
medicine
(TCM)
primarily
used
for
promoting
blood
circulation
and
removing
stasis.
It
frequently
utilized
in
the
treatment
of
injuries
resulting
from
falls
bumps.
Molecular Medicine,
Год журнала:
2025,
Номер
31(1)
Опубликована: Март 7, 2025
Abstract
Background
Canopy
FGF
signaling
regulator
3
(CNPY3)
has
been
implicated
in
tumor
progression.
However,
its
specific
role
colon
cancer
(CC)
remains
unclear.
This
study
aims
to
investigate
the
function
of
CNPY3
CC
and
potential
as
a
therapeutic
target.
Methods
A
total
201
tissue
specimens
67
adjacent
non-cancerous
tissues
were
collected
for
analysis.
expression
was
assessed
using
immunohistochemistry
quantitative
real-time
PCR.
Functional
assays
conducted
cell
lines
(HT-29
SW-620)
following
knockdown
evaluate
effects
on
proliferation,
migration,
apoptosis.
Gene
profiling,
fibroblast
co-culture
experiments,
vivo
xenograft
models
also
conducted.
Results
Increased
correlated
with
advanced
stages
poorer
prognosis.
Knockdown
significantly
inhibited
induced
apoptosis
lines.
depletion
modulated
behavior,
inhibiting
their
transformation
into
cancer-associated
fibroblasts.
Pathway
analysis
revealed
that
affected
cycle
p53
pathways,
reduced
activation
MAPK
PI3K/AKT
pathways.
Additionally,
enhanced
sensitivity
5-fluorouracil.
In
studies
demonstrated
resulted
smaller
sizes
weights
than
controls.
Conclusions
is
crucial
progression,
correlating
aggressiveness
poor
patient
outcomes.
Targeting
may
offer
promising
strategy
valuable
prognostic
marker
management.
BMC Medical Genomics,
Год журнала:
2025,
Номер
18(1)
Опубликована: Март 19, 2025
To
investigate
the
role
of
Biglycan(BGN)
in
progression
and
metastasis
clear
cell
renal
carcinoma(ccRCC).
Based
on
multiple
public
databases,
we
investigated
expression
level
BGN
ccRCC,
its
clinical
significance,
association
with
immune
cells.
Real-time
fluorescence
quantitative
polymerase
chain
reaction(PCR)
was
employed
to
validate
tumor
adjacent
normal
tissues
from
ten
patients.
We
utilized
RNA
sequencing
results
for
further
analysis,
including
differential
gene
GO-KEGG
GSEA
identify
signaling
pathways
through
which
exerts
effects.
knockdown
cells(786-0
Caki-1)
were
generated
lentiviral
transfection
examine
impact
ccRCC.
Cell
proliferation,
migration,
invasion
assessed
using
CCK8,
colony
formation,
wound
healing,
Transwell
assays,
respectively.
Our
findings
database
analysis
PCR
revealed
a
significant
upregulation
kidney
cancer
compared
tissues.
Further
demonstrated
correlation
between
high
ccRCC
infiltration.
In
vitro
experiments
confirmed
that
silencing
effectively
inhibited
Mechanistically,
these
effects
may
be
mediated
MAPK
pathway.
potentially
plays
pivotal
possibly
acting
Therefore,
holds
promise
as
potential
therapeutic
target
Rapid Communications in Mass Spectrometry,
Год журнала:
2025,
Номер
39(12)
Опубликована: Март 25, 2025
The
ingredients
of
traditional
Chinese
medicine
(TCM)
are
complex
and
diverse
often
identified
using
the
TCM
systems
pharmacology
(TCMSP)
database.
However,
in
this
database
exhibit
significant
homogeneity,
which
limits
comprehensive
understanding
TCM's
ingredient
diversity
biological
activity.
Consequently,
liquid
chromatography-mass
spectrometry
(LC-MS)
has
become
a
key
tool
for
precise
identification
components.
In
study,
LC-MS
was
used
to
identify
components
herb.
were
then
compared
with
component
data
TCMSP
A
network
pharmacological
prediction
subsequently
performed.
An
analysis
four
herb
formula
prescriptions
(Ganmai
Dazao
decoction,
Bazhen
granule,
Shaoyao
Gancao
Zixue
san)
conducted,
revealing
discrepancies
between
results
found
be
highly
homogeneous.
This
study
underscores
pivotal
function
TCM,
particularly
addressing
issues
homogeneity.
employment
technology
enhances
precision
streamlines
potential
pharmacodynamic
ingredients,
thereby
propelling
toward
more
contemporary
standing.
