Network Pharmacology and Molecular Analysis of Mechanisms underlying the therapeutic effects of Rhubarb in treating Atherosclerosis and Abdominal Aortic Aneurysm

Heliyon, Год журнала: 2025, Номер 11(4), С. e41906 - e41906

Опубликована: Янв. 23, 2025

The aim of this study was to systematically investigate the effects and mechanisms Rhubarb in treatment Atherosclerosis (AS) Abdominal Aortic Aneurysm (AAA) by utilizing network pharmacology molecular docking techniques. TCMSP systematic database utilized search for active chemical components Rhubarb. Disease-related targets were retrieved from GEO dataset Disgenet database. Gene interactions identify common with AS/AAA, interaction networks constructed using Cytoscape 3.9.1. Protein-protein (PPI) core STRING GO KEGG pathway enrichment analysis performed DAVID. Molecular is used assess potential target-active compound interactions. In our study, 16 compounds screened Rhubarb, along 310 targets. Additionally, 110 AS/AAA target genes out. Topological PPI protein yielded 23 targets, biological functions signaling pathways further investigated. indicated that may be effective treating through processes such as lipids, atherosclerosis, extracellular matrix catabolism, collagenolytic metabolic processes, environment. Five pharmacological also identified: TNF, IL-1β MMP9, TP53, PPARG. showed a strong binding ability between This successfully predicted functions, associated AS/AAA. addition, identified ingredients can source drug screening.

Язык: Английский

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Study on the correlation between circadian rhythm, intestinal flora and Liver stiffness in patients with Hepatic fibrosis

Research Square (Research Square), Год журнала: 2025, Номер unknown

Опубликована: Март 17, 2025

Background: Hepatic fibrosis (HF) is a critical pathological process in chronic liver diseases, and its progression closely associated with gut microbiota dysbiosis circadian rhythm disruption. However, the interplay between these factors HF remains poorly understood. This study aimed to investigate relationship composition, disturbances, HF, providing new insights into potential therapeutic strategies. Methods: A cross-sectional was conducted, enrolling patients healthy controls. Liver stiffness measurement (LSM) assessed using transient elastography. Circadian status evaluated Morningness-Eveningness Questionnaire-5 (MEQ-5). Gut composition analyzed via 16S rRNA sequencing, differences microbial diversity taxa abundance were compared groups. Correlation analyses performed explore associations microbiota, LSM, rhythm. Results: Patients exhibited significant alterations at both phylum genus levels (p<0.05, r = − 0.244). The relative abundances of Escherichia-Shigella, Klebsiella, Pseudomonadota, Ruminococcus gnavus group, Enterocloster significantly increased, while Dorea, Holdemanella, [Ruminococcus] gauvreauii group, [Eubacterium] ventriosum CAG-352, Marvinbryantia markedly decreased. These shifts enhanced intestinal inflammation hepatic immune activation. Notably, Escherichia-Shigella may contribute LPS-TLR4/inflammasome activation, inflammatory cytokine release, reduced short-chain fatty acid (SCFA) production. Conversely, SCFA-producing bacteria Firmicutes showed protective role by mitigating lipid accumulation. Furthermore, disruption negatively correlated an increased Mediterraneibacter observed disturbances. As known produce ethanol, elevated exacerbate injury inflammation, potentially contributing development. Conclusion: reveals association dysbiosis, disruption, severity. Our findings suggest that disturbances influence modulating metabolic activity. highlight strategies, including modulation (e.g., light therapy, timed medication) microbiota-targeted interventions, slow or reverse progression.

Язык: Английский

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Effects of Deoxynivalenol Contamination on Growth Performance, Blood Biochemistry, Histology, Metabolomics, and the Microbiota: A Subacute Dose Oral Toxicity Study in Rats

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3086 - 3086

Опубликована: Март 27, 2025

Deoxynivalenol (DON), one of the most common mycotoxins, is frequently found in foods. This study investigated effects orally administered DON on blood biochemical parameters, growth performance, histology, microbial composition, and metabolism rats. After a 1-week adaptation period, 4-week-old rats were 0.9% saline (control), 1 mg/L (T1), 10 (T2), or 50 (T3) by gavage for 49 days. The DON-treated groups had significantly lower body weights than control group (p < 0.05). Blood alkaline phosphatase, phosphate, cholesterol, amylase, creatinine levels differed between With increasing doses, fibrosis apoptosis observed several tissues. In terms metabolites, bile acid biosynthesis pathway emerged as potential biomarker, while tryptophan was to be affected. fecal microbiota showed significant differences both alpha beta diversity cecal microbiota, relative abundance Firmicutes increased T1 groups, whereas Bacteroidota Campylobacterota more abundant T2 T3 groups. conclusion, our results that high exposure induces dose-dependent adverse

Язык: Английский

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Liver cirrhosis: current status and treatment options using western or traditional Chinese medicine

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июль 16, 2024

Liver cirrhosis arises from liver fibrosis and necroinflammation caused by various mechanisms of hepatic injury. It is a prevalent condition in clinical practice characterized hepatocellular dysfunction, portal hypertension, associated complications. Despite its common occurrence, the etiology pathogenesis remain incompletely understood, posing significant health threat. Effective prevention onset progression paramount medical research. Symptoms often include discomfort area, while complications such as sarcopenia, encephalopathy, ascites, upper gastrointestinal bleeding, infection can arise. While efficacy Western medicine treating uncertain, Chinese offers distinct advantages. This review explores advancements treatment encompassing non-pharmacological pharmacological modalities. interventions, including decoctions, patent medicines, acupuncture, exhibit notable reversal offer improved prognoses. Nowadays, combination also has considerable advantages, which worthy further research promotion. Standardized protocols based on these findings hold implications.

Язык: Английский

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Potential mechanisms of traditional Chinese medicine in the treatment of liver cirrhosis: a focus on gut microbiota

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Авг. 21, 2024

Cirrhosis, a pathological stage that develops from various chronic liver diseases, is characterized by fibrosis, pseudolobular formation, and inflammation. When it progresses to the decompensated phase, mortality rate of cirrhosis can reach 80%. The role gut microbiota in progression diseases has received significant attention. Numerous studies have shown regulating therapeutic effects on preventing reversing cirrhosis. This article reviewed mechanisms which influence cirrhosis, explaining effective traditional Chinese medicine. Through multi-directional regulation involving signaling pathways, diversity, restoration intestinal barrier function, medicine been promising ameliorating providing treatment options pharmacological guidance for occurrence development

Язык: Английский

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Effects of Hypericum attenuatum Choisy extract on the immunologic function and intestinal microflora of broilers under oxidative stress

Poultry Science, Год журнала: 2024, Номер 103(11), С. 104189 - 104189

Опубликована: Авг. 8, 2024

This study investigated the impact of Hypericum attenuatum Choisy extract (HYG) on immunological function and cecum microflora in broilers. A total 240 one-day-old AA broilers were randomly divided into 5 groups with 6 replicates 8 each: 1) CN group, which injected saline fed a basal diet; 2) PC lipolyaccharide (LPS) 3) HYG1 LPS 400 mg/kg HYG-supplemented 4) HYG2 800 5) HYG3 1,200 diet. Broilers 1 or same amount 12 hours before sampling d 21 42. The results revealed that dietary HYG supplementation alleviated spleen index thymus abnormalities, balanced disturbance serum immunoglobulin (Ig)M IgA levels, regulated cytokine balance serum, liver, jejunum tissues included induced by LPS. Dietary also downregulated relative expression inhibitor kappa B kinase alpha (IKKα) interleukin (IL)-6 mRNAs liver upregulated (IκBα) IL-10 spleen. improved cecal at 42 increasing abundance beneficial bacteria, such as Alistipes Phascolarctobacterium, while reducing harmful Helicobacter Colidextribacter. Spearman correlation analysis negative between activation NF-κB inhibitory pathway presence Erysipelatoclostridium, Subdoligranulum Parabacteroides. Conclusions: incorporation diet was optimal improving broiler function.

Язык: Английский

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Effects of Microencapsulated Essential Oils on Growth and Intestinal Health in Weaned Piglets

Animals, Год журнала: 2024, Номер 14(18), С. 2705 - 2705

Опубликована: Сен. 18, 2024

The study investigated the effects of microencapsulated essential oils (MEO) on growth performance, diarrhea, and intestinal microenvironment weaned piglets. 120 thirty-day-old piglets (Duroc × Landrace Yorkshire, 8.15 ± 0.07 kg) were randomly divided into four groups fed with a basal diet (CON) or CON containing 300 (L-MEO), 500 (M-MEO), 700 (H-MEO) mg/kg MEO, respectively, data related to performance measured. results revealed that MEO supplementation increased ADG ADFI in (

Язык: Английский

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Integrated evidence chain-based effectiveness evaluation of traditional Chinese medicines (iEC-Eff): A demonstration study

Acta Pharmaceutica Sinica B, Год журнала: 2024, Номер 15(2), С. 909 - 918

Опубликована: Дек. 26, 2024

Addressing the enduring challenge of evaluating traditional Chinese medicines (TCMs), integrated evidence chain-based effectiveness evaluation TCMs (Eff-iEC) has emerged. This paper explored its capacity through a demonstration study that evaluated six commonly used anti-hepatic fibrosis patent (CPMs), including Biejiajian Pill (BP), Dahuang Zhechong (DZP), Biejia Ruangan Compound (BRC), Fuzheng Huayu Capsule (FHC), Anluo Huaxian (AHP), and Heluo Shugan (HSC), using both Eff-iEC Grading Recommendations, Assessment, Development, Evaluation (GRADE) system. The recognition these CPMs within TCM academic community was also assessed their inclusion in relevant medical documents. Results showed BRC FHC received higher assessments GRADE system, while for others varied. Analysis revealed more accurately reflects clinical value CPMs, exhibiting superior evaluative capabilities. By breaking conventional pattern evaluation, offers novel epistemology better aligns with realities reasoning TCMs, providing coherent methodology decision-making, new drug evaluations, health policy formulation.

Язык: Английский

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Dahuang Zhechong Pill Improves Pulmonary Fibrosis through miR-29b-2-5p/HK2 Mediated Glycolysis Pathway

Chinese Journal of Integrative Medicine, Год журнала: 2024, Номер unknown

Опубликована: Сен. 4, 2024

Язык: Английский

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