Identification of the novel exhausted T cell CD8 + markers in breast cancer

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 19, 2024

Cancer is one of the most concerning public health issues and breast cancer common cancers in world. The immune cells within tumor microenvironment regulate development. In this study, single cell data sets were used to identify marker gene for exhausted CD8 + T (CD8Tex) cancer. Machine learning methods cluster subtypes establish prognostic models with bulk using evaluate impacts CD8Tex. We analyzed overexpressing survival-associated genes identified CD8Tex hub protein-protein-interaction network. relevance T-cells was evaluated. clinical associations sequencing spatial data. pan-cancer expression, survival, association analyzed. biomarker CD8Tex-based subtyping systems performed well separation patients different survival. CRTAM, CLEC2D, KLRB1 as demonstrated have potential therapy impact. This study provides a unique view critical therapy.

Язык: Английский

---

1,3,6-Trigalloylglucose: A Novel Potent Anti-Helicobacter pylori Adhesion Agent Derived from Aqueous Extracts of Terminalia chebula Retz

Molecules, Год журнала: 2024, Номер 29(5), С. 1161 - 1161

Опубликована: Март 5, 2024

1,3,6-Trigalloylglucose is a natural compound that can be extracted from the aqueous extracts of ripe fruit Terminalia chebula Retz, commonly known as “Haritaki”. The potential anti-Helicobacter pylori (HP) activity this has not been extensively studied or confirmed in scientific research. This was isolated using semi-preparative liquid chromatography (LC) system and identified through Ultra-high-performance chromatography–MS/MS (UPLC-MS/MS) Nuclear Magnetic Resonance (NMR). Its role evaluated Minimum inhibitory concentration (MIC) assay minimum bactericidal (MBC) assay, scanning electron microscope (SEM), inhibiting kinetics curves, urea fast test, Cell Counting Kit-8 (CCK-8) Western blot, Griess Reagent System. Results showed effectively inhibits growth HP strain ATCC 700392, damages structure, suppresses Cytotoxin-associated gene A (Cag A) protein, crucial factor infection. Importantly, it exhibits selective antimicrobial without impacting normal epithelial cells GES-1. In vitro studies have revealed acts an anti-adhesive agent, disrupting adhesion to host cells, critical step These findings underscore targeted therapeutic agent against infections.

Язык: Английский

---

Identification of molecular targets of Hypericum perforatum in blood for major depressive disorder: a machine-learning pharmacological study

Chinese Medicine, Год журнала: 2024, Номер 19(1)

Опубликована: Окт. 9, 2024

Major depressive disorder (MDD) is one of the most common psychiatric disorders worldwide. Hypericum perforatum (HP) a traditional herb that has been shown to have antidepressant effects, but its mechanism unclear. This study aims identify molecular targets HP for treatment MDD.

Язык: Английский

---

SCN3B is an Anti-breast Cancer Molecule with Migration Inhibition Effect

Biochemical Genetics, Год журнала: 2025, Номер unknown

Опубликована: Март 12, 2025

Язык: Английский

---

Chebulinic acid isolated from aqueous extracts of Terminalia chebula Retz inhibits Helicobacter pylori infection by potential binding to Cag A protein and regulating adhesion

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Окт. 2, 2024

Terminalia chebula Retz, known as the King of Tibet, is considered a functional food in China, celebrated for its antioxidant, immune-modulating, antibacterial, and anti-inflammatory properties. Chebulinic acid, derived from aqueous extracts However, potential an anti-Helicobacter pylori (HP) agent has not been fully explored. Herein, we extracted main compound Retz using semi-preparative liquid chromatography (LC) system identified 5 chebulinic acid through Ultra-high performance chromatography-MS/MS (UPLC-MS/MS) Nuclear Magnetic Resonance (NMR). To evaluate role, conducted minimum inhibitory concentration (MIC) bactericidal (MBC) assays, scanning electron microscope (SEM) imaging, inhibiting kinetics curves, urea fast test, cell counting kit-8 (CCK-8) assay, western blot analysis, griess reagent system, molecular docking. Our results showed that effectively inhibited growth HP strain ATCC 700392, damaged structure, exhibited selective antimicrobial activity without affecting normal epithelial cells GES-1. Importantly, it suppressed expression Cytotoxin-associated gene A (Cag A) protein, crucial factor infection. Molecular docking analysis predicted strong affinity (-9.7 kcal/mol) between Cag protein. Overall, our findings suggest acts anti-adhesive agent, disrupting adhesion to host cells, which critical step It also suppresses These highlight against infections.

Язык: Английский

---

Anti Helicobacter pylori Activity and Gastrointestinal Protective Effects of Terminalia bellirica: Mechanistic Insights from in Vitro and in Vivo Studies

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119569 - 119569

Опубликована: Март 1, 2025

Язык: Английский

---

Unveiling the therapeutic potential of HZQYF: exploring the inhibitory impact of a clinical herbal formula on gastric cancer through network pharmacology and transcript analysis

BMC Complementary Medicine and Therapies, Год журнала: 2025, Номер 25(1)

Опубликована: Апрель 17, 2025

Язык: Английский

---

In vitro anti-Helicobacter pylori activity and the underlining mechanism of an empirical herbal formula – Hezi Qingyou

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Фев. 19, 2024

Background Helicobacter pylori ( H. ) is thought to primarily colonize the human stomach and lead various gastrointestinal disorders, such as gastritis gastric cancer. Currently, main eradication treatment triple or quadruple therapy centered on antibiotics. Due antibiotic resistance, rate of decreasing gradually. Therefore, searching for anti- drugs from herbal sources has become a strategy treatment. Our team proposed Hezi Qingyou Formula (HZQYF), composed Chebulae Fructus, Ficus hirta Vahl Cloves, studied its activity mechanism. Methods Chemical components HZQYF were using UHPLC–MS/MS HPLC. Broth microdilution method agar dilution used evaluate HZQYF’s antibacterial activity. The effects expression adhesion genes alpA , alpB babA ), urease ureE ureF flagellar flaA, flaB explored Reverse Transcription-quantitative Polymerase Chain Reaction (RT-qPCR) technology. Effects morphology permeability extracellular membrane scanning electron microscopy (SEM) N-phenylnaphthalen-1-amine (NPN) uptake. Effect was kinetics analysis in vitro . Immunofluorescence staining examine effect adhesion. Western blot cagA protein. Results Minimum inhibitory concentration (MIC) values formula against clinical strains standard 80–160 μg/mL, minimum bactericidal (MBC) 160–320 μg/mL. could down-regulate alp A, flaA change increase permeability, decrease Conclusion Present studies confirmed that had promising activities demonstrated possible mechanism action by down-regulating bacterial adhesion, urease, gene expression, which provided scientific bases further investigations.

Язык: Английский

---

Effects of Bacterial Lysates on TLRs/NF-κB Pathway, Immune Function, and Prognosis in Children with Pulmonary Infection Caused by Streptococcus pneumoniae

Jundishapur Journal of Microbiology, Год журнала: 2024, Номер 17(8)

Опубликована: Окт. 20, 2024

Background: Bacterial lysates, as immunomodulators, may play a role in regulating the host immune response. Objectives: This study aimed to investigate effects of bacterial lysates OM85-BV treating pulmonary infections caused by Streptococcus pneumoniae children. Methods: A total 120 children with S. infection were divided into two groups: The control group and group, 1: 1 ratio. received intravenous mezlocillin at 100 mL/kg/d, while addition mezlocillin, orally 3.5 mg per dose. improvement clinical symptoms function indexes—including respiratory rate, tidal volume, volume peak expiratory flow proportion exhaled time time—were compared. Additionally, levels C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), soluble interleukin-2 receptor (sIL-2R), T lymphocyte subsets, immunoglobulins serum analyzed. Results: Compared showed significant decrease rate an increase flow, (P < 0.05 for all). also had significantly lower inflammatory factors such CRP, TNF-α, IL-6, sIL-2R. In terms function, increased subsets CD3+, CD4+, CD4+/CD8+ ratios, CD8+, well elevated IgA IgG levels. effective treatment was higher than 0.05). Conclusions: improves symptoms, lung reducing appears inhibit activation toll-like receptors (TLRs) nuclear factor kappa-B (NF-κB) pathway.

Язык: Английский

---

Association Between the Expression of T Helper Type 17 Cell-Related Cytokines and Valve Damage in Rheumatic Heart Disease

Molecular Biotechnology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 22, 2024

Язык: Английский

---