Chemical Composition of Mexicali Propolis and Its Effect on Gastric Repair in an Indomethacin-Induced Gastric Injury Murine Model

Antioxidants, Год журнала: 2025, Номер 14(1), С. 65 - 65

Опубликована: Янв. 8, 2025

Propolis is a resinous substance produced by bees that has several biomedical properties could contribute to the repair process of gastric mucosa, such as antioxidant, anti-inflammatory, healing, and gastroprotective properties. Thus, this study aimed determine chemical composition Mexicali propolis, its antioxidant capacity, effect on repair. Three polarity-directed extracts were obtained: ethanolic extract, ethyl acetate hexane extract. The activity, total phenolic content (TPC), flavone/flavonol determined for each was analysed using HPLC-TOF-MS (High-Performance Liquid Chromatography-Time-Of-Flight Mass Spectrometry) GC-MS (Gas Chromatography-Mass Spectrometry), 52 compounds identified. results revealed extract had greatest free radical scavenging bioactive compounds. On basis these results, propolis (MeEEP) subsequently evaluated. Prior evaluation, MeEEP found exhibit low oral toxicity, under Organisation Economic Co-operation Development (OECD) 425 guidelines. Gastric injury induced in male C57BL/6 mice intragastric administration indomethacin (10 mg/kg). (300 mg/kg) administered 6 h after induction daily thereafter. sacrificed at 12, 24, 48 assess effect. As result, enhanced lesion decreasing percentage bleeding area attenuating severity histological damage, demonstrated H&E staining. This associated with reduction MPO enzyme activity levels proinflammatory cytokines TNF-α, IL-1β, IL-6, maintaining controlled inflammation tissue. Furthermore, increased SOD enzymatic GSH levels, reducing degree oxidative damage tissue, MDA levels. Finally, evaluating apoptosis via immunohistochemistry, shown reduce expression proapoptotic marker Bax increase antiapoptotic Bcl-2. In conclusion, findings suggest may enhance through cytoprotective mechanism controlling exacerbation, stress, regulating apoptosis. These mechanisms are primarily attributed presence pinocembrin, tectochrysin, chrysin, apigenin, naringenin, acacetin, genistein, kaempferol. It important highlight provides preliminary exploration reparative Mexican describing potential action present propolis.

Язык: Английский

---

Spatially Resolved Metabolomics and Network Pharmacology Reveal Extract D Nephrotoxicity Mechanisms in Pleuropterus multiflorus Thunb.

Toxics, Год журнала: 2025, Номер 13(3), С. 182 - 182

Опубликована: Фев. 28, 2025

As a traditional Chinese medicine, the adverse hepatotoxicity effects of Pleuropterus multiflorus (Thunb.) Nakai (PM) have been documented. However, nephrotoxicity has neglected as studies related to kidney toxicity mechanisms are limited. Our previous research reported that extract D [95% ethanol (EtOH) elution, PM-D] in 70% EtOH PM showed more significant than other extracts. In current study, PM-D was continuously administered mice for 7 days at dose 2 g/kg (equivalent human 219.8 mg/kg), which increased renal biochemical indexes and caused pathological injury, suggesting toxicity. Therefore, network pharmacology spatially resolved metabolomics were conducted explore underpinning PM-D. Network indicated BCL2, HSP90, ESR1, CTNNB1 genes core targets, while phosphoinositide 3-kinase (PI3K)/protein kinase B(AKT)/signaling pathway significantly enriched. Spatially heterogeneous metabolite distribution kidney, further indicating nephrotoxic metabolic pathways enriched α-linolenic acid linoleic metabolism, pyrimidine carnitine synthesis, branched-chain fatty oxidation. comprehensive analyses highlighted oxidative stress apoptosis induced by disordered energy lipid metabolism issues, imbalanced nucleotide provide platform into mechanisms.

Язык: Английский

---

Protective effects of vincamine against ethanol-induced gastric ulcer by attenuation of IL-6, IL-1β, and TNF-α mRNA expression levels in the gastric mucosa of BALB/c mice

Journal of Molecular Histology, Год журнала: 2025, Номер 56(2)

Опубликована: Март 5, 2025

Язык: Английский

---

Study on Differential Metabolite Active Ingredients in Maize Roots Based on Network Pharmacology and Metabolomics Analysis

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Maize is a globally important crop. Roots are the main part of maize and mainly used for soil improvement maintaining crop growth as agricultural waste. Their application scope relatively small. It very to analyze components in roots order increase their resource utilization reduce burden waste disposal. Metabolomics shows that contain various bioactive components, such alkaloids, phenolic acids, flavonoids, etc. Therefore, this study explores potential pharmacological effects root metabolites under drought stress from perspective metabolomics combined with network pharmacology. The crude extraction betaine, metabolite stress, was conducted, anti-inflammatory antioxidant extract were evaluated. experimental results DPPH, Fenton, indicate betaine has certain effects, which provides basis its applications fields medicine food. research on extracting medicinal active substances not only economic environmental advantages but also significance promoting technological progress public health.

Язык: Английский

---

Synergistic effects of oleanolic acid and curcumin nanoparticles in gastric ulcer prevention

International Journal of Pharmaceutics, Год журнала: 2025, Номер unknown, С. 125465 - 125465

Опубликована: Март 1, 2025

Язык: Английский

---

Unlocking the potential of Radix Astragali and its active ingredients in gastric ulcer therapy

Journal of Asian Natural Products Research, Год журнала: 2025, Номер unknown, С. 1 - 15

Опубликована: Март 20, 2025

We studied the protective effects of Radix Astragali (RA) on gastric ulcer (GU). A literature search was conducted using databases from Web Science, PubMed, Springer, ScienceDirect, Science Direct Chinese National Knowledge Infrastructure (CNKI), and Wanfang. The inclusion criteria for this study were limited to reports RA, AS-IV, cycloastragenol, astragalus polysaccharide (APS), astragalosides (AST) in treatment ulcers. Any studies involving lesions that precancerous or cancerous eliminated. period database inception through June 2024. results suggested RA hold promiseas potential novel therapeutics therapy GU.

Язык: Английский

---

Ingenol ameliorates silicosis via targeting the PTGS2/PI3K/AKT signaling axis: Implications for therapeutic intervention

Cellular Signalling, Год журнала: 2025, Номер unknown, С. 111780 - 111780

Опубликована: Март 1, 2025

Язык: Английский

---

The Role of Triterpenoids in Gastric Ulcer: Mechanisms and Therapeutic Potentials

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3237 - 3237

Опубликована: Март 31, 2025

Gastric ulcer (GU) is a prevalent gastrointestinal disorder impacting millions worldwide, with complex pathogenic mechanisms that may progress to severe illnesses. Conventional therapies relying on anti-secretory agents and antibiotics are constrained by drug abuse increased bacterial resistance, highlighting the urgent need for safer therapeutic alternatives. Natural medicinal compounds, particularly triterpenoids derived from plants herbs, have gained significant attention in recent years due their favorable efficacy reduced toxicity profiles. Emerging evidence indicates exhibit potent anti-ulcer properties across various experimental models, modulating key pathways involved inflammation, oxidative stress, apoptosis, mucosal protection. Integrating current knowledge of these bioactive compounds facilitates development natural triterpenoids, addresses challenges clinical translation, deepens mechanistic understanding GU pathogenesis, drives innovation strategies GU. This review comprehensively evaluates research treatment since 2000, discussing biological sources, structural characteristics, pharmacological activities, action, animal models employed studies, limitations, associated application.

Язык: Английский

---

Integrating metabolomics and network pharmacology to investigate Mu Jin Powder prevents ethanol-induced gastric ulcer in rats

Journal of Ethnopharmacology, Год журнала: 2025, Номер unknown, С. 119730 - 119730

Опубликована: Апрель 1, 2025

Язык: Английский

---

The crucial role of neutrophil extracellular traps and IL-17 signaling in indomethacin-induced gastric injury in mice

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Апрель 9, 2025

Язык: Английский

---