TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 181, С. 118046 - 118046
Опубликована: Ноя. 8, 2024
Язык: Английский
Clinical Microbiology Reviews, Год журнала: 2023, Номер 37(1)
Опубликована: Дек. 14, 2023
SUMMARY Wastewater-based surveillance (WBS) has undergone dramatic advancement in the context of coronavirus disease 2019 (COVID-19) pandemic. The power and potential this platform technology were rapidly realized when it became evident that not only did WBS-measured SARS-CoV-2 RNA correlate strongly with COVID-19 clinical within monitored populations but also, fact, functioned as a leading indicator. Teams from across globe innovated novel approaches by which wastewater could be collected diverse sewersheds ranging treatment plants (enabling community-level surveillance) to more granular locations including individual neighborhoods high-risk buildings such long-term care facilities (LTCF). Efficient processes enabled extraction concentration highly dilute matrix. Molecular genomic tools identify, quantify, characterize its various variants adapted programs applied these mixed environmental systems. Novel data-sharing allowed information mobilized made immediately available public health government decision-makers even public, enabling evidence-informed decision-making based on local dynamics. WBS since been recognized tool transformative potential, providing near-real-time cost-effective, objective, comprehensive, inclusive data changing prevalence measured analytes space time populations. However, consequence rapid innovation hundreds teams simultaneously, tremendous heterogeneity currently exists literature. This manuscript provides state-of-the-art review established details current work underway expanding scope other infectious targets.
Язык: Английский
Процитировано
68
The Science of The Total Environment, Год журнала: 2023, Номер 878, С. 162953 - 162953
Опубликована: Март 21, 2023
Язык: Английский
Процитировано
65
Current Opinion in Environmental Science & Health, Год журнала: 2023, Номер 33, С. 100458 - 100458
Опубликована: Март 7, 2023
Язык: Английский
Процитировано
31
Nature Biomedical Engineering, Год журнала: 2023, Номер 7(12), С. 1636 - 1648
Опубликована: Сен. 21, 2023
Язык: Английский
Процитировано
30
The Science of The Total Environment, Год журнала: 2024, Номер 918, С. 170636 - 170636
Опубликована: Фев. 6, 2024
Wastewater-based surveillance constitutes a valuable methodology for the continuous monitoring of viral circulation, with capacity to function as an early warning system. It holds particular significance in scenarios where respiratory viruses exhibit overlapping clinical presentations, occurs SARS-CoV-2, influenza virus (IV), and syncytial (RSV), allows seasonal outbreaks be distinguished from COVID-19 peaks. Furthermore, sewage sludge, given it harbors concentrated human waste large population, serves substantial reservoir pathogen detection. To effectively integrate wastewater-based epidemiology into infectious disease surveillance, detection methods employed wastewater samples must adapted distinct characteristics sludge matrices. In this study, we applied protocols IV RSV comparing their performance results obtained wastewater. assess efficiency these protocols, were spiked RNA, either free or incorporated lentiviral particles. Samples using aluminum hydroxide adsorption-precipitation method before RNA extraction. Absolute quantification was carried out by RT-qPCR, including internal control monitor potential inhibitory factors. Recovery efficiencies both 60 % 75 71 respectively wastewater, whereas values particles 16 10 21 17 respectively. Additionally, protocol enabled naturally occurring collected two treatment plants during winter months, thus affirming efficacy methodologies.
Язык: Английский
Процитировано
8
Biosensors and Bioelectronics, Год журнала: 2024, Номер 263, С. 116617 - 116617
Опубликована: Июль 30, 2024
Язык: Английский
Процитировано
6
Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(23), С. 63323 - 63334
Опубликована: Март 29, 2023
Язык: Английский
Процитировано
10
Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(31), С. 76687 - 76701
Опубликована: Май 27, 2023
Язык: Английский
Процитировано
10
Environmental Science and Pollution Research, Год журнала: 2023, Номер 30(32), С. 79315 - 79334
Опубликована: Июнь 7, 2023
Abstract Wastewater-based epidemiology has been widely used as a cost-effective method for tracking the COVID-19 pandemic at community level. Here we describe COVIDBENS, wastewater surveillance program running from June 2020 to March 2022 in treatment plant of Bens A Coruña (Spain). The main goal this work was provide an effective early warning tool based help decision-making both social and public health levels. RT-qPCR procedures Illumina sequencing were weekly monitor viral load detect SARS-CoV-2 mutations wastewater, respectively. In addition, own statistical models applied estimate real number infected people frequency each emerging variant circulating community, which considerable improved strategy. Our analysis detected 6 waves with concentrations between 10 3 RNA copies/L. system able anticipate outbreaks during 8–36 days advance respect clinical reports and, emergence new variants such Alpha (B.1.1.7), Delta (B.1.617.2), Omicron (B.1.1.529 BA.2) 42, 30, 27 days, respectively, before did. Data generated here helped local authorities managers give faster more efficient response situation, also allowed important industrial companies adapt their production situation. wastewater-based developed our metropolitan area (Spain) served powerful combining monitoring over time.
Язык: Английский
Процитировано
10
The Science of The Total Environment, Год журнала: 2023, Номер 908, С. 167966 - 167966
Опубликована: Окт. 28, 2023
The lack of standardized methods and large differences in virus concentration extraction workflows have hampered severe acute respiratory syndrome (SARS-CoV-2) wastewater surveillance data reporting practices. Numerous studies shown that adsorption-extraction (AE) method holds promise, yet several uncertainties remain regarding the optimal AE workflow. Several procedural components may influence recovered concentrations target DNA/RNA, including membrane types, homogenization instruments, speed duration, lysis buffer. In this study, 42 different varied these were compared to determine by quantifying endogenous SARS-CoV-2, human adenovirus (HAdV 40/41) a bacterial marker gene fecal pollution (Bacteroides HF183). Our findings suggest certain selected workflow had significant impact on SARS-CoV-2 concentrations, whereas it minimal HF183 no effect HAdV 40/41 concentrations. When comparing individual workflow, such as type (MF-Millipore™ 0.45 μm MCE vs. Isopore™ 0.40 μm) instruments (Precellys 24 homogenizer Vortex-Genie®-2), we found they 40/41, This suggests at least some consumables equipment are interchangeable. Buffer PM1 + TRIzol based yielded higher than other workflows. without chloroform. Similarly, speeds (5000-10,000 rpm) led increased but 40/41. indicate minor enhancements can improve recovery viruses bacteria from wastewater, leading outcomes efforts.
Язык: Английский
Процитировано
9