Unveiling the Dynamics of Antimicrobial Resistance: A Year-Long Surveillance (2023) at the Largest Infectious Disease Profile Hospital in Western Romania DOI Creative Commons
Sorina Maria Denisa Laitin, Luminița Bădițoiu,

Ruxandra Laza

и другие.

Antibiotics, Год журнала: 2024, Номер 13(12), С. 1130 - 1130

Опубликована: Ноя. 25, 2024

Background/Objectives: Antimicrobial resistance (AMR) is a critical global health threat, leading to increased morbidity, mortality, and healthcare costs. This study aimed identify the most common bacterial pathogens their profiles from 2179 positive clinical cultures inpatients at “Victor Babes” Hospital of Infectious Disease Pneumoftiziology Timisoara in 2023. Methods: Samples were collected sputum, bronchial aspiration, hemoculture, urine, wound secretions, catheter samples, other specimens. Results: Key identified included Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, with notable patterns, observed K. pneumoniae exhibited high rates, notably 41.41% Quarter 1, while E. coli showed 35.93% same period. S. particularly MRSA, remained persistent challenge, 169 cases recorded over year. A. baumannii P. aeruginosa displayed alarming levels multi-drug resistance, especially 3 (88.24% 22.02%, respectively). Although there was general decline rates by 4, such as aureus continued exhibit significant (81.25% 21.74%, Conclusions: The study’s findings align broader antimicrobial trends Romania, where (MRSA), Acinetobacter, species have been widely reported, reflecting country’s ongoing struggle multi-drug-resistant infections. Despite some reductions across quarters, presence these resistant strains underscores need for strengthened stewardship, infection control measures, continuous surveillance combat growing threat AMR Romania similar settings.

Язык: Английский

Unveiling soil-borne antibiotic resistome and their associated risks: A comparative study of antibiotic and non-antibiotic pharmaceutical factories DOI

Liusheng Lei,

Jing Yu,

Linjia Liu

и другие.

Journal of Hazardous Materials, Год журнала: 2025, Номер 486, С. 137127 - 137127

Опубликована: Янв. 5, 2025

Язык: Английский

Процитировано

2
Systematic review and meta-analysis on the carbapenem-resistant hypervirulent Klebsiella pneumoniae isolates DOI Creative Commons

Motahareh Sabaghi Qala Nou,

Zahra Amirian, Fatemeh Dehghani

и другие.

BMC Pharmacology and Toxicology, Год журнала: 2025, Номер 26(1)

Опубликована: Янв. 30, 2025

The global dissemination of carbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) poses a critical threat to public health. However, comprehensive data on the prevalence and resistance rates CR-hvKp are limited. This systematic review meta-analysis aim estimate pooled carbapenem among hvKp strains assess distribution carbapenemase genes. A search ISI Web Science, PubMed, Google Scholar was conducted identify studies reporting in strains. genes calculated using event with 95% confidence intervals. total 36 encompassing 1,098 were included. 49% for imipenem, 53.2% meropenem, 38.2% ertapenem. Carbapenemase gene 19.1% blaVIM, 22.0% blaNDM, 43.4% blaOXA-48, 58.8% blaKPC. high widespread underscore their significant These findings highlight urgent need enhanced surveillance, rapid diagnostic tools, stringent infection control measures mitigate spread CR-hvKp. Future research should focus understanding mechanisms developing targeted therapeutic strategies address this challenge.

Язык: Английский

Процитировано

0
Genomic Insights into Halo KS-7: An AI-Driven Characterization of a Novel Halophilic Bacteriophage Targeting Carbapenem-Resistant Klebsiella pneumoniae DOI

Sahar Abed,

Masoumeh Beig, Shahin Soltani

и другие.

Опубликована: Май 13, 2025

Abstract Carbapenem-resistant Klebsiella pneumoniae(CRKP) is a multidrug-resistant (MDR) pathogen causing severe infections in immunocompromised patients, prompting the exploration of alternative therapies like bacteriophage therapy. In this study, we isolated and characterized novel halophilic lytic bacteriophage, Halo KS-7, targeting K. pneumoniae, used an AI-driven annotation pipeline Python to analyze its genome therapeutic potential. Bacteriophages were from Hospital wastewater, purified through plaque isolation, confirmed using double-layer agar method. Morphological analysis via transmission electron microscopy (TEM) assays assessed activity. DNA sequencing was done Illumina HiSeq 2000, followed by assembly, AI-guided annotation, gene prediction, protein function classification, comparative genomics CLC Genomics Workbench. We also evaluated host range, temperature stability, pH sensitivity, salt stress tolerance assess KS-7 exhibited strong activity against CRKP classified as Myoviridae TEM. Phenotypic demonstrated optimal at 37 °C neutral pH, effective 4–10, enhanced performance high-salinity conditions. Its 58.716 kb linear dsDNA (44.4% G+C) contains 49 predicted ORFs, lacks integrase, lysogeny, or antibiotic-resistance genes, includes three tRNA genes (tRNATyr, tRNAPro, tRNAAsn). It toxin auxiliary factors MazG, pyrophosphatase, HNH endonucleases that enhance bacterial killing without promoting horizontal transfer resistance. Functional assigned ~65% ORFs structural, replication, packaging roles. Comparative showed moderate similarity other but with distinct accessory features, emphasizing novelty value. novel, strictly antibacterial resilience, supporting use promising biocontrol agent potential for clinical development managing MDR infections.

Язык: Английский

Процитировано

0
Variation of healthcare associated infections at a tertiary hospital in Southwest China over a 5-year period (2019–2023): a retrospective observational study DOI Creative Commons

Danyan Liang,

Wei Liu, Yao Zhong

и другие.

BMC Infectious Diseases, Год журнала: 2025, Номер 25(1)

Опубликована: Июнь 2, 2025

Healthcare-associated infections (HAIs), which are associated with prolonged hospitalization and increased mortality, remain a global challenge. The COVID-19 pandemic paradoxically reduced some HAIs through enhanced hygiene measures but exacerbated others due to resource diversion, effects after policy changes remaining unclear. Therefore, this study analyzed distribution, pathogenic microorganisms antibiotic susceptibility capturing the period surrounding strict control in southwest China tertiary hospital. We conducted retrospective at hospital from 2019 2023. Data were extracted real-time surveillance system, where initially diagnosed by clinicians subsequently verified infection personnel. HAI incidence rate was calculated per 1000 stays, negative-binomial regression used compare rates across years. This enrolled 2808 cases, 1665 males 1143 females, averaging 61.37 years old. of 2023 1.75, 1.12, 0.98, 1.31, 1.30 respectively. Hematology (323, 11.50%), cardiology (309, 11.00%), neurology (262, 9.33%) top three departments highest rates. Lower respiratory tract (1198, 42.66%), bloodstream (419, 14.92%), urinary (406, 14.46%) most common sites. Klebsiella pneumoniae (173, 15.22%), Escherichia coli (155, 13.63%), Acinetobacter baumannii (136, 11.96%) frequent microorganism. resistant antibiotics. cefuroxime Axetil, ceftriaxone. sulfamethoxazole trimethoprim, ceftriaxone cefuroxime. These all susceptible tigecycline. fluctuated over years, peaking 2019, suggesting potential shifts dynamics. pneumoniae, coli, predominant pathogens, tigecycline may be considered as option against these pathogens. highlights importance enhancing high-risk sites, optimizing stewardship, continuously monitoring trends inform evidence-based policies. Not applicable.

Язык: Английский

Процитировано

0
Unveiling the Dynamics of Antimicrobial Resistance: A Year-Long Surveillance (2023) at the Largest Infectious Disease Profile Hospital in Western Romania DOI Creative Commons
Sorina Maria Denisa Laitin, Luminița Bădițoiu,

Ruxandra Laza

и другие.

Antibiotics, Год журнала: 2024, Номер 13(12), С. 1130 - 1130

Опубликована: Ноя. 25, 2024

Background/Objectives: Antimicrobial resistance (AMR) is a critical global health threat, leading to increased morbidity, mortality, and healthcare costs. This study aimed identify the most common bacterial pathogens their profiles from 2179 positive clinical cultures inpatients at “Victor Babes” Hospital of Infectious Disease Pneumoftiziology Timisoara in 2023. Methods: Samples were collected sputum, bronchial aspiration, hemoculture, urine, wound secretions, catheter samples, other specimens. Results: Key identified included Klebsiella pneumoniae, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Acinetobacter baumannii, with notable patterns, observed K. pneumoniae exhibited high rates, notably 41.41% Quarter 1, while E. coli showed 35.93% same period. S. particularly MRSA, remained persistent challenge, 169 cases recorded over year. A. baumannii P. aeruginosa displayed alarming levels multi-drug resistance, especially 3 (88.24% 22.02%, respectively). Although there was general decline rates by 4, such as aureus continued exhibit significant (81.25% 21.74%, Conclusions: The study’s findings align broader antimicrobial trends Romania, where (MRSA), Acinetobacter, species have been widely reported, reflecting country’s ongoing struggle multi-drug-resistant infections. Despite some reductions across quarters, presence these resistant strains underscores need for strengthened stewardship, infection control measures, continuous surveillance combat growing threat AMR Romania similar settings.

Язык: Английский

Процитировано

1