Unveiling soil-borne antibiotic resistome and their associated risks: A comparative study of antibiotic and non-antibiotic pharmaceutical factories
Liusheng Lei,
Jing Yu,
Linjia Liu
и другие.
Journal of Hazardous Materials,
Год журнала:
2025,
Номер
486, С. 137127 - 137127
Опубликована: Янв. 5, 2025
Язык: Английский
Systematic review and meta-analysis on the carbapenem-resistant hypervirulent Klebsiella pneumoniae isolates
BMC Pharmacology and Toxicology,
Год журнала:
2025,
Номер
26(1)
Опубликована: Янв. 30, 2025
The
global
dissemination
of
carbapenem-resistant
hypervirulent
Klebsiella
pneumoniae
(CR-hvKp)
poses
a
critical
threat
to
public
health.
However,
comprehensive
data
on
the
prevalence
and
resistance
rates
CR-hvKp
are
limited.
This
systematic
review
meta-analysis
aim
estimate
pooled
carbapenem
among
hvKp
strains
assess
distribution
carbapenemase
genes.
A
search
ISI
Web
Science,
PubMed,
Google
Scholar
was
conducted
identify
studies
reporting
in
strains.
genes
calculated
using
event
with
95%
confidence
intervals.
total
36
encompassing
1,098
were
included.
49%
for
imipenem,
53.2%
meropenem,
38.2%
ertapenem.
Carbapenemase
gene
19.1%
blaVIM,
22.0%
blaNDM,
43.4%
blaOXA-48,
58.8%
blaKPC.
high
widespread
underscore
their
significant
These
findings
highlight
urgent
need
enhanced
surveillance,
rapid
diagnostic
tools,
stringent
infection
control
measures
mitigate
spread
CR-hvKp.
Future
research
should
focus
understanding
mechanisms
developing
targeted
therapeutic
strategies
address
this
challenge.
Язык: Английский
Genomic Insights into Halo KS-7: An AI-Driven Characterization of a Novel Halophilic Bacteriophage Targeting Carbapenem-Resistant Klebsiella pneumoniae
Опубликована: Май 13, 2025
Abstract
Carbapenem-resistant
Klebsiella
pneumoniae(CRKP)
is
a
multidrug-resistant
(MDR)
pathogen
causing
severe
infections
in
immunocompromised
patients,
prompting
the
exploration
of
alternative
therapies
like
bacteriophage
therapy.
In
this
study,
we
isolated
and
characterized
novel
halophilic
lytic
bacteriophage,
Halo
KS-7,
targeting
K.
pneumoniae,
used
an
AI-driven
annotation
pipeline
Python
to
analyze
its
genome
therapeutic
potential.
Bacteriophages
were
from
Hospital
wastewater,
purified
through
plaque
isolation,
confirmed
using
double-layer
agar
method.
Morphological
analysis
via
transmission
electron
microscopy
(TEM)
assays
assessed
activity.
DNA
sequencing
was
done
Illumina
HiSeq
2000,
followed
by
assembly,
AI-guided
annotation,
gene
prediction,
protein
function
classification,
comparative
genomics
CLC
Genomics
Workbench.
We
also
evaluated
host
range,
temperature
stability,
pH
sensitivity,
salt
stress
tolerance
assess
KS-7
exhibited
strong
activity
against
CRKP
classified
as
Myoviridae
TEM.
Phenotypic
demonstrated
optimal
at
37
°C
neutral
pH,
effective
4–10,
enhanced
performance
high-salinity
conditions.
Its
58.716
kb
linear
dsDNA
(44.4%
G+C)
contains
49
predicted
ORFs,
lacks
integrase,
lysogeny,
or
antibiotic-resistance
genes,
includes
three
tRNA
genes
(tRNATyr,
tRNAPro,
tRNAAsn).
It
toxin
auxiliary
factors
MazG,
pyrophosphatase,
HNH
endonucleases
that
enhance
bacterial
killing
without
promoting
horizontal
transfer
resistance.
Functional
assigned
~65%
ORFs
structural,
replication,
packaging
roles.
Comparative
showed
moderate
similarity
other
but
with
distinct
accessory
features,
emphasizing
novelty
value.
novel,
strictly
antibacterial
resilience,
supporting
use
promising
biocontrol
agent
potential
for
clinical
development
managing
MDR
infections.
Язык: Английский
Variation of healthcare associated infections at a tertiary hospital in Southwest China over a 5-year period (2019–2023): a retrospective observational study
BMC Infectious Diseases,
Год журнала:
2025,
Номер
25(1)
Опубликована: Июнь 2, 2025
Healthcare-associated
infections
(HAIs),
which
are
associated
with
prolonged
hospitalization
and
increased
mortality,
remain
a
global
challenge.
The
COVID-19
pandemic
paradoxically
reduced
some
HAIs
through
enhanced
hygiene
measures
but
exacerbated
others
due
to
resource
diversion,
effects
after
policy
changes
remaining
unclear.
Therefore,
this
study
analyzed
distribution,
pathogenic
microorganisms
antibiotic
susceptibility
capturing
the
period
surrounding
strict
control
in
southwest
China
tertiary
hospital.
We
conducted
retrospective
at
hospital
from
2019
2023.
Data
were
extracted
real-time
surveillance
system,
where
initially
diagnosed
by
clinicians
subsequently
verified
infection
personnel.
HAI
incidence
rate
was
calculated
per
1000
stays,
negative-binomial
regression
used
compare
rates
across
years.
This
enrolled
2808
cases,
1665
males
1143
females,
averaging
61.37
years
old.
of
2023
1.75,
1.12,
0.98,
1.31,
1.30
respectively.
Hematology
(323,
11.50%),
cardiology
(309,
11.00%),
neurology
(262,
9.33%)
top
three
departments
highest
rates.
Lower
respiratory
tract
(1198,
42.66%),
bloodstream
(419,
14.92%),
urinary
(406,
14.46%)
most
common
sites.
Klebsiella
pneumoniae
(173,
15.22%),
Escherichia
coli
(155,
13.63%),
Acinetobacter
baumannii
(136,
11.96%)
frequent
microorganism.
resistant
antibiotics.
cefuroxime
Axetil,
ceftriaxone.
sulfamethoxazole
trimethoprim,
ceftriaxone
cefuroxime.
These
all
susceptible
tigecycline.
fluctuated
over
years,
peaking
2019,
suggesting
potential
shifts
dynamics.
pneumoniae,
coli,
predominant
pathogens,
tigecycline
may
be
considered
as
option
against
these
pathogens.
highlights
importance
enhancing
high-risk
sites,
optimizing
stewardship,
continuously
monitoring
trends
inform
evidence-based
policies.
Not
applicable.
Язык: Английский
Unveiling the Dynamics of Antimicrobial Resistance: A Year-Long Surveillance (2023) at the Largest Infectious Disease Profile Hospital in Western Romania
Antibiotics,
Год журнала:
2024,
Номер
13(12), С. 1130 - 1130
Опубликована: Ноя. 25, 2024
Background/Objectives:
Antimicrobial
resistance
(AMR)
is
a
critical
global
health
threat,
leading
to
increased
morbidity,
mortality,
and
healthcare
costs.
This
study
aimed
identify
the
most
common
bacterial
pathogens
their
profiles
from
2179
positive
clinical
cultures
inpatients
at
“Victor
Babes”
Hospital
of
Infectious
Disease
Pneumoftiziology
Timisoara
in
2023.
Methods:
Samples
were
collected
sputum,
bronchial
aspiration,
hemoculture,
urine,
wound
secretions,
catheter
samples,
other
specimens.
Results:
Key
identified
included
Klebsiella
pneumoniae,
Escherichia
coli,
Staphylococcus
aureus,
Pseudomonas
aeruginosa,
Acinetobacter
baumannii,
with
notable
patterns,
observed
K.
pneumoniae
exhibited
high
rates,
notably
41.41%
Quarter
1,
while
E.
coli
showed
35.93%
same
period.
S.
particularly
MRSA,
remained
persistent
challenge,
169
cases
recorded
over
year.
A.
baumannii
P.
aeruginosa
displayed
alarming
levels
multi-drug
resistance,
especially
3
(88.24%
22.02%,
respectively).
Although
there
was
general
decline
rates
by
4,
such
as
aureus
continued
exhibit
significant
(81.25%
21.74%,
Conclusions:
The
study’s
findings
align
broader
antimicrobial
trends
Romania,
where
(MRSA),
Acinetobacter,
species
have
been
widely
reported,
reflecting
country’s
ongoing
struggle
multi-drug-resistant
infections.
Despite
some
reductions
across
quarters,
presence
these
resistant
strains
underscores
need
for
strengthened
stewardship,
infection
control
measures,
continuous
surveillance
combat
growing
threat
AMR
Romania
similar
settings.
Язык: Английский