Phosphorothioate-modified G-quadruplex as a signal-on dual-mode reporter for CRISPR/Cas12a-based portable detection of environmental pollutants

Analytica Chimica Acta, Год журнала: 2024, Номер 1308, С. 342649 - 342649

Опубликована: Апрель 25, 2024

Язык: Английский

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CRISPR-Cas12a-mediated colorimetric aptasensor of netilmicin based on enzymes-assisted signal amplification and nanozyme employing a rationally truncated aptamer

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 414, С. 135969 - 135969

Опубликована: Май 12, 2024

Язык: Английский

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CRISPR/Cas systems for genomic Editing, biochemical Sensing, Bioanalysis, and diagnostics

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 112638 - 112638

Опубликована: Янв. 1, 2025

Язык: Английский

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Comprehensive Approaches to Expending CRISPR Biosensor Applications Beyond Nucleic Acid Targets

TrAC Trends in Analytical Chemistry, Год журнала: 2025, Номер unknown, С. 118179 - 118179

Опубликована: Фев. 1, 2025

Язык: Английский

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Screening and Diagnosis of Colorectal Cancer Using Nucleic Acid Aptamers Targeting Solobacterium moorei: Development of Electrochemical Sensors for Clinical Application

Sensors and Actuators B Chemical, Год журнала: 2025, Номер unknown, С. 137669 - 137669

Опубликована: Март 1, 2025

Язык: Английский

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CRISPR/Cas12a regulated dual-channel ratiometric fluorescence biosensing for sensitive and accurate detection of kanamycin

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113581 - 113581

Опубликована: Апрель 1, 2025

Язык: Английский

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CRISPR/Cas12a‐loaded intelligent DNA hydrogel for universal and ultrasensitive exosome assay

View, Год журнала: 2024, Номер 5(2)

Опубликована: Фев. 1, 2024

Abstract Tumor‐derived exosomes are crucial for early non‐invasive and accurate tumor diagnosis in clinical diagnostics. The development of highly sensitive, simple, intuitive exosome assays has sparked a research upsurge Here, we develop bio‐responsive intelligent DNA hydrogel loaded with CRISPR/Cas12a universal ultrasensitive detection the exosomes. aptamer serves as target response unit switch, competitively disintegrating region linkers then Cas12a/crRNA was released activated, resulting high fluorescent intensity at limit 119 particles/μL. Moreover, constructed colorimetric tube is made by loading filter membrane on lid bottom, which enables one‐pot portable detection. Without need laboratory instruments professionals, this strategy allows naked eye low 10 4 particles/μL, shows great applicability distinguishing between healthy individuals, pretreatment patients, post‐treatment patients after obtaining testable analyte. In study, an platform that one‐step sensing dual signal output introduced. findings here suggest promising tool application liquid biopsy based diagnosis.

Язык: Английский

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Near-infrared light-induced homogeneous photoelectrochemical biosensor based on 3D walking nanomotor-assisted CRISPR/Cas12a for ultrasensitive microRNA-155 detection

Journal of Colloid and Interface Science, Год журнала: 2024, Номер 667, С. 82 - 90

Опубликована: Апрель 3, 2024

Язык: Английский

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Intelligent DNA-based hydrogels: An outstanding programable and biocompatible material for food applications

Trends in Food Science & Technology, Год журнала: 2024, Номер 149, С. 104546 - 104546

Опубликована: Май 17, 2024

Язык: Английский

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Bacteria proliferation-mediated cascade amplification for visually ultrasensitive detection of extracellular vesicles

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 408, С. 135526 - 135526

Опубликована: Фев. 22, 2024

Язык: Английский

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