Recent advances of food safety detection by nucleic acid isothermal amplification integrated with CRISPR/Cas

Critical Reviews in Food Science and Nutrition, Journal Year: 2023, Volume and Issue: 64(32), P. 12061 - 12082

Published: Sept. 10, 2023

Food safety problems have become one of the most important public health issues worldwide. Therefore, development rapid, effective and robust detection is great importance. Amongst a range methods, nucleic acid isothermal amplification (NAIA) plays role in food detection. However, widespread application remains limited due to few shortcomings. CRISPR/Cas system has emerged as powerful tool detection, which could be readily integrated with NAIA improve sensitivity, specificity, adaptability versatility dependability. currently there was lack comprehensive summary regarding integration field In this review, recent advances based on CRISPR/Cas-integrated were comprehensively reviewed. To begin with, summarized. Then, types working principles introduced. The applications for mainly introduced objectively discussed. Lastly, current challenges future opportunities proposed. summary, technology expected an approach leading safer more reliable industry.

Language: Английский

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Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(11), P. 3988 - 4007

Published: Oct. 23, 2023

Point-of-care (POC) detection is getting more and attention in many fields due to its accuracy on-site test property. The CRISPR/Cas12a system endowed with excellent sensitivity, target identification specificity, signal amplification ability biosensing because of unique trans-cleavage ability. As a result, lot research has been made develop CRISPR/Cas12a-based biosensors. In this review, we focused on readout strategies summarized recent sensitivity-improving fluorescence, colorimetric, electrochemical signaling. Then introduced novel portability-improving based lateral flow assays (LFAs), microfluidic chips, simplified instruments, one-pot design. the end, also provide our outlook for future development

Language: Английский

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Near-infrared light-induced homogeneous photoelectrochemical biosensor based on 3D walking nanomotor-assisted CRISPR/Cas12a for ultrasensitive microRNA-155 detection

Journal of Colloid and Interface Science, Journal Year: 2024, Volume and Issue: 667, P. 82 - 90

Published: April 3, 2024

Language: Английский

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Phosphorothioate-modified G-quadruplex as a signal-on dual-mode reporter for CRISPR/Cas12a-based portable detection of environmental pollutants

Analytica Chimica Acta, Journal Year: 2024, Volume and Issue: 1308, P. 342649 - 342649

Published: April 25, 2024

Language: Английский

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CRISPR/Cas12a‐loaded intelligent DNA hydrogel for universal and ultrasensitive exosome assay

View, Journal Year: 2024, Volume and Issue: 5(2)

Published: Feb. 1, 2024

Abstract Tumor‐derived exosomes are crucial for early non‐invasive and accurate tumor diagnosis in clinical diagnostics. The development of highly sensitive, simple, intuitive exosome assays has sparked a research upsurge Here, we develop bio‐responsive intelligent DNA hydrogel loaded with CRISPR/Cas12a universal ultrasensitive detection the exosomes. aptamer serves as target response unit switch, competitively disintegrating region linkers then Cas12a/crRNA was released activated, resulting high fluorescent intensity at limit 119 particles/μL. Moreover, constructed colorimetric tube is made by loading filter membrane on lid bottom, which enables one‐pot portable detection. Without need laboratory instruments professionals, this strategy allows naked eye low 10 4 particles/μL, shows great applicability distinguishing between healthy individuals, pretreatment patients, post‐treatment patients after obtaining testable analyte. In study, an platform that one‐step sensing dual signal output introduced. findings here suggest promising tool application liquid biopsy based diagnosis.

Language: Английский

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Bacteria proliferation-mediated cascade amplification for visually ultrasensitive detection of extracellular vesicles

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 408, P. 135526 - 135526

Published: Feb. 22, 2024

Language: Английский

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CRISPR-Cas12a-mediated colorimetric aptasensor of netilmicin based on enzymes-assisted signal amplification and nanozyme employing a rationally truncated aptamer

Sensors and Actuators B Chemical, Journal Year: 2024, Volume and Issue: 414, P. 135969 - 135969

Published: May 12, 2024

Language: Английский

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Intelligent DNA-based hydrogels: An outstanding programable and biocompatible material for food applications

Trends in Food Science & Technology, Journal Year: 2024, Volume and Issue: 149, P. 104546 - 104546

Published: May 17, 2024

Language: Английский

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CRISPR/Cas systems combined with DNA nanostructures for biomedical applications

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(23), P. 3098 - 3117

Published: Jan. 1, 2024

DNA nanostructures are easy to design and construct, have good biocompatibility, show great potential in biosensing drug delivery. Numerous distinctive versatile been developed explored for biomedical applications. In addition that completely assembled from DNA, composite obtained by combining with other organic or inorganic materials also widely used related research. The CRISPR/Cas system has attracted attention as a powerful gene editing technology is diagnosis. Many researchers committed exploring new possibilities systems. These explorations provide support the development of detection methods cargo delivery pathways, inspiration improving relevant platforms, further expand application scope This paper mainly reviews principles applications combined based on types nanostructures. Finally, status, challenges prospects summarized. It expected this review will enable better understand current state field insights into systems

Language: Английский

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An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(15), P. 8831 - 8839

Published: April 4, 2024

Here, we present a method for Salmonella detection using clustered regularly interspaced short palindromic repeats associated with the CRISPR-associated protein 12a-hybridization chain reaction (CRISPR/Cas12a-HCR) system combined polymerase reaction/recombinase-assisted amplification (PCR/RAA) technology. The approach relies on invA gene as biorecognition element and its through PCR RAA. In presence of target gene, Cas12a, guided by crRNA, recognizes cleaves product, initiating HCR. Fluorescently labeled single-stranded DNA (ssDNA) H1 H2 were introduced, concentration was determined based fluorescence intensity from triggered Both assays demonstrate high specificity, sensitivity, simplicity, rapidity. range 2 × 101–2 109 CFU/mL, an LOD 20 entire process enabled specific rapid within 85–105 min. Field-incurred spiked recovery tests conducted in mutton beef samples both assays, demonstrating satisfactory accuracy animal-derived foods. By combining CRISPR/Cas12a hybridization technology, this study presents sensitive that is crucial identifying pathogenic bacteria monitoring food safety.

Language: Английский

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