Development of a Magnetically-Assisted SERS Biosensor for Rapid Bacterial Detection

International Journal of Nanomedicine, Год журнала: 2024, Номер Volume 19, С. 389 - 401

Опубликована: Янв. 1, 2024

Introduction: Ultrasensitive bacterial detection methods are crucial to ensuring accurate diagnosis and effective clinical monitoring, given the significant threat infections pose human health. The aim of this study is develop a biosensor with capabilities for broad-spectrum detection, rapid processing, cost-effectiveness. Methods: A magnetically-assisted SERS was designed, employing wheat germ agglutinin (WGA) recognition antibodies specific capture. Gold nanostars (AuNSs) were sequentially modified Raman reporter molecules WGA, creating versatile tag high affinity diverse range bacteria. Staphylococcus aureus ( S. ) Pseudomonas aeruginosa P. antibody-modified Fe 3 O 4 magnetic gold nanoparticles (MGNPs) served as capture probes. Target bacteria captured by MGNPs combined tags, forming "sandwich" composite structure detection. Results: AuNSs, core size 65 nm, exhibited excellent storage stability (RSD=5.6%) demonstrated superior enhancement compared colloidal nanoparticles. Efficient binding resulted in efficiencies 89.13% 85.31%, respectively. Under optimized conditions, developed assay achieved limit (LOD) 7 CFU/mL 5 . concentration (10– 10 6 CFU/mL) showed strong linear correlation intensity at 1331 cm − 1 Additionally, recoveries (84.8% - 118.0%) low RSD (6.21% 11.42%) observed spiked urine samples. Conclusion: This introduces simple innovative sensitive quantitative or , utilizing WGA antibodies. enhances type biosensor, offering novel platform timely infections. Keywords: SERS, pathogenic bacteria, dual-recognition

Язык: Английский

---

Recent Advances in Lateral Flow Assays for Viral Protein Detection with Nanomaterial-Based Optical Sensors

Biosensors, Год журнала: 2024, Номер 14(4), С. 197 - 197

Опубликована: Апрель 17, 2024

Controlling the progression of contagious diseases is crucial for public health management, emphasizing importance early viral infection diagnosis. In response, lateral flow assays (LFAs) have been successfully utilized in point-of-care (POC) testing, emerging as a viable alternative to more traditional diagnostic methods. Recent advancements virus detection primarily leveraged methods such reverse transcription–polymerase chain reaction (RT-PCR), transcription–loop-mediated isothermal amplification (RT-LAMP), and enzyme-linked immunosorbent assay (ELISA). Despite their proven effectiveness, these conventional techniques are often expensive, require specialized expertise, consume significant amount time. contrast, LFAs utilize nanomaterial-based optical sensing technologies, including colorimetric, fluorescence, surface-enhanced Raman scattering (SERS), offering quick, straightforward analyses with minimal training infrastructure requirements detecting proteins biological samples. This review describes composition mechanism recent protein detection, categorizing them into fluorescent, SERS-based techniques. progress, developing simple, stable, highly sensitive, selective LFA system remains formidable challenge. Nevertheless, an advanced promises not only enhance clinical diagnostics but also extend its utility environmental monitoring beyond, demonstrating potential revolutionize both healthcare safety.

Язык: Английский

---

Interdisciplinary Approach to Monkeypox Prevention: Integrating Nanobiosensors, Nanovaccines, Artificial Intelligence, Visual Arts, and Social Sciences

Small Structures, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

To effectively address crisis emergence of new virus such as monkeypox, a collective and collaborative effort between scientists, engineers, innovators, artists from all ages, regions, diverse fields is required. This review explores holistic approach to addressing the monkeypox by integrating nanobiosensors, artificial intelligence, visual arts, humanities, social sciences. Traditional diagnostic methods are often limited time, accessibility, accuracy, but advancement point‐of‐care smart nanobiosensors offers promising shift toward rapid, precise, accessible diagnostics. They enhance ability screen, diagnose, monitor infections efficiently, contributing better disease management. Beyond technological innovation, essential role sciences in fostering public engagement, understanding, acceptance tools emphasized. Visual arts can illustrate scientific concepts, making them more relatable, while storytelling through various media reduce stigma promote preventive measures. Social provide insights into cultural attitudes, behaviors, health challenges, ensuring that solutions integrated communities. By combining these disciplines, this presents comprehensive framework for resilient global system aligns with One Health principles, emphasizing interconnectedness human, animal, environmental health.

Язык: Английский

---

Highly Sensitive SERS Substrates Based on MoS-Au Nanocomposites for Detection of Hazardous Dyes and Infectious Bacteria

Colloids and Surfaces B Biointerfaces, Год журнала: 2025, Номер unknown, С. 114676 - 114676

Опубликована: Апрель 1, 2025

Язык: Английский

---

Development of a dual-Raman signal lateral flow immunoassay with self-assembling nanogapped SERS tags for the simultaneous monitoring of respiratory bacteria and virus

Sensors and Actuators B Chemical, Год журнала: 2024, Номер 417, С. 136078 - 136078

Опубликована: Июнь 5, 2024

Язык: Английский

---

Sensitive dual-channel lateral flow immunoassay tagged with high brightness latex microsphere for simultaneous detection of respiratory viral antigens

Chemical Engineering Journal, Год журнала: 2024, Номер 497, С. 154826 - 154826

Опубликована: Авг. 13, 2024

Язык: Английский

---

“Cave Effect” Induces Self-Assembled Bimetallic Hollow Structure for Three-in-One Lateral Flow Immunoassay

Nano Letters, Год журнала: 2024, Номер 24(20), С. 5993 - 6001

Опубликована: Апрель 24, 2024

Bimetallic hollow structures have attracted much attention due to their unique properties, but they still face the problems of nonuniform alloys and excessive etching leading structural collapse. Here, uniform bimetallic nanospheres are constructed by pore engineering then highly loaded with hemin (Hemin@MOF). Interestingly, in presence polydopamine (PDA), competitive coordination between anionic polymer (γ-PGA) dimethylimidazole does not lead collapse external framework self-assembly into a structure. By constructing Hemin@MOF immune platform using

Язык: Английский

---

Core–satellite-structured magnetic nanozyme enables the ultrasensitive colorimetric detection of multiple drug residues on lateral flow immunoassay

Analytica Chimica Acta, Год журнала: 2024, Номер 1325, С. 343115 - 343115

Опубликована: Авг. 15, 2024

Язык: Английский

---

Advances and challenges of monkeypox detection technology

Biosafety and Health, Год журнала: 2024, Номер 6(5), С. 260 - 269

Опубликована: Сен. 11, 2024

Язык: Английский

---

“Four-in-One” multifunctional nanohybrids integrated magnetic colorimetric catalytic SERS-driven lateral flow immunoassay for ultrasensitive detection of MPXV

Chemical Engineering Journal, Год журнала: 2024, Номер unknown, С. 155995 - 155995

Опубликована: Сен. 1, 2024

Язык: Английский

---