Real-Time Dynamic Tracking of Multiple Base Excision Repair Enzymes in Living Cells
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 27, 2025
Simultaneous
in
situ
monitoring
of
base
excision
repair
(BER)
correlated
enzymes
like
apurinic/apyrimidinic
endonuclease
1
(APE1)
and
flap
(FEN1)
living
cells
offers
valuable
insights
into
their
roles
disease
development
cytotoxicity
caused
by
pollutants,
but
comprehensive
analysis
is
currently
hindered
diverse
enzyme
functions
limited
methods.
In
this
study,
we
developed
a
dual-activatable
DNA
fluorescent
probe
(AP-FLAP)
to
simultaneously
visualize
APE1
FEN1
activities,
revealing
the
BER-related
damage
various
environmental
pollutants
within
cells.
The
AP-FLAP
was
designed
ingeniously
integrating
dumbbell
structure
containing
5'
hairpin
AP
sites
single
oligonucleotide
probe.
specifically
hydrolyzed
sites,
releasing
5-carboxy-X-rhodamine
(ROX)
signal,
while
recognized
cleaved
flap,
6-carboxyfluorescein
(FAM)
signal.
allowed
for
independent
determination
activities
with
good
specificity
sensitivity.
Subsequently,
applied
investigate
induced
1-methylphenanthrene
(1-MP)
6-chlorobenzo[a]pyrene
(6-Cl-BaP)
human
umbilical
vein
endothelial
(HUVECs).
Significant
1-MP
6-Cl-BaP
exposure
revealed,
positive
correlation
degree
different
concentrations
from
0.1
100
μM.
Notably,
significant
even
at
μM,
concentration-dependent
manner.
Our
work
provides
powerful
tool
elucidating
BER
molecular
mechanisms
under
opens
new
avenues
developing
multifunctional
nucleic
acid
probes
wide
range
applications
chemical
biology
biomedical
research.
Язык: Английский
Effects of POPs-induced SIRT6 alteration on intestinal mucosal barrier function: A comprehensive review
Ecotoxicology and Environmental Safety,
Год журнала:
2025,
Номер
289, С. 117705 - 117705
Опубликована: Янв. 1, 2025
Persistent
organic
pollutants
(POPs)
are
pervasive
chemicals
with
significant
environmental
and
ecological
ramifications,
extending
to
adverse
human
health
effects
due
their
toxicity
persistence.
The
intestinal
mucosal
barrier,
a
sophisticated
defense
mechanism
comprising
the
epithelial
layer,
chemistry,
cellular
immunity,
shields
host
from
external
threats
fosters
symbiotic
relationship
bacteria.
Sirtuin
6
(SIRT6),
sirtuin
family
member,
is
pivotal
in
genome
telomere
stability,
inflammation
regulation,
metabolic
processes.
Result
shows
POPs
have
been
implicated
diseases,
particularly
barrier
dysfunction,
through
mechanisms
such
as
damage,
epigenetic
alterations,
inflammation,
microbiota
changes,
disruptions.
While
impact
of
SIRT6
expression
changes
on
functions
has
reviewed,
linking
remain
elusive.
This
review
summarized
latest
research
results
discussed
role
multiple
perspectives,
proposed
new
directions
POPs,
health,
explored
therapeutic
potential
SIRT6.
Язык: Английский
Genotoxicity and Cytotoxicity Induced In Vitro by Airborne Particulate Matter (PM2.5) from an Open-Cast Coal Mining Area
Atmosphere,
Год журнала:
2024,
Номер
15(12), С. 1420 - 1420
Опубликована: Ноя. 26, 2024
This
study
evaluates
the
cytotoxic
and
genotoxic
effects
of
PM2.5
collected
from
an
open-cast
coal
mining
area
in
northern
Colombia.
Cyclohexane
(CH),
dichloromethane
(DCM),
acetone
(ACE)
extracts
were
obtained
using
Soxhlet
extraction
to
isolate
compounds
different
polarities.
Human
lymphocytes
exposed
extracted
compounds,
cytotoxicity
genotoxicity
assessed
cytokinesis
block
micronucleus
(CBMN)
comet
assays,
incorporating
FPG
ENDO
III
enzymes
detect
oxidative
DNA
damage.
Chemical
analysis
revealed
that
organic
fractions
consisted
mainly
modified
hydrocarbons
volatile
compounds.
The
CBMN
assay
showed
a
significant
increase
micronuclei
binucleated
(MNBN)
mononucleated
(MNMONO)
cells
nucleoplasmic
bridges
(NPB)
lymphocytes.
substantial
damage,
particularly
with
ACE
extract,
which
significantly
increased
oxidized
purines
pyrimidines.
DCM
induced
similar
effects,
while
CH
moderate
effects.
CREST
immunostaining
aneugenic
activity,
extracts.
These
results
suggest
polar
PM2.5,
likely
containing
metals
PAHs,
contribute
damage
chromosomal
instability.
highlights
need
monitor
composition
regions
implement
stricter
environmental
policies
reduce
exposure
health
risks.
Язык: Английский
Association between chronic long-term exposure to airborne dioxins and breast cancer
International Journal of Hygiene and Environmental Health,
Год журнала:
2024,
Номер
263, С. 114489 - 114489
Опубликована: Ноя. 22, 2024
Язык: Английский
Oxidative stress and cellular senescence: Roles in tumor progression and therapeutic opportunities
MedComm – Oncology,
Год журнала:
2024,
Номер
3(4)
Опубликована: Дек. 1, 2024
Abstract
Oxidative
stress
results
from
an
imbalance
between
the
production
and
neutralization
of
reactive
oxygen
species.
It
induces
oxidative
damage
to
cellular
components
including
proteins,
lipids,
nucleic
acids,
membranes,
therefore
intrinsically
linking
aging‐related
diseases
such
as
cancer,
cardiovascular
disease,
neurological
disorders.
Emerging
evidence
suggests
that
may
promote
tumor
development
by
influencing
various
aspects
senescence,
its
onset,
pro‐inflammatory
secretion,
alteration
function
structure.
Modulating
target
senescence
offers
a
novel
strategy
for
cancer
prevention
treatment.
However,
thorough
grasp
specific
mechanisms
at
play
is
lacking.
This
review
will
present
association
their
regulatory
role
in
progression
treatment,
with
emphasis
on
senescence‐associated
secretory
phenotype,
immunosenescence
therapy‐induced
senescence.
Current
agents
strategies
remove
side
effects
via
killing
senescent
cells
or
modulating
improve
antitumor
efficacy
be
summarized.
help
readers
better
understand
complex
relationship
also
provide
basis
further
research
this
area.
Язык: Английский