A metal-trap tests and refines blueprints to engineer cellular protein metalation with different elements
Nature Communications,
Год журнала:
2025,
Номер
16(1)
Опубликована: Янв. 18, 2025
It
has
been
challenging
to
test
how
proteins
acquire
specific
metals
in
cells.
The
speciation
of
metalation
is
thought
depend
on
the
preferences
for
different
competing
at
intracellular
metal-availabilities.
This
implies
mis-metalation
may
occur
if
become
mis-matched
metal-availabilities
heterologous
Here
we
use
a
cyanobacterial
MnII-cupin
(MncA)
as
metal
trap,
predictions
metalation.
By
re-folding
MncA
buffered
metals,
metal-preferences
are
determined.
Relating
estimated
using
cellular
sensors,
predicts
with
FeII
E.
coli.
After
expression
coli,
predominantly
FeII-bound
isolated
experimentally.
predicted
that
metal-supplemented
viable
cells
metal-MncA
should
switch.
MnII-,
CoII-,
or
NiII-MncA
recovered
from
respective
Differences
between
observed
and
used
refine
availabilities.
Values
provided
blueprints
guide
engineering
biological
protein
Язык: Английский
Extrinsic and intrinsic factors affect copper‐induced protein precipitation across eukaryotic and prokaryotic proteomes
Grace R. Sturrock,
Amy T. R. Robison,
Azim Dharani
и другие.
Protein Science,
Год журнала:
2025,
Номер
34(6)
Опубликована: Май 15, 2025
Abstract
The
susceptibility
of
a
protein
to
aggregation
upon
exposure
copper
ions
(Cu)
has
been
recognized
as
contributor
Cu‐induced
cellular
dysfunction
and
toxicity.
Different
cell
types
succumb
Cu
varying
degrees,
indicating
innate
differences
between
species
in
the
mechanisms
used
tolerate
excess
their
biological
needs.
Investigated
here
are
properties
associated
with
metal‐induced
precipitation
(MiPP)
compared
across
lysates
generated
from
three
lines
different
species:
Escherichia
coli
,
Candida
albicans
human
prostate
cancer
line
22Rv1.
was
most
sensitive
precipitation,
while
C.
tolerant.
This
trend
aligns
relative
susceptibilities
these
cells
cytotoxicity.
unique
proteomes
by
were
examined
identify
factors
that
influence
protein's
sensitivity
this
effect.
Identified
intrinsic
such
frequency
solvent
accessibility
known
metal‐binding
amino
acids,
well
external
related
molecular
composition
native
lysates.
Overall,
our
findings
help
elucidate
biomolecular
basis
underpinning
capacity
adventitious
have
differential
effects
on
eukaryotic
prokaryotic
organisms
level
needed
induce
precipitation.
Язык: Английский