Extrinsic and intrinsic factors affect copper‐induced protein precipitation across eukaryotic and prokaryotic proteomes DOI

Grace R. Sturrock,

Amy T. R. Robison,

Azim Dharani

и другие.

Protein Science, Год журнала: 2025, Номер 34(6)

Опубликована: Май 15, 2025

Abstract The susceptibility of a protein to aggregation upon exposure copper ions (Cu) has been recognized as contributor Cu‐induced cellular dysfunction and toxicity. Different cell types succumb Cu varying degrees, indicating innate differences between species in the mechanisms used tolerate excess their biological needs. Investigated here are properties associated with metal‐induced precipitation (MiPP) compared across lysates generated from three lines different species: Escherichia coli , Candida albicans human prostate cancer line 22Rv1. was most sensitive precipitation, while C. tolerant. This trend aligns relative susceptibilities these cells cytotoxicity. unique proteomes by were examined identify factors that influence protein's sensitivity this effect. Identified intrinsic such frequency solvent accessibility known metal‐binding amino acids, well external related molecular composition native lysates. Overall, our findings help elucidate biomolecular basis underpinning capacity adventitious have differential effects on eukaryotic prokaryotic organisms level needed induce precipitation.

Язык: Английский

A metal-trap tests and refines blueprints to engineer cellular protein metalation with different elements DOI Creative Commons
Sophie E. Clough, Tessa R. Young,

Emma Tarrant

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 18, 2025

It has been challenging to test how proteins acquire specific metals in cells. The speciation of metalation is thought depend on the preferences for different competing at intracellular metal-availabilities. This implies mis-metalation may occur if become mis-matched metal-availabilities heterologous Here we use a cyanobacterial MnII-cupin (MncA) as metal trap, predictions metalation. By re-folding MncA buffered metals, metal-preferences are determined. Relating estimated using cellular sensors, predicts with FeII E. coli. After expression coli, predominantly FeII-bound isolated experimentally. predicted that metal-supplemented viable cells metal-MncA should switch. MnII-, CoII-, or NiII-MncA recovered from respective Differences between observed and used refine availabilities. Values provided blueprints guide engineering biological protein

Язык: Английский

Процитировано

2
Extrinsic and intrinsic factors affect copper‐induced protein precipitation across eukaryotic and prokaryotic proteomes DOI

Grace R. Sturrock,

Amy T. R. Robison,

Azim Dharani

и другие.

Protein Science, Год журнала: 2025, Номер 34(6)

Опубликована: Май 15, 2025

Abstract The susceptibility of a protein to aggregation upon exposure copper ions (Cu) has been recognized as contributor Cu‐induced cellular dysfunction and toxicity. Different cell types succumb Cu varying degrees, indicating innate differences between species in the mechanisms used tolerate excess their biological needs. Investigated here are properties associated with metal‐induced precipitation (MiPP) compared across lysates generated from three lines different species: Escherichia coli , Candida albicans human prostate cancer line 22Rv1. was most sensitive precipitation, while C. tolerant. This trend aligns relative susceptibilities these cells cytotoxicity. unique proteomes by were examined identify factors that influence protein's sensitivity this effect. Identified intrinsic such frequency solvent accessibility known metal‐binding amino acids, well external related molecular composition native lysates. Overall, our findings help elucidate biomolecular basis underpinning capacity adventitious have differential effects on eukaryotic prokaryotic organisms level needed induce precipitation.

Язык: Английский

Процитировано

0