A metal-trap tests and refines blueprints to engineer cellular protein metalation with different elements DOI Creative Commons
Sophie E. Clough, Tessa R. Young,

Emma Tarrant

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 18, 2025

It has been challenging to test how proteins acquire specific metals in cells. The speciation of metalation is thought depend on the preferences for different competing at intracellular metal-availabilities. This implies mis-metalation may occur if become mis-matched metal-availabilities heterologous Here we use a cyanobacterial MnII-cupin (MncA) as metal trap, predictions metalation. By re-folding MncA buffered metals, metal-preferences are determined. Relating estimated using cellular sensors, predicts with FeII E. coli. After expression coli, predominantly FeII-bound isolated experimentally. predicted that metal-supplemented viable cells metal-MncA should switch. MnII-, CoII-, or NiII-MncA recovered from respective Differences between observed and used refine availabilities. Values provided blueprints guide engineering biological protein

Language: Английский

A metal-trap tests and refines blueprints to engineer cellular protein metalation with different elements DOI Creative Commons
Sophie E. Clough, Tessa R. Young,

Emma Tarrant

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 18, 2025

It has been challenging to test how proteins acquire specific metals in cells. The speciation of metalation is thought depend on the preferences for different competing at intracellular metal-availabilities. This implies mis-metalation may occur if become mis-matched metal-availabilities heterologous Here we use a cyanobacterial MnII-cupin (MncA) as metal trap, predictions metalation. By re-folding MncA buffered metals, metal-preferences are determined. Relating estimated using cellular sensors, predicts with FeII E. coli. After expression coli, predominantly FeII-bound isolated experimentally. predicted that metal-supplemented viable cells metal-MncA should switch. MnII-, CoII-, or NiII-MncA recovered from respective Differences between observed and used refine availabilities. Values provided blueprints guide engineering biological protein

Language: Английский

Citations

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