Diagnostics,
Год журнала:
2023,
Номер
13(13), С. 2219 - 2219
Опубликована: Июнь 29, 2023
The
emergence
of
COVID-19
has
caused
unprecedented
impacts
on
global
public
health
and
many
other
aspects.
Meanwhile,
types
methods
have
been
developed
to
detect
the
causative
agent,
SARS-CoV-2;
this
greatly
advanced
technologies
in
diagnostic
field.
Here,
we
describe
development
validation
a
sample-in-result-out
POCKIT
Central
SARS-CoV-2
PCR
system
for
detecting
comparison
with
commercial
reference
real-time
RT-PCR
assay
(TaqPath
Combo
Kit).
Both
assays
were
specific
did
not
cross-react
non-SARS-CoV-2
agents.
able
various
strains
including
some
variants.
Based
testing
serial
dilutions
USA-WA1/2020
isolate,
limit
detection
was
0.8
TCID50/mL
(1.87
×
103
genomic
copies/mL)
0.16
(3.75
102
PCR.
Subsequently,
183
clinical
samples
tested
by
both
sensitivity,
specificity,
agreement
91.7%,
100%,
94.0%,
respectively,
when
compared
compact
sample-to-result
is
simplified
efficient
point-of-care
tool
detection.
In
addition,
platform
can
be
readily
adapted
human
animal
viruses.
RSC Advances,
Год журнала:
2023,
Номер
13(26), С. 17883 - 17906
Опубликована: Янв. 1, 2023
Biosensors
based
on
surface
techniques,
combined
with
the
advantage
of
physical/chemical
mechanisms,
demonstrate
great
potential
in
detecting
Covid-19/SARS-CoV-2.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 12, 2025
Cholesteric
liquid
crystal
elastomers
(CLCEs)
possess
a
unique
characteristic
that
allows
the
manipulation
of
reflected
color
by
adjusting
spacing
between
layers.
This
attribute
can
create
reliable
and
cost-effective
colorimetric
sensing
platform
for
on-site
detection
various
substances.
In
this
study,
CLCE
films
were
coupled
with
cross-linked
poly(acrylic
acid)
(CLCE-PAA)
or
poly(dimethylaminoethyl
methacrylate)
(CLCE-PDA)
to
monitor
cholesterol
malathion
levels,
respectively.
both
cases,
change
is
recorded
mobile
phone
camera,
reflectance
wavelength
measured
spectrophotometrically.
For
detection,
smartphone
application
was
used
capture
film's
image,
process
data
into
hue
(H)
values,
calculate
corresponding
concentration
tested
analyte
via
an
analysis
program.
Cholesterol
be
detected
within
linear
range
0.2–1.0
mM
1–10,000
ng/mL,
The
recoveries
actual
sample
86–115%
87–122%
malathion,
system
offers
practical
solution
testing
in
biological
environmental
samples.
Advanced Healthcare Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 7, 2025
In
2024,
mpox
(formerly
"monkeypox")
re-emerged
as
a
global
health
threat
due
to
the
more
severe
Clade
I
strain,
underscoring
need
for
rapid,
accessible
diagnostics.
Traditional
polymerase
chain
reaction
(PCR)
methods,
while
effective,
demand
complex
laboratory
infrastructure
and
extended
processing
times.
To
address
these
limitations,
this
study
presents
fast-flow
micromixer
paper-based
analytical
device
(fmPAD)
designed
ultrasensitive,
rapid
virus
(MPXV)
detection.
The
employs
specially
engineered
sensing
probe
with
cytosine-cytosine
(C─C)
mismatch
sequence
that
hybridizes
MPXV
target,
reactivating
urease
previously
inhibited
by
Ag+
ions
generating
color
change
in
pH-sensitive
indicator.
Enhanced
fluid
mixing
flow
within
fmPAD
allow
efficient
enzymatic
reactivation,
achieving
linear
detection
range
from
1
1000
pm,
limit
of
(LOD)
down
65
fM.
Real
viral
particles
urine
saliva
are
also
successfully
detected
at
concentrations
low
5
copies
mL-1
without
DNA
amplification,
using
portable
colorimeter-representing
lowest
LOD
among
current
sensors.
fmPAD,
combined
readout
technology,
enables
remote,
real-time
analysis,
offering
practical
tool
monitoring
containing
spread
mpox.
Biosensors,
Год журнала:
2025,
Номер
15(5), С. 275 - 275
Опубликована: Апрель 29, 2025
Ecosystem
pollution
by
mercury
ions
(Hg2+)
is
a
major
health
concern,
yet
classical
analytical
methods
for
analysis
are
limited.
This
paper
reviews
the
advances
in
Hg2+
detection
using
DNA
as
recognition
elements
sensors.
molecule
inexpensive,
simple,
and
appropriate
real-time
of
Hg2+.
discusses
DNA-based
sensors
that
were
used
These
can
be
carried
out
electrochemistry,
field
effect
transistors
(FET),
Raman
spectroscopy,
colorimetry,
fluorescence
resonance
energy
transfer
(FRET).
The
principles
advantages
these
also
revealed.
Finally,
provides
an
overview
prospects
potential
challenges
field.
IEEE Sensors Journal,
Год журнала:
2023,
Номер
23(23), С. 28597 - 28604
Опубликована: Окт. 24, 2023
An
ultrasensitive
new
sensor
based
on
functionalized
triangular
gold
nanosheet
layers
for
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
detection
was
proposed.
For
this
purpose,
3-mercaptopropyltrimethoxysilane
(MPTMS)
used
to
fix
nanosheets
(AuTNPs)
the
surface
of
optical
fiber.
AuTNPs
have
a
unique
tip
structure
and
excellent
localized
plasmon
resonance
(LSPR)
effect,
which
can
improve
sensitivity
sensor.
Then,
monoclonal
antibodies
(mAbs)
are
fixed
by
covalent
bonds
make
biosensors.
The
combination
mAb
fiber
through
bond
verified
ultraviolet
(UV)–vis
infrared
characterization.
attached
binds
SARS-CoV-2
antigen
physical
adsorption,
effective
refractive
index
changes,
resulting
in
peak
shift.
At
same
time,
it
is
proved
that
LSPR
shift
not
caused
be
measured.
Au-mAb
biosensor
displays
linear
response
spike
concentration
1.56–312
ng/mL
0.035
nm/(ng/mL).
has
short
time
(10
min),
good
stability,
reused.
broad
application
prospects
medical
diagnostic
field.
