Diagnostics,
Journal Year:
2023,
Volume and Issue:
13(13), P. 2219 - 2219
Published: June 29, 2023
The
emergence
of
COVID-19
has
caused
unprecedented
impacts
on
global
public
health
and
many
other
aspects.
Meanwhile,
types
methods
have
been
developed
to
detect
the
causative
agent,
SARS-CoV-2;
this
greatly
advanced
technologies
in
diagnostic
field.
Here,
we
describe
development
validation
a
sample-in-result-out
POCKIT
Central
SARS-CoV-2
PCR
system
for
detecting
comparison
with
commercial
reference
real-time
RT-PCR
assay
(TaqPath
Combo
Kit).
Both
assays
were
specific
did
not
cross-react
non-SARS-CoV-2
agents.
able
various
strains
including
some
variants.
Based
testing
serial
dilutions
USA-WA1/2020
isolate,
limit
detection
was
0.8
TCID50/mL
(1.87
×
103
genomic
copies/mL)
0.16
(3.75
102
PCR.
Subsequently,
183
clinical
samples
tested
by
both
sensitivity,
specificity,
agreement
91.7%,
100%,
94.0%,
respectively,
when
compared
compact
sample-to-result
is
simplified
efficient
point-of-care
tool
detection.
In
addition,
platform
can
be
readily
adapted
human
animal
viruses.
Advanced Healthcare Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 7, 2025
In
2024,
mpox
(formerly
"monkeypox")
re-emerged
as
a
global
health
threat
due
to
the
more
severe
Clade
I
strain,
underscoring
need
for
rapid,
accessible
diagnostics.
Traditional
polymerase
chain
reaction
(PCR)
methods,
while
effective,
demand
complex
laboratory
infrastructure
and
extended
processing
times.
To
address
these
limitations,
this
study
presents
fast-flow
micromixer
paper-based
analytical
device
(fmPAD)
designed
ultrasensitive,
rapid
virus
(MPXV)
detection.
The
employs
specially
engineered
sensing
probe
with
cytosine-cytosine
(C─C)
mismatch
sequence
that
hybridizes
MPXV
target,
reactivating
urease
previously
inhibited
by
Ag+
ions
generating
color
change
in
pH-sensitive
indicator.
Enhanced
fluid
mixing
flow
within
fmPAD
allow
efficient
enzymatic
reactivation,
achieving
linear
detection
range
from
1
1000
pm,
limit
of
(LOD)
down
65
fM.
Real
viral
particles
urine
saliva
are
also
successfully
detected
at
concentrations
low
5
copies
mL-1
without
DNA
amplification,
using
portable
colorimeter-representing
lowest
LOD
among
current
sensors.
fmPAD,
combined
readout
technology,
enables
remote,
real-time
analysis,
offering
practical
tool
monitoring
containing
spread
mpox.
Biosensors,
Journal Year:
2025,
Volume and Issue:
15(5), P. 275 - 275
Published: April 29, 2025
Ecosystem
pollution
by
mercury
ions
(Hg2+)
is
a
major
health
concern,
yet
classical
analytical
methods
for
analysis
are
limited.
This
paper
reviews
the
advances
in
Hg2+
detection
using
DNA
as
recognition
elements
sensors.
molecule
inexpensive,
simple,
and
appropriate
real-time
of
Hg2+.
discusses
DNA-based
sensors
that
were
used
These
can
be
carried
out
electrochemistry,
field
effect
transistors
(FET),
Raman
spectroscopy,
colorimetry,
fluorescence
resonance
energy
transfer
(FRET).
The
principles
advantages
these
also
revealed.
Finally,
provides
an
overview
prospects
potential
challenges
field.
Biosensors,
Journal Year:
2023,
Volume and Issue:
13(6), P. 640 - 640
Published: June 9, 2023
Viruses
are
widespread
in
the
environment,
and
many
of
them
major
pathogens
serious
plant,
animal,
human
diseases.
The
risk
pathogenicity,
together
with
capacity
for
constant
mutation,
emphasizes
need
measures
to
rapidly
detect
viruses.
highly
sensitive
bioanalytical
methods
diagnose
monitor
socially
significant
viral
diseases
has
increased
past
few
years.
This
is
due,
on
one
hand,
incidence
general
(including
unprecedented
spread
a
new
coronavirus
infection,
SARS-CoV-2),
and,
other
overcome
limitations
modern
biomedical
diagnostic
methods.
Phage
display
technology
antibodies
as
nano-bio-engineered
macromolecules
can
be
used
sensor-based
virus
detection.
review
analyzes
commonly
detection
approaches
shows
prospects
use
prepared
by
phage
sensing
elements
RSC Advances,
Journal Year:
2023,
Volume and Issue:
13(26), P. 17883 - 17906
Published: Jan. 1, 2023
Biosensors
based
on
surface
techniques,
combined
with
the
advantage
of
physical/chemical
mechanisms,
demonstrate
great
potential
in
detecting
Covid-19/SARS-CoV-2.
IEEE Sensors Journal,
Journal Year:
2023,
Volume and Issue:
23(23), P. 28597 - 28604
Published: Oct. 24, 2023
An
ultrasensitive
new
sensor
based
on
functionalized
triangular
gold
nanosheet
layers
for
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2)
detection
was
proposed.
For
this
purpose,
3-mercaptopropyltrimethoxysilane
(MPTMS)
used
to
fix
nanosheets
(AuTNPs)
the
surface
of
optical
fiber.
AuTNPs
have
a
unique
tip
structure
and
excellent
localized
plasmon
resonance
(LSPR)
effect,
which
can
improve
sensitivity
sensor.
Then,
monoclonal
antibodies
(mAbs)
are
fixed
by
covalent
bonds
make
biosensors.
The
combination
mAb
fiber
through
bond
verified
ultraviolet
(UV)–vis
infrared
characterization.
attached
binds
SARS-CoV-2
antigen
physical
adsorption,
effective
refractive
index
changes,
resulting
in
peak
shift.
At
same
time,
it
is
proved
that
LSPR
shift
not
caused
be
measured.
Au-mAb
biosensor
displays
linear
response
spike
concentration
1.56–312
ng/mL
0.035
nm/(ng/mL).
has
short
time
(10
min),
good
stability,
reused.
broad
application
prospects
medical
diagnostic
field.
