Comparative chloroplast genomes provided insights into the evolution and species identification on the Datureae plants DOI Creative Commons
He Su, Xiaoxia Ding, Baosheng Liao

и другие.

Frontiers in Plant Science, Год журнала: 2023, Номер 14

Опубликована: Окт. 24, 2023

Generally, chloroplast genomes of angiosperms are always highly conserved but carry a certain number variation among species. In this study, 13 species from Datureae tribe that importance both in ornamental gardening and medicinal usage were studied. addition, seven together with two Solanaceae retrieved the National Center for Biotechnology Information (NCBI) integrated into study. The ranged size 154,686 to 155,979 155,497 155,919 bp Datura Brugmansia, respectively. As total 128 132 genes identified, which 83 87 protein coding respectively; Furthermore, 37 tRNA 8 rRNA identified Brugmansia. Repeats analysis indicated type varied Simple sequence repeat (SSR), long repeats, tandem repeats 53 59, 98 99, 22 30, Phylogenetic based on plastid supported monophyletic relationship Brugmansia Trompettia, refined phylogenic relationships each individual was resolved. species-specific marker designed spot resulted comparative verified as effective maker identification D. stramonium var. inermis. Interestingly, we found 31 likely be under positive selection, including encoding ATP subunits, photosystem subunit, ribosome NAD(P)H dehydrogenase complex clpP, petB, rbcL, rpoCl, ycf4, cemA genes. These may function key roles adaption diverse environment during evolution. diversification members dated back late Oligocene periods. useful genetic resources taxonomy, phylogeny, evolution Datureae.

Язык: Английский

Cannabis sativa: origin and history, glandular trichome development, and cannabinoid biosynthesis DOI Creative Commons
Ziyan Xie,

Yaolei Mi,

Lingzhe Kong

и другие.

Horticulture Research, Год журнала: 2023, Номер 10(9)

Опубликована: Июль 26, 2023

Is Cannabis a boon or bane? sativa has long been versatile crop for fiber extraction (industrial hemp), traditional Chinese medicine (hemp seeds), and recreational drugs (marijuana). faced global prohibition in the twentieth century because of psychoactive properties ∆9-tetrahydrocannabinol; however, recently, perspective changed with recognition additional therapeutic values, particularly pharmacological potential cannabidiol. A comprehensive understanding underlying mechanism cannabinoid biosynthesis is necessary to cultivate promote globally medicinal application resources. Here, we comprehensively review historical usage Cannabis, trichome-specific cannabinoids, regulatory network trichome development, synthetic biology cannabinoids. This provides valuable insights into efficient green production development utilization novel varieties.

Язык: Английский

Процитировано

23
Lineage‐Specific CYP80 Expansion and Benzylisoquinoline Alkaloid Diversity in Early‐Diverging Eudicots DOI Creative Commons

Zhoujie An,

Ranran Gao, Shanshan Chen

и другие.

Advanced Science, Год журнала: 2024, Номер 11(19)

Опубликована: Март 13, 2024

Abstract Menispermaceae species, as early‐diverging eudicots, can synthesize valuable benzylisoquinoline alkaloids (BIAs) like bisbenzylisoquinoline (bisBIAs) and sinomenines with a wide range of structural diversity. However, the evolutionary mechanisms responsible for their chemo‐diversity are not well understood. Here, chromosome‐level genome assembly Menispermum dauricum is presented demonstrated occurrence two whole duplication (WGD) events that shared by Ranunculales specific to , providing model understanding chromosomal evolution in eudicots. The biosynthetic pathway diverse BIAs M. reconstructed analyzing transcriptome metabolome. Additionally, five catalytic enzymes – one norcoclaurine synthase (NCS) four cytochrome P450 monooxygenases (CYP450s) from formation skeleton, hydroxylated modification, C‐O/C‐C phenol coupling BIAs. Notably, novel leaf‐specific MdCYP80G10 enzyme catalyzes C2′‐C4a ( S )‐reticuline into sinoacutine, enantiomer morphinan compounds, predictable stereospecificity discovered. Moreover, it found ‐specific CYP80 gene expansion, tissue‐specific expression, has driven BIA diversity compared other species. This study sheds light on WGD occurrences eudicots biosynthesis.

Язык: Английский

Процитировано

12
From Species to Varieties: How Modern Sequencing Technologies Are Shaping Medicinal Plant Identification DOI Open Access
Mingcheng Wang,

Haifeng Lin,

Hongqiang Lin

и другие.

Genes, Год журнала: 2024, Номер 16(1), С. 16 - 16

Опубликована: Дек. 26, 2024

Modern sequencing technologies have transformed the identification of medicinal plant species and varieties, overcoming limitations traditional morphological chemical approaches. This review explores key DNA-based techniques, including molecular markers, DNA barcoding, high-throughput sequencing, their contributions to enhancing accuracy reliability identification. Additionally, integration multi-omics approaches is examined provide a comprehensive understanding identity. The literature search for this was conducted across databases such as Google Scholar, Web Science, PubMed, using keywords related taxonomy, genomics, biotechnology. Inclusion criteria focused on peer-reviewed studies closely methods techniques that contribute significantly field. highlights while offer substantial improvements, challenges high costs, technical expertise, lack standardized protocols remain barriers widespread adoption. Potential solutions, AI-driven data analysis portable sequencers, are discussed. provides overview transformative impact, future perspectives more accurate efficient

Язык: Английский

Процитировано

10
Identification of closely related species in Aspergillus through Analysis of Whole-Genome DOI Creative Commons
Guihong Qi,

Lijun Hao,

Yutong Gan

и другие.

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Фев. 21, 2024

The challenge of discriminating closely related species persists, notably within clinical diagnostic laboratories for invasive aspergillosis (IA)-related and food contamination microorganisms with toxin-producing potential. We employed Analysis the whole-GEnome (AGE) to address challenges genus Aspergillus developed a rapid detection method. First, reliable whole genome data 77 were downloaded from database, through bioinformatic analysis, specific targets each identified. Subsequently, sequencing was validate these targets. Additionally, we an on-site method targeting target using editing system. Our results indicate that AGE has successfully achieved identification all IA-related ( fumigatus, niger, nidulans, flavus , terreus ) three well-known A. flavus, parasiticus oryzae section. Flavi have provided species-level-specific . Based on reference targets, substantiate efficacy distinguishing focal its species. Notably, amalgamation room-temperature amplification techniques demonstrates capacity accurate genomic DNA samples at concentration as low 0.1 ng/μl concise 30-min timeframe. Importantly, this methodology circumvents reliance large specialized instrumentation by presenting singular tube operational modality allowing visualized result assessment. These advancements aptly meet exigencies requirements specified species, facilitating prompt diagnosis quality monitoring. Moreover, based species-specific sequences, shows promising potential effective tool epidemiological research classification.

Язык: Английский

Процитировано

7
Phylogenetic Analysis of Rare and Endangered Tulipa Species (Liliaceae) of Kazakhstan Based on Universal Barcoding Markers DOI Creative Commons
Maxim Sutula, А.К. Kakanay, D. Tussipkan

и другие.

Biology, Год журнала: 2024, Номер 13(6), С. 365 - 365

Опубликована: Май 22, 2024

In Kazakhstan, the genus

Язык: Английский

Процитировано

7
Molecular quantification of herbs (Herb-Q): a pyrosequencing-based approach and its application in Pinellia ternata DOI

Mengsha Wei,

Weiguang Sun,

Linlin Liu

и другие.

Chinese Journal of Natural Medicines, Год журнала: 2024, Номер 22(7), С. 663 - 672

Опубликована: Июль 1, 2024

Язык: Английский

Процитировано

5
Comparative and phylogenetic analysis of the chloroplast genomes of four commonly used medicinal cultivars of Chrysanthemums morifolium DOI Creative Commons
Yingying Duan, Yuqing Wang,

Wanyue Ding

и другие.

BMC Plant Biology, Год журнала: 2024, Номер 24(1)

Опубликована: Окт. 22, 2024

Язык: Английский

Процитировано

5
Metabolomics and transcriptomics reveal the mechanism of alkaloid synthesis in Corydalis yanhusuo bulbs DOI Creative Commons
Xiao Zhao, Yuan Pan, Jun Tan

и другие.

PLoS ONE, Год журнала: 2024, Номер 19(5), С. e0304258 - e0304258

Опубликована: Май 23, 2024

Corydalis yanhusuo W.T. Wang is a traditional herb. Benzylisoquinoline alkaloids (BIAs) are the main pharmacological active ingredients that play an important role in sedation, relieving pain, promoting blood circulation, and inhibiting cancer cells. However, there few studies on biosynthetic pathway of benzylisoquinoline , especially some specific components, such as tetrahydropalmatine. We carried out widely targeted metabolome transcriptomic analyses to construct identified candidate genes. In this study, 702 metabolites were detected, including 216 alkaloids. Protoberberine-type aporphine-type chemical components C . bulbs. Key genes for biosynthesis, 6-OMT CNMT NMCH BBE SOMT1 CFS SPS STOX MSH TNMT P6H successfully identified. There was no significant difference content expression level between two suborgans (mother-bulb son-bulb). The levels BIA expansion stage (MB-A SB-A) significantly higher than those maturity (MB-C SB-C), consistent with pattern gene regulation. Five complete single likely encode functional enzyme CoOMT, which participated tetrahydropalmatine biosynthesis These provide strong theoretical basis subsequent development metabolic engineering (especially tetrahydropalmatine)

Язык: Английский

Процитировано

4
Natural products as drug leads: exploring their potential in drug discovery and development DOI
Kuldeep Singh, Jeetendra Kumar Gupta, Dilip Kumar Chanchal

и другие.

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер unknown

Опубликована: Дек. 2, 2024

Язык: Английский

Процитировано

4
Development of a mitochondrial mini-barcode and its application in metabarcoding for identification of leech in traditional Chinese medicine DOI Creative Commons

Chenfeng Shi,

Yuhan Guo,

Lijuan Yao

и другие.

Scientific Reports, Год журнала: 2025, Номер 15(1)

Опубликована: Янв. 11, 2025

Abstract In Traditional Chinese Medicine (TCM), the medicinal leech is vital for treatments to promote blood circulation and eliminate stasis. However, prevalence of counterfeit products in market undermines quality efficacy these remedies. DNA barcoding techniques, such as COI barcode, have been limited their application due amplification challenges. This study identified high variability 16 S rRNA gene within mitochondrial genome across five species, leading development a novel 219 bp mini-barcode. Compared with traditional our mini-barcode showed remarkable identification efficiency, classifying 142 out 147 samples from fresh processed materials. contrast, barcode could only successfully identify 79 samples. case seven batches decoction pieces, six, whereas recognized one. Additionally, effectively discerned species patent medicines when combined metabarcoding technology. These results confirm mini-barcode’s potential reliable tool rapidly precisely identifying TCM products.

Язык: Английский

Процитировано

0