Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 24, 2023
Generally,
chloroplast
genomes
of
angiosperms
are
always
highly
conserved
but
carry
a
certain
number
variation
among
species.
In
this
study,
13
species
from
Datureae
tribe
that
importance
both
in
ornamental
gardening
and
medicinal
usage
were
studied.
addition,
seven
together
with
two
Solanaceae
retrieved
the
National
Center
for
Biotechnology
Information
(NCBI)
integrated
into
study.
The
ranged
size
154,686
to
155,979
155,497
155,919
bp
Datura
Brugmansia,
respectively.
As
total
128
132
genes
identified,
which
83
87
protein
coding
respectively;
Furthermore,
37
tRNA
8
rRNA
identified
Brugmansia.
Repeats
analysis
indicated
type
varied
Simple
sequence
repeat
(SSR),
long
repeats,
tandem
repeats
53
59,
98
99,
22
30,
Phylogenetic
based
on
plastid
supported
monophyletic
relationship
Brugmansia
Trompettia,
refined
phylogenic
relationships
each
individual
was
resolved.
species-specific
marker
designed
spot
resulted
comparative
verified
as
effective
maker
identification
D.
stramonium
var.
inermis.
Interestingly,
we
found
31
likely
be
under
positive
selection,
including
encoding
ATP
subunits,
photosystem
subunit,
ribosome
NAD(P)H
dehydrogenase
complex
clpP,
petB,
rbcL,
rpoCl,
ycf4,
cemA
genes.
These
may
function
key
roles
adaption
diverse
environment
during
evolution.
diversification
members
dated
back
late
Oligocene
periods.
useful
genetic
resources
taxonomy,
phylogeny,
evolution
Datureae.
Foods,
Год журнала:
2025,
Номер
14(4), С. 608 - 608
Опубликована: Фев. 12, 2025
As
a
key
component
of
both
traditional
medicine
and
modern
healthcare,
Food–Medicine
Homologous
Herbal
Materials
have
attracted
considerable
attention
in
recent
years.
However,
issues
related
to
the
quality
authenticity
medicinal
materials
on
market
often
arise,
not
only
compromising
their
efficacy
but
also
presenting
potential
risks
consumer
health.
Therefore,
establishment
accurate
efficient
identification
methods
is
crucial
for
ensuring
safety
Food-Medicine
Materials.
This
paper
provides
systematic
review
research
progress
Materials,
starting
with
such
as
morphological
microscopic
identification,
focusing
applications
techniques,
including
biomimetic
recognition,
chromatography,
mass
spectrometry,
chromatography–mass
spectrometry
coupling,
hyperspectral
imaging,
near-infrared
spectroscopy,
terahertz
DNA
barcoding.
Moreover,
it
comprehensive
analysis
fundamental
principles,
advantages,
limitations
these
methods.
Finally,
outlines
current
challenges
faced
by
suggests
future
directions
improvement,
aiming
offer
technical
perspective
identifying
foster
further
development
this
field.
Phytochemical Analysis,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 4, 2025
Despite
significant
advances
in
analytical
methods,
the
quality
evaluation
and
adulteration
detection
of
chemically
complex
traditional
Chinese
medicines
continues
to
be
a
major
challenge.
We
developed
custom
diffusion-ordered
spectroscopy
(DOSY)
approach
for
qualitative
analysis
precious
Calculus
Bovis
(CB)
its
adulterants.
Using
Artificial
as
an
example,
we
focused
on
effects
NMR
spectrometers,
solvent
viscosity,
matrices,
matrix
concentration,
temperature
separation
efficiency
DOSY.
The
optimal
conditions
also
successfully
applied
identification
Natural
Bovis,
Sativus,
adulterated
samples.
accuracy
DOSY
was
verified
by
13C
NMR.
Via
inspection
solvents,
temperature,
experiments
CB
were
found
using
methanol-d4
solvent,
adding
4
mg
polyvinyl
pyrrolidone
(PVP)
matrix,
298
K.
Under
these
conditions,
five
types
compounds,
cholic
acid
(CA),
deoxycholic
(DCA),
hyodeoxycholic
(HDCA),
taurine-conjugated
bile
acids
(tBAs),
glycine-conjugated
(gBAs),
unequivocally
identified
three
CB.
validated
results.
As
sugar-adulterated
CB,
signals
sugar
could
clearly
spectrum.
This
study
feasibility
provided
reference
point
future
quantitative
other
medicines,
well
characterization
Toxics,
Год журнала:
2025,
Номер
13(4), С. 247 - 247
Опубликована: Март 26, 2025
The
European
Union
Drugs
Agency
has
emphasized
the
increasing
difficulty
in
monitoring
drug
market
due
to
emergence
of
new
psychoactive
substances,
often
marketed
as
legal
highs.
proliferation
fake
pharmacies,
drugstores,
and
e-commerce
platforms
made
access
illicit
substances
alarmingly
rapid
inexpensive.
These
are
readily
available
without
medical
prescriptions,
lacking
proper
risk
assessments
or
potential
adverse
effects,
raising
significant
public
health
concerns.
Today,
relentless
pursuit
validation
success-often,
at
any
cost-has
led
an
exponential
rise
use
cognitive
mood
enhancers.
Such
frequently
consumed
manage
demands
related
work,
diet,
sexuality,
sleep,
achievement,
interpersonal
relationships.
Consequently,
investigating
these
phenomena
is
critically
important
for
institutions,
they
represent
a
serious
threat
individual
development
health.
Developing
effective
preventive
protective
systems
essential.
This
review
provides
overview
currently
smart
drugs,
discussing
their
desired
neuropharmacological
psychological
implications,
decline
resulting
from
excessive
unregulated
use.
concludes
that
multidisciplinary
approach
combining
molecular
identification,
micro-morphological
analysis,
chemical
characterization
crucial
accurate
detection,
monitoring,
mitigation
substances.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(5), С. 2955 - 2955
Опубликована: Март 3, 2024
Survival
crises
stalk
many
animals,
especially
endangered
and
rare
animals.
Accurate
species
identification
plays
a
pivotal
role
in
animal
resource
conservation.
In
this
study,
we
developed
an
method
called
Analysis
of
whole-GEnome
(AGE),
which
identifies
by
finding
species-specific
sequences
through
bioinformatics
analysis
the
whole
genome
subsequently
recognizing
these
using
experimental
technologies.
To
clearly
demonstrate
AGE
method,
Cervus
nippon,
well-known
species,
closely
related
elaphus,
were
set
as
model
without
with
published
genomes,
respectively.
By
analyzing
genomes
C.
nippon
obtained
next-generation
sequencing
online
databases,
built
specific
sequence
databases
containing
7,670,140
570,981
sequences,
Then,
specificities
confirmed
experimentally
Sanger
CRISPR-Cas12a
system.
Moreover,
for
11
fresh
samples
35
commercially
available
products,
our
results
complete
agreement
those
other
authoritative
methods,
demonstrating
AGE’s
precision
potential
application.
Notably,
found
mixture
products
successfully
identified
it.
This
study
broadens
horizons
sheds
light
on
conserving
resources.
