Comparative chloroplast genomes provided insights into the evolution and species identification on the Datureae plants DOI Creative Commons
He Su, Xiaoxia Ding, Baosheng Liao

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 24, 2023

Generally, chloroplast genomes of angiosperms are always highly conserved but carry a certain number variation among species. In this study, 13 species from Datureae tribe that importance both in ornamental gardening and medicinal usage were studied. addition, seven together with two Solanaceae retrieved the National Center for Biotechnology Information (NCBI) integrated into study. The ranged size 154,686 to 155,979 155,497 155,919 bp Datura Brugmansia, respectively. As total 128 132 genes identified, which 83 87 protein coding respectively; Furthermore, 37 tRNA 8 rRNA identified Brugmansia. Repeats analysis indicated type varied Simple sequence repeat (SSR), long repeats, tandem repeats 53 59, 98 99, 22 30, Phylogenetic based on plastid supported monophyletic relationship Brugmansia Trompettia, refined phylogenic relationships each individual was resolved. species-specific marker designed spot resulted comparative verified as effective maker identification D. stramonium var. inermis. Interestingly, we found 31 likely be under positive selection, including encoding ATP subunits, photosystem subunit, ribosome NAD(P)H dehydrogenase complex clpP, petB, rbcL, rpoCl, ycf4, cemA genes. These may function key roles adaption diverse environment during evolution. diversification members dated back late Oligocene periods. useful genetic resources taxonomy, phylogeny, evolution Datureae.

Language: Английский

DOSY‐Assisted Multicomponent Characterization Enables Classification of Precious Chinese Materia Medica and Identification of Adulteration: A Case Study of Calculus Bovis DOI

S.J. Li,

Fang Cuifen,

Sinan Wang

et al.

Phytochemical Analysis, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Despite significant advances in analytical methods, the quality evaluation and adulteration detection of chemically complex traditional Chinese medicines continues to be a major challenge. We developed custom diffusion-ordered spectroscopy (DOSY) approach for qualitative analysis precious Calculus Bovis (CB) its adulterants. Using Artificial as an example, we focused on effects NMR spectrometers, solvent viscosity, matrices, matrix concentration, temperature separation efficiency DOSY. The optimal conditions also successfully applied identification Natural Bovis, Sativus, adulterated samples. accuracy DOSY was verified by 13C NMR. Via inspection solvents, temperature, experiments CB were found using methanol-d4 solvent, adding 4 mg polyvinyl pyrrolidone (PVP) matrix, 298 K. Under these conditions, five types compounds, cholic acid (CA), deoxycholic (DCA), hyodeoxycholic (HDCA), taurine-conjugated bile acids (tBAs), glycine-conjugated (gBAs), unequivocally identified three CB. validated results. As sugar-adulterated CB, signals sugar could clearly spectrum. This study feasibility provided reference point future quantitative other medicines, well characterization

Language: Английский

Citations

0
Fluorescent signal-based molecular method for identification and quantification of adulteration in Panax ginseng from raw materials to processed products DOI
Wenjun Jiang, Yifei Pei, Mingyu Zhang

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106426 - 106426

Published: March 1, 2025

Language: Английский

Citations

0
The Dark Side of “Smart Drugs”: Cognitive Enhancement vs. Clinical Concerns DOI Creative Commons

Mariarosaria Ingegneri,

Erika Smeriglio,

Younes Zebbiche

et al.

Toxics, Journal Year: 2025, Volume and Issue: 13(4), P. 247 - 247

Published: March 26, 2025

The European Union Drugs Agency has emphasized the increasing difficulty in monitoring drug market due to emergence of new psychoactive substances, often marketed as legal highs. proliferation fake pharmacies, drugstores, and e-commerce platforms made access illicit substances alarmingly rapid inexpensive. These are readily available without medical prescriptions, lacking proper risk assessments or potential adverse effects, raising significant public health concerns. Today, relentless pursuit validation success-often, at any cost-has led an exponential rise use cognitive mood enhancers. Such frequently consumed manage demands related work, diet, sexuality, sleep, achievement, interpersonal relationships. Consequently, investigating these phenomena is critically important for institutions, they represent a serious threat individual development health. Developing effective preventive protective systems essential. This review provides overview currently smart drugs, discussing their desired neuropharmacological psychological implications, decline resulting from excessive unregulated use. concludes that multidisciplinary approach combining molecular identification, micro-morphological analysis, chemical characterization crucial accurate detection, monitoring, mitigation substances.

Language: Английский

Citations

0
Identification of toxic Gelsemium elegans in processed food and honey based on real-time PCR analysis DOI
Gang Wang, Ying Ren, Yuying Su

et al.

Food Research International, Journal Year: 2024, Volume and Issue: 182, P. 114188 - 114188

Published: March 1, 2024

Language: Английский

Citations

3
Analysis of Whole-Genome as a Novel Strategy for Animal Species Identification DOI Open Access

Yutong Gan,

Guihong Qi,

Lijun Hao

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2955 - 2955

Published: March 3, 2024

Survival crises stalk many animals, especially endangered and rare animals. Accurate species identification plays a pivotal role in animal resource conservation. In this study, we developed an method called Analysis of whole-GEnome (AGE), which identifies by finding species-specific sequences through bioinformatics analysis the whole genome subsequently recognizing these using experimental technologies. To clearly demonstrate AGE method, Cervus nippon, well-known species, closely related elaphus, were set as model without with published genomes, respectively. By analyzing genomes C. nippon obtained next-generation sequencing online databases, built specific sequence databases containing 7,670,140 570,981 sequences, Then, specificities confirmed experimentally Sanger CRISPR-Cas12a system. Moreover, for 11 fresh samples 35 commercially available products, our results complete agreement those other authoritative methods, demonstrating AGE’s precision potential application. Notably, found mixture products successfully identified it. This study broadens horizons sheds light on conserving resources.

Language: Английский

Citations

3
Adulteration detection of Gastrodia elata Blume and its commercial products using chloroplast DNA marker-based species-specific PCR assays DOI
Xiaofei Shi, Qingfeng Zhang, Hongtao Wang

et al.

Microchemical Journal, Journal Year: 2024, Volume and Issue: 201, P. 110513 - 110513

Published: April 10, 2024

Language: Английский

Citations

3
Analysis of Whole-Genome for Identification of Seven Penicillium Species with Significant Economic Value DOI Open Access
Y. H. Huang,

Lianguo Fu,

Yutong Gan

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(15), P. 8172 - 8172

Published: July 26, 2024

The

Language: Английский

Citations

3
Use of a Novel Combination of Multiplex PCR and DNA Barcoding in Assessing Authenticity of Ginseng Products DOI Creative Commons
Diane Y. Kim,

Donna Miranda-Romo,

Adriana R. Ten Cate

et al.

Food Control, Journal Year: 2024, Volume and Issue: unknown, P. 110893 - 110893

Published: Sept. 1, 2024

Language: Английский

Citations

3
Natural products as drug leads: exploring their potential in drug discovery and development DOI
Kuldeep Singh, Jeetendra Kumar Gupta, Dilip Kumar Chanchal

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 2, 2024

Language: Английский

Citations

3
Advances in the Identification Methods of Food-Medicine Homologous Herbal Materials DOI Creative Commons
Yuying Jiang,

Shilei Wei,

Hongyi Ge

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(4), P. 608 - 608

Published: Feb. 12, 2025

As a key component of both traditional medicine and modern healthcare, Food–Medicine Homologous Herbal Materials have attracted considerable attention in recent years. However, issues related to the quality authenticity medicinal materials on market often arise, not only compromising their efficacy but also presenting potential risks consumer health. Therefore, establishment accurate efficient identification methods is crucial for ensuring safety Food-Medicine Materials. This paper provides systematic review research progress Materials, starting with such as morphological microscopic identification, focusing applications techniques, including biomimetic recognition, chromatography, mass spectrometry, chromatography–mass spectrometry coupling, hyperspectral imaging, near-infrared spectroscopy, terahertz DNA barcoding. Moreover, it comprehensive analysis fundamental principles, advantages, limitations these methods. Finally, outlines current challenges faced by suggests future directions improvement, aiming offer technical perspective identifying foster further development this field.

Language: Английский

Citations

0