Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 24, 2023
Generally,
chloroplast
genomes
of
angiosperms
are
always
highly
conserved
but
carry
a
certain
number
variation
among
species.
In
this
study,
13
species
from
Datureae
tribe
that
importance
both
in
ornamental
gardening
and
medicinal
usage
were
studied.
addition,
seven
together
with
two
Solanaceae
retrieved
the
National
Center
for
Biotechnology
Information
(NCBI)
integrated
into
study.
The
ranged
size
154,686
to
155,979
155,497
155,919
bp
Datura
Brugmansia,
respectively.
As
total
128
132
genes
identified,
which
83
87
protein
coding
respectively;
Furthermore,
37
tRNA
8
rRNA
identified
Brugmansia.
Repeats
analysis
indicated
type
varied
Simple
sequence
repeat
(SSR),
long
repeats,
tandem
repeats
53
59,
98
99,
22
30,
Phylogenetic
based
on
plastid
supported
monophyletic
relationship
Brugmansia
Trompettia,
refined
phylogenic
relationships
each
individual
was
resolved.
species-specific
marker
designed
spot
resulted
comparative
verified
as
effective
maker
identification
D.
stramonium
var.
inermis.
Interestingly,
we
found
31
likely
be
under
positive
selection,
including
encoding
ATP
subunits,
photosystem
subunit,
ribosome
NAD(P)H
dehydrogenase
complex
clpP,
petB,
rbcL,
rpoCl,
ycf4,
cemA
genes.
These
may
function
key
roles
adaption
diverse
environment
during
evolution.
diversification
members
dated
back
late
Oligocene
periods.
useful
genetic
resources
taxonomy,
phylogeny,
evolution
Datureae.
Phytochemical Analysis,
Год журнала:
2024,
Номер
35(4), С. 664 - 677
Опубликована: Янв. 15, 2024
Abstract
Introduction
The
COVID‐19
pandemic
was
associated
with
an
increased
global
use
of
traditional
medicines,
including
Ayurvedic
herbal
preparations.
Due
to
their
growing
demand,
processed
nature,
and
the
complexity
supply
chain,
there
is
risk
adulteration
in
these
products.
Objectives
objective
this
study
assess
DNA
barcoding
for
species
identification
supplements
on
US
market
treatment
respiratory
symptoms.
Methods
A
total
54
commercial
products
containing
herbs
were
tested
four
regions
(i.e.,
rbcL
,
matK
ITS2,
mini‐ITS2)
using
two
composite
samples
per
product.
Nine
categories
targeted:
amla,
ashwagandha,
cinnamon,
ginger,
guduchi,
tribulus,
tulsi,
turmeric,
vacha.
Results
At
least
one
identified
64.8%
expected
detected
38.9%
Undeclared
plant
species,
other
herbs,
rice,
pepper,
19
products,
fungal
12
presence
undeclared
may
be
a
result
intentional
substitution
or
contamination
during
harvest
processing,
while
likely
material
environment.
greatest
sequencing
success
(42.6–46.3%)
obtained
primers.
Conclusion
results
indicate
that
combination
genetic
loci
should
used
supplements.
limitations
future
research
incorporate
chemical
characterization
techniques.
Results in Chemistry,
Год журнала:
2024,
Номер
7, С. 101344 - 101344
Опубликована: Янв. 1, 2024
The
stem
bark
of
Salix
alba
(SA)
is
extensively
used
in
herbal
medicine
and
nutraceuticals.
non-availability
quality
standard
available
for
SA
the
Ayurvedic
Pharmacopeia
India
(API)
leads
inability
to
trace
adulteration.
In
current
study,
has
been
developed
interest
control,
as
per
international
standards
including
API
parameters.
geographical
changes
could
play
a
vital
role
phytochemicals’
nutrients’
variations.
Hence,
samples
from
different
geolocations
markets
were
analysed
along
with
cultivated
plant
sample.
A
comprehensive
study
identification
authentication
by
DNA
barcoding
pharmacognostic
evaluation,
physicochemical
phytochemical
analysis
HPTLC
was
carried
out.
Moreover,
validated
HPLC
method
quantification
three
phytochemicals
(Salicin,
Procyanidin
B1
Catechin)
standardization
bark.
complete
profiling
volatile
metabolites
non-volatile
done
using
GC–MS
LC-MS
techniques,
respectively.
ICP-OES
based
elemental
analysis,
heavy
metals
observed
below
permissible
limits,
shown
significant
amount
essential
metal
ions
like
Na,
Mg,
Ca,
Fe,
Cu,
Zn.
total
phenol
content
(TPC)
flavonoid
(TFC)
all
ranged
16.69
50.30
mg
GAE/
g
wt.
124.57
347.76
QCE/
wt.,
DPPH
assay,
IC50
values
0.58
2.08
mg/mL.
antibacterial
promising
results
against
S.
aureus
B.
subtilis
strains
inhibition
zones
20
±
4
mm.
Overall,
this
first
report
comprising
advanced
detailed
important
other
vitro
assays.
revealed
that
fresh
sample
may
contain
activity.
Frontiers in Plant Science,
Год журнала:
2023,
Номер
14
Опубликована: Окт. 24, 2023
Generally,
chloroplast
genomes
of
angiosperms
are
always
highly
conserved
but
carry
a
certain
number
variation
among
species.
In
this
study,
13
species
from
Datureae
tribe
that
importance
both
in
ornamental
gardening
and
medicinal
usage
were
studied.
addition,
seven
together
with
two
Solanaceae
retrieved
the
National
Center
for
Biotechnology
Information
(NCBI)
integrated
into
study.
The
ranged
size
154,686
to
155,979
155,497
155,919
bp
Datura
Brugmansia,
respectively.
As
total
128
132
genes
identified,
which
83
87
protein
coding
respectively;
Furthermore,
37
tRNA
8
rRNA
identified
Brugmansia.
Repeats
analysis
indicated
type
varied
Simple
sequence
repeat
(SSR),
long
repeats,
tandem
repeats
53
59,
98
99,
22
30,
Phylogenetic
based
on
plastid
supported
monophyletic
relationship
Brugmansia
Trompettia,
refined
phylogenic
relationships
each
individual
was
resolved.
species-specific
marker
designed
spot
resulted
comparative
verified
as
effective
maker
identification
D.
stramonium
var.
inermis.
Interestingly,
we
found
31
likely
be
under
positive
selection,
including
encoding
ATP
subunits,
photosystem
subunit,
ribosome
NAD(P)H
dehydrogenase
complex
clpP,
petB,
rbcL,
rpoCl,
ycf4,
cemA
genes.
These
may
function
key
roles
adaption
diverse
environment
during
evolution.
diversification
members
dated
back
late
Oligocene
periods.
useful
genetic
resources
taxonomy,
phylogeny,
evolution
Datureae.
