Polygenetic Analysis of Colchicum Species Using 18S rRNA Gene Sequence DOI Open Access
Ezgi Çabuk Şahin

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

Colchicum has played a vital role in the field of medicine due to its inherent properties, with importance extending from past present. DNA barcoding is an effective tool for identification and conservation plant species. This study tested application 18S rRNA gene phylogenetic construction The UPGMA (unweighted pair group method arithmetic mean) tree STRUCTURE analysis were conducted assess consistency results 33 specimens one outgroup species, utilizing partial gene. showed that using was identifying 97.76% sequences being successfully determined. contributes species by illustrating utility construction, underscoring significance advancing our understanding genetics. nucleotide obtained this uploaded barcode life data system (BOLD), contributing internationally accessible genetic data.

Язык: Английский

Chemoprofiling and medicinal potential of underutilized leaves of Cyperus scariosus DOI Creative Commons
Yashika Gandhi, Vijay Kumar, Gagandeep Singh

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Март 27, 2024

Abstract Agro-waste is the outcome of under-utilization bioresources and a lack knowledge to re-use this waste in proper ways or circular economy approach. In Indian medicinal system, root Cyperus scariosus (CS) used at large scale due their vital properties. Unfortunately, aerial part CS treated as agro-waste an under-utilized bioresource. Due knowledge, weed. This present study first ever attempt explore leaves medicinally nutrient rich source. To determine food nutritional values neglected R.Br. (CS), i.e. leaves, phytochemicals metal ions were quantified by newly developed HPLC ICPOES-based methods. The content observed analysis for caffeic acid, catechin, epicatechin, trans-p-coumaric trans-ferulic acid was 10.51, 276.15, 279.09, 70.53, 36.83 µg/g, respectively. GC–MS/MS analysis, fatty acids including linolenic phytol, palmitic etc. identified. ICPOES significant Na, K, Ca, Cu, Fe, Mg, Mn, Zn observed. TPC TFC 17.933 mg GAE eq./g 130.767 QCE along with IC 50 value 2.78 mg/mL DPPH assay better antacid activity measured than standard (CaCO 3 ). methanolic extract showed anti-microbial against Staphylococcus aureus (15 ± 2 mm), Pseudomonas aeruginosa (12 mm) Escherichia coli (10 mm). silico studies confirmed vitro results obtained from antioxidant, antiacid, studies. addition, revealed anti-cancerous anti-inflammatory potential leaves. study, thus, demonstrated significance conversion into mankind pharmaceutical potent material. Consequently, highlighted that have importance good utility industry improving bio-valorization environment.

Язык: Английский

Процитировано

7

Simultaneously quantification of eight marker compounds by HPLC, and HPTLC analysis for the marker-based shelf-life kinetic study for the standardization of polyherbal drug AYUSH SG-5, medicinal properties and computational studies DOI
Yashika Gandhi,

Vikram Kushwaha,

Vijay Kumar

и другие.

Microchemical Journal, Год журнала: 2024, Номер 199, С. 109958 - 109958

Опубликована: Янв. 10, 2024

Язык: Английский

Процитировано

3

A Comparative Analysis of Phytochemicals, Metal Ions, Volatile Metabolites in Heart Wood, Stem Bark and Leaves of Salix alba L. along with in Vitro Antioxidant, Antacid, Antimicrobial Activities for Sake of Environment Conservation by Substitution of Stem Bark With Leaf DOI
Yashika Gandhi, Hemant Rawat, Daljeet Singh Dhanjal

и другие.

Chemistry & Biodiversity, Год журнала: 2023, Номер 20(12)

Опубликована: Окт. 23, 2023

The genus of Salix is used in food, medicine and nutraceuticals, standardized by using the single marker compound Salicin only. Stem bark official part for preparation various drugs, nutraceuticals food products, which may lead to overexploitation damage tree. There need search substitution stem with leaf alba L. (SA), yet not reported. Comparative phytochemicals viz. Salicin, Procyanidin B1 Catechin were quantified parts SA heart wood (SA-HW), (SA-SB) leaves (SA-L) L.by newly developed HPLC method. It was observed that SA-HW SA-L contained far better amount B as compared SA-SB (SA-HW~SA-L≫SA-SB). Essential toxic metal ions all three analysed ICP-OES method, where founded a rich source micronutrients essential SA-HW. GC-MS analysis has shown presence fatty acids volatile compounds. TPC TFC values ranged from 2.69 32.30 mg GAE/g wt. 37.57 220.76 QCE/g respectively. In DPPH assay IC50 SA-SB, SA-HW, 1.09 (±0.02), 5.42 (±0.08), 8.82 (±0.10) mg/mL, order antibacterial activities against E. coli, S. aureus, P. aeruginosa, B. subtilis strains SA-L>SA-HW>SA-SB strong strains. antacid SA-L>SA-SB>SA-HW. have significant nutrients, medicinal properties than SA-SB. be considered substitute save environment or avoid over exploitation, but after complete pharmacological toxicological studies.

Язык: Английский

Процитировано

4

Effects of geographical variation on the phytochemicals gallic acid, corilagin, and ellagic acid, as well as medicinal properties of Emblica officinalis Gaertn (Fruit) DOI
Yashika Gandhi, Sujeet K. Mishra, Vijay Kumar

и другие.

Food and Humanity, Год журнала: 2024, Номер 3, С. 100372 - 100372

Опубликована: Авг. 8, 2024

Язык: Английский

Процитировано

1

A Review on the Traditional Applications, Phytochemistry, and Pharmacology of the Genus Physochlaina G. Don DOI
Younas Rasheed Tantray, Ishrat Jan, Irshad Mohiuddin

и другие.

Chemistry & Biodiversity, Год журнала: 2024, Номер 21(6)

Опубликована: Март 26, 2024

Physochlaina is a genus of flowering plants belonging to the family Solanaceae and consists 10 species distributed in various Asian countries. The have been traditionally used cure variety illnesses due their highly valuable medicinal properties, including cancer, asthma, cough, weakness, stomachache, diarrhea, swelling, spasms, toothache, boils, ulcers, rheumatic pain, chronic bronchitis, gastric problems, abdominal palpitation, insomnia. gained significant attention remarkable ethnopharmacological ethnomedicinal significance. researchers isolated so far 71 biologically active secondary metabolites from different species, which include flavonoids, alkaloids, coumarins, phenolic acids, iridoids, sterols. These compounds exhibit diverse biological activities, such as antibacterial, anti-oxidant, anti-inflammatory, cytotoxic, anticancer properties. present review has compiled with intention providing comprehensive overview botany, distribution, traditional uses, phytochemical profile, activities for future exploration plant-based drugs therapeutic approaches. contributes understanding pharmacological potential unraveling chemical composition, highlighting relevance developing agents. Till date, numerous properties phytochemicals that support ethnobotanical history documented number scientific publications. However, greater emphasis should be paid vivo investigations on extracts phytoconstituents well mechanistic analysis help drug developers better understand how use resources herbal formulations using techniques.

Язык: Английский

Процитировано

0

Analytical method development and validation for simultaneous estimation of seven markers in polyherbal formulation JKC by using RP-HPLC DOI Creative Commons
Lakshminarayana Misro, Thirupataiah Boini, Rahul Kumar Maurya

и другие.

Future Journal of Pharmaceutical Sciences, Год журнала: 2024, Номер 10(1)

Опубликована: Июль 29, 2024

Abstract Background The RP-HPLC method has been established to simultaneous estimation of seven markers in polyherbal formulation JKC using the C 18 (25 × 0.46 cm, i.d,5 µm) column. mobile phase consisted methanol: water (80:20) at a flow rate 1.0 mL/min and observed retention time 2 11 min with sharp points. marker compounds viz. Andrographolide (AG), Piperine (PP), Picroside-I (P-I), Picroside-II (P-II), α-Cyprone (AC), 6-Shogaol (6S), 6-Gingerol (6G) were quantified formulations by HPLC method. Detection was performed wavelength (λ) 229 nm for AG, 343 PP, 279 P-I, 264 P-II, 254 AC, 280 both 6S 6G HPLC–PDA detector. Results different times (R t ) i.e. AG 3.060 ± 0.01 min, PP 5.460 0.03 P-I 2.789 0.02 P-II 2.553 AC 10.951 6.302 4.111 respectively. proposed validated acceptable linearity (r 0.9995–0.9999), precision, robustness, ruggedness, accuracy (RSD < 2%) under optimum conditions. limit detection quantification bioactive as: (1.386; 4.200 ppm), (2.033; 6.161 (2.822; 8.553 (2.538; 7.691 (0.269; 0.815 (0.158; 0.480 (0.188; 0.569 ppm). amount (mg/g) detected estimated plants (41.282 0.48; 10.06 0.18), (53.81 0.25, 13.82 0.37 PN, PL; 4.27 0.07), (15.97 0.01; 0.48 0.003), (63.24 0.35; 2.31 0.006), (0.42 0.36 (0.71 0.03; 0.16 0.001), (2.64 0.09; 0.12 0.004) Method found be rugged robust. results all validation parameters within limits according ICH guidelines. Conclusion is fast, precise, economic, specific used simultaneously quantifiable analysis major ingredients (herbs) formulations.

Язык: Английский

Процитировано

0

Polygenetic Analysis of Colchicum Species Using 18S rRNA Gene Sequence DOI Open Access
Ezgi Çabuk Şahin

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, Год журнала: 2024, Номер unknown

Опубликована: Окт. 12, 2024

Colchicum has played a vital role in the field of medicine due to its inherent properties, with importance extending from past present. DNA barcoding is an effective tool for identification and conservation plant species. This study tested application 18S rRNA gene phylogenetic construction The UPGMA (unweighted pair group method arithmetic mean) tree STRUCTURE analysis were conducted assess consistency results 33 specimens one outgroup species, utilizing partial gene. showed that using was identifying 97.76% sequences being successfully determined. contributes species by illustrating utility construction, underscoring significance advancing our understanding genetics. nucleotide obtained this uploaded barcode life data system (BOLD), contributing internationally accessible genetic data.

Язык: Английский

Процитировано

0