Thermotolerance and plasticity of camel somatic cells exposed to acute and chronic heat stress

Journal of Advanced Research, Год журнала: 2019, Номер 22, С. 105 - 118

Опубликована: Ноя. 22, 2019

The Arabian camel is the largest known mammal that can survive in severe hot climatic conditions. We provide molecular explanation for thermotolerance of granulosa somatic cells after exposure to 45 °C 2 (acute heat shock) or 20 h (chronic shock). common features cellular responses acute stress were increase shock proteins and DNA repair enzymes expression. Actin polymerization Rho signaling critically activated as a defense against shock. Cells exposed chronic showed altered cell architecture with decrease total detected proteins, metabolic enzymes, cytoskeletal protein Treatment transforming growth factor beta (TGFβ) pathway inhibitor SB-431542 suppressed morphological alterations Moreover, during recovery stage at 38 24 h, proteomic changes partially restored an exponential HSP70 expression, their normal morphology on 9th day recovery. Full proteomics data are available via ProteomeXchange identifier PXD012159. strategies tolerance both thermal conditions reflect flexible adaptability conserve life under extremely

Язык: Английский

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Transcriptome Reveals Granulosa Cells Coping through Redox, Inflammatory and Metabolic Mechanisms under Acute Heat Stress

Cells, Год журнала: 2022, Номер 11(9), С. 1443 - 1443

Опубликована: Апрель 25, 2022

Heat stress affects granulosa cells (GCs) and the ovarian follicular microenvironment, causing poor oocyte developmental competence fertility. This study aimed to investigate physical responses global transcriptomic changes in bovine GCs acute heat (43 °C for 2 h) vitro. Heat-stressed exhibited transient proliferation senescence resumed at 48 h post-stress, while post-stress immediate culture-media change had a relatively positive effect on resumption. Increased accumulation of reactive oxygen species apoptosis was observed heat-stress group. In spite upregulation inflammatory (CYCS, TLR2, TLR4, IL6, etc.), pro-apoptotic (BAD, BAX, TNFSF9, MAP3K7, TNFRSF6B, FADD, TRADD, RIPK3, etc.) caspase executioner genes (CASP3, CASP8, CASP9), antioxidants anti-apoptotic (HMOX1, NOS2, CAT, SOD, BCL2L1, GPX4, were also upregulated heat-stressed GCs. Progesterone estrogen hormones, along with steroidogenic gene expression, declined significantly, involved cholesterol synthesis. Out 12,385 differentially expressed (DEGs), 330 significant DEGs (75 upregulated, 225 downregulated) subjected KEGG functional pathway annotation, ontology enrichment, STRING network analyses manual querying meaningful molecular mechanisms. High response found be responsible oxidative-stress-mediated nodes towards involvement NF-κB repression Nrf2 pathway. Downregulation MDM4, TP53, PIDD1, PARP3, MAPK14 MYC, STK26, STK33, TGFB2, CDKN1A CDKN2A, interface MAPK p53 signaling pathway, can attributed cellular The background working AMPK through AKT1, AMPK, SIRT1, PYGM, SLC2A4 SERBP1 genes, downregulation PPARGCIA, IGF2, PPARA, SLC27A3, SLC16A3, TSC1/2, KCNJ2, KCNJ16, etc., evidence transcriptional activity energetic homeostasis modifications stress. presents detailed acute-heat-stressed physical, levels interesting insights into future studies regarding GC adaptation their interaction oocytes reproductive system level.

Язык: Английский

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Cellular and Molecular Adaptation of Arabian Camel to Heat Stress

Frontiers in Genetics, Год журнала: 2019, Номер 10

Опубликована: Июнь 19, 2019

To cope with the extreme heat stress and drought of desert, Arabian camel (Camelus dromedarius) has developed exceptional physiological biochemical particularities. Previous reports focused mainly on features neglected its cellular molecular characteristics. Heat shock proteins are suggested to play a key role in protein homeostasis thermotolerance. Therefore, we aim by this review elucidate implication HSPs adaptation compare them related mammalian species. Correlation these molecules adaptive mechanisms is special importance expand our understanding overall physiology homeostasis.

Язык: Английский

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Dromedary camel: A model of heat resistant livestock animal

Theriogenology, Год журнала: 2020, Номер 154, С. 203 - 211

Опубликована: Июнь 1, 2020

Язык: Английский

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Methionine Modulates the Growth and Development of Heat-Stressed Dermal Papilla Cells via the Wnt/β-Catenin Signaling Pathway

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(4), С. 1495 - 1495

Опубликована: Фев. 11, 2025

This study furnishes insights into how methionine mitigates heat-stress-induced impairments in hair follicle development Rex rabbits at the cellular level. Dermal papilla cells from dorsal skin of were isolated, cultured vitro, and divided six groups, i.e., control (37 °C; 0 mM methionine), heat stress (45 + 15 mM, 30 45 60 with replicates per group. The groups exposed to °C, 5% CO2, 95% humidity for min, followed by recovery 37 repeated three times over days. On third day, samples collected post-heat stress. results show that markedly fortified HSP70, MSRA, SOD expression (p < 0.01); augmented proliferation ameliorated cell cycle progression; lessened apoptosis 0.05). Adding Wnt signaling pathway activators inhibitors manifested these effects associated diminished β-catenin phosphorylation aggrandized Wnt10b, 0.001), LEF/TCF nuclear transcription factors 0.01). Thus, this demonstrates regulates growth heat-stressed via pathway, remitting heat-stress trauma.

Язык: Английский

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Investigation of Metabolome Underlying the Biological Mechanisms of Acute Heat Stressed Granulosa Cells

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(4), С. 2146 - 2146

Опубликована: Фев. 15, 2022

Heat stress affects granulosa cells and the ovarian follicular microenvironment, ultimately resulting in poor oocyte developmental competence. This study aims to investigate metabo-lomics response of bovine (bGCs) vitro acute heat 43 °C. triggers oxidative stress-mediated apoptosis cultured bGCs. Heat-stressed bGCs exhibited a time-dependent recovery proliferation potential by 48 h. A total 119 metabolites were identified through LC-MS/MS-based metabolomics spent culture media, out which, 37 determined as differentially involved metabolic pathways related bioenergetics support mechanisms physical adaptations Multiple analyses metabolome data choline, citric acid, 3-hydroxy-3-methylglutaric glutamine, glycocyamine being upregulated, while galactosamine, AICAR, ciliatine, 16-hydroxyhexadecanoic lysine, succinic uridine, xanthine, uraconic acid important downregulated stress. These differential implicated various directed towards including glycerophospholipid metabolism, citrate cycle (TCA cycle), glyoxylate dicarboxylate serine, threonine, tyrosine metabolism. Our presents adaptation vitro.

Язык: Английский

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Cellular and functional adaptation to thermal stress in ovarian granulosa cells in mammals

Journal of Thermal Biology, Год журнала: 2020, Номер 92, С. 102688 - 102688

Опубликована: Авг. 1, 2020

Язык: Английский

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Transcriptomics Reveals Granulosa Cell’s Coping through Redox, Inflammatory, Metabolic and Cytoskeleton Mechanisms under Acute Heat Stress

Опубликована: Фев. 28, 2022

Heat stress affects the granulosa cells (GCs) and ovarian follicular microenvironment, causing poor oocyte developmental competence fertility. This study aimed to investigate physical responses global transcriptomic changes in bovine GCs acute heat (43 ℃ for 2 h) in-vitro gave essential insights into general interaction at cell&ndash;stress nexus. Heat-stressed exhibited transient proliferation senescence, resumed 48 h post-stress. While post-stress immediate culture-media change had a relatively positive effect on resumption. Increased accumulation of reactive oxygen species apoptosis was observed group. In spite upregulation pro-apoptotic caspase executioner genes, antioxidants anti-apoptotic genes were also upregulated heat-stressed GCs. Progesterone Estrogen hormones along with steroidogenic expression, declined significantly, involved cholesterol synthesis. Out 12385 differentially expressed (DEGs), 330 significant DEGs (75 upregulated, 225 downregulated) subjected KEGG functional pathway annotation, gene ontology enrichment, STRING network analyses. Based manual query DEGs, enrichment analyses, vast interplay among all major signaling pathways strongly evidence repression cellular transcriptional activity, averting effects through remodeling structural proteins energetic-homeostasis. presents detailed physical, transcriptional, levels interesting future studies regarding adaptation their reproductive system level.

Язык: Английский

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Autophagy is a pro-survival adaptive response to heat shock in bovine cumulus-oocyte complexes

Scientific Reports, Год журнала: 2020, Номер 10(1)

Опубликована: Авг. 13, 2020

Abstract Autophagy is a physiological mechanism that can be activated under stress conditions. However, the role of autophagy during oocyte maturation has been poorly investigated. Therefore, this study characterized on developmental competence and gene expression bovine oocytes exposed to heat shock (HS). Cumulus-oocyte-complexes (COCs) were matured at Control (38.5 °C) HS (41 temperatures in presence 0 10 mM 3-methyladenine (3MA; inhibitor). Western blotting analysis revealed increased marker LC3-II/LC3-I ratio oocytes. there was no effect temperature for with 3MA. On cumulus cells, 3MA reduced regardless temperature. Inhibition IVM heat-shocked (3MA-41 cleavage blastocyst rates compared standard vitro (IVM-41 °C). magnitude detrimental effects greater inhibitor. Oocyte 3MA-41 °C mRNA abundance genes related energy metabolism ( MTIF3 ), response HSF1 HAS2 GREM1 ). In conclusion, induced shocked modulated key functional processes rendering more susceptible deleterious shock.

Язык: Английский

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Molecular characterization of alpha subunit 1 of sodium pump (ATP1A1) gene in Camelus dromedarius: its differential tissue expression potentially interprets the role in osmoregulation

Molecular Biology Reports, Год журнала: 2022, Номер 49(5), С. 3849 - 3861

Опубликована: Март 2, 2022

Язык: Английский

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