Intermittent mild cold acclimation ameliorates intestinal inflammation and immune dysfunction in acute cold-stressed broilers by regulating the TLR4/MyD88/NF-κB pathway

Poultry Science, Год журнала: 2024, Номер 103(5), С. 103637 - 103637

Опубликована: Март 8, 2024

To investigate the potential protective effect of prior cold stimulation on broiler intestine induced by acute stress (ACS). A total 384 one-day-old broilers were divided into control (CON), ACS, Ⅰ (CS3+ACS), and Ⅱ (CS9+ACS) groups. Broilers in CON ACS groups reared normally, birds CS3+ACS CS9+ACS at 3℃ 9℃ below group for 5 h, respectively, alternate days from day 15 to 35. CS3+ACS, subjected 10℃ 24 h 43. Eventually, small tissues collected histopathological observation indexes detection. The results showed that intestinal all ACS-broilers exhibited inflammatory cell infiltrates, microvilli disruption, reduced villus length jejunum increased crypt depth ileum. Whereas these phenomena relatively light group. Compared group, mRNA expression TLR4/MyD88/NF-κB pathway-related genes (TLR4, MyD88, NF-κBp65, COX-2, iNOS, PTGEs, TNF-α), Th1/Th17-derived cytokines (IL-1β, IL-2, IL-8, IL-12, IFN-γ, IL-17), HSPs (HSP40, HSP60, HSP70, HSP90) was upregulated (P < 0.05), Th2-deviated (IL-4, IL-6, IL-10, IL-13) IκBα downregulated 0.05) almost ACS-broilers. most genes, cytokines, Th2-derived 0.05). Protein levels TLR4, p-p65/p65, p-IκBα/IκBα, IKK, TNF-α, IL-1β, similar their expression. concentration sIgA activities CAT, SOD, GSH-px decreased MDA H2O2 compared Therefore, caused oxidative inflammation, leading gut immune dysfunction; while mild normal rearing temperature alleviated stress-induced injure dysfunction modulating pathway broilers.

Язык: Английский

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New insights into Microalgal astaxanthin's effect on Lambda-cyhalothrin-induced lymphocytes immunotoxicity in Cyprinus carpio: Involving miRNA-194-5p-FoxO1-mediated-mitophagy and pyroptosis

Fish & Shellfish Immunology, Год журнала: 2023, Номер 141, С. 109046 - 109046

Опубликована: Сен. 1, 2023

Язык: Английский

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Resveratrol improves emamectin benzoate-induced pyroptosis and inflammation of Ctenopharyngodon idellus hepatic cells by alleviating oxidative stress/endoplasmic reticulum stress

Fish & Shellfish Immunology, Год журнала: 2023, Номер 142, С. 109148 - 109148

Опубликована: Окт. 6, 2023

Язык: Английский

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Effects of intermittent cold stimulation on growth performance, meat quality, antioxidant capacity and liver lipid metabolism in broiler chickens

Poultry Science, Год журнала: 2024, Номер 103(3), С. 103442 - 103442

Опубликована: Янв. 9, 2024

Intermittent cold stimulation (ICS) enhances broilers' resistance to stress. Nonetheless, further research is needed investigate the underlying mechanisms that enhance stress resistance. A total of 160 one-day-old male Ross 308 broilers were randomly divided into two groups (CC and CS5), with CC group managing temperature according standard for broiler growth stages, while CS5 subjected at a 3℃ lower than 5 h, every days from 15-35 day. Sampling was conducted 36 day (36D), 50 (50D) after acute 24 h (Y24). First, we examined effects ICS on performance, meat quality, antioxidant capacity, lipid metabolism. The results demonstrated enhanced performance certain degree. Specifically, average weight gain in significantly higher group, feed conversion ratio decreased compared 4 W 6 (P ≤ 0.05). Compared reduced drip loss, shearing force, yellowness (a* value) chicken meat, increased redness (b* At Y24, levels T-AOC GSH-PX serum those level MDA content TG, FFA, VLDL elevated, whereas TC HDL In addition, explored whether AMPK-mTOR pathway involved regulation changes metabolism possible regulatory downstream signaling pathway. showed upregulated expression AMPK mRNA protein liver 36D down-regulating mTOR down-regulated synthesis endoplasmic reticulum stress-related genes (SREBP1c, FAS, SCD, ACC, GRP78 PERK) up-regulating decomposition autophagy-related (PPAR LC3) (GRP78) lower, (LC3 ATG7) can affect quality broilers, when are stress, trained have stronger capacity.

Язык: Английский

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Long-term music stimulating alleviated the inflammatory responses caused by acute noise stress on the immune organs of broilers by NF-κB signaling pathway

Ecotoxicology and Environmental Safety, Год журнала: 2024, Номер 273, С. 116131 - 116131

Опубликована: Фев. 26, 2024

As an environmental enrichment, music can positively influence the immune function, while noise has adverse effect on physical and mental health of humans animals. However, whether music-enriched environments mitigate noise-induced acute stress remains unclear. To investigate anti-inflammatory effects organs broiler chickens under conditions early-life stress, 140 one-day-old white feather broilers (AA) were randomly divided into four groups: control (C), stimulation (M) group, (N) followed by (NM) group. At 14 days age, N NM groups received 120 dB for 10 min one week. After stimulation, group M subjected to continuous (6 h per day, 60 dB). 28 body temperature chicks, histopathological changes, quantification ROS-positive density apoptosis positivity in tissues spleen, thymus, bursa Fabricius (BF) measured. The results showed that led increase number area splenic microsomes cortex/medulla ratio detected organs. activities superoxide dismutase (SOD) glutathione peroxidase (GSH-Px) decreased compared C mRNA levels malondialdehyde (MDA), TNF-α, IL-1, IL-1β increased. In addition, gene protein expression IKK, NF-κB, IFN-γ three from Compared a decrease follicles, SOD GSH-Px, MDA, IL-1β. Therefore, environment attenuate oxidative induced inhibiting activation NF-κB signaling pathway consequently alleviating inflammatory response

Язык: Английский

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Baicalein inhibits apoptosis and autophagy induced by chlorpyrifos exposure to kidney of Cyprinus carpio through activation of PI3K/AKT pathway

Pesticide Biochemistry and Physiology, Год журнала: 2023, Номер 196, С. 105624 - 105624

Опубликована: Сен. 16, 2023

Язык: Английский

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Dietary resveratrol supplementation alleviates cold exposure-induced pyroptosis and inflammation in broiler heart by modulating oxidative stress and endoplasmic reticulum stress

Poultry Science, Год журнала: 2024, Номер 103(11), С. 104203 - 104203

Опубликована: Авг. 10, 2024

To explore the potential protective effect of resveratrol (RES) on cold-exposed broilers, 360 21-day-old broilers were equally divided into 5 groups with 6 replicates. A control (CON) group was reared at normal feeding temperature and received a basal diet, 4 cold exposure (8 ± 1°C for 10 h/d from d 29 to 42) fed diet 0 (CE), 250 (CE + RES250), 500 RES500), 750 RES750) mg/kg RES 22 42. Broilers slaughtered 42 heart tissues collected measure relevant indexes. The results showed that all CE-broilers had inflammatory cell infiltrations, dietary supplementation reduced this phenomenon. Compared CON group, concentrations MDA H

Язык: Английский

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“Unveiling the impacts of climatic cold events on the cardiovascular health in animal models”

The Science of The Total Environment, Год журнала: 2025, Номер 971, С. 179028 - 179028

Опубликована: Март 1, 2025

Язык: Английский

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Effects of Dietary Terminalia chebula Extract on Growth Performance, Immune Function, Antioxidant Capacity, and Intestinal Health of Broilers

Animals, Год журнала: 2024, Номер 14(5), С. 746 - 746

Опубликована: Фев. 28, 2024

Terminalia chebula extract (TCE) has many physiological functions and is potentially helpful in maintaining poultry health, but its specific effect on the growth of broilers not yet known. This research investigated effects dietary supplementation performance, immune function, antioxidant capacity, intestinal health yellow-feathered broilers. A total 288 one-day-old were divided into four treatment groups (72 broilers/group), each with six replicates 12 The given a basal diet corn–soybean meal supplemented 0 (control), 200, 400, 600 mg/kg TCE for 56 d. results demonstrated that, compared diet, addition significantly increased (linear quadratic, p < 0.05) final body weight overall gain performance decreased feed-to-gain ratio period. Dietary (linear, levels IgM, IL-4, IL-10 level IL-6 serum. IL-2 IL-1β, liver. IgM IL-10, IgG, IL-1β spleen. Supplementation linearly quadratically (p catalase, superoxide dismutase, glutathione peroxidase, capacity activities while decreasing malonic dialdehyde concentrations serum, liver, TCE-containing diets resulted higher villus height, height to crypt depth, lower depth diet. acetic butyric acid (quadratic, isovaleric concentration. Bacteroidaceae Bacteroides, which regulate richness diversity microorganisms, more abundant contained when was added In conclusion, these findings demonstrate that supplementing could boost their gut improving performance; they also provide reference future TCE.

Язык: Английский

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Exploring the sex dimorphism in the expression of intestinal barrier and immune-related genes and intestinal microbiota in cage-cultured large yellow croaker (Larimichthys crocea) during the overwintering period along the Zhoushan coast

Frontiers in Marine Science, Год журнала: 2024, Номер 11

Опубликована: Апрель 4, 2024

In Zhejiang province, large yellow croakers are primarily cultured in net cages, facing significant challenges during the overwintering period such as susceptibility to cold and starvation stress. Notably, observable sexual dimorphism croaker hints at likelihood of gender differences their responses these environmental stresses. However, potential sex-specific adaptive changes remain unexplored. To gain deeper insights, we investigated expression intestinal barrier-related genes, immune responses, microbiota males females separately. The results revealed a more pronounced loss body weight than that males. male intestines, there was decrease genes (arp2/3, occludin, zo1), contrasting with increase females. TLR1, TLR3, TLR7, TLR9, MyD88, NF-κB intestines female fish decreased significantly March compared November, while opposite trend observed fish. liver, were both inflammatory cytokine (IL-1β IL-6). females, IL-1β gene decreased, IL-6 increased significantly. IL-10 exhibited genes. Further analysis greater diversity period. Firmicutes’ relative abundance opposing between Proteobacteria abundance, driven by Vibrio bacteria, conclusion, may compromise structural integrity reducing function. Additionally, response strategy differs sexes. findings provide crucial insights for crafting effective strategies management decisions cage-cultured period, well offering theoretical references monosex aquaculture.

Язык: Английский

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