Application of lacrimal gland ultrasonography in the evaluation of chronic ocular graft-versus-host-disease DOI Creative Commons
Min Zhong, Siyuan Liu,

Jiangping Luo

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 5, 2025

Objective To investigate the effectiveness of lacrimal gland ultrasonography in assessment chronic ocular graft-versus-host-disease (oGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and to establish correlation between surface ultrasonographic results. Method The cross-sectional study included 57 participants aged 18 older, who were at least 100 days allo-HSCT. was conducted oGVHD clinic Peking University People’s Hospital March June 2023. Patients categorized into groups according International Chronic (ICCGVHD) consensus group diagnostic criteria or 2005 National Institutes Health (NIH) classification for GVHD. Demographics transplantation-related information collected all participants, including age, gender, donor-recipient HLA matching, ABO gender combination duration disease activity severity involvement assessed using various parameters such as Ocular Surface Disease Index (OSDI), Schirmer test, tear film break-up time (BUT), meniscus height, corneal/conjunctival staining meibomian dropout. Lacrimal structures by B-mode Doppler measure long diameter, thick homogeneity parenchymal vascularization. Statistical analyses performed determine differences conditions well condition findings. Result (1) with definite probable exhibited a significantly longer allo-HSCT compared non-oGVHD patients (H=11.264, p<0.01), median durations 247(164,894) 525(310,928) days, respectively, 204(169,323.25) patients. (2) Compared patients, both showed lower average test (H=31.188, TBUT (H=11.853, height (H=13.630, p<0.01) higher OSDI (F=27.992, corneal scores (χ²=23.66, p<0.05) temporal conjunctival (χ²=14.84, p<0.05). (3) glands vascularization did not exhibit significant three groups. (4) diameter had positive correlations (r=0.297, negative (r=-0.313, total (r=-0.285, demonstrated (r=0.404, (r=-0.273, p<0.05), (r=-0.264, nasal (r=-0.271, (r=-0.312, Homogeneity found be correlated status. Conclusion is worse than that observed non-GVHD main manifestations include keratoconjunctival injury reduction secretion stability. These effects appear common result chemoradiotherapy-induced inflammation rejection-associated responses. There no morphology revealed ultrasonography. This suggests rejection may primary cause changes While can provide insight secretion, its efficacy diagnosing appears limited.

Язык: Английский

PINK1/Parkin-Mediated Mitophagy Ameliorates Mitochondrial Dysfunction in Lacrimal Gland Acinar Cells During Aging DOI Creative Commons
Han Zhao, Yue Zhang, Yujie Ren

и другие.

Investigative Ophthalmology & Visual Science, Год журнала: 2024, Номер 65(13), С. 12 - 12

Опубликована: Ноя. 6, 2024

Purpose: Aging alters the function of lacrimal gland and disrupts balance microenvironment on ocular surface, eventually leading to aqueous-tear-deficient dry eye. Mitophagy has been reported play an important role in aging, but underlying mechanism remains unclear. Methods: The young (6 weeks) middle-aged (12 months) male C57BL/6J mice were used this study, mitophagy agonist rapamycin inhibitor Mdivi-1 vivo experiments. Hematoxylin eosin, Masson, Oil Red O, reactive oxygen species (ROS) staining detect histological changes lipids gland. Changes expression proteins identified by Western blotting lysates. Transmission electron microscopy immunofluorescence assess mitophagy. single-cell RNA sequencing (scRNA-seq) bioinformatics analyses transcription signature during aging. Results: In we discovered that aging increased oxidative stress, which apoptosis, generated ROS acinar epithelial cells. Furthermore, activation PINK1/Parkin-mediated reduced concentrations prevented aging-induced apoptosis cells, thereby causing alterations, microstructural degradation, increasing tear secretion associated with accumulation. By contrast, aggregates mitochondrial thereafter leads impairment inhibiting fission giving rise Conclusions: Overall, our findings suggested could impair age-related alterations may be treated therapeutic approaches enhance while maintaining function.

Язык: Английский

Процитировано

4
Application of lacrimal gland ultrasonography in the evaluation of chronic ocular graft-versus-host-disease DOI Creative Commons
Min Zhong, Siyuan Liu,

Jiangping Luo

и другие.

Frontiers in Immunology, Год журнала: 2025, Номер 16

Опубликована: Фев. 5, 2025

Objective To investigate the effectiveness of lacrimal gland ultrasonography in assessment chronic ocular graft-versus-host-disease (oGVHD) after allogeneic hematopoietic stem cell transplantation (allo-HSCT) and to establish correlation between surface ultrasonographic results. Method The cross-sectional study included 57 participants aged 18 older, who were at least 100 days allo-HSCT. was conducted oGVHD clinic Peking University People’s Hospital March June 2023. Patients categorized into groups according International Chronic (ICCGVHD) consensus group diagnostic criteria or 2005 National Institutes Health (NIH) classification for GVHD. Demographics transplantation-related information collected all participants, including age, gender, donor-recipient HLA matching, ABO gender combination duration disease activity severity involvement assessed using various parameters such as Ocular Surface Disease Index (OSDI), Schirmer test, tear film break-up time (BUT), meniscus height, corneal/conjunctival staining meibomian dropout. Lacrimal structures by B-mode Doppler measure long diameter, thick homogeneity parenchymal vascularization. Statistical analyses performed determine differences conditions well condition findings. Result (1) with definite probable exhibited a significantly longer allo-HSCT compared non-oGVHD patients (H=11.264, p<0.01), median durations 247(164,894) 525(310,928) days, respectively, 204(169,323.25) patients. (2) Compared patients, both showed lower average test (H=31.188, TBUT (H=11.853, height (H=13.630, p<0.01) higher OSDI (F=27.992, corneal scores (χ²=23.66, p<0.05) temporal conjunctival (χ²=14.84, p<0.05). (3) glands vascularization did not exhibit significant three groups. (4) diameter had positive correlations (r=0.297, negative (r=-0.313, total (r=-0.285, demonstrated (r=0.404, (r=-0.273, p<0.05), (r=-0.264, nasal (r=-0.271, (r=-0.312, Homogeneity found be correlated status. Conclusion is worse than that observed non-GVHD main manifestations include keratoconjunctival injury reduction secretion stability. These effects appear common result chemoradiotherapy-induced inflammation rejection-associated responses. There no morphology revealed ultrasonography. This suggests rejection may primary cause changes While can provide insight secretion, its efficacy diagnosing appears limited.

Язык: Английский

Процитировано

0