Preparation of a miR-155-activating nucleic acid nanoflower to study the molecular mechanism of miR-155 in inflammation DOI Creative Commons
Wenxin Wang,

Jie Geng,

Xiaohan Wu

и другие.

Molecular Medicine, Год журнала: 2022, Номер 28(1)

Опубликована: Июнь 17, 2022

At present, the molecular mechanisms underlying inflammation remain unclear. In recent years, research on has focused stimulating cell by using exogenous pro-inflammatory substances such as lipopolysaccharide (LPS) or inflammatory factors. To investigate mechanism of from a new perspective, we designed nucleic acid nanoflowers (NFs) complex to directly activate genes study response without need for external microbial factors trigger an response. An RNAa-type target gene-activated NFs was designed. Human umbilical vein endothelial cells (HUVECs) were transfected with carrying small activating RNA (saRNAs) co-activate microRNA (miR)-155 and SHIP1 genes. After activation (RNAa)-type transferred into HUVECs, expression miR-155 cancer-related increased, anti-inflammatory reduced, proliferation migration promoted. IL-1β protein levels decreased downregulated. When its both activated, unaltered, maintaining homeostasis. This points towards overexpression can inflammation, that act switch role in development inflammation.

Язык: Английский

The Effect of Akkermansia muciniphila and Its Outer Membrane Vesicles on MicroRNAs Expression of Inflammatory and Anti-inflammatory Pathways in Human Dendritic Cells DOI

Laya Zoghi Mofrad,

Abolfazl Fateh, Fattah Sotoodehnejadnematalahi

и другие.

Probiotics and Antimicrobial Proteins, Год журнала: 2023, Номер 16(2), С. 367 - 382

Опубликована: Март 8, 2023

Язык: Английский

Процитировано

6
Immunomodulatory Effects of Canine Adipose Tissue Mesenchymal Stem Cell-Derived Extracellular Vesicles on Stimulated CD4+ T Cells Isolated from Peripheral Blood Mononuclear Cells DOI Creative Commons
Takahiro Teshima, Yunosuke Yuchi, Ryôhei Suzuki

и другие.

Journal of Immunology Research, Год журнала: 2021, Номер 2021, С. 1 - 20

Опубликована: Авг. 14, 2021

Adipose tissue-derived mesenchymal stem cells (ADSCs) have anti-inflammatory and immunomodulatory characteristics. Many studies suggested that the immunomodulation of ADSCs is largely mediated by secreted paracrine factors. Various factors are from ADSCs, among which extracellular vesicles considered to play a major role in communication between target cells. Several reported function canine ADSC-derived (cADSC-EVs), but few effects cADSC-EVs on immune The purpose this study was investigate vitro-stimulated CD4+ T isolated peripheral blood mononuclear (PBMCs). were cADSCs under naive conditions or primed tumor necrosis factor-α (TNFα) interferon-γ (IFNγ). expression levels several microRNAs altered priming with TNFα IFNγ. Culturing PBMCs stimulated concanavalin A presence inhibited differentiation promoted apoptosis PBMCs. CD4+, CD8+, CD4+CD8+ decreased, while CD3+CD4-CD8- increased. helper (Th) 1, Th2, Th17, regulatory (Treg) analyzed flow cytometry. proliferation Th1 Th17 enhanced Th2 Treg cell proliferation. However, had incorporated labeled comprised only percent all Therefore, these responses may be due not direct also indirect through interactions other In conclusion, exert immunosuppressive vitro. These findings useful for further diseases.

Язык: Английский

Процитировано

14
The association of plasma levels of miR-146a, miR-27a, miR-34a, and miR-149 with coronary artery disease DOI

Sara Hosseinpor,

Bahman Khalvati, Farshad Safari

и другие.

Molecular Biology Reports, Год журнала: 2022, Номер 49(5), С. 3559 - 3567

Опубликована: Май 1, 2022

Язык: Английский

Процитировано

9
Aberrant expression and regulatory role of histone deacetylase 9 in vascular endothelial cell injury in intracranial aneurysm DOI Creative Commons
Jingwei Sun,

Langfeng Zhang,

Quanjiang Cheng

и другие.

Biomolecules and Biomedicine, Год журнала: 2023, Номер unknown

Опубликована: Авг. 12, 2023

Intracranial aneurysm (IA) is one of the most challenging cerebrovascular lesions for clinicians. The aim this study was to investigate abnormal expression and role histone deacetylase 9 (HDAC9) in IA-associated injury vascular endothelial cells (VECs). First, IA tissue normal arterial were collected VECs isolated from patients. levels HDAC9, microRNA (miR)-34a-5p, growth factor-A (VEGFA) determined. Cell viability, proliferation, apoptosis, migration assessed by Counting Kit-8 (CCK-8) assay, EdU staining, TUNEL transwell assay. binding miR-34a-5p VEGFA analyzed dual-luciferase accumulation HDAC9 lysine acetylation at H3 (H3K9, H3K14, H3K18) on promoter detected chromatin immunoprecipitation results showed that increased decreased tissues cells. Silencing inhibited apoptosis VECs, whereas overexpression exerted opposite functions. accumulated decrease reducing locus-specific further promoted expression. Knockdown or reversed protective silencing injury. In conclusion, our suggests may be a therapeutic target IA.

Язык: Английский

Процитировано

5
The functional mechanisms of synchronizing royal jelly consumption and physical activity on rat with multiple sclerosis-like behaviors hallmarks based on bioinformatics analysis, and experimental survey DOI Creative Commons

Maryam Lohrasbi,

Farzaneh Taghian, Khosro Jalali Dehkordi

и другие.

BMC Neuroscience, Год журнала: 2022, Номер 23(1)

Опубликована: Июнь 8, 2022

Natural nutrition and physical training have been defined as non-pharmacochemical complementary alternative medicines to prevent treat various pathogenesis. Royal jelly possesses pharmacological properties is an effective therapeutic supplement for halting neurodegeneration. Multiple sclerosis a prevalent neurodegenerative disorder that manifests progressive neurological condition. Inflammation, hypoxia, oxidative stress identified significant hallmarks of multiple pathology.In the present study, based on artificial intelligence bioinformatics algorithms, we marked hub genes, molecular signaling pathways, regulators such non-coding RNAs involved in sclerosis. Also, microRNAs can affect gene expression many processes. Numerous pathomechanisms, including immunodeficiency, stress, neuroinflammation, mitochondrial dysfunction, play role MSc pathogenesis results demyelination. Furthermore, computed binding affinity bioactive compounds presented Jelly macromolecules surfaces. predicted alignment score over pharmacophore model candidate protein novel approach. Based q-RT-PCR analysis, Dnajb1/Dnajb1/Foxp1/Tnfsf14 Hspa4 networks well miR-34a-5p miR155-3p were regulated by interaction exercise 100 mg/kg (ET-100RJ). Interestingly, characteristics, motor function, proinflammatory cytokine, demyelination ameliorated ET-100RJ.Here, indicated between had more effect regulating microRNA profiles genes rats with

Язык: Английский

Процитировано

8
The IRE1α Endonuclease Plays a Dual Role in Regulating the XBP1/miRNA-34a Axis and PD-1 Expression within Natural Killer Cells in Hodgkin Lymphoma DOI
Karolina Bednarska, Gayathri Thillaiyampalam,

Sally Mujaj

и другие.

Acta Haematologica, Год журнала: 2024, Номер unknown, С. 1 - 16

Опубликована: Март 14, 2024

<b><i>Introduction:</i></b> Hodgkin lymphoma (HL) is deficient in major histocompatibility complex class I, rendering it susceptible to antitumoral immunity by natural killer (NK) cells. Despite the functional impairment of PD-1<sup>+</sup> NK cells HL, underlying mechanisms cell dysfunction remain unclear. <b><i>Methods:</i></b> This study involved 14 HL patients and SNK10/KHYG-1 lines assess activation against cancer Activation was measured through transcript (PCR) protein expression (flow cytometry). Regulatory associated with IRE1α were validated knockdown luciferase reporter assays. <b><i>Results:</i></b> Our findings reveal a novel role for IRE1α-endonuclease fine-tuning effector functions orchestrating XBP1s/microRNA-34a-5p/PD-1 axis. When encounter cells, endonuclease activates decay microRNA-34a-5p, resulting increased XBP1s PD-1. enhances while promoting PD-1 expression. In turn, directly regulated which binds 3′UTR repress on surface. Importantly, IRE1α-pathway impaired from patients. <b><i>Conclusion:</i></b> The emerges as key player, simultaneously regulating axis process disrupted HL. Targeting holds promise therapeutic strategy optimize treatments.

Язык: Английский

Процитировано

1
miR‐34a promotes the immunosuppressive function of multiple myeloma‐associated macrophages by dampening the TLR‐9 signaling DOI Creative Commons
Rui Zhang,

Disi Zhang,

Yilan Luo

и другие.

Cancer Medicine, Год журнала: 2024, Номер 13(11)

Опубликована: Июнь 1, 2024

Promising outcomes have been observed in multiple myeloma (MM) with the use of immunotherapies, specifically chimeric antigen receptor T (CAR-T) cell therapy. However, a portion MM patients do not respond to CAR-T therapy, and reasons for this lack response remain unclear. The objective study was investigate impact miR-34a on immunosuppressive polarization macrophages obtained from patients.

Язык: Английский

Процитировано

1
Antinuclear antibodies in healthy population: Positive association with abnormal tissue metabolism, inflammation and immune dysfunction DOI
Qinghong Ge,

Xiuyu Gu,

Wen Yu

и другие.

International Immunopharmacology, Год журнала: 2022, Номер 113, С. 109292 - 109292

Опубликована: Окт. 14, 2022

Язык: Английский

Процитировано

7
Mechanism and function of miR-140 in human cancers: A review and in silico study DOI

Forough Taheri,

Seyed Omar Ebrahimi, Razieh Heidari

и другие.

Pathology - Research and Practice, Год журнала: 2022, Номер 241, С. 154265 - 154265

Опубликована: Дек. 7, 2022

Язык: Английский

Процитировано

7
Identification of diagnostic genes and vital microRNAs involved in rheumatoid arthritis: based on data mining and experimental verification DOI Creative Commons
Conglin Ren, Mingshuang Li, Yang Zheng

и другие.

PeerJ, Год журнала: 2021, Номер 9, С. e11427 - e11427

Опубликована: Май 14, 2021

The pathogenesis of rheumatoid arthritis (RA) is complex. This study aimed to identify diagnostic biomarkers and transcriptional regulators that underlie RA based on bioinformatics analysis experimental verification.We applied weighted gene co-expression network (WGCNA) analyze dataset GSE55457 obtained the key module most relevant phenotype. We then conducted function annotation, set enrichment (GSEA) immunocytes quantitative (CIBERSORT). Moreover, intersection differentially expressed genes (DEGs) within were entered into STRING database construct an interaction mine hub genes. predicted microRNA (miRNA) using a web-based tool (miRDB). Finally, vital miRNAs validated with independent GEO datasets, RT-qPCR Western blot.A total 367 DEGs characterized by differential expression analysis. WGCNA method divided 14 modules, we focused turquoise containing 845 Gene annotation GSEA suggested immune response inflammatory signaling pathways are molecular mechanisms behind RA. Nine screened from seven miRNA prediction. CIBERSORT identified five cell types enriched in samples, which closely related Through ROC curve validation, confirmed specific for RA, including CCL25, CXCL9, CXCL10, CXCL11, CXCL13. selected representative (CXCL10) blot validation. Vital verification showed only differences has-miR-573 has-miR-34a statistically significant.Our reveals microRNAs highly could help improve our understanding underlying disorder provide theoretical support future exploration innovative therapeutic approaches.

Язык: Английский

Процитировано

7