Molecular Medicine,
Год журнала:
2022,
Номер
28(1)
Опубликована: Июнь 17, 2022
At
present,
the
molecular
mechanisms
underlying
inflammation
remain
unclear.
In
recent
years,
research
on
has
focused
stimulating
cell
by
using
exogenous
pro-inflammatory
substances
such
as
lipopolysaccharide
(LPS)
or
inflammatory
factors.
To
investigate
mechanism
of
from
a
new
perspective,
we
designed
nucleic
acid
nanoflowers
(NFs)
complex
to
directly
activate
genes
study
response
without
need
for
external
microbial
factors
trigger
an
response.
An
RNAa-type
target
gene-activated
NFs
was
designed.
Human
umbilical
vein
endothelial
cells
(HUVECs)
were
transfected
with
carrying
small
activating
RNA
(saRNAs)
co-activate
microRNA
(miR)-155
and
SHIP1
genes.
After
activation
(RNAa)-type
transferred
into
HUVECs,
expression
miR-155
cancer-related
increased,
anti-inflammatory
reduced,
proliferation
migration
promoted.
IL-1β
protein
levels
decreased
downregulated.
When
its
both
activated,
unaltered,
maintaining
homeostasis.
This
points
towards
overexpression
can
inflammation,
that
act
switch
role
in
development
inflammation.
Frontiers in Endocrinology,
Год журнала:
2021,
Номер
12
Опубликована: Ноя. 4, 2021
Pancreatic
β-cells
within
the
islets
of
Langerhans
respond
to
rising
blood
glucose
levels
by
secreting
insulin
that
stimulates
uptake
peripheral
tissues
maintain
whole
body
energy
homeostasis.
To
different
extents,
failure
β-cell
function
and/or
loss
contribute
development
Type
1
and
2
diabetes.
Chronically
elevated
glycaemia
high
circulating
free
fatty
acids,
as
often
seen
in
obese
diabetics,
accelerate
disease.
MiRNAs
are
essential
for
endocrine
mature
pancreatic
dysregulated
In
this
review,
we
summarize
molecular
mechanisms
control
miRNA
expression
function,
including
transcription,
stability,
posttranscriptional
modifications,
interaction
with
RNA
binding
proteins
other
non-coding
RNAs.
We
also
discuss
which
these
responsible
nutrient-mediated
regulation
activity
miRNAs
identify
some
more
important
knowledge
gaps
field.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 19, 2023
Abstract
The
2019
pandemic,
caused
by
the
severe
acute
respiratory
syndrome
coronavirus
2
(SARS-CoV-2),
has
marked
spread
of
a
novel
human
coronavirus.
While
viral
life
cycle
is
well
understood,
most
interactions
at
virus-host
interface
remain
elusive.
Furthermore,
molecular
mechanisms
behind
disease
severity
and
immune
evasion
are
still
largely
unknown.
Conserved
elements
genome
such
as
secondary
structures
within
5’-
3’-untranslated
regions
(UTRs)
serve
attractive
targets
interest
could
prove
crucial
in
furthering
our
understanding
interactions.
It
been
proposed
that
microRNA
(miR)
with
components
be
used
both
virus
host
for
their
own
benefit.
Analysis
SARS-CoV-2
3’-UTR
revealed
potential
cellular
miR
binding
sites,
providing
sites
specific
virus.
In
this
study,
we
demonstrate
binds
miRNAs
miR-760-3p,
miR-34a-5p,
miR-34b-5p,
which
have
shown
to
influence
translation
interleukin-6
(IL-6),
IL-6
receptor
(IL-6R),
progranulin
(PGRN),
respectively,
proteins
roles
response
inflammatory
pathways.
recent
work
suggests
miR-34a-5p
miR-34b-5p
target
inhibit
proteins.
Native
gel
electrophoresis
steady-state
fluorescence
spectroscopy
were
utilized
characterize
these
miRs
predicted
3’-UTR.
Additionally,
investigated
2’-fluoro-D-arabinonucleic
acid
(FANA)
analogs
competitive
inhibitors
detailed
study
drive
development
antiviral
treatments
infection,
provide
basis
cytokine
release
implicate
host-virus
interface.
Author
Summary
Severe
(SARS-CoV-2)
now
plagued
world
over
three
years.
time,
scientific
advancements
allowed
mRNA
vaccines
targeted
drugs.
However,
many
cycle,
interface,
particular
combating
observed
dysregulation
mild
cases
infection.
To
uncover
link
between
infection
dysregulation,
microRNAs
associated
response,
particularly
emphasize
them
We
biophysical
methods
Lastly,
introduce
disruptors
interactions,
intent
therapeutic
intervention.
Obstetrics & Gynecology Science,
Год журнала:
2023,
Номер
67(1), С. 30 - 48
Опубликована: Дек. 5, 2023
Polycystic
ovarian
syndrome
(PCOS)
is
a
common
endocrine
disorder
in
women
of
reproductive
age.
The
clinical
symptoms
include
hyperandrogenism,
chronic
anovulation,
and
multiple
cysts.
PCOS
strongly
associated
with
obesity
insulin
resistance.
MicroRNAs
(miRNAs)
are
group
short
non-coding
RNAs
that
play
role
the
post-transcriptional
regulation
gene
expression
translational
inhibition.
They
vital
metabolic
hormonal
processes
as
well
oocyte
maturation
folliculogenesis
female
system.
miRNAs
can
be
used
diagnostic
biomarkers
or
therapeutic
targets
because
their
stability.
encapsulation
extracellular
vesicles
exosomes
contributes
to
Exosomes
constantly
secreted
by
many
cells
size
about
30
150
nm.
Enveloping
release
them
for
cellular
communication.
induced
transfer
novel
process
genetic
exchange
between
cells.
Many
studies
have
shown
along
non-exosomal
miRNAs,
different
types
exosomal
derived
from
serum
follicular
fluid
an
essential
pathogenesis.
These
involved
development
various
functions
granulosa
cells,
apoptosis,
cell
proliferation,
atresia.
present
study
aimed
comprehensively
review
evidence
on
affected
pathways
under
both
circumstances,
primarily
focusing
pathogenesis
PCOS.
Molecular Medicine,
Год журнала:
2022,
Номер
28(1)
Опубликована: Июнь 17, 2022
At
present,
the
molecular
mechanisms
underlying
inflammation
remain
unclear.
In
recent
years,
research
on
has
focused
stimulating
cell
by
using
exogenous
pro-inflammatory
substances
such
as
lipopolysaccharide
(LPS)
or
inflammatory
factors.
To
investigate
mechanism
of
from
a
new
perspective,
we
designed
nucleic
acid
nanoflowers
(NFs)
complex
to
directly
activate
genes
study
response
without
need
for
external
microbial
factors
trigger
an
response.
An
RNAa-type
target
gene-activated
NFs
was
designed.
Human
umbilical
vein
endothelial
cells
(HUVECs)
were
transfected
with
carrying
small
activating
RNA
(saRNAs)
co-activate
microRNA
(miR)-155
and
SHIP1
genes.
After
activation
(RNAa)-type
transferred
into
HUVECs,
expression
miR-155
cancer-related
increased,
anti-inflammatory
reduced,
proliferation
migration
promoted.
IL-1β
protein
levels
decreased
downregulated.
When
its
both
activated,
unaltered,
maintaining
homeostasis.
This
points
towards
overexpression
can
inflammation,
that
act
switch
role
in
development
inflammation.
