Frontiers in Microbiology,
Год журнала:
2023,
Номер
14
Опубликована: Июль 27, 2023
Diabetic
nephropathy
(DN)
is
a
severe
microvascular
complication
of
diabetes,
which
increases
the
risk
renal
failure
and
causes
high
global
disease
burden.
Due
to
lack
sustainable
treatment,
DN
has
become
primary
cause
end-stage
worldwide.
Gut
microbiota
its
metabolites
exert
critical
regulatory
functions
in
maintaining
host
health
are
associated
with
many
pathogenesis
aging-related
chronic
diseases.
Currently,
theory
gut–kidney
axis
opened
novel
angle
understand
relationship
between
gut
multiple
kidney
In
recent
years,
accumulating
evidence
revealed
that
their
play
an
essential
role
pathophysiologic
processes
through
axis.
this
review,
we
summarize
current
investigations
microbial
involvement
progression
DN,
further
discuss
potential
microbiota-targeted
therapeutic
approaches
for
DN.
Biomedicine & Pharmacotherapy,
Год журнала:
2024,
Номер
177, С. 117008 - 117008
Опубликована: Июнь 19, 2024
Astragaloside
IV
(AS-IV)
exhibits
diverse
biological
activities.
Despite
this,
the
detailed
molecular
mechanisms
by
which
AS-IV
ameliorates
diabetic
nephropathy
(DN)
and
shields
podocytes
from
oxidative
stress
(OS)
mitochondrial
dysfunction
remain
poorly
understood.
In
this
study,
we
used
biochemical
assays,
histopathological
analysis,
Doppler
ultrasound,
transmission
electron
microscopy，flow
cytometry,
fluorescence
staining,
Western
blotting
other
methods.
was
administered
to
db/db
mice
for
in
vivo
experimentation.
Our
findings
indicated
that
treatment
significantly
reduced
diabetes-associated
markers,
proteinuria,
kidney
damage.
It
also
diminished
ROS
levels
kidney,
enhanced
expression
of
endogenous
antioxidant
enzymes,
improved
health.
Phenyl
sulfate
(PS),
a
protein-bound
uremic
solute
enteric
origin,
has
been
closely
linked
with
DN
represents
promising
avenue
further
research.
vitro,
PS
exposure
induced
OS
podocytes,
increasing
while
decreasing
enzyme
activity
(Catalase,
Heme
Oxygenase-1,
Superoxide
Dismutase,
Glutathione
Peroxidase).
inhibitors
(N-acetyl-L-cysteine,
NAC)
as
positive
control
group
can
reduce
restore
enzymes
protein
levels.
Additionally,
markers
associated
biosynthesis
function
(SIRT1,
PGC1α,
Nrf1,
TFAM).
These
adverse
effects
were
partially
reversed
treatment.
However,
co-treatment
SIRT1
inhibitor
EX527
failed
these
indicators.
Overall,
our
study
demonstrates
effectively
attenuates
mitigates
PS-induced
via
SIRT1/PGC1α/Nrf1
pathway.
Diabetology & Metabolic Syndrome,
Год журнала:
2025,
Номер
17(1)
Опубликована: Март 28, 2025
This
study
aimed
to
evaluate
the
associations
between
neutrophil
percentage-to-albumin
ratio
(NPAR)
and
both
all-cause
cardiovascular
mortality
in
diabetic
kidney
disease
(DKD)
patients.
The
data
for
this
were
sourced
from
National
Health
Nutrition
Examination
Survey
(NHANES)
1999–2018.
Weighted
logistic
regression,
Cox
proportional
hazards
model,
Fine-Gray
competing
risk
model
used
assess
association
NPAR
DKD
A
total
of
2,699
participants
enrolled
study.
regression
analysis
revealed
that
elevated
levels
associated
with
a
higher
all
(HR:
2.17,
95%CI:
1.83–2.58).
Meanwhile,
significant
difference
was
observed
males
1.83,
1.42–2.38)
but
not
females.
Finally,
adjusted
identified
as
an
independent
predictor
(SHR:
1.86
1.28–2.72)
In
nationally
representative
sample
US,
detected
increased
mortality.
addition,
gender
differences
relationship
also
observed.
Diabetes Metabolic Syndrome and Obesity,
Год журнала:
2024,
Номер
Volume 17, С. 75 - 92
Опубликована: Янв. 1, 2024
Background:
Diabetic
kidney
disease
(DKD)
is
characterized
by
renal
fibrosis,
and
the
pathogenesis
of
fibrosis
still
not
definitely
confirmed.
MiR-204-5p
plays
an
important
role
in
regulation
autophagy
oxidative
stress.
In
this
study,
we
aimed
to
investigate
miR-204-5p
on
damage
diabetic
kidneys
underlying
mechanisms
involved.
Methods:
vivo,
AAV-Ksp-miR-204-5p
mimics
were
injected
into
mice
via
tail
vein.
vitro,
high
glucose-induced
HK-2
cells
treated
with
inhibitor,
mimics,
ATG5
siRNA,
tertiary
butyl
hydroquinone
(TBHQ),
ML385,
or
3-Methyladenine
(3-MA).
FISH
qRT-PCR
used
detect
expression.
The
expressions
protein
mRNA
detected
Western
blotting,
immunofluorescence,
immunohistochemistry
qRT-PCR.
concentration
fibronectin
culture
medium
was
ELISA.
Results:
expression
significantly
inhibited
than
that
control
group.
Delivering
increased
expression,
improved
function,
stress,
restored
db/db
mice.
HG
treatment
cells.
effectively
reduced
collagen
I
dysfunction,
Nrf2
whereas
these
alterations
abrogated
inhibitor
3-methyladenine
(3-MA,
5
mM)
siRNA
transfection
HG-induced
addition,
aggravated
decreased
while
abolished
activator
TBHQ.
Furthermore,
binding
Keap1
confirmed
luciferase
reporter
assay
negatively
regulated
resulting
activation
pathway.
Conclusion:
MicroRNA-204-5p
protects
against
progression
restoring
regulating
Keap1/Nrf2
Keywords:
disease,
miR-204-5p,
Keap1,
Nrf2,
Frontiers in Endocrinology,
Год журнала:
2024,
Номер
14
Опубликована: Янв. 4, 2024
Systemic
immune-inflammation
index
(SII),
a
novel
inflammatory
marker,
has
been
reported
to
be
associated
with
diabetic
kidney
disease
(DKD)
in
the
U.S.,
however,
such
close
relationship
DKD
other
countries,
including
China,
not
never
determined.
We
aimed
explore
association
between
SII
and
Chinese
population.
A
total
of
1922
hospitalized
patients
type
2
diabetes
mellitus
(T2DM)
included
this
cross-sectional
study
were
divided
into
three
groups
based
on
estimated
glomerular
filtration
rate
(eGFR)
urinary
albumin-to-creatinine
ratio
(ACR):
non-DKD
group,
stages
1-2
Alb
DKD-non-Alb+DKD
stage
3
group.
The
possible
was
investigated
by
correlation
multivariate
logistic
regression
analysis,
receiver-operating
characteristic
(ROC)
curves
analysis.
Moving
from
group
level
gradually
increased
(P
for
trend
<0.01).
Partial
analysis
revealed
that
positively
ACR
prevalence
DKD,
negatively
eGFR
(all
P<0.01).
Multivariate
showed
remained
independently
significantly
presence
after
adjustment
all
confounding
factors
[(odds
(OR),
2.735;
95%
confidence
interval
(CI),
1.840-4.063;
P
<
0.01)].
Moreover,
compared
subjects
lowest
quartile
(Q1),
fully
adjusted
OR
1.060
(95%
CI
0.773-1.455)
Q2,
1.167
0.995-1.368)
Q3,
1.266
1.129-1.420)
highest
(Q4)
Similar
results
observed
or
DKD-non-
Alb+DKD
among
quartiles.
Last,
ROC
best
cutoff
values
predict
1-
2,
DKD-non-Alb+
609.85
(sensitivity:
48.3%;
specificity:
72.8%),
601.71
43.9%;
72.3%),
589.27
61.1%;
71.1%),
respectively.
Higher
is
an
risk
severity
might
promising
biomarker
its
distinct
phenotypes
