Background:
Metformin
is
a
first-line
drug
widely
used
in
the
treatment
of
type
2
diabetes.
It
has
been
shown
to
improve
liver
fibrosis;
however,
underlying
mechanism
remains
unclear.
Objectives:
This
study
investigates
relationship
between
metformin
and
gut
microbiota
fibrosis.
Methods:
Carbon
tetrachloride
(CCl₄)
was
injected
intraperitoneally
induce
fibrosis
mice,
followed
by
for
4
weeks.
A
CCl₄-induced
hepatic
mouse
model
established.
The
mice
were
divided
into
two
groups:
control
groups.
Hematoxylin-eosin
(HE)
staining
evaluate
overall
condition,
Sirius
red
assess
degree
fibrosis,
α-smooth
muscle
actin
(α-SMA)
immunohistochemical
determine
activation
stellate
cells
(HSCs),
oil
O
observe
intracellular
lipid
droplets
hepatocytes,
16sRNA
sequencing
analyze
fecal
microbiota.
Results:
In
untreated
model,
tissue
exhibited
edema,
degeneration,
deposition
inflammatory
cell
infiltration
lobules.
attenuated
(area
reduced
from
1.383%
0.669%,
P
<
0.001),
decreased
infiltration,
accumulation
hepatocytes
0.457%
0.086%,
α-SMA
protein
expression
1.509%
0.598%,
0.05).
Additionally,
harmful
bacteria
increased
abundance
beneficial
intestinal
tract
mice.
Conclusions:
These
findings
indicate
that
reduces
inflammation,
alleviates
alters
modification
flora
may
be
significant
which
exerts
its
therapeutic
effects
on
Biomolecules,
Год журнала:
2024,
Номер
14(7), С. 873 - 873
Опубликована: Июль 20, 2024
Guanylate
binding
protein
5
(GBP5)
is
an
emerging
immune
component
that
has
been
increasingly
recognized
for
its
involvement
in
autoimmune
diseases,
particularly
inflammatory
bowel
disease
(IBD).
IBD
a
complex
involving
inflammation
of
the
gastrointestinal
tract.
Here,
we
explored
functional
significance
GBP5
using
Microbiological Research,
Год журнала:
2025,
Номер
293, С. 128053 - 128053
Опубликована: Янв. 6, 2025
Insufficiency
of
Akkermansia
muciniphila
(Akk)
has
been
implicated
in
the
pathogenesis
metabolic
diseases,
and
administration
or
restoration
Akk
ameliorated
these
disorders.
Recently,
Pasteurized
(PA-Akk)
approved
as
a
functional
food.
However,
impact
on
lipid
absorption
proximal
intestine,
which
is
directly
exposed
to
orally
administered
Akk,
remains
largely
unexplored.
In
this
study,
we
PA-Akk
mice
investigated
subsequent
absorption.
Long-term
resulted
reduced
deposits
liver
adipocytes,
along
with
improved
glucose
metabolism.
This
was
primarily
attributed
reduction
by
epithelial
cells
jejunum.
Mechanistically,
activated
AMP-activated
protein
kinase
(AMPK)
inhibit
lipids
both
mouse
human
jejunal
cells.
Furthermore,
demonstrated
that
treatment,
but
not
promotes
abundance
genera
are
highly
abundant
normal
jejunum
belong
phylum
Firmicutes.
Thus,
our
study
concludes
oral
provides
beneficial
effects
metabolism,
partially
through
inhibiting
promoting
core
microbes.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 1360 - 1360
Опубликована: Фев. 6, 2025
Non-alcoholic
fatty
liver
disease
(NAFLD),
the
most
prevalent
chronic
illness,
is
characterized
by
hepatic
steatosis.
Exercise
and
probiotics
can
regulate
gut
microbiota
to
treat
NAFLD;
however,
their
combined
effects
mechanisms
of
gut-liver
communication
remain
unclear.
Inconsistent
results
on
probiotic
efficacy
further
warrant
investigation.
In
this
study,
zebrafish
fed
a
high-fat
diet
(HFD)
for
six
weeks
were
subjected
swimming
exercise
(HFDE),
intervention
(HFDP),
or
combination
both
(HFDEP)
10
explore
NAFLD
corresponding
mechanism.
The
showed
that
alleviation
followed
order
HFDEP
>
HFDE
HFDP.
treatments
effectively
reduced
Body
Mass
Index
(BMI),
relative
weight,
vacuolation
density,
lipid
droplets
in
sections,
triglyceride,
free
acid,
glucose,
pyruvic
acid.
contrast,
single
treatment
had
limited
impact,
suggesting
complementary
role
treatment.
Glucose
acid
metabolism
central
“gut–liver”
axis.
conversion
glucose
decreased
synthesis
esterification,
accelerated
transformation
CO2
contributed
improvement
under
treatments.
This
study
provides
promising
theoretical
groundwork
potential
prevention
strategies
NAFLD.
Frontiers in Cellular and Infection Microbiology,
Год журнала:
2025,
Номер
15
Опубликована: Март 14, 2025
The
intestinal
microbiota
is
important
for
the
health
of
host
and
recent
studies
have
shown
that
some
genes
regulated
composition
microbiota.
Group
10
phospholipase
A2
(PLA2G10)
a
member
lipolytic
enzyme
family
PLA2,
which
hydrolyze
ester
bond
at
sn-2
position
phospholipids
to
produce
free
fatty
acids
lysophospholipids.
PLA2G10
secreted
into
lumen,
but
its
impact
on
gut
remains
unclear.
In
this
study,
we
generated
intestine-specific
Pla2g10
knock-in
mice,
used
16S
RNA
sequencing
compare
their
with
wild-type
(WT)
littermates.
Results
showed
gut-specific
induced
both
mRNA
protein
levels
in
colon.
Moreover,
overexpression
reduced
α-diversity
relative
WT
mice.
abundance
Bacteroidetes
was
lower
mice
than
control
while
ratio
Firmicutes/Bacteroidetes
higher.
Furthermore,
genus
Allobaculum
reduced,
whereas
beneficial
bacteria
genera,
including
Enterorhabdus,
Dubosiella,
Lactobacillus,
increased
by
overexpression.
summary,
proportions
bacterial
colonic
chyme
providing
potential
therapeutic
target
future
improvement
Frontiers in Nutrition,
Год журнала:
2025,
Номер
12
Опубликована: Март 26, 2025
Introduction
Obesity
is
a
complex
metabolic
disease,
which
often
accompanied
with
impaired
glucose
and
lipid
metabolism
chronic
inflammation.
Probiotics
have
been
considered
as
strategy
for
treating
obesity,
while
the
genus
of
Lactobacillus
most
commonly
tested
approved
probiotics.
Some
multi-strain
probiotics
were
proven
to
produce
synergistic
effects
on
obesity
compared
mono-strain
ones.
Methods
The
purpose
this
study
was
investigate
anti-obesity
effect
new
probiotic
formation
contained
plantarum
L14,
paracasei
L9,
rhamnosus
GG,
sakei
X-MRS-2,
designated
L-PPRS.
Multi-strain
L-PPRS
shown
better
antiadipogenic
than
in
3T3-L1
cell.
Subsequently,
orally
supplemented
high-fat
diet
(HFD)
induced
obese
mouse
model
two
kinds
treatment
course,
short-term
(8
weeks)
one
long-term
(12
one.
Results
We
found
that
intervention
not
only
significantly
inhibited
weight
gain
HFD-fed
mice,
but
also
improved
tolerance,
insulin
sensitivity
reduced
serum
levels.
Furthermore,
fat
accumulation
adipose
tissue
liver,
ameliorated
antioxidant
capacity
liver
mice.
modulated
expression
lipid-metabolic
genes,
exhibited
excellent
anti-inflammatory
effect.
In
addition,
restored
dysbiosis
gut
microbiota
via
reducing
Firmicutes/
Bacteroidetes
ratio,
increasing
abundance
beneficial
intestinal
bacteria.
conclusion,
proved
could
effectively
prevent
development
its
associated
abnormalities,
supplementation
provided
more
profound
benefit
short-term.
Discussion
This
highlights
potential
an
effective
strategy.
Different
LNT
doses
promoted
the
proliferation
of
probiotics
with
different
functions,
which
prevented
liver
function
impairment,
oxidative
stress,
and
inflammation
caused
by
acute
excessive
alcohol
consumption.
