ΔNP63 defines an exocrine-committed multipotent progenitor subset in the murine pancreas DOI Creative Commons
Katarina Coolens,

Marijke van der Vliet,

Jente Van Campenhout

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

Abstract Cellular plasticity underpins heterogeneity in embryogenic progenitor cells and cancer cells. The transcription factor deltaNp63 ( ΔNp63 ) has been implicated regulating cellular several epithelial tissues. Despite a recently established role steering of pancreatic cancer, remains unstudied development. Using murine single-cell sequencing data RNA protein situ stainings, we assessed the spatio-temporal expression Trp63 ΔNP63 embryonic pancreas. demonstrates transient spatially restricted multipotent (MPP) compartment delineating pro-exocrine Lineage tracing TP63 + marks subset MPPs descendant exocrine acinar centro-acinar/terminal duct Lack knock-out mice leads to hypotrophic acini with reduced levels differentiation markers. In summary, confers within MPP compartment, supporting cell These new insights developmental have potential implications for regeneration cancer.

Язык: Английский

Senescence screening of mesenchymal stromal cells by morphology-based deep learning senescence analysis DOI Creative Commons

Liudi Wang,

Haijie Zhang,

Jianwei Chen

и другие.

Biomedical Signal Processing and Control, Год журнала: 2025, Номер 104, С. 107549 - 107549

Опубликована: Янв. 26, 2025

Язык: Английский

Процитировано

0
Cistanche deserticola polysaccharides enhance female germline stem cell differentiation through BMP/SMAD signaling to mitigate premature ovarian failure DOI
Yikai Qiu, Jinhua Li, Yanping Zhang

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140848 - 140848

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

0
Many Faces of Regulatory T Cells: Heterogeneity or Plasticity? DOI Creative Commons
V. G. Blinova, Dmitry D. Zhdanov

Cells, Год журнала: 2024, Номер 13(11), С. 959 - 959

Опубликована: Июнь 1, 2024

Regulatory T cells (Tregs) are essential for maintaining the immune balance in normal and pathological conditions. In autoimmune diseases transplantation, they restrain loss of self-tolerance promote engraftment, whereas cancer, an increase Treg numbers is mostly associated with tumor growth poor prognosis. Numerous markers their combinations have been used to identify subsets, demonstrating phenotypic diversity Tregs. The complexity identification can be hampered by unstable expression some markers, decrease a specific marker over time or emergence new marker. It remains unclear whether such shifts due conditions observed changes initially different populations. first case, cellular plasticity observed, second, heterogeneity observed. difference between these terms relation Tregs rather blurred. Considering promising perspectives regenerative cell-based therapy, existing confusing data on phenotypes require further investigation analysis. our review, we introduce criteria that allow us distinguish normally pathologically, taking closer look at drawing line two terms.

Язык: Английский

Процитировано

3
Current status and perspectives for 3D biomimetic epidermal tissue: From tissue reconstruction to biomedical application DOI

Shengsheng He,

Zhiyuan Wang, Rui Wang

и другие.

Journal of Drug Delivery Science and Technology, Год журнала: 2024, Номер 97, С. 105722 - 105722

Опубликована: Апрель 26, 2024

Язык: Английский

Процитировано

2
Single-cell RNA sequencing reveals vascularization-associated cell subpopulations in dental pulp: PDGFRβ+ DPSCs with activated PI3K/AKT pathway DOI Creative Commons

Tiankai Di,

Liying Wang,

Baixiang Cheng

и другие.

Stem Cells, Год журнала: 2024, Номер 42(10), С. 914 - 927

Опубликована: Авг. 21, 2024

Abstract Background This study aims to address challenges in dental pulp regeneration therapy. The heterogeneity of DPSCs poses challenges, especially stem cell transplantation for clinical use, particularly when sourced from donors different ages and conditions. Methods Pseudotime analysis was employed analyze single-cell sequencing data, immunohistochemical studies were conducted investigate the expression fibronectin 1 (FN1). We performed vitro sorting PDGFRβ+ using flow cytometry. A series functional assays, including proliferation, scratch, tube formation experimentally validate vasculogenic capabilities identified DPSC subset. Furthermore, gene-edited mouse models utilized demonstrate importance DPSCs. Transcriptomic compare differences between P1-DPSCs. Results Single-cell unveiled a distinct subset, DPSCs, characterized by significantly elevated FN1 during development. Subsequent experiments demonstrated that this subset possesses remarkable abilities promote HUVEC migration, formation. Gene-edited confirmed vital role PDGFR/PI3K/AKT signaling pathway is crucial factor mediating proliferation rate pro-angiogenic properties Conclusion defined new strong proliferative activity capabilities, demonstrating significant translational potential.

Язык: Английский

Процитировано

2
Expression of calpastatin hcast 3-25 and activity of the calpain/calpastatin system in human glioblastoma stem cells: possible involvement of hcast 3-25 in cell differentiation DOI Creative Commons
Sonia Spinelli, Federica Barbieri, Monica Averna

и другие.

Frontiers in Molecular Biosciences, Год журнала: 2024, Номер 11

Опубликована: Июль 30, 2024

Glioblastoma (GBM) is the most malignant brain tumor, characterized by cell heterogeneity comprising stem cells (GSCs) responsible for aggressiveness. The calpain/calpastatin (calp/cast) proteolytic system involved in critical physiological processes and cancer progression. In this work we showed expression profile of hcast 3-25 (a Type III calpastatin variant devoid inhibitory units) members several patient-derived GSCs exploring relationship between activation/activity calpains. Each GSC shows a peculiar calp/cast mRNA protein pattern, least expressed. Differentiation promotes upregulation all components except mRNA, which increased or decreased depending on individual culture. Transfection 3-25-V5 into two selected indicated that effectively associates with calpains, supporting digestion calpain targets. Hcast possibly affects state promoting differentiated, less aggressive phenotype.

Язык: Английский

Процитировано

1
Sarcopenia and the biological determinants of aging: A narrative review from a geroscience perspective DOI Creative Commons

Mariá Nunes-Pinto,

Renato Gorga Bandeira de Mello,

M. Pinto

и другие.

Ageing Research Reviews, Год журнала: 2024, Номер 103, С. 102587 - 102587

Опубликована: Ноя. 19, 2024

Язык: Английский

Процитировано

1
An in-silico approach to the dynamics of proliferation potential in stem cells and the study of different therapies in cases of ovarian dysfunction DOI
A.M. Portillo, Juan A. García-Velasco, Elisa Varela

и другие.

Mathematical Biosciences, Год журнала: 2024, Номер 377, С. 109305 - 109305

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

0
ΔNP63 defines an exocrine-committed multipotent progenitor subset in the murine pancreas DOI Creative Commons
Katarina Coolens,

Marijke van der Vliet,

Jente Van Campenhout

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 16, 2024

Abstract Cellular plasticity underpins heterogeneity in embryogenic progenitor cells and cancer cells. The transcription factor deltaNp63 ( ΔNp63 ) has been implicated regulating cellular several epithelial tissues. Despite a recently established role steering of pancreatic cancer, remains unstudied development. Using murine single-cell sequencing data RNA protein situ stainings, we assessed the spatio-temporal expression Trp63 ΔNP63 embryonic pancreas. demonstrates transient spatially restricted multipotent (MPP) compartment delineating pro-exocrine Lineage tracing TP63 + marks subset MPPs descendant exocrine acinar centro-acinar/terminal duct Lack knock-out mice leads to hypotrophic acini with reduced levels differentiation markers. In summary, confers within MPP compartment, supporting cell These new insights developmental have potential implications for regeneration cancer.

Язык: Английский

Процитировано

0