ΔNP63 defines an exocrine-committed multipotent progenitor subset in the murine pancreas DOI Creative Commons
Katarina Coolens,

Marijke van der Vliet,

Jente Van Campenhout

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Cellular plasticity underpins heterogeneity in embryogenic progenitor cells and cancer cells. The transcription factor deltaNp63 ( ΔNp63 ) has been implicated regulating cellular several epithelial tissues. Despite a recently established role steering of pancreatic cancer, remains unstudied development. Using murine single-cell sequencing data RNA protein situ stainings, we assessed the spatio-temporal expression Trp63 ΔNP63 embryonic pancreas. demonstrates transient spatially restricted multipotent (MPP) compartment delineating pro-exocrine Lineage tracing TP63 + marks subset MPPs descendant exocrine acinar centro-acinar/terminal duct Lack knock-out mice leads to hypotrophic acini with reduced levels differentiation markers. In summary, confers within MPP compartment, supporting cell These new insights developmental have potential implications for regeneration cancer.

Language: Английский

Senescence screening of mesenchymal stromal cells by morphology-based deep learning senescence analysis DOI Creative Commons

Liudi Wang,

Haijie Zhang,

Jianwei Chen

et al.

Biomedical Signal Processing and Control, Journal Year: 2025, Volume and Issue: 104, P. 107549 - 107549

Published: Jan. 26, 2025

Language: Английский

Citations

0
Cistanche deserticola polysaccharides enhance female germline stem cell differentiation through BMP/SMAD signaling to mitigate premature ovarian failure DOI
Yikai Qiu, Jinhua Li, Yanping Zhang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 140848 - 140848

Published: Feb. 1, 2025

Language: Английский

Citations

0
Many Faces of Regulatory T Cells: Heterogeneity or Plasticity? DOI Creative Commons
V. G. Blinova, Dmitry D. Zhdanov

Cells, Journal Year: 2024, Volume and Issue: 13(11), P. 959 - 959

Published: June 1, 2024

Regulatory T cells (Tregs) are essential for maintaining the immune balance in normal and pathological conditions. In autoimmune diseases transplantation, they restrain loss of self-tolerance promote engraftment, whereas cancer, an increase Treg numbers is mostly associated with tumor growth poor prognosis. Numerous markers their combinations have been used to identify subsets, demonstrating phenotypic diversity Tregs. The complexity identification can be hampered by unstable expression some markers, decrease a specific marker over time or emergence new marker. It remains unclear whether such shifts due conditions observed changes initially different populations. first case, cellular plasticity observed, second, heterogeneity observed. difference between these terms relation Tregs rather blurred. Considering promising perspectives regenerative cell-based therapy, existing confusing data on phenotypes require further investigation analysis. our review, we introduce criteria that allow us distinguish normally pathologically, taking closer look at drawing line two terms.

Language: Английский

Citations

3
Current status and perspectives for 3D biomimetic epidermal tissue: From tissue reconstruction to biomedical application DOI

Shengsheng He,

Zhiyuan Wang, Rui Wang

et al.

Journal of Drug Delivery Science and Technology, Journal Year: 2024, Volume and Issue: 97, P. 105722 - 105722

Published: April 26, 2024

Language: Английский

Citations

2
Single-cell RNA sequencing reveals vascularization-associated cell subpopulations in dental pulp: PDGFRβ+ DPSCs with activated PI3K/AKT pathway DOI Creative Commons

Tiankai Di,

Liying Wang,

Baixiang Cheng

et al.

Stem Cells, Journal Year: 2024, Volume and Issue: 42(10), P. 914 - 927

Published: Aug. 21, 2024

Abstract Background This study aims to address challenges in dental pulp regeneration therapy. The heterogeneity of DPSCs poses challenges, especially stem cell transplantation for clinical use, particularly when sourced from donors different ages and conditions. Methods Pseudotime analysis was employed analyze single-cell sequencing data, immunohistochemical studies were conducted investigate the expression fibronectin 1 (FN1). We performed vitro sorting PDGFRβ+ using flow cytometry. A series functional assays, including proliferation, scratch, tube formation experimentally validate vasculogenic capabilities identified DPSC subset. Furthermore, gene-edited mouse models utilized demonstrate importance DPSCs. Transcriptomic compare differences between P1-DPSCs. Results Single-cell unveiled a distinct subset, DPSCs, characterized by significantly elevated FN1 during development. Subsequent experiments demonstrated that this subset possesses remarkable abilities promote HUVEC migration, formation. Gene-edited confirmed vital role PDGFR/PI3K/AKT signaling pathway is crucial factor mediating proliferation rate pro-angiogenic properties Conclusion defined new strong proliferative activity capabilities, demonstrating significant translational potential.

Language: Английский

Citations

2
Expression of calpastatin hcast 3-25 and activity of the calpain/calpastatin system in human glioblastoma stem cells: possible involvement of hcast 3-25 in cell differentiation DOI Creative Commons
Sonia Spinelli, Federica Barbieri, Monica Averna

et al.

Frontiers in Molecular Biosciences, Journal Year: 2024, Volume and Issue: 11

Published: July 30, 2024

Glioblastoma (GBM) is the most malignant brain tumor, characterized by cell heterogeneity comprising stem cells (GSCs) responsible for aggressiveness. The calpain/calpastatin (calp/cast) proteolytic system involved in critical physiological processes and cancer progression. In this work we showed expression profile of hcast 3-25 (a Type III calpastatin variant devoid inhibitory units) members several patient-derived GSCs exploring relationship between activation/activity calpains. Each GSC shows a peculiar calp/cast mRNA protein pattern, least expressed. Differentiation promotes upregulation all components except mRNA, which increased or decreased depending on individual culture. Transfection 3-25-V5 into two selected indicated that effectively associates with calpains, supporting digestion calpain targets. Hcast possibly affects state promoting differentiated, less aggressive phenotype.

Language: Английский

Citations

1
Sarcopenia and the biological determinants of aging: A narrative review from a geroscience perspective DOI Creative Commons

Mariá Nunes-Pinto,

Renato Gorga Bandeira de Mello,

M. Pinto

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 103, P. 102587 - 102587

Published: Nov. 19, 2024

Language: Английский

Citations

1
An in-silico approach to the dynamics of proliferation potential in stem cells and the study of different therapies in cases of ovarian dysfunction DOI
A.M. Portillo, Juan A. García-Velasco, Elisa Varela

et al.

Mathematical Biosciences, Journal Year: 2024, Volume and Issue: 377, P. 109305 - 109305

Published: Oct. 2, 2024

Language: Английский

Citations

0
ΔNP63 defines an exocrine-committed multipotent progenitor subset in the murine pancreas DOI Creative Commons
Katarina Coolens,

Marijke van der Vliet,

Jente Van Campenhout

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 16, 2024

Abstract Cellular plasticity underpins heterogeneity in embryogenic progenitor cells and cancer cells. The transcription factor deltaNp63 ( ΔNp63 ) has been implicated regulating cellular several epithelial tissues. Despite a recently established role steering of pancreatic cancer, remains unstudied development. Using murine single-cell sequencing data RNA protein situ stainings, we assessed the spatio-temporal expression Trp63 ΔNP63 embryonic pancreas. demonstrates transient spatially restricted multipotent (MPP) compartment delineating pro-exocrine Lineage tracing TP63 + marks subset MPPs descendant exocrine acinar centro-acinar/terminal duct Lack knock-out mice leads to hypotrophic acini with reduced levels differentiation markers. In summary, confers within MPP compartment, supporting cell These new insights developmental have potential implications for regeneration cancer.

Language: Английский

Citations

0