Future Directions and Pipeline Therapies for Hidradenitis Suppurativa
Dermatologic Clinics,
Год журнала:
2025,
Номер
43(2), С. 359 - 371
Опубликована: Янв. 21, 2025
Язык: Английский
Single-cell transcriptomics of clinical grade adipose-derived regenerative cells reveals consistency between donors independent of gender and BMI
Stem Cell Research & Therapy,
Год журнала:
2025,
Номер
16(1)
Опубликована: Март 5, 2025
Adipose-derived
regenerative
cells
(ADRCs)
also
referred
to
as
the
stromal
vascular
fraction,
provide
an
ample
source
of
stem
with
widespread
therapeutic
use.
Being
heterogenous
in
nature,
possibly
affecting
clinical
outcome
after
cell
treatment,
ADRC-
donor,
-BMI,
and
-gender
may
have
a
large
impact
on
ADRC
composition
quality
but
this
remains
largely
unexplored.
Herein,
we
comprehensive
single-cell
RNA
sequencing
mapping
across
two
trial
intervention
studies
found
no
gender-
or
BMI-related
variations,
except
for
minor
female
increase
PI16/CD55-expressing
cells.
Indeed,
heterogeneity
was
surprisingly
minimal
between
donors.
This
provides
important
decision-making
support
adipose
donor
selection
treatments,
suggest
that
gender
BMI
should
be
regarded
less
influential.
Язык: Английский
Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow
Journal of Biological Engineering,
Год журнала:
2025,
Номер
19(1)
Опубликована: Март 14, 2025
Abstract
Adipose-derived
stem
cells
(ASCs)
provide
an
ample,
easily
accessible
source
of
multipotent
cells,
alternative
to
bone
marrow-derived
stromal
(BMSCs),
capable
differentiating
into
osteoblasts.
However,
the
osteogenic
potential
ASCs
is
reportedly
lower
than
that
BMSCs
and
protocols
effectively
differentiate
osteoblasts
are
in
high
demand.
Here,
we
present
novel
strategies
for
effective
differentiation
human
by
combining
their
culture
on
bioactive
growth
surfaces
with
treatment
specific
supplements
medium
application
fluid
shear
stress.
Human
were
cultured
PLGA-based
composites
containing
50
wt%
sol-gel
glasses
(SBGs)
from
SiO
2
-CaO±P
O
5
system,
either
unmodified
or
modified
ZnO
SrO.
The
was
supplemented
recombinant
morphogenetic
protein
(BMP-2),
MEK1/2
kinase
inhibitor
(PD98059)
indirect
Smurf1
(Phenamil).
Fluid
stress
applied
a
standard
horizontal
rocker.
ASC
SBG-PLGA
along
enhanced
expression
both
early
late
markers.
Modification
SBG
SrO
further
gene
compared
SBGs.
Notably,
synergistically
strengthened
effects
supplements.
We
also
show
presented
can
drive
toward
osteoblastic
3-day
period
mineralizing
through
short,
7-day
preculture
composites.
Our
results
indicate
leads
phosphorylation
β-catenin
CREB
COX-2
expression.
believe
feasible
rapid
various
cell-based
regeneration
therapies.
Graphical
Язык: Английский
Clinical outcome is unlinked to injection of adipose-derived regenerative cells in the axilla of breast cancer-related lymphedema patients
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Ноя. 14, 2024
Injection
of
autologous
adipose-derived
regenerative
cells
(ADRCs)
combined
with
lipotransfer
has
been
suggested
to
alleviate
symptoms
in
diseases
including
breast
cancer-related
lymphedema
(BCRL).
We
recently
performed
a
randomized
controlled
trial
injecting
lipoaspirate
ADRCs
into
the
axilla
BCRL
patients,
and
here
we
aimed
intervention
group
define
an
unbiased
fashion
whether
ADRC
injection
was
linked
clinical
outcome.
39
patients
received
assisted
(4.20
×
107
±
1.75
cells)
whereas
41
were
included
for
placebo
treatment.
At
12
month
follow-up,
assessed
quality
life,
lymphangiography,
bioimpedance
enclosing
59
outcome
parameters.
Multifactorial
analysis
outcomes
used
responders
non-responders
intervention,
collected
from
these
patient
groups
analyzed
by
single
cell
RNA
sequencing
(scRNAseq).
Unbiased
multifactorial
ranked
defined
(Sf36
physical
change,
L-Dex
Lymph
Change,
ICG
mdanderson
change)
highest
effect
on
patients.
The
10
highest-
lowest
(five
five
non-responders)
study.
No
difference
between
observed
injected
number/size/viability
(p
>
0.05).
In
scRNAseq,
did
not
find
any
major
0.05)
composition
regarding
adipose
derived
stem
cells,
endothelial-,
smooth
muscle-,
T-,
B-,
mast
as
well
macrophages,
which
verified
flow
cytometry.
Differential
subcluster
gene
expression
92.5%
genes,
those
encoding
secretory
proteins,
below
threshold
1.5,
thus
neglible.
Together
this
that
phenotype
indistinguishable
intervention.
Our
data
suggest
or
heterogeneity
have
no
BCRL,
lipotranfer
should
therefore
be
considered
currently.
Язык: Английский
Dissecting human adipose tissue heterogeneity using single‐cell omics technologies
Stem Cell Research & Therapy,
Год журнала:
2024,
Номер
15(1)
Опубликована: Сен. 27, 2024
Язык: Английский
Clinical outcomes of autologous adipose-derived mesenchymal stem cell combined with high tibial osteotomy for knee osteoarthritis are correlated with stem cell stemness and senescence
Journal of Translational Medicine,
Год журнала:
2024,
Номер
22(1)
Опубликована: Ноя. 18, 2024
Mesenchymal
stem
cells
(MSCs)
have
been
proposed
to
treat
osteoarthritis
(OA)
for
many
years.
However,
clinical
outcomes
inconsistent
due
biological
variation
between
patients,
differences
in
tissue
source
and
preparation
of
the
MSCs,
type
donor
(e.g.
allogenic
versus
autologous).
Here,
we
test
hypothesis
that
are
related
variations
stemness
senescence
injected
autologous
adipose-derived
(AD)
MSCs.
In
prospective
randomized
trial,
45
knee
OA
patients
were
divided
into
two
groups:
Group
1
(n
=
22)
treated
with
high
tibial
osteotomy
(HTO)
alone
2
23)
HTO
followed
by
intra-articular
injection
AD-MSCs
(HTO
+
AD-MSCs).
MRI
X-ray
performed
pre-operation
12
months
post-operation.
WOMAC
VAS
score
collected
four
times,
every
6
over
a
24-month
follow-up.
We
observed
proliferation
selected
from
5
showing
most
improvement
least
improvement,
completed
further
vitro
experiments
including
beta-galactosidase
activity,
reactive
oxygen
species
bioinformatic
analysis.
The
results
showed
had
significant
reduction
severity
as
compared
alone.
Moreover,
discovered
colony
forming
efficiency
significantly
better
than
improvement.
also
lower
amounts
senescent
intracellular
species.
Clinical
therapy
correlated
cell
senescence.
Our
study
highlights
emerging
opportunities
trends
precision
medicine
could
potentially
improve
MSC-based
therapies.
Язык: Английский
Technological Advances in Adipose Tissue Research and Clinical Practice
Contemporary Endocrinology,
Год журнала:
2024,
Номер
unknown, С. 189 - 208
Опубликована: Янв. 1, 2024
Язык: Английский
Local Application of Minimally Manipulated Autologous Stromal Vascular Fraction (SVF) Reduces Inflammation and Improves Bilio-Biliary Anastomosis Integrity
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 222 - 222
Опубликована: Дек. 30, 2024
Bilio-biliary
anastomosis
(BBA)
is
a
critical
surgical
procedure
that
performed
with
the
objective
of
restoring
bile
duct
continuity.
This
often
required
in
cases
where
there
has
been
an
injury
to
extrahepatic
ducts
or
during
liver
transplantation.
Despite
advances
techniques,
healing
BBA
remains
significant
challenge,
complications
such
as
stricture
formation
and
leakage
affecting
patient
outcomes.
The
stromal
vascular
fraction
(SVF),
heterogeneous
cell
population
derived
from
adipose
tissue,
demonstrated
promise
regenerative
medicine
due
its
rich
content
stem
cells,
endothelial
progenitor
growth
factors.
this
study
was
evaluate
potential
locally
administered
autologous
SVF
enhance
BBAs.
on
swine
model
(female
Landrace
pigs).
Six
were
divided
into
two
groups:
treatment
group
(
Язык: Английский