Obesity
is
a
highly
prevalent
chronic
disease
characterized
by
excessive
weight
gain
and
fat
accumulation.
There
growing
evidence
that
Lactiplantibacillus
plantarum
strains
with
bile
salt
hydrolase
(BSH)
activity
are
effective
in
preventing
alleviating
obesity.
However,
few
of
them
has
confirmed
the
anti-obesity
effect
BSH
gene
deletion.
In
this
study,
23
BSH-positive
Lp.
were
screened
precipitation
halo
assay.
Among
these
strains,
Lp20
showed
highest
hydrolysis
rate
to
glycodeoxycholic
acid
(GDCA)
91.62%.
Genomic
sequencing
bioinformatics
analysis
indicated
bsh1
was
associated
deletion
role
Bsh1
GDCA
hydrolysis.
To
investigate
if
from
could
alleviate
obesity,
wild
type
mutant
administered
daily
for
8
weeks
diet-induced
obese
mice,
respectively.
Compared
strain,
strain
significantly
reduced
body
gain,
inguinal
white
adipose
tissue
mesenteric
mass
mice.
addition,
also
serum
total
cholesterol
levels
LDL-C
20.8%
33.3%,
Furthermore,
recovered
liver
steatosis
only
observed
treated
Taken
together,
key
functional
factor
on
ameliorating
HFD-induced
obesity
reducing
suppressing
adipogenesis
Frontiers in Microbiology,
Год журнала:
2022,
Номер
13
Опубликована: Май 9, 2022
The
lack
of
rapid
and
effective
approaches
to
determine
the
health
benefits
strains
is
one
main
challenges
affecting
selection
probiotics
from
large
numbers
candidates.
In
this
study,
probiotic
potential
44
Lactiplantibacillus
plantarum
isolated
different
Chinese
traditional
fermented
foods
was
evaluated,
including
acid
bile
salt
resistance,
adhesion
ability,
survival
in
simulated
human
gastrointestinal
transit,
antioxidant
activity,
hydrolase
(BSH)
antibacterial
activity.
All
tested
L.
showed
high
capacity,
BSH
Among
strains,
B652,
C232,
D444,
E932
were
identified
as
best
comprehensive
performed
which
selected
for
whole-genome
sequencing,
order
provide
clear
information
identify
key
genes
responsible
functional
characteristics
vitro.
It
demonstrated
that
ability
survive
gastric
environment
found
be
closely
correlated
with
enzyme
encoding
genes,
cell-surface
protein-encoding
stress
response
respectively.
present
might
decide
their
performance
profile
evaluation.
outcome
study
could
support
development
a
novel
approach
screening
identification
probiotics.
npj Science of Food,
Год журнала:
2025,
Номер
9(1)
Опубликована: Фев. 11, 2025
The
Western
diet
(WD)
leads
to
hepatic
lipid
metabolism
disorders.
Previous
studies
have
shown
that
the
bile
salt
hydrolase
1
(bsh1)
gene
of
Lactobacillus
plantarum
AR113
attenuates
colitis
under
WD.
In
this
study,
we
preliminarily
explored
how
inflammatory
response
in
mice
on
Our
study
suggests
WD
abnormalities
and
dysbiosis
gut
microflora,
furthermore,
there
may
be
a
correlation
between
responses.
significantly
regulated
acid
liver
treated
by
Dextran
sulfate
sodium
(DSS),
affected
structure
mouse
intestinal
flora,
inhibited
expression
Sterol
regulatory
element-binding
protein
1C
(SREBP-1C)
P-NF-κB
p65
at
level,
thereby
attenuating
injury
phenotype.
However,
bsh1
knockout
strain
did
not
exhibit
above
function.
Frontiers in Microbiology,
Год журнала:
2025,
Номер
16
Опубликована: Фев. 12, 2025
Gut
microbiota
has
been
increasingly
linked
to
metabolic
health
and
diseases
over
the
past
few
decades.
Bile
acids
(BAs),
major
components
of
bile,
are
bidirectionally
intestinal
microbiota,
also
known
as
gut
microbiome-BA
axis.
microbiota-derived
bile
salt
hydrolase
(BSH,
EC
3.5.1.24),
which
catalyzes
“gateway”
reaction
in
a
wider
pathway
acid
modification,
not
only
shapes
landscape,
but
modulates
crosstalk
between
host
health.
Therefore,
microbial
BSHs
exhibit
potential
directly
or
indirectly
influence
physiologies,
have
considered
promising
targets
for
modulation
benefit
animal
human
However,
their
physiological
functions
bacterial
physiologies
still
controversial
clear.
In
this
review,
we
mainly
discuss
current
evidence
related
roles
that
played
health,
possible
underlying
mechanisms.
Meanwhile,
present
applications
BSH-producing
probiotics
various
fields.
Finally,
describe
several
important
questions
need
be
addressed
by
further
investigations.
A
detailed
exploration
significance
will
contribute
future
diagnostic
therapeutic
improving
Research Square (Research Square),
Год журнала:
2025,
Номер
unknown
Опубликована: Март 5, 2025
Abstract
This
study
aimed
to
investigate
the
subspecies
diversity
of
10
Lactiplantibacillus
plantarum
strains
isolated
from
various
fermented
foods
by
RAPD-PCR
analysis.
Moreover,
their
technological
features
such
as
growth
in
different
temperatures,
NaCl
concentration,
and
proteolytic
properties
were
evaluated.
The
probiotic,
antimicrobial,
antioxidant
L.
postbioptic
characteristics
compared
with
Lactobacillus
rhamnosus
GG
a
commercial
reference
strain.
Principal
Component
Analysis
(PCA)
was
used
group
according
responses
following
variables:
microbial
strains.
results
confirmed
In-vitro
assays
indicate
similarity
ten
Their
survival
under
exposure
low
pH
bile
salts
determined
around
41.12–81.6%
97.41-116.04%,
respectively.
evaluation
aggregation,
hydrophobicity
cells
demonstrated
similar
cell
surface
characteristics.
In
addition
more
simulated
gastrointestinal
conditions,
postbiotics
derived
KMC45
jug
cheese
exhibited
greater
antibacterial
activity
than
rhamnosus
Finally,
PCA
analysis
closest
strain
probiotic
proposed
new
candidate
for
functional
starter
culture.
International Journal of Molecular Sciences,
Год журнала:
2022,
Номер
23(21), С. 12852 - 12852
Опубликована: Окт. 25, 2022
Lactobacillus,
a
genus
of
lactic
acid
bacteria,
plays
crucial
function
in
food
production
preservation,
and
probiotics.
It
is
particularly
important
to
develop
new
Lactobacillus
strains
with
superior
performance
by
gene
editing.
Currently,
the
identification
its
functional
genes
mining
excellent
mainly
rely
on
traditional
homologous
recombination
technology.
CRISPR/Cas9-based
genome
editing
rapidly
developing
technology
recent
years.
has
been
widely
applied
mammalian
cells,
plants,
yeast,
other
eukaryotes,
but
less
prokaryotes,
especially
Lactobacillus.
Compared
strain
improvement
methods,
can
greatly
improve
accuracy
target
sites
achieve
traceless
modification.
The
obtained
this
may
even
be
more
efficient
than
random
mutation
methods.
This
review
examines
application
current
issues
as
well
development
trend
In
addition,
fundamental
mechanisms
are
also
presented
summarized.
PLoS ONE,
Год журнала:
2022,
Номер
17(10), С. e0273986 - e0273986
Опубликована: Окт. 3, 2022
Lactiplantibacillus
plantarum
PMO
08
has
been
used
as
a
probiotic
starter
culture
for
plant-based
fermented
beverages,
with
various
health-promoting
effects
such
cholesterol-lowering
and
anti-inflammatory
activities.
This
study
aimed
to
analyze
the
genome
sequence
of
Lp.
identify
its
safety
characteristics
at
genomic
level.
For
this,
complete
sequencing
was
conducted
investigate
genes
associated
risk
by
using
Pacbio
combined
Illumina
HiSeq.
bacterial
strain
one
circular
chromosome
3,247,789
bp
44.5%
G
+
C
content
two
plasmids
50,296
39.0%
19,592
40.5%
content.
Orthologous
average
nucleotide
identity
analysis
showed
that
belongs
group
99.14%
similarity
WCFS1.
No
deleterious
were
determined
in
virulence
factor
analysis,
no
hemolysin
activity
or
secondary
bile
salt
synthesis
detected
vitro
test.
In
case
antibiotic
resistance
resistant
ampicillin
test,
but
these
not
transferable.
addition,
same
carbohydrate
utilization
WCFS1,
except
mannopyranoside,
which
only
our
can
metabolize.
The
also
harbors
gene
inositol
monophosphatase
family
protein
related
phytate
hydrolysis
have
several
metabolizing
rich
plant
environment.
Furthermore,
involved
phenotypes
acid/bile
tolerance,
adhesion
ability,
oxidative
stress
response
analysis.
demonstrates
probiotic-related
(with
genes)
is
suitable
fermentation.
Foods,
Год журнала:
2022,
Номер
11(19), С. 3063 - 3063
Опубликована: Окт. 2, 2022
Lactobacilli
have
been
widely
concerned
for
decades.
Bacteria
of
the
genus
Lactobacillus
commonly
employed
in
fermented
food
to
improve
appearance,
smell,
and
taste
or
prolong
its
shelf-life.
They
comprise
261
species
(by
March
2020)
that
are
highly
diverse
at
phenotypic,
ecological,
genotypic
levels.
Some
strains
documented
be
essential
probiotics,
which
defined
as
a
group
living
microorganisms
beneficial
health
host
when
ingested
sufficiency.
However,
characterization,
high-density
fermentation,
production
directed
vat
set
(DVS)
starter
used
industry
not
systematically
reported.
This
paper
mainly
focuses
on
reviewing
functional
cultures
industry,
including
different
molecular
techniques
identification
strain
levels,
methods
evaluating
properties,
enhancing
their
performance
improving
cell
density
Lactobacilli,
DVS
strains.
Moreover,
this
review
further
discussed
existing
problems
future
development
prospects
industry.
The
viability
stability
gastrointestinal
environment
critical
challenges
industrial
scale.
new
equipment
technology
will
potential
large-scale
application,
example,
developing
low-temperature
spray
drying,
freezing
granulation
freeze-drying.
Marine Drugs,
Год журнала:
2022,
Номер
20(12), С. 781 - 781
Опубликована: Дек. 15, 2022
The
unicellular
green
microalga
Dunaliella
is
a
potential
source
of
wide
range
nutritionally
important
compounds
applicable
to
the
food
industry.
aim
this
study
was
assess
effect
salina
dried
biomass
on
growth
and
adherence
10
strains
Lactobacillus,
Lacticaseibacillus,
Bifidobacterium.
immunomodulatory,
antioxidant,
cytotoxic
effects
D.
human
peripheral
mononuclear
cells
simulated
intestinal
epithelial
cell
lines
Caco-2
HT-29
were
evaluated.
Furthermore,
hypocholesterolemic
microalgae
lipid
metabolism
in
rats
fed
high-fat
diet
analyzed.
addition
had
positive
nine
out
probiotics
promoted
three
bifidobacteria
lines.
antioxidant
immunomodulatory
properties
concentration-dependent.
inflammatory
cytokines
(TNF-α
IL-6)
significantly
increased
following
stimulation
at
lowest
concentration
(0.5%
w/v).
Eight
week
supplementation
hypercholesteromic
decreased
serum
concentrations
LDL-C,
VLDL,
IDL-B,
IDL-C.
not
models;
it
promotes
selected
bifidobacteria,
affords
effects,
its
diets
may
help
decrease
atherosclerosis
risk
factors.
