The bsh1 gene of Lactobacillus plantarum AR113 ameliorates liver injury in colitis mice

npj Science of Food, Journal Year: 2025, Volume and Issue: 9(1)

Published: Feb. 11, 2025

The Western diet (WD) leads to hepatic lipid metabolism disorders. Previous studies have shown that the bile salt hydrolase 1 (bsh1) gene of Lactobacillus plantarum AR113 attenuates colitis under WD. In this study, we preliminarily explored how inflammatory response in mice on Our study suggests WD abnormalities and dysbiosis gut microflora, furthermore, there may be a correlation between responses. significantly regulated acid liver treated by Dextran sulfate sodium (DSS), affected structure mouse intestinal flora, inhibited expression Sterol regulatory element-binding protein 1C (SREBP-1C) P-NF-κB p65 at level, thereby attenuating injury phenotype. However, bsh1 knockout strain did not exhibit above function.

Language: Английский

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Assessments of Probiotic Potentials of Lactiplantibacillus plantarum Strains Isolated From Chinese Traditional Fermented Food: Phenotypic and Genomic Analysis

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: May 9, 2022

The lack of rapid and effective approaches to determine the health benefits strains is one main challenges affecting selection probiotics from large numbers candidates. In this study, probiotic potential 44 Lactiplantibacillus plantarum isolated different Chinese traditional fermented foods was evaluated, including acid bile salt resistance, adhesion ability, survival in simulated human gastrointestinal transit, antioxidant activity, hydrolase (BSH) antibacterial activity. All tested L. showed high capacity, BSH Among strains, B652, C232, D444, E932 were identified as best comprehensive performed which selected for whole-genome sequencing, order provide clear information identify key genes responsible functional characteristics vitro. It demonstrated that ability survive gastric environment found be closely correlated with enzyme encoding genes, cell-surface protein-encoding stress response respectively. present might decide their performance profile evaluation. outcome study could support development a novel approach screening identification probiotics.

Language: Английский

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Bile salt hydrolase: The complexity behind its mechanism in relation to lowering-cholesterol lactobacilli probiotics

Journal of Functional Foods, Journal Year: 2024, Volume and Issue: 120, P. 106357 - 106357

Published: July 20, 2024

Language: Английский

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Comparison of probiotic potential, antimicrobial, antioxidant and technological characteristics of various Lactiplantibacillus plantarum strains isolated from fermented foods

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: March 5, 2025

This study aimed to investigate the subspecies diversity of 10 Lactiplantibacillus plantarum strains isolated from various fermented foods by RAPD-PCR analysis. Moreover, their technological features such as growth in different temperatures, NaCl concentration, and proteolytic properties were evaluated. The probiotic, antimicrobial, antioxidant L. postbioptic characteristics compared with Lactobacillus rhamnosus GG a commercial reference strain. Principal Component Analysis (PCA) was used group according responses following variables: microbial strains. results confirmed In-vitro assays indicate similarity ten Their survival under exposure low pH bile salts determined around 41.12–81.6% 97.41-116.04%, respectively. evaluation aggregation, hydrophobicity cells demonstrated similar cell surface characteristics. In addition more simulated gastrointestinal conditions, postbiotics derived KMC45 jug cheese exhibited greater antibacterial activity than rhamnosus Finally, PCA analysis closest strain probiotic proposed new candidate for functional starter culture.

Language: Английский

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Development and Applications of CRISPR/Cas9-Based Genome Editing in Lactobacillus

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(21), P. 12852 - 12852

Published: Oct. 25, 2022

Lactobacillus, a genus of lactic acid bacteria, plays crucial function in food production preservation, and probiotics. It is particularly important to develop new Lactobacillus strains with superior performance by gene editing. Currently, the identification its functional genes mining excellent mainly rely on traditional homologous recombination technology. CRISPR/Cas9-based genome editing rapidly developing technology recent years. has been widely applied mammalian cells, plants, yeast, other eukaryotes, but less prokaryotes, especially Lactobacillus. Compared strain improvement methods, can greatly improve accuracy target sites achieve traceless modification. The obtained this may even be more efficient than random mutation methods. This review examines application current issues as well development trend In addition, fundamental mechanisms are also presented summarized.

Language: Английский

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Characterization, High-Density Fermentation, and the Production of a Directed Vat Set Starter of Lactobacilli Used in the Food Industry: A Review

Foods, Journal Year: 2022, Volume and Issue: 11(19), P. 3063 - 3063

Published: Oct. 2, 2022

Lactobacilli have been widely concerned for decades. Bacteria of the genus Lactobacillus commonly employed in fermented food to improve appearance, smell, and taste or prolong its shelf-life. They comprise 261 species (by March 2020) that are highly diverse at phenotypic, ecological, genotypic levels. Some strains documented be essential probiotics, which defined as a group living microorganisms beneficial health host when ingested sufficiency. However, characterization, high-density fermentation, production directed vat set (DVS) starter used industry not systematically reported. This paper mainly focuses on reviewing functional cultures industry, including different molecular techniques identification strain levels, methods evaluating properties, enhancing their performance improving cell density Lactobacilli, DVS strains. Moreover, this review further discussed existing problems future development prospects industry. The viability stability gastrointestinal environment critical challenges industrial scale. new equipment technology will potential large-scale application, example, developing low-temperature spray drying, freezing granulation freeze-drying.

Language: Английский

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Functional Properties of Dunaliella salina and Its Positive Effect on Probiotics

Marine Drugs, Journal Year: 2022, Volume and Issue: 20(12), P. 781 - 781

Published: Dec. 15, 2022

The unicellular green microalga Dunaliella is a potential source of wide range nutritionally important compounds applicable to the food industry. aim this study was assess effect salina dried biomass on growth and adherence 10 strains Lactobacillus, Lacticaseibacillus, Bifidobacterium. immunomodulatory, antioxidant, cytotoxic effects D. human peripheral mononuclear cells simulated intestinal epithelial cell lines Caco-2 HT-29 were evaluated. Furthermore, hypocholesterolemic microalgae lipid metabolism in rats fed high-fat diet analyzed. addition had positive nine out probiotics promoted three bifidobacteria lines. antioxidant immunomodulatory properties concentration-dependent. inflammatory cytokines (TNF-α IL-6) significantly increased following stimulation at lowest concentration (0.5% w/v). Eight week supplementation hypercholesteromic decreased serum concentrations LDL-C, VLDL, IDL-B, IDL-C. not models; it promotes selected bifidobacteria, affords effects, its diets may help decrease atherosclerosis risk factors.

Language: Английский

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Integrated genome-based assessment of safety and probiotic characteristics of Lactiplantibacillus plantarum PMO 08 isolated from kimchi

PLoS ONE, Journal Year: 2022, Volume and Issue: 17(10), P. e0273986 - e0273986

Published: Oct. 3, 2022

Lactiplantibacillus plantarum PMO 08 has been used as a probiotic starter culture for plant-based fermented beverages, with various health-promoting effects such cholesterol-lowering and anti-inflammatory activities. This study aimed to analyze the genome sequence of Lp. identify its safety characteristics at genomic level. For this, complete sequencing was conducted investigate genes associated risk by using Pacbio combined Illumina HiSeq. bacterial strain one circular chromosome 3,247,789 bp 44.5% G + C content two plasmids 50,296 39.0% 19,592 40.5% content. Orthologous average nucleotide identity analysis showed that belongs group 99.14% similarity WCFS1. No deleterious were determined in virulence factor analysis, no hemolysin activity or secondary bile salt synthesis detected vitro test. In case antibiotic resistance resistant ampicillin test, but these not transferable. addition, same carbohydrate utilization WCFS1, except mannopyranoside, which only our can metabolize. The also harbors gene inositol monophosphatase family protein related phytate hydrolysis have several metabolizing rich plant environment. Furthermore, involved phenotypes acid/bile tolerance, adhesion ability, oxidative stress response analysis. demonstrates probiotic-related (with genes) is suitable fermentation.

Language: Английский

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Enzymatic activities of Lactiplantibacillus plantarum: technological and functional role in food processing and human nutrition

Food Bioscience, Journal Year: 2024, Volume and Issue: 61, P. 104944 - 104944

Published: Aug. 16, 2024

Language: Английский

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The Expression Levels of Genes Responsible for the Enzymatic Activity of Bile Salt Hydrolase (BSH) and the Relationship of Cholesterol Assimilation in L. plantarum and L. paracasei

Current Microbiology, Journal Year: 2023, Volume and Issue: 80(6)

Published: May 8, 2023

Language: Английский

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