Grain & Oil Science and Technology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 1, 2025
Язык: Английский
Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(19), С. 11185 - 11194
Опубликована: Апрель 30, 2024
Aspergillus flavus contamination in agriculture and food industries poses threats to human health, leading a requirement of safe effective method control fungal contamination. Chitosan-based nitrogen-containing derivatives have attracted much attention due their safety enhanced antimicrobial applications. Herein, new benzimidazole-grafted chitosan (BAC) was synthesized by linking the (CS) with simple benzimidazole compound, 2-benzimidazolepropionic acid (BA). The characterization BAC confirmed Fourier transform infrared (FTIR) spectroscopy nuclear magnetic resonance (1H 13C NMR). Then, efficiency against A. ACCC 32656 investigated terms spore germination, mycelial growth, aflatoxin production. showed better antifungal effect than CS BA. minimum inhibitory concentration (MIC) value 1.25 mg/mL for BAC, while highest solubility (16.0 mg/mL) or BA (4.0 could not completely inhibit growth flavus. Furthermore, results that inhibited germination elongation affecting ergosterol biosynthesis cell membrane integrity, permeabilization plasma leakage intracellular content. production also when treated BAC. These findings indicate benzimidazole-derived natural has potential be used as an ideal agent preservation.
Язык: Английский
Процитировано
19
Antibiotics, Год журнала: 2022, Номер 11(12), С. 1727 - 1727
Опубликована: Дек. 1, 2022
Aspergillus flavus is a facultative parasite that contaminates several important food crops at both the pre- and post-harvest stages. Moreover, it an opportunistic animal human pathogen causes aspergillosis diseases. A. also produces polyketide-derived carcinogenic mutagenic secondary metabolite aflatoxin, which negatively impacts global security threatens livestock health. Recently, plant-derived natural compounds essential oils (EOs) have shown great potential in combatting spoilage aflatoxin contamination. In this review, situ antifungal antiaflatoxigenic properties of EOs are discussed. The mechanisms through affect growth biosynthesis then reviewed. Indeed, involve physical, chemical, or biochemical changes to cell wall, membrane, mitochondria, related metabolic enzymes genes. Finally, future perspectives towards application protection novel agent development present review highlights protect agricultural commodities items from contamination, along with reducing threat
Язык: Английский
Процитировано
44
LWT, Год журнала: 2021, Номер 154, С. 112660 - 112660
Опубликована: Окт. 25, 2021
Perilla frutescens essential oil (PEO) has been widely used as a food preservative. This study aimed to clarify the mechanism of PEO against Aspergillus flavus (A. flavus) at protein level by using TMT proteomics. Marked changes in polysaccharides, proteins, and lipids A. were observed after treatment with PEO. Disordered energy metabolism damaged defense function occurred PEO-treated flavus. Proteomic analysis further manifested that inhibited growth blocking antioxidative process glycolysis pathway. Moreover, additional energy-producing pathways activated compensate for unbalanced treatment. Meanwhile, upregulation enzymes related RNA transcription translation might be protective response decreased content proteins caused These results suggest could potentially developed an alternative agent prevent fungal aflatoxin contaminations cereals
Язык: Английский
Процитировано
43
Journal of Fungi, Год журнала: 2022, Номер 8(4), С. 388 - 388
Опубликована: Апрель 11, 2022
A major citrus postharvest pathogen, Penicillium italicum (P. italicum), causes substantial economic losses in citrus. In this study, a citral nanoemulsion containing polymethoxylated flavonoids (PMFs), the antimicrobial compounds from citrus, was prepared. The antifungal activity and potential mechanisms of against P. were evaluated. results showed that growth effectively inhibited by nanoemulsion, with minimum inhibitory concentration (MIC) fungicidal (MFC) 62.5 250 mg L−1, respectively. significantly spore germination mycelial growth, it altered morphology italicum. addition, permeability cell membrane increased increasing concentrations, as evidenced rapid rise extracellular electric conductivity stronger red fluorescence mycelia (propidium iodide staining). Compared control, treatment induced decrease total lipid ergosterol contents cells 64.61% 60.58%, respectively, demonstrating integrity had been disrupted. indicated PMFs-loaded exerted disrupting permeability; such may be used fungicide substitute for preservation fruits.
Язык: Английский
Процитировано
38
International Journal of Food Microbiology, Год журнала: 2022, Номер 384, С. 109972 - 109972
Опубликована: Окт. 12, 2022
Язык: Английский
Процитировано
38
LWT, Год журнала: 2022, Номер 164, С. 113635 - 113635
Опубликована: Июнь 6, 2022
o-Vanillin is an effective plant-derived compound inhibiting the growth of Aspergillus flavus (A. flavus). In this study, o-vanillin disrupted cell membrane A. via hindering K+ transport across plasma membrane, decreasing content lipid, ergosterol and trehalose, inducing lipid peroxidation. The HOG pathway might be upregulated as glycerol increased. Moreover, significantly promoted oxidative stress, indicated by accumulation intracellular reactive oxygen species (ROS) decreased activities antioxidant enzymes (e.g. superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx)). Finally, transcriptomic analysis was conducted to quantify differentially expressed genes treated o-vanillin. Consistent with above phenotypic changes, involved in several biological pathways (ergosterol biosynthesis, osmotic stress response) were regulated Real-time quantitative PCR (QRT-PCR) results showed that almost all participating biosynthesis downregulated addition, can effectively suppress pathogenicity on peanut butter. This study provides a new evidence antifungal activity against membranes flavus, will promote application into preservation grains agricultural products.
Язык: Английский
Процитировано
37
Frontiers in Plant Science, Год журнала: 2023, Номер 13
Опубликована: Янв. 12, 2023
Copper (Cu) and cadmium (Cd) are common heavy metal pollutants. When Cd excessive Cu accumulate in plants, plant growth is reduced. Our previous study showed that Germin-like proteins (GLPs), which exist tandem on chromosomes, a class of soluble glycoproteins respond to stress. In this study, hydroponic cultures were carried out investigate the effect GLP tolerance accumulation rice. The results knockout single OsGLP8-2 gene or ten OsGLP genes (OsGLP8-2 OsGLP8-11) resulted similar sensitivity toxicity. subjected stress, glp8-2 glp8-(2-11) mutants displayed more sensitive phenotype based height, root length, dry biomass rice seedlings. Correspondingly, concentrations significantly higher than those wild-type (WT) OsGLP8-2-overexpressing line. However, cell wall was opposite. Furthermore, we determined lignin accumulation. overexpressing-OsGLP8-2 line had shoot walls WT, glp8-2, glp8-(2-11). expression synthesis SOD activity OsGLP8-2, Diaminobe-nzidine (DAB), propidium iodide (PI) staining, Malondialdehyde (MDA) content determination suggested involved metal-induced antioxidant defense findings clearly suggest OsGLPs participate responses stress by deposition capacity rice, may play major role repeat clusters chromosome 8 under conditions.
Язык: Английский
Процитировано
20
Pest Management Science, Год журнала: 2024, Номер 80(9), С. 4273 - 4285
Опубликована: Апрель 16, 2024
Abstract BACKGROUND To discover novel fungicide candidates, five series of norbornene hydrazide, bishydrazide, oxadiazole, carboxamide and acylthiourea derivatives ( 2a‐2t , 3a‐3f, 4a‐4f, 5a‐5f 7a‐7f ) were designed, synthesized assayed for their antifungal activity toward seven representative plant fungal pathogens. RESULTS In the in vitro assay, some title presented good against Botryosphaeria dothidea Sclerotinia sclerotiorum Fusarium graminearum . Especially, compound 2b exhibited best inhibitory B. with median effective concentration (EC 50 0.17 mg L −1 substantially stronger than those reference fungicides boscalid carbendazim. The vivo assay on apples revealed that had significant curative protective effects, both which superior to boscalid. preliminary mechanism study, was able injure surface morphology hyphae, destroy cell membrane integrity increase intracellular reactive oxygen species (ROS) level addition, could considerably inhibit laccase (IC 1.02 μM, much positive control cysteine = 35.50 μM). binding affinity interaction mode also confirmed by molecular docking. CONCLUSION This study a promising lead inhibitors as fungicidal agrochemicals, demonstrate anti‐ activity. © 2024 Society Chemical Industry.
Язык: Английский
Процитировано
8
Foods, Год журнала: 2025, Номер 14(4), С. 682 - 682
Опубликована: Фев. 17, 2025
This investigation evaluates the comparative efficacy of Cinnamomum burmannii leaf essential oil (YXYO) and its main active ingredients as a novel preservative to protect stored food commodities from fungal infestations, aflatoxin B1 (AFB1) contamination caused by Aspergillus flavus. Morphological observations utilizing SEM TEM revealed significant alterations in treated samples, alongside decrease ergosterol content dose-dependent disruption antioxidant system energy system. Transcriptomic analysis suggested that differentially expressed genes were predominantly associated with spore growth, cell wall, membrane, oxidative stress, metabolism, biosynthesis. Solid-phase microextraction-gas chromatography-mass spectrometry (SPME-GC-MS) identified ten YXYO, including borneol, α-terpineol, terpinen-4-ol, etc. Moreover, an effective inhibition A. flavus infection peanuts was observed application 30 μL/disc YXYO blend compounds.
Язык: Английский
Процитировано
1
Australasian Plant Pathology, Год журнала: 2025, Номер unknown
Опубликована: Фев. 19, 2025
Язык: Английский
Процитировано
1