Antimicrobial resistance of Pseudomonas aeruginosa: navigating clinical impacts, current resistance trends, and innovations in breaking therapies

Frontiers in Microbiology, Год журнала: 2024, Номер 15

Опубликована: Апрель 5, 2024

Pseudomonas aeruginosa , a Gram-negative bacterium, is recognized for its adaptability and opportunistic nature. It poses substantial challenge in clinical settings due to complicated antibiotic resistance mechanisms, biofilm formation, capacity persistent infections both animal human hosts. Recent studies revealed potential zoonotic transmission of P. between animals, the environment, populations which highlights awareness this microbe. Implementation One Health approach, underscores connection human, animal, environmental health, we aim offer comprehensive perspective on current landscape management. This review presents innovative strategies designed counteract infections. Traditional antibiotics, while effective many cases, are increasingly compromised by development multidrug-resistant strains. Non-antibiotic avenues, such as quorum sensing inhibition, phage therapy, nanoparticle-based treatments, emerging promising alternatives. However, their application encounters obstacles like cost, side effects, safety concerns. Effectively addressing necessitates research efforts, advancements development, comprehension host-pathogen interactions deal with resilient pathogen.

Язык: Английский

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Mutations in the efflux pump regulator MexZ shift tissue colonization by Pseudomonas aeruginosa to a state of antibiotic tolerance

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 22, 2024

Abstract Mutations in mexZ , encoding a negative regulator of the expression mexXY efflux pump genes, are frequently acquired by Pseudomonas aeruginosa at early stages lung infection. Although traditionally related to resistance first-line drug tobramycin, mutations associated with low-level aminoglycoside when determined laboratory, suggesting that their selection during infection may not be necessarily, or only, tobramycin therapy. Here, we show -mutated bacteria tend accumulate inside epithelial barrier human airway model, thus colonising epithelium while being protected against diverse antibiotics. This phenotype is mediated overexpression lecA quorum sensing-controlled gene, lectin involved P. tissue invasiveness. We find caused disrupted equilibrium between overproduced MexXY and another pump, MexAB, which extrudes sensing signals. Our results indicate affect sensing-regulated pathways, promoting invasiveness protecting from action antibiotics within patients, something unnoticeable using standard laboratory tests.

Язык: Английский

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Quorum Quenching: A Drug Discovery Approach Against Pseudomonas aeruginosa

Microbiological Research, Год журнала: 2022, Номер 264, С. 127173 - 127173

Опубликована: Авг. 23, 2022

Язык: Английский

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A review of chemical signaling pathways in the quorum sensing circuit of Pseudomonas aeruginosa

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 254, С. 127861 - 127861

Опубликована: Ноя. 7, 2023

Язык: Английский

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Microbiologically Influenced Corrosion in Stainless Steel by Pseudomonas aeruginosa: An Overview

Journal of Bio- and Tribo-Corrosion, Год журнала: 2024, Номер 10(1)

Опубликована: Янв. 31, 2024

Abstract The loss of materials due to corrosion is inevitable and has gained significant attention at various stages in the process industries, including petroleum, food processing units, tanneries. With detrimental effects occurring as a result microbial activity, research on methods keep them under control become essential. About 10% affliction metals non-metals activity. Microbiologically Influenced Corrosion (MIC) consequence contact, which are synergetic between bacteria metals. A typical marine electroactive bacterium shown produce severe MIC Pseudomonas aeruginosa . In maritime environment, that extremely corrosive stainless steel. However, little known about how P. causes Stainless steel beneficial material been employed applications its corrosion-resistant properties mechanical strength. property strongly resist owed presence chromium composition. forms passive film chromium-rich oxide aerobic conditions low temperatures, protects it from surroundings. This layer can be harmed by harsh weather conditions. activity their existence modify inorganic layers, raising dissolution levels detaching surface metal. review aims study explicated methodologies more within broader context metal–microbe interactions.

Язык: Английский

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Mutational background influencesP. aeruginosaciprofloxacin resistance evolution but preserves collateral sensitivity robustness

Proceedings of the National Academy of Sciences, Год журнала: 2022, Номер 119(15)

Опубликована: Апрель 6, 2022

Collateral sensitivity is an evolutionary trade-off whereby acquisition of the adaptive phenotype resistance to antibiotic leads nonadaptive increased susceptibility another. The feasibility harnessing such a design evolutionary-based approaches for treating bacterial infections has been studied using model strains. However, clinical application collateral requires its conservation among strains presenting different mutational backgrounds. Particularly relevant studying robustness already-antibiotic-resistant mutants when challenged with new antimicrobial, common situation in clinics that hardly addressed. We submitted set diverse Pseudomonas aeruginosa antibiotic-resistant short-term evolution presence antimicrobials. Ciprofloxacin selects clinically mutations preexisting resistant mutants, which gave rise same, robust, aztreonam and tobramycin. then experimentally determined alternation ciprofloxacin more efficient than ciprofloxacin–tobramycin driving extinction analyzed mutants. Also, we show combinations ciprofloxacin–aztreonam or are most effective strategies eliminating tested P. These findings support identification conserved patterns may guide evolution-based treat infections, including those due Besides, this example phenotypic convergence absence parallel that, beyond antibiotic-resistance field, could facilitate understanding processes, where selective forces giving new, not clearly phenotypes remain unclear.

Язык: Английский

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Preparation and characterization of antibacterial, antioxidant, and biocompatible p-coumaric acid modified quaternized chitosan nanoparticles

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 242, С. 125087 - 125087

Опубликована: Май 27, 2023

Язык: Английский

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Molluscs—A ticking microbial bomb

Frontiers in Microbiology, Год журнала: 2023, Номер 13

Опубликована: Янв. 9, 2023

Bivalve shellfish consumption (ark shells, clams, cockles, and oysters) has increased over the last decades. Following this trend, infectious disease outbreaks associated with their have been reported more frequently. Molluscs are a diverse group of organisms found wild farmed. They common on our tables, but unfortunately, despite great taste, they can also pose threat as potential vector for numerous species pathogenic microorganisms. Clams, in particular, might be filled pathogens because filter-feeding diet. This specific way feeding favors accumulation excessive amounts microorganisms like Vibrio spp., including cholerae V. parahaemolyticus, Pseudomonas aeruginosa, Escherichia coli, Arcobacter fecal coliforms, intestinal enterococci. The problems pathogen dissemination caused by exogenous bacteria many geographical regions quickly became an unwanted effect globalized food supply chains, global climate change, natural transmission dynamics. Moreover, some Shewanella high zoonotic potential, spreading worldwide along transport. These bacteria, contained food, responsible antibiotic-resistance genes to belonging human microbiota. Finally, end up wastewater, thus colonizing new areas, which enables them introduce (ARG) into environment extend existing spectrum ARGs already present local biomes. Foodborne require modern methods detection. Similarly, detecting is necessary prevent resistance environments, preventing future outbreaks, could threaten consumers workers processing industry.

Язык: Английский

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Identifying Novel Therapeutics for the Resistant Mutant “F533L” in PBP3 of Pseudomonas aeruginosa Using ML Techniques

ACS Omega, Год журнала: 2024, Номер 9(26), С. 28046 - 28060

Опубликована: Июнь 14, 2024

Pseudomonas aeruginosa (P. aeruginosa) is a highly infectious and antibiotic-resistant bacterium, which causes acute chronic nosocomial infections. P. exhibits multidrug resistance due to the emergence of resistant mutants. The bacterium takes advantage intrinsic acquired mechanisms resist almost every antibiotic. To overcome drug-resistance problem, there need develop effective drugs against Therefore, in this study, we selected F533L mutation PBP3 (penicillin-binding protein 3) because its important role β-lactam recognition. target mutation, screened 147 antibacterial compounds from PubChem through machine-learning model developed based on decision stump algorithm with 75.75% accuracy filtered out 55 compounds. Subsequently, compounds, 47 were their drug-like activity. These subjected virtual screening obtain binding affinity range all was −11.3 −4.6 kcal mol–1. top 10 examined according point. A molecular dynamic simulation 8 conducted understand stability containing mutated PBP3. Out 3 namely, macozinone, agent 71, 123, showed good validated by RMSD, RMSF, binding-free analysis. findings study revealed promising mutant Furthermore, developments these may pave way for novel therapeutic interventions.

Язык: Английский

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Evolution of Habitat-Dependent Antibiotic Resistance in Pseudomonas aeruginosa

Microbiology Spectrum, Год журнала: 2022, Номер 10(4)

Опубликована: Июнь 29, 2022

Predicting evolution toward antibiotic resistance (AR) and its associated trade-offs, such as collateral sensitivity, is important to design evolution-based strategies tackle AR. However, the effect of nutrients' availability on evolution, particularly those that can be found under in vivo infection conditions, has been barely addressed.

Язык: Английский

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