The MexJK Multidrug Efflux Pump Is Not Involved in Acquired or Intrinsic Antibiotic Resistance in Pseudomonas aeruginosa, but Modulates the Bacterial Quorum Sensing Response DOI Open Access
Rafael Amieva, Teresa Gil‐Gil, José Luis Martínez

et al.

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(14), P. 7492 - 7492

Published: July 6, 2022

Multidrug efflux pumps are critical elements in both intrinsic and acquired antibiotic resistance of bacterial populations. Consequently, most studies regarding these protein machineries focus on this specific phenotype. Nevertheless, different works show that participate other aspects physiology too. Herein, we study the Pseudomonas aeruginosa multidrug pump MexJK. Previous studies, using model strains lacking MexAB-OprM MexCD-OprJ pumps, support MexJK can extrude erythromycin, tetracycline, triclosan. However, results here reported indicate potential increased extrusion, a mutant overexpressing mexJK, does not alter antibiotics susceptibility wild-type genetic background where all remain functional. clear impact quorum sensing (QS) response, mainly Pqs-dependent QS regulation network expression Pqs-regulated virulence factors, was observed linked to mexJK overexpression. The production siderophore pyoverdine strongly depended level expression, suggesting might P. pyoverdine-dependent iron homeostasis. All all, presented current article functions as MexJK, go beyond modulate relevant physiology.

Language: Английский

Antimicrobial resistance of Pseudomonas aeruginosa: navigating clinical impacts, current resistance trends, and innovations in breaking therapies DOI Creative Commons
Ahmed Elfadadny, Rokaia F. Ragab, Maha Alharbi

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 5, 2024

Pseudomonas aeruginosa , a Gram-negative bacterium, is recognized for its adaptability and opportunistic nature. It poses substantial challenge in clinical settings due to complicated antibiotic resistance mechanisms, biofilm formation, capacity persistent infections both animal human hosts. Recent studies revealed potential zoonotic transmission of P. between animals, the environment, populations which highlights awareness this microbe. Implementation One Health approach, underscores connection human, animal, environmental health, we aim offer comprehensive perspective on current landscape management. This review presents innovative strategies designed counteract infections. Traditional antibiotics, while effective many cases, are increasingly compromised by development multidrug-resistant strains. Non-antibiotic avenues, such as quorum sensing inhibition, phage therapy, nanoparticle-based treatments, emerging promising alternatives. However, their application encounters obstacles like cost, side effects, safety concerns. Effectively addressing necessitates research efforts, advancements development, comprehension host-pathogen interactions deal with resilient pathogen.

Language: Английский

Citations

40
Quorum Quenching: A Drug Discovery Approach Against Pseudomonas aeruginosa DOI Creative Commons
Muzamil Ahmad Rather, Debanjan Saha, Shuvam Bhuyan

et al.

Microbiological Research, Journal Year: 2022, Volume and Issue: 264, P. 127173 - 127173

Published: Aug. 23, 2022

Language: Английский

Citations

49
A review of chemical signaling pathways in the quorum sensing circuit of Pseudomonas aeruginosa DOI
Kayeen Vadakkan, Ajit Kumar Ngangbam, Kuppusamy Sathishkumar

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 254, P. 127861 - 127861

Published: Nov. 7, 2023

Language: Английский

Citations

35
Mutations in the efflux pump regulator MexZ shift tissue colonization by Pseudomonas aeruginosa to a state of antibiotic tolerance DOI Creative Commons
Pablo Laborda, Signe Lolle, Sara Hernando‐Amado

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: March 22, 2024

Abstract Mutations in mexZ , encoding a negative regulator of the expression mexXY efflux pump genes, are frequently acquired by Pseudomonas aeruginosa at early stages lung infection. Although traditionally related to resistance first-line drug tobramycin, mutations associated with low-level aminoglycoside when determined laboratory, suggesting that their selection during infection may not be necessarily, or only, tobramycin therapy. Here, we show -mutated bacteria tend accumulate inside epithelial barrier human airway model, thus colonising epithelium while being protected against diverse antibiotics. This phenotype is mediated overexpression lecA quorum sensing-controlled gene, lectin involved P. tissue invasiveness. We find caused disrupted equilibrium between overproduced MexXY and another pump, MexAB, which extrudes sensing signals. Our results indicate affect sensing-regulated pathways, promoting invasiveness protecting from action antibiotics within patients, something unnoticeable using standard laboratory tests.

Language: Английский

Citations

15
Microbiologically Influenced Corrosion in Stainless Steel by Pseudomonas aeruginosa: An Overview DOI Creative Commons

Koena Maji,

M. Lavanya

Journal of Bio- and Tribo-Corrosion, Journal Year: 2024, Volume and Issue: 10(1)

Published: Jan. 31, 2024

Abstract The loss of materials due to corrosion is inevitable and has gained significant attention at various stages in the process industries, including petroleum, food processing units, tanneries. With detrimental effects occurring as a result microbial activity, research on methods keep them under control become essential. About 10% affliction metals non-metals activity. Microbiologically Influenced Corrosion (MIC) consequence contact, which are synergetic between bacteria metals. A typical marine electroactive bacterium shown produce severe MIC Pseudomonas aeruginosa . In maritime environment, that extremely corrosive stainless steel. However, little known about how P. causes Stainless steel beneficial material been employed applications its corrosion-resistant properties mechanical strength. property strongly resist owed presence chromium composition. forms passive film chromium-rich oxide aerobic conditions low temperatures, protects it from surroundings. This layer can be harmed by harsh weather conditions. activity their existence modify inorganic layers, raising dissolution levels detaching surface metal. review aims study explicated methodologies more within broader context metal–microbe interactions.

Language: Английский

Citations

11
Mutational background influencesP. aeruginosaciprofloxacin resistance evolution but preserves collateral sensitivity robustness DOI Creative Commons
Sara Hernando‐Amado, Pablo Laborda, Jósé R. Valverde

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2022, Volume and Issue: 119(15)

Published: April 6, 2022

Collateral sensitivity is an evolutionary trade-off whereby acquisition of the adaptive phenotype resistance to antibiotic leads nonadaptive increased susceptibility another. The feasibility harnessing such a design evolutionary-based approaches for treating bacterial infections has been studied using model strains. However, clinical application collateral requires its conservation among strains presenting different mutational backgrounds. Particularly relevant studying robustness already-antibiotic-resistant mutants when challenged with new antimicrobial, common situation in clinics that hardly addressed. We submitted set diverse Pseudomonas aeruginosa antibiotic-resistant short-term evolution presence antimicrobials. Ciprofloxacin selects clinically mutations preexisting resistant mutants, which gave rise same, robust, aztreonam and tobramycin. then experimentally determined alternation ciprofloxacin more efficient than ciprofloxacin–tobramycin driving extinction analyzed mutants. Also, we show combinations ciprofloxacin–aztreonam or are most effective strategies eliminating tested P. These findings support identification conserved patterns may guide evolution-based treat infections, including those due Besides, this example phenotypic convergence absence parallel that, beyond antibiotic-resistance field, could facilitate understanding processes, where selective forces giving new, not clearly phenotypes remain unclear.

Language: Английский

Citations

29
Preparation and characterization of antibacterial, antioxidant, and biocompatible p-coumaric acid modified quaternized chitosan nanoparticles DOI
Boyuan Li,

Guozhu Chang,

Qifeng Dang

et al.

International Journal of Biological Macromolecules, Journal Year: 2023, Volume and Issue: 242, P. 125087 - 125087

Published: May 27, 2023

Language: Английский

Citations

15
Molluscs—A ticking microbial bomb DOI Creative Commons
Agnieszka Kijewska, Aleksandra Koroza, Katarzyna Grudlewska‐Buda

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Jan. 9, 2023

Bivalve shellfish consumption (ark shells, clams, cockles, and oysters) has increased over the last decades. Following this trend, infectious disease outbreaks associated with their have been reported more frequently. Molluscs are a diverse group of organisms found wild farmed. They common on our tables, but unfortunately, despite great taste, they can also pose threat as potential vector for numerous species pathogenic microorganisms. Clams, in particular, might be filled pathogens because filter-feeding diet. This specific way feeding favors accumulation excessive amounts microorganisms like Vibrio spp., including cholerae V. parahaemolyticus, Pseudomonas aeruginosa, Escherichia coli, Arcobacter fecal coliforms, intestinal enterococci. The problems pathogen dissemination caused by exogenous bacteria many geographical regions quickly became an unwanted effect globalized food supply chains, global climate change, natural transmission dynamics. Moreover, some Shewanella high zoonotic potential, spreading worldwide along transport. These bacteria, contained food, responsible antibiotic-resistance genes to belonging human microbiota. Finally, end up wastewater, thus colonizing new areas, which enables them introduce (ARG) into environment extend existing spectrum ARGs already present local biomes. Foodborne require modern methods detection. Similarly, detecting is necessary prevent resistance environments, preventing future outbreaks, could threaten consumers workers processing industry.

Language: Английский

Citations

13
Evolution of Habitat-Dependent Antibiotic Resistance in Pseudomonas aeruginosa DOI Creative Commons
Pablo Laborda, José Luis Martínez, Sara Hernando‐Amado

et al.

Microbiology Spectrum, Journal Year: 2022, Volume and Issue: 10(4)

Published: June 29, 2022

Predicting evolution toward antibiotic resistance (AR) and its associated trade-offs, such as collateral sensitivity, is important to design evolution-based strategies tackle AR. However, the effect of nutrients' availability on evolution, particularly those that can be found under in vivo infection conditions, has been barely addressed.

Language: Английский

Citations

21
In Silico Identification of Lead Compounds for Pseudomonas Aeruginosa PqsA Enzyme: Computational Study to Block Biofilm Formation DOI Creative Commons
Muhammad Shahab,

Muhammad Danial,

Taimur Khan

et al.

Biomedicines, Journal Year: 2023, Volume and Issue: 11(3), P. 961 - 961

Published: March 21, 2023

is an opportunistic Gram-negative bacterium implicated in acute and chronic nosocomial infections a leading cause of patient mortality.

Language: Английский

Citations

12