The efficacy of antimicrobial therapies in the treatment of mixed biofilms formed between Candida albicans and Porphyromonas gingivalis during epithelial cell infection in the aspiration pneumonia model

Medical Microbiology and Immunology, Год журнала: 2025, Номер 214(1)

Опубликована: Фев. 4, 2025

Abstract Aspiration pneumonia is a serious respiratory condition, which particularly prevalent in patients with dysphagia, neurological disorders, or those undergoing surgical interventions. The formation of multispecies biofilms the oral cavity, involving bacterial periodontopathogen Porphyromonas gingivalis and opportunistic pathogenic fungus Candida albicans , may also be related to development this disease, contributing resistance standard antimicrobial treatment. Therefore, research aimed evaluate efficacy selected antibiotics‒levofloxacin, metronidazole, meropenem, vancomycin‒and antifungal agents‒amphotericin B, caspofungin, fluconazole‒on these mixed aspiration model. While metronidazole levofloxacin effectively inhibited viability biofilms, lower doses increased release proteases. In conditions meropenem vancomycin showed reduced efficacy, requiring significantly higher achieve similar effect as single cultures. Treatment antifungals revealed that amphotericin B impacted fungal cell within was enhanced when drug applied presence P. . Caspofungin fluconazole variable caspofungin being more effective against C. cells biofilm. These findings indicated due mutual microbial protection mixed-species biofilm, retained its virulence despite increasing antibiotic doses. However, no excessive benefit observed for antifungals, indicating minor importance yeasts their protective role other pathogens infection. Graphical abstract

Язык: Английский

---

Silver vanadate nanomaterial incorporated into heat-cured resin and coating in printed resin - Antimicrobial activity in two multi-species biofilms and wettability

Journal of Dentistry, Год журнала: 2024, Номер 145, С. 104984 - 104984

Опубликована: Апрель 6, 2024

Язык: Английский

---

The role of FoxA, FiuA, and FpvB in iron acquisition via hydroxamate-type siderophores in Pseudomonas aeruginosa

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Авг. 13, 2024

Siderophores are specialized molecules produced by bacteria and fungi to scavenge iron, a crucial nutrient for growth metabolism. Catecholate-type siderophores mainly bacteria, while hydroxamates mostly from fungi. This study investigates the capacity of nine hydroxamate-type Streptomyces facilitate iron acquisition human pathogen Pseudomonas aeruginosa. Growth assays under limitation 55Fe incorporation tests showed that all promoted bacterial transport. The also aimed identify TonB-dependent transporters (TBDTs) involved in import these siderophores. Using mutant strains lacking specific TBDT genes, it was found is imported into P. aeruginosa cells FpvB coprogen, triacetylfusarinine, fusigen, ferrirhodin, ferrirubin. Iron complexed desferioxamine G transported FoxA, ferricrocin-Fe ferrichrycin-Fe FiuA, rhodotoluric acid-Fe FpvB, another unidentified TBDT. These findings highlight effectiveness transport provide insights complex molecular mechanisms involved, which important understanding microbial interactions ecological balance.

Язык: Английский

---

Efficacy of different microbial synthesized silver nanoparticles alone and in combination in single- and multi-species of Candida albicans and Pseudomonas aeruginosa biofilm inhibition and in downregulation of the HWP1 and PELA gene expression

Materials Today Communications, Год журнала: 2025, Номер 42, С. 111507 - 111507

Опубликована: Янв. 1, 2025

Язык: Английский

---

Interdigital and Plantar Foot Infections: A Retrospective Analysis of Molecularly Diagnosed Specimens in the United States and a Literature Review

Microorganisms, Год журнала: 2025, Номер 13(1), С. 184 - 184

Опубликована: Янв. 16, 2025

Up to one-quarter of the United States population is affected by tinea pedis (athlete's foot). Tinea web space (interdigital pedis) a common clinical presentation causing skin macerations and fissures. A "dermatophytosis complex" (i.e., concomitant bacterial colonization) further complicates treatment. Here, we examined records 14,429 specimens taken from feet dermatology podiatry outpatients over 4.6-year period; all were subjected multiplex qPCR diagnosis for detection dermatophytes, Candida, Corynebacterium minutissimum, Pseudomonas Staphylococcus aureus. literature search was conducted review reported prevalence fungal agents. In both interdigital plantar foot specimens, dermatophytes (33.3-33.8%) S. aureus (24.3-25%) found be predominate pathogens. space, higher C. minutissimum (15.7% vs. 7.9%) (23.5% 9.6%) found. The more likely observed in presence reflecting risk mixed infection. dermatophyte-positive variant at 45.5% (SD: 2.3). An analysis patient characteristics showed male patients exhibiting likelihoods dermatophyte, detections. elderly disproportionately infected with Candida. children, an common, which could attributed impetigo. recent lacks reporting on colonization patients, due reliance culture supplemented antibiotics. Geographical variation has been identified Trichophyton mentagrophytes complex. conclusion, PCR serves as valuable tool management pedis. accurate timely pathogens can better inform healthcare providers appropriate treatment selection.

Язык: Английский

---

Who arrived first? Priority effects on Candida albicans and Pseudomonas aeruginosa dual biofilms

Communications Biology, Год журнала: 2025, Номер 8(1)

Опубликована: Фев. 3, 2025

Historical processes in community assembly, such as species arrival order, influence interactions, causing priority effects. Candida albicans and Pseudomonas aeruginosa often co-occur biofilm-based infections of the skin, lungs, medical devices. Their predominantly antagonistic relationship involves complex physical chemical interactions. However, presence implications effects among these microorganisms remain largely unexplored. Here, we investigate impact effect dual-species biofilms using clinical isolates. By varying inoculation observe significant changes biofilm composition, structure, virulence, antimicrobial susceptibility. The first colonizer has an advantage for surface colonization. Consecutive colonization increases virulence negates C. albicans' protective on P. PAET1 against meropenem treatment. Finally, propose N-acetylcysteine adjuvant treating interkingdom infections, working independently Polymicrobial demonstrated that effects, influenced by species' affect

Язык: Английский

---

Lung Microenvironment Among Patients with Nontuberculous Mycobacterial Pulmonary Disease by Metagenomic Sequencing Technique

Biomedicines, Год журнала: 2025, Номер 13(4), С. 818 - 818

Опубликована: Март 28, 2025

Background: Nontuberculous mycobacterial pulmonary disease (NTM-PD) is an increasingly prevalent chronic infection, where the host immune status plays a crucial role in susceptibility and progression. The complex microenvironment, characterized by diverse microbial communities interactions, exhibits distinct features that may be fundamentally altered patient’s underlying state. Methods: A total of 111 sputum specimens 64 bronchoalveolar lavage fluid (BALF) were collected from 143 patients diagnosed with NTM-PD under different states. Metagenomic sequencing was performed on these to characterize compare microenvironmental among through comprehensive bioinformatic analyses. Results: immunosuppressed group exhibited lower α-diversity (p < 0.05). Principal Coordinates Analysis (PCoA) β-diversity for BALF revealed significant differences between groups Linear discriminant analysis Effect Size (LEfSe) identified species enriched group. co-occurrence network indicated had more structured actively connected networks compared control Mantel test confirmed abundance associated clinical immune–inflammation-related indicators patients. Conclusions: Our study reveals microenvironment NTM-PD. Further work required explore two-way relationship micro-organisms inflammatory responses, influence patient outcomes.

Язык: Английский

---

Antimicrobial activity of essential oils against biofilms formed in dental acrylic resin: a systematic review of in vitro studies

Biofouling, Год журнала: 2024, Номер 40(2), С. 114 - 129

Опубликована: Фев. 7, 2024

This study aimed to answer the question formulated according PICO strategy: 'Which essential oils show antimicrobial activity against biofilms formed on dental acrylic resin?' composed by population (dental resin), intervention (application of oils), comparison (denture cleansers, antifungal drugs, chlorhexidine, and oral mouthwashes), outcome (antibiofilm activity). In vitro experimental studies evaluating EOs biofilm resin were included. PRISMA guidelines followed, search was performed in PubMed, Science Direct, Embase, Lilacs databases gray literature using Google Scholar ProQuest December 2023. A manual reference lists included primary performed. Of 1467 articles identified, 37 selected for full-text reading 12 Twelve evaluated, which 11 showed Candida spp., 3 Staphylococcus aureus, 1 Pseudomonas aeruginosa. The Cymbopogon citratus, Cinnamomum zeylanicum, nardus higher action than C. Listerine, citratus nystatin, Melaleuca alternifolia fluconazole nystatin. However, chlorhexidine more effective Lippia sidoides Salvia officinalis, sodium hypochlorite L. sidoides, nystatin Zingiber officinale, Amphotericin B Eucalyptus globulus M. alternifolia. conclusion, nardus, reduce resin.

Язык: Английский

---

Structure-properties correlation of acrylic resins modified with silver vanadate and graphene

Heliyon, Год журнала: 2024, Номер 10(11), С. e32029 - e32029

Опубликована: Май 28, 2024

This study aimed to incorporate β-AgVO3 and rGO into self-curing (SC) heat-curing (HC) acrylic resins evaluate their physicochemical, mechanical, antimicrobial properties while correlating them with the characterized material structure. Acrylic resin samples were prepared at 0% (control), 0.5%, 1%, 3% for both nanoparticles. The microstructural characterization was assessed by scanning electron microscopy (SEM) (n=1) energy dispersive X-ray spectroscopy (EDS) (n=1). physicochemical mechanical tests included flexural strength (n=10), Knoop hardness roughness wettability sorption solubility porosity color evaluation (n=10). microbiological performed counting colony-forming units (CFU/mL) cell viability (n=8). results showed that lower counts of Candida albicans, Pseudomonas aeruginosa, Streptococcus mutans due promising properties. maintained addition β-AgVO3. higher microorganisms increase in It can be concluded incorporation could an alternative improve efficacy performance material.

Язык: Английский

---

Synergistic effects of quorum-sensing molecules and antimicrobials against Candida albicans and Pseudomonas aeruginosa biofilms: in vitro and in vivo studies

Journal of Antimicrobial Chemotherapy, Год журнала: 2024, Номер unknown

Опубликована: Авг. 30, 2024

Candida albicans can form polymicrobial biofilms with other microorganisms, such as Pseudomonas aeruginosa, at infection sites.

Язык: Английский

---