Impact of Antibiotics on the Genomic Expression ofPseudomonas aeruginosain the East African Community: A Systematic Review DOI Creative Commons
Comfort Danchal Vandu, Ilemobayo Victor Fasogbon, A. B. Agbaje

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 26, 2024

Abstract Antimicrobial resistance (AMR) presents a significant health problem globally with the majority of burden coming from lower-middle-income countries. AMR surveillance under One Health paradigm is critical for determining relationships between clinical, animal, and environmental levels. Allowing thorough knowledge interconnected variables contributing to resistance, which enables development effective solutions. This systematic review was conducted determine impact antibiotics on gene expression Pseudomonas spp. In East African Community. A comprehensive literature search across Web Science, Scopus, PubMed databases yielding 284 articles 11 meeting inclusion criteria after screening. We included studies 5 Countries that are part Community, results revealed high prevalence antimicrobial in aeruginosa , rates above 90% most tested antibiotics, exception Amikacin, remained due its limited use. Common genes reported carbapenem-resistant like blaNDM-1 blaVIM common method used disc diffusion at (50%). The also found high-risk clones, such as ST 244 357, were associated multidrug-resistant strains. Environmental isolates showed lower (54%) than clinical pathogens (73%), indicating different selecting pressures. Majority Kenya (30%) Uganda (30%), differences research capabilities healthcare facilities. These findings highlight need more surveillance, stewardship programs, additional prevent antibiotic guide public initiatives region. KEY FINDINGS OF THE STUDY demonstrated substantial including cefepime, meropenem, levofloxacin, ticarcillin-clavulanic acid various Africa. Amikacin be Africa potential treatment choice infections Carbapenem-resistant blaNDM-1, blaOXA-48 large number isolates. High-risk 357 demonstrate clonal spread settings. popular susceptibility testing (50%), owing low cost simplicity. DNA extraction PCR 30% whereas advanced approaches whole genome sequencing less resource constraints. undertaken fewer Tanzania Democratic Republic Congo (20%), demonstrating regional variations capacity resources.

Язык: Английский

Leveraging bacteria-inspired nanomaterials for targeted controlling biofilm and virulence properties of Pseudomonas aeruginosa DOI
Palanivel Sathishkumar, Fazlurrahman Khan

Microbial Pathogenesis, Год журнала: 2024, Номер 197, С. 107103 - 107103

Опубликована: Ноя. 5, 2024

Язык: Английский

Процитировано

4

TAS2R38 genotype and CRS severity in children with cystic fibrosis DOI Creative Commons
Elena Cantone, Antonella Tosco, Angela Sepe

и другие.

Heliyon, Год журнала: 2025, Номер 11(1), С. e41716 - e41716

Опубликована: Янв. 1, 2025

BackgroundCystic fibrosis is a heterogeneous disease whose severity and symptoms largely depend on the functional impact of mutations in cystic transmembrane conductance regulator gene. Other genes may also modulate clinical manifestations complications associated with fibrosis. Genetic variants bitter taste receptor TAS2R38 have been shown to contribute susceptibility chronic rhinosinusitis. This study aims elucidate role as novel modifier gene influencing sinonasal pulmonary Pseudomonas Aeruginosa colonization children fibrosis.MethodsThis retrospective observational case-control evaluated sinus features, quality life, occurrence 69 Propylthiouracil testing genotyping were performed characterize patients based functionality.ResultsThe non-taster genetic variant was greater rhinosinusitis, measured by endoscopic radiological scores, compared taster (p=0.031 p=0.03, respectively). Furthermore, an inverse correlation observed between age at first infection rhinosinusitis assessed score (r=-0.3388, p = 0.0302).ConclusionsThe findings highlight potential The implications include development T2R38-targeted topical therapies use or predict infection. In addition, these results pave way for novel, tailored therapeutic approaches era precision medicine.Graphical abstract

Язык: Английский

Процитировано

0

A Comprehensive Methodology for Identifying Pseudomonas aeruginosa Strains Exhibiting Biofilm and Virulence Factor Traits and Assessment of Biofilm Resistance Against Commercial Disinfectant DOI Creative Commons

Maha Guesmi,

M. Ben Hmida, Salma Smaoui

и другие.

Microbiology Research, Год журнала: 2025, Номер 16(3), С. 62 - 62

Опубликована: Март 9, 2025

In this study, biofilm formation and the production of key virulence factors were systematically evaluated across 33 strains Pseudomonas aeruginosa isolated from diverse hospital environments in Tunisia. Among these, 13 demonstrated strong biofilm-forming capacities. Adding glucose (9%, w/v) to culture medium generally enhanced development, indicating that supplementation may promote clinical isolates. The selected exhibited a consistent critical factors, including pyocyanin, rhamnolipid, LasA protease, known for its staphylolytic activity. However, profiles extracellular polysaccharide (EPS) motility showed considerable heterogeneity among strains, suggesting strain-specific regulation these traits. Based on comprehensive analysis expression, strain 2629 was chosen as model organism due robust biofilm-producing ability high factor output. This used subsequent vitro assays evaluate anti-biofilm potential commercial disinfectant containing peracetic acid other active agents. Results indicated 3% (v/v) concentration disinfectant, applied 5 min, nearly sufficient eradicate formed by strain. These findings underscore importance selecting representative accurate assessments

Язык: Английский

Процитировано

0

Repurposing High-Throughput Screening Reveals Unconventional Drugs with Antimicrobial and Antibiofilm Potential Against Methicillin-Resistant Staphylococcus aureus from a Cystic Fibrosis Patient DOI Creative Commons
Arianna Pompilio,

Veronica Lupetti,

Valentina Puca

и другие.

Antibiotics, Год журнала: 2025, Номер 14(4), С. 402 - 402

Опубликована: Апрель 14, 2025

Background/Objectives: Antibiotic therapy faces challenges from rising acquired and biofilm-related antibiotic resistance rates. High levels to commonly used antibiotics have been observed in methicillin-resistant Staphylococcus aureus (MRSA) strains among cystic fibrosis (CF) patients, indicating an urgent need for new antibacterial agents. This study aimed identify potential novel therapeutics with antibiofilm activities against MRSA CF strain by screening, the first time, Drug Repurposing Compound Library (MedChem Express). Methods/Results: Among 3386 compounds, a high-throughput screening-based spectrophotometric approach identified 2439 (72%), 654 (19.3%), 426 (12.6%) drugs active planktonic cells, biofilm formation, preformed biofilm, respectively, although different extents. The most hits were 193 (5.7%), causing 100% growth inhibition; 5 (0.14%), excellent activity formation (i.e., reduction ≥ 90%); 4, showing high 60% ≤ < 90%) biofilms. belonged several primary research areas, “cancer” being prevalent. After performing literature review other, already published biological properties that could be relevant lung environment other pathogens, anti-inflammatory anti-virulence potential), interesting following: 5-(N,N-Hexamethylene)-amiloride (diuretic), Toremifene (anticancer), Zafirlukast (antiasthmatic), Fenretide Montelukast (antiasthmatic) S. cells; Hemin formation; Heparin, Clemastine (antihistaminic), Bromfenac (nonsteroidal anti-inflammatory) established Conclusions: These findings warrant further vitro vivo studies confirm of repurposing these compounds managing infections caused patients.

Язык: Английский

Процитировано

0

FDA-approved drugs for targeting virulence of Pseudomonas aeruginosa: A drug repurposing approach to combat multidrug resistance DOI
Bharti Sharma, Jatin Chadha,

Lavanya Khullar

и другие.

Microbial Pathogenesis, Год журнала: 2025, Номер unknown, С. 107781 - 107781

Опубликована: Июнь 1, 2025

Язык: Английский

Процитировано

0

Revitalizing common drugs for antibacterial, quorum quenching, and antivirulence potential against Pseudomonas aeruginosa: in vitro and in silico insights DOI
Jatin Chadha,

Umang Mudgil,

Lavanya Khullar

и другие.

3 Biotech, Год журнала: 2024, Номер 14(10)

Опубликована: Сен. 4, 2024

Язык: Английский

Процитировано

0

Novel Polyphenolic Hybrids Modulate Quorum Sensing Regulated Virulence in Pseudomonas aeruginosa DOI

Tamanna Dua,

Jatin Chadha,

Akshita Goel

и другие.

ChemistrySelect, Год журнала: 2024, Номер 9(41)

Опубликована: Окт. 28, 2024

Abstract Naturally occurring substituted benzoic acids, known for their exceptional biological activity against Pseudomonas aeruginosa , were reacted with various aldehydes, aniline, and tert‐butyl isocyanide at 25 °C to give the desired hybrids good excellent yield in range of 60–85 % well characterized by 1 H NMR, 13 C HRMS, IR, HPLC analysis. Molecular docking simulation studies compounds conducted which predicted a high affinity towards quorum‐sensing (QS) receptors P. . The polyphenolic first screened anti‐QS using Agrobacterium tumefaciens NTL4 biosensor strain, followed examining antivirulence potential selected PAO1 vitro highest degree AHL (acyl homoserine lactone) inhibition was observed compound having maximum number phenolic groups. this investigation also found be non‐cytotoxic (biocompatible) HEK cells may further tested therapeutic efficacy infections vivo It has been that is directly proportional hydroxyl groups on aromatic ring one major contributing factors can chelating capacity as depicted CAS shuttle assay.

Язык: Английский

Процитировано

0

Impact of Antibiotics on the Genomic Expression ofPseudomonas aeruginosain the East African Community: A Systematic Review DOI Creative Commons
Comfort Danchal Vandu, Ilemobayo Victor Fasogbon, A. B. Agbaje

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 26, 2024

Abstract Antimicrobial resistance (AMR) presents a significant health problem globally with the majority of burden coming from lower-middle-income countries. AMR surveillance under One Health paradigm is critical for determining relationships between clinical, animal, and environmental levels. Allowing thorough knowledge interconnected variables contributing to resistance, which enables development effective solutions. This systematic review was conducted determine impact antibiotics on gene expression Pseudomonas spp. In East African Community. A comprehensive literature search across Web Science, Scopus, PubMed databases yielding 284 articles 11 meeting inclusion criteria after screening. We included studies 5 Countries that are part Community, results revealed high prevalence antimicrobial in aeruginosa , rates above 90% most tested antibiotics, exception Amikacin, remained due its limited use. Common genes reported carbapenem-resistant like blaNDM-1 blaVIM common method used disc diffusion at (50%). The also found high-risk clones, such as ST 244 357, were associated multidrug-resistant strains. Environmental isolates showed lower (54%) than clinical pathogens (73%), indicating different selecting pressures. Majority Kenya (30%) Uganda (30%), differences research capabilities healthcare facilities. These findings highlight need more surveillance, stewardship programs, additional prevent antibiotic guide public initiatives region. KEY FINDINGS OF THE STUDY demonstrated substantial including cefepime, meropenem, levofloxacin, ticarcillin-clavulanic acid various Africa. Amikacin be Africa potential treatment choice infections Carbapenem-resistant blaNDM-1, blaOXA-48 large number isolates. High-risk 357 demonstrate clonal spread settings. popular susceptibility testing (50%), owing low cost simplicity. DNA extraction PCR 30% whereas advanced approaches whole genome sequencing less resource constraints. undertaken fewer Tanzania Democratic Republic Congo (20%), demonstrating regional variations capacity resources.

Язык: Английский

Процитировано

0