Leveraging bacteria-inspired nanomaterials for targeted controlling biofilm and virulence properties of Pseudomonas aeruginosa
Microbial Pathogenesis,
Год журнала:
2024,
Номер
197, С. 107103 - 107103
Опубликована: Ноя. 5, 2024
Язык: Английский
TAS2R38 genotype and CRS severity in children with cystic fibrosis
Heliyon,
Год журнала:
2025,
Номер
11(1), С. e41716 - e41716
Опубликована: Янв. 1, 2025
BackgroundCystic
fibrosis
is
a
heterogeneous
disease
whose
severity
and
symptoms
largely
depend
on
the
functional
impact
of
mutations
in
cystic
transmembrane
conductance
regulator
gene.
Other
genes
may
also
modulate
clinical
manifestations
complications
associated
with
fibrosis.
Genetic
variants
bitter
taste
receptor
TAS2R38
have
been
shown
to
contribute
susceptibility
chronic
rhinosinusitis.
This
study
aims
elucidate
role
as
novel
modifier
gene
influencing
sinonasal
pulmonary
Pseudomonas
Aeruginosa
colonization
children
fibrosis.MethodsThis
retrospective
observational
case-control
evaluated
sinus
features,
quality
life,
occurrence
69
Propylthiouracil
testing
genotyping
were
performed
characterize
patients
based
functionality.ResultsThe
non-taster
genetic
variant
was
greater
rhinosinusitis,
measured
by
endoscopic
radiological
scores,
compared
taster
(p=0.031
p=0.03,
respectively).
Furthermore,
an
inverse
correlation
observed
between
age
at
first
infection
rhinosinusitis
assessed
score
(r=-0.3388,
p
=
0.0302).ConclusionsThe
findings
highlight
potential
The
implications
include
development
T2R38-targeted
topical
therapies
use
or
predict
infection.
In
addition,
these
results
pave
way
for
novel,
tailored
therapeutic
approaches
era
precision
medicine.Graphical
abstract
Язык: Английский
A Comprehensive Methodology for Identifying Pseudomonas aeruginosa Strains Exhibiting Biofilm and Virulence Factor Traits and Assessment of Biofilm Resistance Against Commercial Disinfectant
Microbiology Research,
Год журнала:
2025,
Номер
16(3), С. 62 - 62
Опубликована: Март 9, 2025
In
this
study,
biofilm
formation
and
the
production
of
key
virulence
factors
were
systematically
evaluated
across
33
strains
Pseudomonas
aeruginosa
isolated
from
diverse
hospital
environments
in
Tunisia.
Among
these,
13
demonstrated
strong
biofilm-forming
capacities.
Adding
glucose
(9%,
w/v)
to
culture
medium
generally
enhanced
development,
indicating
that
supplementation
may
promote
clinical
isolates.
The
selected
exhibited
a
consistent
critical
factors,
including
pyocyanin,
rhamnolipid,
LasA
protease,
known
for
its
staphylolytic
activity.
However,
profiles
extracellular
polysaccharide
(EPS)
motility
showed
considerable
heterogeneity
among
strains,
suggesting
strain-specific
regulation
these
traits.
Based
on
comprehensive
analysis
expression,
strain
2629
was
chosen
as
model
organism
due
robust
biofilm-producing
ability
high
factor
output.
This
used
subsequent
vitro
assays
evaluate
anti-biofilm
potential
commercial
disinfectant
containing
peracetic
acid
other
active
agents.
Results
indicated
3%
(v/v)
concentration
disinfectant,
applied
5
min,
nearly
sufficient
eradicate
formed
by
strain.
These
findings
underscore
importance
selecting
representative
accurate
assessments
Язык: Английский
Repurposing High-Throughput Screening Reveals Unconventional Drugs with Antimicrobial and Antibiofilm Potential Against Methicillin-Resistant Staphylococcus aureus from a Cystic Fibrosis Patient
Antibiotics,
Год журнала:
2025,
Номер
14(4), С. 402 - 402
Опубликована: Апрель 14, 2025
Background/Objectives:
Antibiotic
therapy
faces
challenges
from
rising
acquired
and
biofilm-related
antibiotic
resistance
rates.
High
levels
to
commonly
used
antibiotics
have
been
observed
in
methicillin-resistant
Staphylococcus
aureus
(MRSA)
strains
among
cystic
fibrosis
(CF)
patients,
indicating
an
urgent
need
for
new
antibacterial
agents.
This
study
aimed
identify
potential
novel
therapeutics
with
antibiofilm
activities
against
MRSA
CF
strain
by
screening,
the
first
time,
Drug
Repurposing
Compound
Library
(MedChem
Express).
Methods/Results:
Among
3386
compounds,
a
high-throughput
screening-based
spectrophotometric
approach
identified
2439
(72%),
654
(19.3%),
426
(12.6%)
drugs
active
planktonic
cells,
biofilm
formation,
preformed
biofilm,
respectively,
although
different
extents.
The
most
hits
were
193
(5.7%),
causing
100%
growth
inhibition;
5
(0.14%),
excellent
activity
formation
(i.e.,
reduction
≥
90%);
4,
showing
high
60%
≤
<
90%)
biofilms.
belonged
several
primary
research
areas,
“cancer”
being
prevalent.
After
performing
literature
review
other,
already
published
biological
properties
that
could
be
relevant
lung
environment
other
pathogens,
anti-inflammatory
anti-virulence
potential),
interesting
following:
5-(N,N-Hexamethylene)-amiloride
(diuretic),
Toremifene
(anticancer),
Zafirlukast
(antiasthmatic),
Fenretide
Montelukast
(antiasthmatic)
S.
cells;
Hemin
formation;
Heparin,
Clemastine
(antihistaminic),
Bromfenac
(nonsteroidal
anti-inflammatory)
established
Conclusions:
These
findings
warrant
further
vitro
vivo
studies
confirm
of
repurposing
these
compounds
managing
infections
caused
patients.
Язык: Английский
FDA-approved drugs for targeting virulence of Pseudomonas aeruginosa: A drug repurposing approach to combat multidrug resistance
Microbial Pathogenesis,
Год журнала:
2025,
Номер
unknown, С. 107781 - 107781
Опубликована: Июнь 1, 2025
Язык: Английский
Revitalizing common drugs for antibacterial, quorum quenching, and antivirulence potential against Pseudomonas aeruginosa: in vitro and in silico insights
3 Biotech,
Год журнала:
2024,
Номер
14(10)
Опубликована: Сен. 4, 2024
Язык: Английский
Novel Polyphenolic Hybrids Modulate Quorum Sensing Regulated Virulence in Pseudomonas aeruginosa
ChemistrySelect,
Год журнала:
2024,
Номер
9(41)
Опубликована: Окт. 28, 2024
Abstract
Naturally
occurring
substituted
benzoic
acids,
known
for
their
exceptional
biological
activity
against
Pseudomonas
aeruginosa
,
were
reacted
with
various
aldehydes,
aniline,
and
tert‐butyl
isocyanide
at
25
°C
to
give
the
desired
hybrids
good
excellent
yield
in
range
of
60–85
%
well
characterized
by
1
H
NMR,
13
C
HRMS,
IR,
HPLC
analysis.
Molecular
docking
simulation
studies
compounds
conducted
which
predicted
a
high
affinity
towards
quorum‐sensing
(QS)
receptors
P.
.
The
polyphenolic
first
screened
anti‐QS
using
Agrobacterium
tumefaciens
NTL4
biosensor
strain,
followed
examining
antivirulence
potential
selected
PAO1
vitro
highest
degree
AHL
(acyl
homoserine
lactone)
inhibition
was
observed
compound
having
maximum
number
phenolic
groups.
this
investigation
also
found
be
non‐cytotoxic
(biocompatible)
HEK
cells
may
further
tested
therapeutic
efficacy
infections
vivo
It
has
been
that
is
directly
proportional
hydroxyl
groups
on
aromatic
ring
one
major
contributing
factors
can
chelating
capacity
as
depicted
CAS
shuttle
assay.
Язык: Английский
Impact of Antibiotics on the Genomic Expression ofPseudomonas aeruginosain the East African Community: A Systematic Review
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 26, 2024
Abstract
Antimicrobial
resistance
(AMR)
presents
a
significant
health
problem
globally
with
the
majority
of
burden
coming
from
lower-middle-income
countries.
AMR
surveillance
under
One
Health
paradigm
is
critical
for
determining
relationships
between
clinical,
animal,
and
environmental
levels.
Allowing
thorough
knowledge
interconnected
variables
contributing
to
resistance,
which
enables
development
effective
solutions.
This
systematic
review
was
conducted
determine
impact
antibiotics
on
gene
expression
Pseudomonas
spp.
In
East
African
Community.
A
comprehensive
literature
search
across
Web
Science,
Scopus,
PubMed
databases
yielding
284
articles
11
meeting
inclusion
criteria
after
screening.
We
included
studies
5
Countries
that
are
part
Community,
results
revealed
high
prevalence
antimicrobial
in
aeruginosa
,
rates
above
90%
most
tested
antibiotics,
exception
Amikacin,
remained
due
its
limited
use.
Common
genes
reported
carbapenem-resistant
like
blaNDM-1
blaVIM
common
method
used
disc
diffusion
at
(50%).
The
also
found
high-risk
clones,
such
as
ST
244
357,
were
associated
multidrug-resistant
strains.
Environmental
isolates
showed
lower
(54%)
than
clinical
pathogens
(73%),
indicating
different
selecting
pressures.
Majority
Kenya
(30%)
Uganda
(30%),
differences
research
capabilities
healthcare
facilities.
These
findings
highlight
need
more
surveillance,
stewardship
programs,
additional
prevent
antibiotic
guide
public
initiatives
region.
KEY
FINDINGS
OF
THE
STUDY
demonstrated
substantial
including
cefepime,
meropenem,
levofloxacin,
ticarcillin-clavulanic
acid
various
Africa.
Amikacin
be
Africa
potential
treatment
choice
infections
Carbapenem-resistant
blaNDM-1,
blaOXA-48
large
number
isolates.
High-risk
357
demonstrate
clonal
spread
settings.
popular
susceptibility
testing
(50%),
owing
low
cost
simplicity.
DNA
extraction
PCR
30%
whereas
advanced
approaches
whole
genome
sequencing
less
resource
constraints.
undertaken
fewer
Tanzania
Democratic
Republic
Congo
(20%),
demonstrating
regional
variations
capacity
resources.
Язык: Английский