Unraveling the Mechanism of the Endophytic Bacterial Strain Pseudomonas oryzihabitans GDW1 in Enhancing Tomato Plant Growth Through Modulation of the Host Transcriptome and Bacteriome
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(5), С. 1922 - 1922
Опубликована: Фев. 23, 2025
Endophytic
Pseudomonas
species
from
agricultural
crops
have
been
extensively
studied
for
their
plant-growth-promoting
(PGP)
potential,
but
little
is
known
about
PGP
potential
when
isolated
perennial
trees.
This
study
investigated
the
of
an
endophyte,
oryzihabitans
GDW1,
a
healthy
pine
tree
by
taking
tomato
as
host
plant.
We
employed
multiomics
approaches
(transcriptome
and
bacteriome
analyses)
to
elucidate
underlying
mechanisms
GDW1.
The
results
greenhouse
experiments
revealed
that
application
GDW1
significantly
improved
plant
growth,
increasing
shoot
length,
root
fresh
weight,
biomass
accumulation
up
44%,
38%,
54%,
59%,
respectively,
compared
with
control.
Transcriptomic
analysis
1158
differentially
expressed
genes
enriched
in
hormone
signaling
(auxin,
gibberellin,
cytokinin)
stress
response
(plant-pathogen
interaction,
MAPK
pathway-plant,
phenylpropanoid
biosynthesis)
pathways.
Protein-protein
interaction
network
nine
hub
(MAPK10,
ARF19-1,
SlCKX1,
GA2ox2,
PAL5,
SlWRKY37,
GH3.6,
XTH3,
NML1)
related
tolerance,
control,
defense.
Analysis
through
16S
rRNA
gene
amplicon
sequencing
inoculation
dramatically
altered
bacterial
community
structure,
enhancing
diversity
abundance
beneficial
taxa
(Proteobacteria
Bacteroidota).
Co-occurrence
showed
complex
treated
plants,
suggesting
increasingly
intricate
microbial
relationships
nutrient
absorption.
Additionally,
FAPROTAX
PICRUSt2
functional
prediction
analyses
suggested
role
nitrogen
cycling,
organic
matter
degradation,
growth
promotion,
resistance.
In
conclusion,
this
provides
novel
insights
into
symbiotic
relationship
between
P.
highlighting
its
biofertilizer
sustainable
agriculture
means
reducing
reliance
on
agrochemicals.
Язык: Английский
Plant Growth-Promoting Microorganisms: new insights and the way forward
Microbiological Research,
Год журнала:
2025,
Номер
unknown, С. 128168 - 128168
Опубликована: Апрель 1, 2025
Язык: Английский
Integrated microbiome and metabolome approaches reveal the regulatory mechanism of pumpkin pollination
Jian Xiao,
Yu Zhu,
Lanjie Liao
и другие.
Microbiological Research,
Год журнала:
2025,
Номер
unknown, С. 128185 - 128185
Опубликована: Апрель 1, 2025
Язык: Английский
The accumulation of major active ingredients associated to mutualistic endophytic fungi in the stem of Ephedra sinica with different growth years
Symbiosis,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 16, 2025
Язык: Английский
Plant beneficial traits of endophytic bacteria associated with fennel (<i>Foeniculum vulgare</i> Mill.)
AIMS Microbiology,
Год журнала:
2024,
Номер
10(2), С. 449 - 467
Опубликована: Янв. 1, 2024
In
this
study,
we
used
16S
rRNA
gene
sequence
analysis
to
describe
the
diversity
of
cultivable
endophytic
bacteria
associated
with
fennel
(
Язык: Английский
Optimal Irrigation and Fertilization Enhanced Tomato Yield and Water and Nitrogen Productivities by Increasing Rhizosphere Microbial Nitrogen Fixation
Agronomy,
Год журнала:
2024,
Номер
14(9), С. 2111 - 2111
Опубликована: Сен. 17, 2024
Irrigation
and
nitrogen
application
rates
have
significant
effects
on
greenhouse
tomato
yields,
as
well
water
use
efficiencies,
but
little
is
known
regarding
how
these
affect
plant–microbiome
interactions
the
associated
changes
might
impact
yields.
In
this
study
conducted
over
two
years,
of
three
irrigation
levels
(moderate
deficit
with
65–75%
holding
capacity
threshold,
slight
75–85%,
sufficient
85–95%)
four
(60,
120,
240,
360
kg
ha−1)
growth,
yield,
productivities,
rhizosphere
microbial
diversities
functions
were
investigated.
The
results
demonstrated
that
highest
leaf
area,
dry
biomass,
productivities
obtained
under
treatment
irrigation.
With
increasing
application,
showed
a
trend
first
then
decreasing.
Overall,
(N2W3)
240
ha−1
N
was
productivities.
Moreover,
optimal
obviously
altered
structures
bacterial
fungal
communities,
particularly
recruiting
microbiota
conferring
benefits
to
growth
fixation—namely,
Lysobacter
Bradyrhizobium.
Ultimately,
significantly
increased
relative
abundances
related
carbon,
sulfur,
metabolism,
especially
fixation.
summary,
fertilization
enhanced
by
fixation
microbiome.
Our
provide
implications
for
cultivation
in
greenhouses,
terms
optimized
fertilization.
Язык: Английский
Endophytic bacteria: a sustainable strategy for enhancing medicinal plant cultivation and preserving microbial diversity
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 18, 2024
Endophytic
bacteria,
part
of
the
plant
microbiome,
hold
significant
potential
for
enhancing
cultivation
and
sustainability
medicinal
plants
(MPs).
These
microbes
are
integral
to
many
functions,
including
growth
promotion,
nutrient
acquisition,
resistance
biotic
abiotic
stresses.
However,
traditional
practices
often
overlook
importance
these
beneficial
microbes,
leading
reduced
crop
yields,
lower
phytochemical
quality,
increased
susceptibility
diseases.
The
domestication
MPs
use
chemical
fertilizers
disrupt
natural
microbial
diversity
in
soils,
essential
health
productivity
plants.
This
disruption
can
lead
loss
plant–microbe
interactions,
which
vital
production
bioactive
compounds
with
therapeutic
properties.
Recent
advances
microbiome
research,
supported
by
omics
technologies,
have
expanded
our
understanding
how
endophytic
bacteria
be
leveraged
enhance
MP
quality.
directly
boost
promoting
or
indirectly
restoring
healthy
soil
microbiomes.
They
also
harnessed
as
factories
produce
valuable
compounds,
either
transforming
plant-derived
precursors
into
substances
synthesizing
unique
metabolites
that
mimic
secondary
metabolites.
offers
a
sustainable
low-cost
alternative
cultivation,
reducing
carbon
footprint
preserving
endangered
species.
In
conclusion,
integrating
research
agricultural
could
revolutionize
cultivation.
By
focusing
on
component,
particularly
endophytes,
we
develop
more
productive
methods
cultivating
plants,
ultimately
contributing
biodiversity
conservation
high-value
products.
Язык: Английский
Potential plant benefits of endophytic microorganisms associated with halophyte <i>Glycyrrhiza glabra</i> L
AIMS Microbiology,
Год журнала:
2024,
Номер
10(4), С. 859 - 879
Опубликована: Янв. 1, 2024
<p>In
this
study,
bacteria
associated
with
licorice
(<italic>Glycyrrhiza
glabra</italic>
L.)
were
characterized
through
16S
rRNA
gene
analysis.
Profiling
of
endophytic
isolated
from
<italic>Glycyrrhiza
tissues
revealed
18
isolates
across
the
following
genera:
<italic>Enterobacter</italic>
(4),
<italic>Pantoea</italic>
(3),
<italic>Bacillus</italic>
(2),
<italic>Paenibacillus</italic>
<italic>Achromobacter</italic>
<italic>Pseudomonas</italic>
(1),
<italic>Escherichia</italic>
<italic>Klebsiella</italic>
<italic>Citrobacter</italic>
and
<italic>Kosakonia</italic>
(1).
Furthermore,
beneficial
features
bacterial
for
plants
determined.
The
showed
capacity
to
produce
siderophores,
hydrogen
cyanide
(HCN),
indole-3-acetic
acid
(IAA),
chitinase,
protease,
glucanase,
lipase,
other
enzymes.
Seven
antagonistic
activity
against
<italic>F.
culmorum</italic>,
solani</italic>,
<italic>R.
solani</italic>.
According
these
results,
antimicrobial
properties
may
serve
as
a
source
selection
microorganisms
that
have
plant
fungal
pathogens
be
considered
potential
candidates
control
pathogens.
selected
isolates,
<italic>P.
polymyxa</italic>
GU1,
<italic>A.
xylosoxidans</italic>
GU6,
azotoformans</italic>
GU7,
agglomerans</italic>
GU18,
increased
root
shoot
growth
able
colonize
root.
They
can
also
an
active
part
bioinoculants,
improving
growth.</p>
Язык: Английский