Single Particle Analysis-Enhanced DNA Walking Machine for Sensitive miRNA Detection

Analytical Chemistry, Год журнала: 2024, Номер 96(28), С. 11566 - 11571

Опубликована: Июнь 28, 2024

DNA walking machines have achieved significant breakthroughs in areas such as biosensing, bioimaging, and early cancer diagnosis, facilitated by the self-assembly of or its combination with other materials, magnetic beads metal nanoparticles. However, current machine strategies are constantly challenged inadequate analytical sensitivity, while sophisticated signal amplification procedures often indispensable. Single-particle inductively coupled plasma mass spectrometry (SP-ICPMS) provides superior sensitivity can effectively discriminate between background noise detected signals due to large number atoms a nanoparticle concentrating effect single detection. In this study, we present novel approach utilizing counting duplex-specific nuclease (DSN)-assisted construct 3D for detecting aggressive prostate (PCa) biomarker miRNA-200c. The proposed strategy showed an improvement detection limit (LOD) 0.93 pM (28 amol) was successfully applied human serum samples. To best our knowledge, is first report study.

Язык: Английский

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Electrochemical Sensors: Types, Applications, and the Novel Impacts of Vibration and Fluid Flow for Microfluidic Integration

Biosensors and Bioelectronics, Год журнала: 2025, Номер 272, С. 117099 - 117099

Опубликована: Янв. 2, 2025

Язык: Английский

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An electrochemical biosensor based on a novel nucleic acid isothermal amplification method for detecting pathogenic bacteria

Microchemical Journal, Год журнала: 2025, Номер unknown, С. 113396 - 113396

Опубликована: Март 1, 2025

Язык: Английский

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Redefining nanobiosensors in rapid detection of viral infections: Where are we now?

Trends in Environmental Analytical Chemistry, Год журнала: 2025, Номер unknown, С. e00263 - e00263

Опубликована: Март 1, 2025

Язык: Английский

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A lateral flow biosensor based on duplex-specific nuclease and novel isothermal amplification for detection of influenza virus

Enzyme and Microbial Technology, Год журнала: 2025, Номер 188, С. 110653 - 110653

Опубликована: Апрель 8, 2025

Язык: Английский

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Recent Advances in Enzyme‐Mediated Rolling Circle Replication Technology Empowering Virus Detection

Chinese Journal of Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 11, 2025

Comprehensive Summary The prevalence and spread of viral infectious diseases pose a grave threat to global public health, particularly resulting in significant number casualties. To curb the diseases, virus testing is one efficient economical means, among which nucleic acid detection has advantage detecting infections at an early stage, even before symptoms appear. Rolling circle replication representative enzyme‐mediated isothermal amplification technology, characterized by its mild reaction conditions, no need for temperature control equipment, high efficiency. This review provides conceptual overview latest advances rolling (RCR) their applications detection, focusing on molecular design principles different application scenarios. first part briefly describes significance advantages RCR detection. second elaborates principle preparation strategy derivative technologies. third focuses various scenarios In end, we provide perspective innovation improving accuracy specificity cope with challenges that may arise future.

Язык: Английский

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Novel Sensing Strategy for MicroRNA via DSN–RCA Dual Amplification and Optical Tweezer-Assisted Suspension Bead Arrays

Analytical Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 18, 2025

Traditional microRNA detection methods have some limitations that cannot be ignored. Enzyme-catalyzed nucleic acid amplification has gradually become an important method in bioanalysis and is expected to a substitute for traditional methods. Combining enzymes with different functions provides wide range of analytical design possibilities create high-performance sensing strategies. Meanwhile, the optical tweezers produce tightly focused laser beam are integrated suspension bead array fully concentrates signal labels, contributing stable output improved efficiency. Herein, we developed novel "DSN-RCA dual tweezer-assisted imaging" strategy highly efficient microRNA-21 (miRNA-21). The conversion low abundance targets into bulk fluorescence probes was achieved by duplex-specific nuclease (DSN), enrichment on supports rather than dispersion solution realized rolling circle (RCA) one step. synergistic integration application effectively sensitivity specificity. Finally, created modified tweezer system, which high-quality imaging analysis proposed limit (LOD) as 5.90 pM excellent specificity, stability, reproducibility successfully applied human serum samples, great potential clinical serological research.

Язык: Английский

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An Fe₃O₄-Au heterodimer nanoparticle-based lateral flow assay for rapid and simultaneous detection of multiple influenza virus nucleic acids

Analytical Methods, Год журнала: 2024, Номер 16(34), С. 5777 - 5784

Опубликована: Янв. 1, 2024

An Fe 3 O 4 -Au nanoparticle-based LFA was developed for simultaneous detection of multiple influenza virus nucleic acids. This showed good accuracy and satisfactory sensitivity, could serve as a portable method early detection.

Язык: Английский

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Label-free Multiplexed Detection Based on Core–Shell Photonic Barcodes integrated RCA

Biosensors and Bioelectronics, Год журнала: 2024, Номер 271, С. 117037 - 117037

Опубликована: Дек. 6, 2024

Язык: Английский

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Sensitive detection of exosomes by an electrochemical aptasensor based on double nucleic acid amplifications

Microchemical Journal, Год журнала: 2024, Номер 201, С. 110667 - 110667

Опубликована: Май 1, 2024

Язык: Английский

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