Contributions of Microscopy to the Morphological Characterization of the Male Genitalia of Toxomerus politus (Diptera, Syrphidae)
Ecology and Evolution,
Год журнала:
2025,
Номер
15(2)
Опубликована: Фев. 1, 2025
ABSTRACT
The
study
of
insects
has
historically
been
linked
to
the
development
microscopy.
As
techniques
have
improved,
research
into
insect
morphology
can
increasingly
contribute
a
better
understanding
structures
and
functions
morphological
characters
insects'
three
tagmata.
We
propose
use
methodologies
applied
in
microscopy
as
way
improving
facilitating
fly
genitalia.
In
addition,
flies
preserved
70%
alcohol
were
used
verify
feasibility
distinguishing
ultrastructural
spermatozoa,
when
not
previously
fixed
for
preservation
structures.
shown
that
it
is
viable
enhance
specimens
using
scanning
electron
technique.
observation
genitalia
this
technique,
together
with
confocal
laser
microscopy,
enables
visualization,
description,
characteristics
also
noted,
analysis
transmission
possible
recover
describe
some
features
ultrastructure
Toxomerus
politus
(Say,
1823)
sperm,
obtained
from
stored
long
period
time.
Comparison
between
methods
investigate
T.
,
observed
after
preserving
specimen,
already
demonstrates
importance
appropriate
methodology
starting
point
reliable
results.
adopted
work
potential
be
extrapolated
other
species
broad
context.
Язык: Английский
Fine structure of the female genital system of diving beetle Stictonectes optatus (Seidlitz, 1887) (Dytiscidae-Hydroporinae) and evidence of mating plug formation
Arthropod Structure & Development,
Год журнала:
2023,
Номер
73, С. 101250 - 101250
Опубликована: Март 1, 2023
The
general
organization
of
the
female
genital
system
diving
beetle
Stictonectes
optatus
was
studied,
clarifying
complex
structure
spermatheca
and
spermathecal
gland.
two
structures
adhere
closely
to
each
other,
sharing
a
small
area
their
cuticular
epithelium.
A
long
duct
connects
bursa
copulatrix
spermatheca,
where
sperm
are
stored.
reach
common
oviduct,
egg
fertilization
occurs,
via
duct.
gland
cells
have
extracellular
cisterns
secretions
Thin
ducts
composed
duct-forming
transport
these
apical
region
into
lumen.
Soon
after
mating,
is
almost
completely
occupied
by
plug
secreted
male
accessory
glands.
epithelium
seem
contribute
formation.
Later
this
becomes
large
spherical,
obstructing
copulatrix.
Язык: Английский
Proteomic diversification of spermatostyles among six species of whirligig beetles
Molecular Reproduction and Development,
Год журнала:
2024,
Номер
91(5)
Опубликована: Май 1, 2024
Abstract
Seminal
fluid
protein
composition
is
complex
and
commonly
assumed
to
be
rapidly
divergent
due
functional
interactions
with
both
sperm
the
female
reproductive
tract
(FRT),
of
which
evolve
rapidly.
In
addition
sperm,
seminal
may
contain
structures,
such
as
mating
plugs
spermatophores.
Here,
we
investigate
evolutionary
diversification
a
lesser‐known
ejaculate
structure:
spermatostyle,
has
independently
arisen
in
several
families
beetles
true
bugs.
We
characterized
spermatostyle
proteome,
FRT
morphology,
six
species
whirligig
(family
Gyrinidae).
Spermatostyles
were
enriched
for
proteolytic
enzymes,
assays
confirmed
they
possess
activity.
Sperm‐leucylaminopeptidases
(S‐LAPs)
particularly
abundant,
their
localization
spermatostyles
was
by
immunohistochemistry.
Although
there
evidence
conservation
proteomes
across
species,
phylogenetic
regressions
suggest
covariation
between
morphology
FRTs.
postulate
that
S‐LAPs
(and
other
proteases)
have
evolved
novel
structural
role
discuss
adaptations
delivering
male‐derived
materials
females.
Язык: Английский
The Structure of the Female Genital System of the Diving Beetle Scarodytes halensis (Fabricius, 1787) (Hydroporinae, Dytiscidae), and the Organization of the Spermatheca and the Spermathecal Gland Complex
Insects,
Год журнала:
2023,
Номер
14(3), С. 282 - 282
Опубликована: Март 13, 2023
The
fine
structure
of
the
female
reproductive
organs
diving
beetle
Scarodytes
halensis
has
been
described,
with
particular
attention
to
complex
organization
spermatheca
and
spermathecal
gland.
These
are
fused
in
a
single
whose
epithelium
is
involved
quite
different
activity.
secretory
cells
gland
have
large
extracellular
cistern
secretions;
duct-forming
cells,
by
their
efferent
duct,
transport
secretions
up
apical
cell
region
where
they
discharged
into
lumen.
On
contrary,
spermatheca,
filled
sperm,
simple
epithelium,
apparently
not
ultrastructure
almost
identical
that
described
closely
related
species
Stictonectes
optatus.
Sc.
long
duct
connecting
bursa
copulatrix
spermatheca-spermathecal
complex.
This
thick
outer
layer
muscle
cells.
Through
contractions,
sperm
can
be
pushed
forwarding
two
organs.
A
short
fertilization
allows
reach
common
oviduct
eggs
will
fertilized.
genital
systems
S.
optatus
might
strategy
species.
Язык: Английский
Sperm structure of the diving beetle Deronectes moestus incospectus (Leprieur, 1876) (Hydroporinae, Dytiscidae) and considerations on extracellular material surrounding sperm bundles
Micron,
Год журнала:
2023,
Номер
171, С. 103484 - 103484
Опубликована: Май 13, 2023
The
sperm
cells
of
the
diving
beetle
Deronectes
moestus
incospectus
are
characterized
by
conjugation
leading
to
formation
bundles
64
units
each.
These
formed
at
end
spermatocyte
cell
divisions
occurring
in
testes
and
can
be
detected
anterior
region
deferent
ducts
(first
type
conjugation).
Fusions
some
occur
ducts.
show
sperm-head
stacks
(sperm
rouleaux)
surrounded
a
cup
extracellular
material
secreted
epithelial
This
extends
posteriorly
around
bundle
cover
nuclei
initial
flagella.
consists
fine
tubules,
is
no
longer
visible
posterior
D.
have
an
axoneme
with
9
+
2
pattern
unusual
mitochondrial
derivatives
having
matrix
showing
dense
dots
small
crystallized
domain.
Two
thin
elongated
accessory
bodies
located
between
axoneme.
different
morphologies
various
families
Adephaga,
but
all
produced
epithelium
Thus
it
reasonable
assume
that
has
same
function
groups.
Язык: Английский
Morphology of the Female and Male Reproductive Tracts and More Data on the Spermatostyle in the Brazilian Gyretes sp. (Coleoptera, Adephaga, Gyrinidae)
Microscopy and Microanalysis,
Год журнала:
2023,
Номер
29(6), С. 2184 - 2203
Опубликована: Ноя. 22, 2023
We
investigated
the
male
and
female
reproductive
tracts
of
Gyretes
sp.
with
light
transmission
electron
microscopies.
The
has
a
pair
testes
single
coiled
follicle,
followed
by
short
efferent
ducts,
which
have
similar
shape
diameter
to
testes.
Long
ducts
(epididymides)
differential
epithelium
open
in
long
vasa
deferentia
that
lead
accessory
glands.
Glycoprotein
secretions
from
vas
deferens
constitute
spermatostyle
for
spermatozoa
aggregation.
numerous
ovarioles
per
ovary,
fertilization
duct,
an
gland,
elongated
vagina.
Spermatozoa
are
stored
as
unaggregated
cells
duct.
In
Gyrinidae,
glands
show
diverse
shapes,
sperm
storage
organs
vary
shape,
size,
type
may
play
role
interaction
aggregates.
Testes
follicle
opening
features
shared
other
Gyrinidae
Adephaga.
proposed
adding
this
latter
trait
characterize
suborder
beetles.
morphology
both
sexes
contributes
comparative
analyses
knowledge
biology
provide
additional
systematics.
Язык: Английский
The Cap and the Spermatostyle Protecting the Sperm Bundle Have a Similar Origin—Ultrastructural Study of the Spermatogenesis from the Ground Beetle Carabus (Chaetocarabus) lefebvrei Dejean, 1826 (Adephaga Carabidae)
Insects,
Год журнала:
2024,
Номер
15(11), С. 864 - 864
Опубликована: Ноя. 5, 2024
The
males
of
Язык: Английский
Ultrastructure of the Female Reproductive Organs of the Diving Beetle Deronectes Moestus Incospectus (Leprieur, 1876) (Dytiscidae, Hydroporinae)
Опубликована: Янв. 1, 2023
We
describe
the
ultrastructure
of
female
reproductive
organs
Deronectes
moestus
(Dytiscidae
Hydroporinae).
The
long
spermathecal
duct
has
a
simple
epithelium
lined
with
thin
cuticle
and
surrounded
by
thick
layer
muscle
fibres.
wide
lumen
contains
electron-dense
secretory
material,
among
which
remnants
extracellular
material
are
visible.
This
consists
tubular
structures
assembled
around
sperm
bundles
previously
described
in
male
deferent
ducts.
so-called
gland,
disposed
along
duct,
is
structure
epithelial
cells
an
irregular
bearing
rich
system
microvilli.
Many
mitochondria
visible
apical
cytoplasm
cells,
few
spheroidal
bodies
close
to
basal
nuclei.
Since
gland
suggests
it
involved
fluid
uptake
from
rather
than
activity,
term
coined
other
authors
this
organ,
inappropriate.
spermatheca
large
complex
showing
duct-forming
cells.
organ
distinctive
ultrastructural
features
those
ducts,
namely
having
mitochondrial
matrix
small
crystallized
area
dots.
Because
its
overall
organization,
D.
can
be
considered
more
integrated
studied
hydroporine
species.
Язык: Английский
Ultrastructure of the female reproductive organs of the diving beetle Deronectes moestus incospectus (Leprieur, 1876) (Dytiscidae, Hydroporinae)
Arthropod Structure & Development,
Год журнала:
2023,
Номер
75, С. 101287 - 101287
Опубликована: Июль 1, 2023
We
describe
the
ultrastructure
of
female
reproductive
organs
Deronectes
moestus
(Dytiscidae
Hydroporinae).
The
long
spermathecal
duct
has
a
simple
epithelium
lined
internally
by
thin
cuticle
and
externally
thick
layer
muscle
cells.
wide
lumen
contains
electron-dense
material,
among
which
remnants
extracellular
material
are
visible.
This
consists
tubular
structures
assembled
around
sperm
bundles
previously
described
in
male
deferent
ducts.
so-called
gland,
disposed
along
duct,
is
structure
with
epithelial
cells
an
irregular
bearing
rich
system
microvilli.
Many
mitochondria
visible
apical
cytoplasm
cells,
few
spheroidal
bodies
close
to
basal
nuclei.
Since
gland
suggests
it
involved
fluid
uptake
from
rather
than
secretory
activity,
term
coined
other
authors
this
organ,
inappropriate.
spermatheca
large
complex
showing
duct-forming
organ
distinctive
ultrastructural
features
those
ducts,
namely
having
mitochondrial
matrix
small
crystallized
area
dots.
Because
its
overall
organization,
D.
can
be
considered
more
integrated
studied
hydroporine
species.
Язык: Английский